{"id":"https://openalex.org/W208402615","doi":"https://doi.org/10.1023/a:1020655701852","title":"In Vitro Translation of Type A Clostridium botulinum Neurotoxin Heavy Chain and Analysis of Its Binding to Rat Synaptosomes","display_name":"In Vitro Translation of Type A Clostridium botulinum Neurotoxin Heavy Chain and Analysis of Its Binding to Rat Synaptosomes","publication_year":1999,"publication_date":"1999-01-01","ids":{"openalex":"https://openalex.org/W208402615","doi":"https://doi.org/10.1023/a:1020655701852","mag":"208402615","pmid":"https://pubmed.ncbi.nlm.nih.gov/10071933"},"language":"en","primary_location":{"id":"doi:10.1023/a:1020655701852","doi":"https://doi.org/10.1023/a:1020655701852","is_oa":false,"landing_page_url":"https://doi.org/10.1023/a:1020655701852","pdf_url":null,"source":{"id":"https://openalex.org/S68211258","display_name":"Journal of Protein Chemistry","issn_l":"0277-8033","issn":["0277-8033","1573-4943"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Protein Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100361080","display_name":"Li Li","orcid":"https://orcid.org/0000-0002-0140-1995","observed_orcids":["https://orcid.org/0000-0002-0140-1995"]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Li Li","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 02747, USA","institution_ids":["https://openalex.org/I100633361"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083903249","display_name":"Bal Ram Singh","orcid":"https://orcid.org/0000-0002-9069-1487","observed_orcids":["https://orcid.org/0000-0002-9069-1487"]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bal Ram Singh","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth, Massachusetts, 02747","Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth, Massachusetts, 02747","institution_ids":["https://openalex.org/I100633361"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth","institution_ids":["https://openalex.org/I100633361"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I100633361"],"apc_list":{"value":3890,"currency":"USD","value_usd":3890},"apc_paid":null,"fwci":1.1919,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.76667655,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"18","issue":"1","first_page":"89","last_page":"95"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11353","display_name":"Venomous Animal Envenomation and Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/botulinum-toxin-type-a","display_name":"botulinum toxin type A","score":0.9409999847412109},{"id":"https://openalex.org/keywords/heavy-chain","display_name":"heavy chain","score":0.8790000081062317},{"id":"https://openalex.org/keywords/in-vitro-translation","display_name":"in vitro translation","score":0.9369999766349792},{"id":"https://openalex.org/keywords/cholinergic-synapses","display_name":"cholinergic synapses","score":0.7490000128746033},{"id":"https://openalex.org/keywords/neurotransmitter-release-inhibition","display_name":"neurotransmitter release inhibition","score":0.843999981880188},{"id":"https://openalex.org/keywords/toxin-binding-proteins","display_name":"toxin-binding proteins","score":0.503000020980835},{"id":"https://openalex.org/keywords/trypsin-sensitivity","display_name":"trypsin sensitivity","score":0.5509999990463257}],"concepts":[{"id":"https://openalex.org/C2777010668","wikidata":"https://www.wikidata.org/wiki/Q131268","display_name":"Clostridium botulinum","level":3,"score":0.8686636686325073},{"id":"https://openalex.org/C2780358027","wikidata":"https://www.wikidata.org/wiki/Q407752","display_name":"Neurotoxin","level":2,"score":0.565241813659668},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5533230900764465},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5269998908042908},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5254344344139099},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4964767098426819},{"id":"https://openalex.org/C36394416","wikidata":"https://www.wikidata.org/wiki/Q949267","display_name":"Immunoglobulin light chain","level":3,"score":0.49551188945770264},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.4497489929199219},{"id":"https://openalex.org/C2777367657","wikidata":"https://www.wikidata.org/wiki/Q184651","display_name":"Toxin","level":2,"score":0.43527504801750183},{"id":"https://openalex.org/C2778921374","wikidata":"https://www.wikidata.org/wiki/Q7662055","display_name":"Synaptosome","level":3,"score":0.4348057806491852},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.42121070623397827},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2987630367279053},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.23587605357170105},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1792934238910675},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012156","descriptor_name":"Reticulocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013574","descriptor_name":"Synaptosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1023/a:1020655701852","doi":"https://doi.org/10.1023/a:1020655701852","is_oa":false,"landing_page_url":"https://doi.org/10.1023/a:1020655701852","pdf_url":null,"source":{"id":"https://openalex.org/S68211258","display_name":"Journal of Protein Chemistry","issn_l":"0277-8033","issn":["0277-8033","1573-4943"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Protein Chemistry","raw_type":"journal-article"},{"id":"pmid:10071933","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10071933","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of protein chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4487037251","display_name":"MECHANISM OF BOTULINUM NEUROTOXIN ACTION","funder_award_id":"R29NS033740","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320315739","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1512697776","https://openalex.org/W1568588831","https://openalex.org/W1979084295","https://openalex.org/W1979290516","https://openalex.org/W1991480269","https://openalex.org/W2002775774","https://openalex.org/W2008234877","https://openalex.org/W2011748656","https://openalex.org/W2026755111","https://openalex.org/W2040375815","https://openalex.org/W2041201209","https://openalex.org/W2044080354","https://openalex.org/W2050672044","https://openalex.org/W2055787540","https://openalex.org/W2068277564","https://openalex.org/W2068379293","https://openalex.org/W2075449190","https://openalex.org/W2083953048","https://openalex.org/W2087719340","https://openalex.org/W2094886338","https://openalex.org/W2134886492","https://openalex.org/W2142036252","https://openalex.org/W4246786135","https://openalex.org/W4298048694","https://openalex.org/W4388243418"],"related_works":["https://openalex.org/W1967680616","https://openalex.org/W1997003449","https://openalex.org/W145299158","https://openalex.org/W2048788030","https://openalex.org/W2759365617","https://openalex.org/W2005816364","https://openalex.org/W2006500200","https://openalex.org/W1937892","https://openalex.org/W2411387082","https://openalex.org/W2030531542"],"abstract_inverted_index":null,"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}