{"id":"https://openalex.org/W2083538005","doi":"https://doi.org/10.2165/00066982-200509010-00004","title":"Touch-Down Reverse Transcriptase-PCR Detection of IgVH Rearrangement and Sybr-Green-Based Real-Time RT-PCR Quantitation of Minimal Residual Disease in Patients with Chronic Lymphocytic Leukemia","display_name":"Touch-Down Reverse Transcriptase-PCR Detection of IgVH Rearrangement and Sybr-Green-Based Real-Time RT-PCR Quantitation of Minimal Residual Disease in Patients with Chronic Lymphocytic Leukemia","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W2083538005","doi":"https://doi.org/10.2165/00066982-200509010-00004","mag":"2083538005","pmid":"https://pubmed.ncbi.nlm.nih.gov/16035732"},"language":"en","primary_location":{"id":"doi:10.2165/00066982-200509010-00004","doi":"https://doi.org/10.2165/00066982-200509010-00004","is_oa":false,"landing_page_url":"https://doi.org/10.2165/00066982-200509010-00004","pdf_url":null,"source":{"id":"https://openalex.org/S157311635","display_name":"Molecular Diagnosis","issn_l":"1084-8592","issn":["1084-8592","1532-8619"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core"],"host_organization":"https://openalex.org/P4310320330","host_organization_name":"Adis, Springer Healthcare","host_organization_lineage":["https://openalex.org/P4310320330","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Adis, Springer Healthcare","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Diagnosis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5096866459","display_name":"So a Pekov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210096318","display_name":"Institute of Haematology and Blood Transfusion","ror":"https://ror.org/00n6rde07","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210096318"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"So??a Pekov??","raw_affiliation_strings":["Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic","institution_ids":["https://openalex.org/I4210096318"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096866460","display_name":"Jana Markov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210096318","display_name":"Institute of Haematology and Blood Transfusion","ror":"https://ror.org/00n6rde07","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210096318"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Jana Markov??","raw_affiliation_strings":["Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic","institution_ids":["https://openalex.org/I4210096318"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033517685","display_name":"Petr Pajer","orcid":"https://orcid.org/0000-0002-8706-9371","observed_orcids":["https://orcid.org/0000-0002-8706-9371"]},"institutions":[{"id":"https://openalex.org/I4210138103","display_name":"Czech Academy of Sciences, Institute of Molecular Genetics","ror":"https://ror.org/045syc608","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Petr Pajer","raw_affiliation_strings":["Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210138103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096866461","display_name":"Michal Dvo k","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210138103","display_name":"Czech Academy of Sciences, Institute of Molecular Genetics","ror":"https://ror.org/045syc608","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Michal Dvo????k","raw_affiliation_strings":["Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210138103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096866462","display_name":"Petr Cetkovsk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210096318","display_name":"Institute of Haematology and Blood Transfusion","ror":"https://ror.org/00n6rde07","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210096318"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Petr Cetkovsk??","raw_affiliation_strings":["Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic","institution_ids":["https://openalex.org/I4210096318"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111843172","display_name":"J Schwarz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210096318","display_name":"Institute of Haematology and Blood Transfusion","ror":"https://ror.org/00n6rde07","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210096318"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Ji???? Schwarz","raw_affiliation_strings":["Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic","institution_ids":["https://openalex.org/I4210096318"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5096866459"],"corresponding_institution_ids":["https://openalex.org/I4210096318"],"apc_list":null,"apc_paid":null,"fwci":1.5331,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.82577598,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"9","issue":"1","first_page":"23","last_page":"34"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11157","display_name":"Chronic Lymphocytic Leukemia Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11157","display_name":"Chronic Lymphocytic Leukemia Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10950","display_name":"Acute Lymphoblastic Leukemia research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chronic-lymphocytic-leukemia","display_name":"chronic lymphocytic leukemia","score":0.9990000128746033},{"id":"https://openalex.org/keywords/measurable-residual-disease","display_name":"minimal residual disease","score":0.9940000176429749},{"id":"https://openalex.org/keywords/degenerate-primers","display_name":"degenerate primers","score":0.7770000100135803},{"id":"https://openalex.org/keywords/autologous-stem-cell-transplantation","display_name":"autologous stem cell transplantation","score":0.8629999756813049},{"id":"https://openalex.org/keywords/relapse-detection","display_name":"relapse detection","score":0.6370000243186951},{"id":"https://openalex.org/keywords/molecular-remission","display_name":"molecular remission","score":0.7369999885559082}],"concepts":[{"id":"https://openalex.org/C2779823535","wikidata":"https://www.wikidata.org/wiki/Q6865356","display_name":"Minimal residual disease","level":3,"score":0.8832209706306458},{"id":"https://openalex.org/C2780968160","wikidata":"https://www.wikidata.org/wiki/Q423692","display_name":"SYBR Green I","level":4,"score":0.5985748171806335},{"id":"https://openalex.org/C2777938653","wikidata":"https://www.wikidata.org/wiki/Q1088156","display_name":"Chronic lymphocytic leukemia","level":3,"score":0.5695692896842957},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5639075636863708},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5146034955978394},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.49042245745658875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4391518235206604},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.35391873121261597},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.3206895887851715},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.263962984085083},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.21583348512649536},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10960754752159119}],"mesh":[{"descriptor_ui":"D003959","descriptor_name":"Diamines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007135","descriptor_name":"Immunoglobulin Variable Region","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007143","descriptor_name":"Immunoglobulin Heavy Chains","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009930","descriptor_name":"Organic Chemicals","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D009930","descriptor_name":"Organic Chemicals","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011804","descriptor_name":"Quinolines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012334","descriptor_name":"RNA, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012334","descriptor_name":"RNA, Neoplasm","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015326","descriptor_name":"Gene Rearrangement, B-Lymphocyte, Heavy Chain","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015451","descriptor_name":"Leukemia, Lymphocytic, Chronic, B-Cell","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D018365","descriptor_name":"Neoplasm, Residual","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D052160","descriptor_name":"Benzothiazoles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.2165/00066982-200509010-00004","doi":"https://doi.org/10.2165/00066982-200509010-00004","is_oa":false,"landing_page_url":"https://doi.org/10.2165/00066982-200509010-00004","pdf_url":null,"source":{"id":"https://openalex.org/S157311635","display_name":"Molecular Diagnosis","issn_l":"1084-8592","issn":["1084-8592","1532-8619"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core"],"host_organization":"https://openalex.org/P4310320330","host_organization_name":"Adis, Springer Healthcare","host_organization_lineage":["https://openalex.org/P4310320330","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Adis, Springer Healthcare","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Diagnosis","raw_type":"journal-article"},{"id":"pmid:16035732","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16035732","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9922999739646912,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W24911164","https://openalex.org/W60865865","https://openalex.org/W154103547","https://openalex.org/W1243376384","https://openalex.org/W1429374962","https://openalex.org/W1527161131","https://openalex.org/W1564208378","https://openalex.org/W1965851569","https://openalex.org/W1968444583","https://openalex.org/W1973916894","https://openalex.org/W1974774909","https://openalex.org/W1975274770","https://openalex.org/W1998582864","https://openalex.org/W2001686849","https://openalex.org/W2006099833","https://openalex.org/W2021646859","https://openalex.org/W2052415602","https://openalex.org/W2057569626","https://openalex.org/W2059980831","https://openalex.org/W2061847784","https://openalex.org/W2062803262","https://openalex.org/W2071056968","https://openalex.org/W2072441151","https://openalex.org/W2078673385","https://openalex.org/W2095807890","https://openalex.org/W2104235597","https://openalex.org/W2107794446","https://openalex.org/W2111609050","https://openalex.org/W2144634347","https://openalex.org/W2156804837","https://openalex.org/W2157119369","https://openalex.org/W2182988999","https://openalex.org/W2187693705","https://openalex.org/W2315265572","https://openalex.org/W2316778105","https://openalex.org/W2318933236","https://openalex.org/W2323490326","https://openalex.org/W2339083781","https://openalex.org/W2395711420","https://openalex.org/W2411887417","https://openalex.org/W2423351030"],"related_works":["https://openalex.org/W2588745578","https://openalex.org/W2566542046","https://openalex.org/W2986943437","https://openalex.org/W2023888678","https://openalex.org/W7099015853","https://openalex.org/W2072441151","https://openalex.org/W2159853837","https://openalex.org/W2559927347","https://openalex.org/W2075197983","https://openalex.org/W2171240476"],"abstract_inverted_index":null,"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}