{"id":"https://openalex.org/W2085386509","doi":"https://doi.org/10.1186/1472-6750-9-33","title":"Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function","display_name":"Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2085386509","doi":"https://doi.org/10.1186/1472-6750-9-33","mag":"2085386509","pmid":"https://pubmed.ncbi.nlm.nih.gov/19344517","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2678116"},"language":"en","primary_location":{"id":"doi:10.1186/1472-6750-9-33","doi":"https://doi.org/10.1186/1472-6750-9-33","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-9-33","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-33","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-33","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078620429","display_name":"Henry Daniell","orcid":"https://orcid.org/0000-0003-4485-1176","observed_orcids":["https://orcid.org/0000-0003-4485-1176"]},"institutions":[{"id":"https://openalex.org/I106165777","display_name":"University of Central Florida","ror":"https://ror.org/036nfer12","country_code":"US","type":"education","lineage":["https://openalex.org/I106165777"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Henry Daniell","raw_affiliation_strings":["Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Orlando, FL 32816-2364, USA. daniell@mail.ucf.edu","Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Orlando, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Orlando, FL 32816-2364, USA. daniell@mail.ucf.edu","institution_ids":["https://openalex.org/I106165777"]},{"raw_affiliation_string":"Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Orlando, USA","institution_ids":["https://openalex.org/I106165777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033101160","display_name":"Gricel Ruiz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106165777","display_name":"University of Central Florida","ror":"https://ror.org/036nfer12","country_code":"US","type":"education","lineage":["https://openalex.org/I106165777"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gricel Ruiz","raw_affiliation_strings":["Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Room 336, Orlando, FL, 32816-2364, USA","Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Orlando, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Room 336, Orlando, FL, 32816-2364, USA","institution_ids":["https://openalex.org/I106165777"]},{"raw_affiliation_string":"Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Biomolecular Science Building, Orlando, USA","institution_ids":["https://openalex.org/I106165777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075481172","display_name":"Bela Stephen Denes","orcid":"https://orcid.org/0000-0002-9889-529X","observed_orcids":["https://orcid.org/0000-0002-9889-529X"]},"institutions":[{"id":"https://openalex.org/I26347476","display_name":"Loma Linda University","ror":"https://ror.org/04bj28v14","country_code":"US","type":"education","lineage":["https://openalex.org/I26347476"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bela Denes","raw_affiliation_strings":["Center for Health Disparities and Molecular Medicine, Loma Linda University, 161 Mortensen Hall, Loma Linda, CA, 92350, USA","Center for Health Disparities and Molecular Medicine, Loma Linda University, Loma Linda, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Health Disparities and Molecular Medicine, Loma Linda University, 161 Mortensen Hall, Loma Linda, CA, 92350, USA","institution_ids":["https://openalex.org/I26347476"]},{"raw_affiliation_string":"Center for Health Disparities and Molecular Medicine, Loma Linda University, Loma Linda, USA","institution_ids":["https://openalex.org/I26347476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052453983","display_name":"Laurence Sandberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I26347476","display_name":"Loma Linda University","ror":"https://ror.org/04bj28v14","country_code":"US","type":"education","lineage":["https://openalex.org/I26347476"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Laurence Sandberg","raw_affiliation_strings":["Department of Biochemistry, Loma Linda University, Loma Linda, CA, 92350, USA","Department of Biochemistry, Loma Linda University, Loma Linda, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Loma Linda University, Loma Linda, CA, 92350, USA","institution_ids":["https://openalex.org/I26347476"]},{"raw_affiliation_string":"Department of Biochemistry, Loma Linda University, Loma Linda, USA","institution_ids":["https://openalex.org/I26347476"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075757369","display_name":"William H. R. Langridge","orcid":"https://orcid.org/0000-0002-7636-7349","observed_orcids":["https://orcid.org/0000-0002-7636-7349"]},"institutions":[{"id":"https://openalex.org/I26347476","display_name":"Loma Linda University","ror":"https://ror.org/04bj28v14","country_code":"US","type":"education","lineage":["https://openalex.org/I26347476"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William Langridge","raw_affiliation_strings":["Center for Health Disparities and Molecular Medicine, Loma Linda University, 161 Mortensen Hall, Loma Linda, CA, 92350, USA","Loma Linda University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Health Disparities and Molecular Medicine, Loma Linda University, 161 Mortensen Hall, Loma Linda, CA, 92350, USA","institution_ids":["https://openalex.org/I26347476"]},{"raw_affiliation_string":"Loma Linda University","institution_ids":["https://openalex.org/I26347476"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5078620429"],"corresponding_institution_ids":["https://openalex.org/I106165777"],"apc_list":{"value":2990,"currency":"USD","value_usd":2990},"apc_paid":{"value":2990,"currency":"USD","value_usd":2990},"fwci":8.8055,"has_fulltext":true,"cited_by_count":92,"citation_normalized_percentile":{"value":0.99127856,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"9","issue":"1","first_page":"33","last_page":"33"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-like-growth-factor-i","display_name":"insulin-like growth factor I","score":0.8450000286102295},{"id":"https://openalex.org/keywords/codon-optimization","display_name":"codon optimization","score":0.9190000295639038},{"id":"https://openalex.org/keywords/regulatory-elements","display_name":"regulatory elements","score":0.5960000157356262},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8629999756813049},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.4729999899864197},{"id":"https://openalex.org/keywords/transgene-containment","display_name":"transgene containment","score":0.6069999933242798}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8462268114089966},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6793040037155151},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.649429440498352},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4742547571659088},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43505001068115234},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.340868204832077}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007334","descriptor_name":"Insulin-Like Growth Factor I","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D007334","descriptor_name":"Insulin-Like Growth Factor I","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D012045","descriptor_name":"Regulatory Sequences, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1186/1472-6750-9-33","doi":"https://doi.org/10.1186/1472-6750-9-33","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-9-33","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-33","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},{"id":"pmid:19344517","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19344517","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2678116","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2678116/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnol","raw_type":"Text"},{"id":"pmh:oai:repository.upenn.edu:20.500.14332/9053","doi":null,"is_oa":false,"landing_page_url":"https://repository.upenn.edu/handle/20.500.14332/9053","pdf_url":null,"source":{"id":"https://openalex.org/S4306402083","display_name":"ScholarlyCommons (University of Pennsylvania)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79576946","host_organization_name":"University of Pennsylvania","host_organization_lineage":["https://openalex.org/I79576946"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"9","raw_type":"Article"},{"id":"pmh:oai:repository.upenn.edu:dental_papers-1111","doi":null,"is_oa":false,"landing_page_url":"https://repository.upenn.edu/dental_papers/327","pdf_url":null,"source":{"id":"https://openalex.org/S4306402083","display_name":"ScholarlyCommons (University of Pennsylvania)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79576946","host_organization_name":"University of Pennsylvania","host_organization_lineage":["https://openalex.org/I79576946"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Departmental Papers (Dental)","raw_type":"text"},{"id":"pmh:oai:stars.library.ucf.edu:facultybib2000-2448","doi":null,"is_oa":true,"landing_page_url":"https://stars.library.ucf.edu/facultybib2000/1449","pdf_url":null,"source":{"id":"https://openalex.org/S4210172555","display_name":"Journal of International Crisis and Risk Communication Research","issn_l":"2576-0017","issn":["2576-0017","2576-0025"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","vabb-shw"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Bibliography 2000s","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:a3a314ab8e9b446799516255085b93b6","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a3a314ab8e9b446799516255085b93b6","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Biotechnology, Vol 9, Iss 1, p 33 (2009)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/1472-6750-9-33","doi":"https://doi.org/10.1186/1472-6750-9-33","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-9-33","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-33","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2755920597","display_name":"Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts","funder_award_id":"R01GM063879","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3611247453","display_name":null,"funder_award_id":"R01GM","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G5520316217","display_name":null,"funder_award_id":"NIH-R01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320322649","display_name":"Universidad de Navarra","ror":"https://ror.org/02rxc7m23"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2085386509.pdf","grobid_xml":"https://content.openalex.org/works/W2085386509.grobid-xml"},"referenced_works_count":57,"referenced_works":["https://openalex.org/W207050664","https://openalex.org/W219020719","https://openalex.org/W1214533475","https://openalex.org/W1509665269","https://openalex.org/W1524733670","https://openalex.org/W1572066007","https://openalex.org/W1606565747","https://openalex.org/W1965035321","https://openalex.org/W1967989396","https://openalex.org/W1971775793","https://openalex.org/W1977294998","https://openalex.org/W1981284608","https://openalex.org/W1983181891","https://openalex.org/W1992050856","https://openalex.org/W1992686283","https://openalex.org/W2001532511","https://openalex.org/W2002551743","https://openalex.org/W2016937966","https://openalex.org/W2020497113","https://openalex.org/W2023327810","https://openalex.org/W2025349200","https://openalex.org/W2026386446","https://openalex.org/W2027945375","https://openalex.org/W2028234655","https://openalex.org/W2036949542","https://openalex.org/W2048224470","https://openalex.org/W2049620495","https://openalex.org/W2051846416","https://openalex.org/W2060094399","https://openalex.org/W2061082318","https://openalex.org/W2062452411","https://openalex.org/W2072514327","https://openalex.org/W2075196606","https://openalex.org/W2086947027","https://openalex.org/W2087274225","https://openalex.org/W2089653069","https://openalex.org/W2090408737","https://openalex.org/W2104742235","https://openalex.org/W2105433121","https://openalex.org/W2107683452","https://openalex.org/W2121377693","https://openalex.org/W2123793880","https://openalex.org/W2125297184","https://openalex.org/W2128201614","https://openalex.org/W2132170882","https://openalex.org/W2133723912","https://openalex.org/W2142297287","https://openalex.org/W2143349729","https://openalex.org/W2159164339","https://openalex.org/W2162291940","https://openalex.org/W2163866049","https://openalex.org/W2164948283","https://openalex.org/W2172029739","https://openalex.org/W2409722157","https://openalex.org/W2416206049","https://openalex.org/W2503430986","https://openalex.org/W4237275811"],"related_works":["https://openalex.org/W3210720526","https://openalex.org/W2751466048","https://openalex.org/W2737914188","https://openalex.org/W2048224470","https://openalex.org/W7000396597","https://openalex.org/W1514758690","https://openalex.org/W3171294543","https://openalex.org/W4313656285","https://openalex.org/W219020719","https://openalex.org/W2128201614"],"abstract_inverted_index":{"BACKGROUND:":[0],"Transgenic":[1],"chloroplasts":[2,49,273,289],"are":[3],"potential":[4],"bioreactors":[5,291],"for":[6,11,50],"recombinant":[7],"protein":[8,17,134,279],"production,":[9],"especially":[10],"achievement":[12],"of":[13,16,23,33,52,59,97,129,131,163,185,206,226,245,253,295],"high":[14],"levels":[15,128],"expression":[18,127,162],"and":[19,55,159,194,199,229,280,307],"proper":[20],"folding.":[21],"Production":[22],"therapeutic":[24],"proteins":[25,294],"in":[26,37,47,73,88,92,156,209,237,250,271],"leaves":[27],"provides":[28],"transgene":[29],"containment":[30],"by":[31,174],"elimination":[32],"reproductive":[34],"structures.":[35],"Therefore,":[36],"this":[38],"study,":[39],"human":[40,207,227,238,265],"Insulin":[41,62,266],"like":[42,63,267],"Growth":[43,64,268],"Factor-1":[44,269],"is":[45,150,274,281,304],"expressed":[46,270],"transgenic":[48,117,122,210,272],"evaluation":[51],"structural":[53],"identity":[54,205],"function.":[56],"RESULTS:":[57],"Expression":[58],"the":[60,89,98,101,116,146,175,204,221,230,242,251,254,264,277],"synthetic":[61],"Factor":[65],"1":[66],"gene":[67,81],"(IGF-1s,":[68],"60%":[69],"AT)":[70,84],"was":[71,86,105,165],"observed":[72],"transformed":[74],"E.":[75,93,154],"coli.":[76,94],"However,":[77],"no":[78],"native":[79,278],"IGF-1":[80,164,191,208,228,248],"(IGF-1n,":[82],"41%":[83],"product":[85],"detected":[87],"western":[90],"blots":[91],"Site-specific":[95],"integration":[96],"transgenes":[99],"into":[100],"tobacco":[102],"chloroplast":[103,147,176,189,246],"genome":[104],"confirmed":[106,114,203],"after":[107],"transformation":[108],"using":[109],"PCR.":[110],"Southern":[111],"blot":[112],"analysis":[113,202],"that":[115,145,263,299,310],"lines":[118,124],"were":[119],"homoplasmic.":[120],"The":[121,136,161,284],"plant":[123,288],"had":[125],"IGF-1s":[126],"11.3%":[130],"total":[132],"soluble":[133],"(TSP).":[135],"IGF-1n":[137],"plants":[138],"contained":[139],"9.5%":[140],"TSP":[141,170],"as":[142,290],"IGF-1n,":[143],"suggesting":[144],"translation":[148],"machinery":[149],"more":[151],"flexible":[152],"than":[153],"coli":[155],"codon":[157],"preference":[158],"usage.":[160],"increased":[166],"up":[167],"to":[168,276,286,292,312],"32%":[169],"under":[171],"continuous":[172],"illumination":[173],"light":[177],"regulatory":[178],"elements.":[179],"IgG-Sepharose":[180],"affinity":[181],"column":[182],"chromatographic":[183],"separation":[184],"Z":[186,257],"domain":[187],"containing":[188],"derived":[190,247],"protein,":[192],"single":[193],"two":[195],"dimensional":[196],"electrophoresis":[197],"methods":[198],"mass":[200],"spectrometer":[201],"chloroplasts.":[211],"Two":[212],"spots":[213],"analyzed":[214],"from":[215],"2-D":[216],"focusing/phoresis":[217],"acrylamide":[218],"gel":[219],"showed":[220],"correct":[222],"amino":[223],"acid":[224],"sequence":[225],"S.":[231,255],"aureus":[232,256],"Z-tag.":[233],"Cell":[234],"proliferation":[235],"assays":[236],"HU-3":[239],"cells":[240],"demonstrated":[241],"biological":[243,302],"activity":[244,303],"even":[249],"presence":[252],"tag.":[258],"CONCLUSION:":[259],"This":[260],"study":[261],"demonstrates":[262],"identical":[275],"fully":[282],"functional.":[283],"ability":[285],"use":[287],"generate":[293],"great":[296],"economic":[297],"value":[298],"retain":[300],"their":[301],"an":[305],"exciting":[306],"achievable":[308],"goal":[309],"appears":[311],"be":[313],"within":[314],"our":[315],"grasp.":[316]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}