{"id":"https://openalex.org/W2083476005","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","title":"Enzyme\u2010mediated peptide synthesis using acylpeptide hydrolase","display_name":"Enzyme\u2010mediated peptide synthesis using acylpeptide hydrolase","publication_year":1991,"publication_date":"1991-03-01","ids":{"openalex":"https://openalex.org/W2083476005","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","mag":"2083476005","pmid":"https://pubmed.ncbi.nlm.nih.gov/1688346"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1991.tb15866.x","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1991.tb15866.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1991.tb15866.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109038595","display_name":"Timothy C. FARRIES","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Timothy C. FARRIES","raw_affiliation_strings":["MRC Collaborative Centre, London, England"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"MRC Collaborative Centre, London, England","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111733224","display_name":"Anthony D. Auffret","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210093811","display_name":"Cambridge Science Centre","ror":"https://ror.org/00j7rye35","country_code":"GB","type":"archive","lineage":["https://openalex.org/I4210093811"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Anthony D. AUFFRET","raw_affiliation_strings":["Pharmacia LKB Biochrom Ltd, Cambridge, England"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacia LKB Biochrom Ltd, Cambridge, England","institution_ids":["https://openalex.org/I4210093811"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109333123","display_name":"Alastair Aitken","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405285715","display_name":"National Institute for Medical Research","ror":"https://ror.org/00146rb91","country_code":"GB","type":"facility","lineage":["https://openalex.org/I2801081054","https://openalex.org/I4210087105","https://openalex.org/I4405285715","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Alastair AITKEN","raw_affiliation_strings":["The National Institute for Medical Research, London, England"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The National Institute for Medical Research, London, England","institution_ids":["https://openalex.org/I4405285715"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5109333123"],"corresponding_institution_ids":["https://openalex.org/I4405285715"],"apc_list":null,"apc_paid":null,"fwci":1.3878,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.77170913,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"196","issue":"3","first_page":"687","last_page":"692"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.7124000191688538,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.7124000191688538,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.2793000042438507,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acylpeptide-hydrolase","display_name":"acylpeptide hydrolase","score":0.9919999837875366},{"id":"https://openalex.org/keywords/n-acetyl-amino-acids","display_name":"N-acetyl amino acids","score":0.8980000019073486},{"id":"https://openalex.org/keywords/dimethylformamide","display_name":"dimethylformamide","score":0.8949999809265137},{"id":"https://openalex.org/keywords/tripeptide-synthesis","display_name":"tripeptide synthesis","score":0.7860000133514404},{"id":"https://openalex.org/keywords/f-met-leu-phe","display_name":"fMet-Leu-Phe","score":0.9700000286102295},{"id":"https://openalex.org/keywords/superoxide-production","display_name":"superoxide production","score":0.8920000195503235},{"id":"https://openalex.org/keywords/granulocytes","display_name":"granulocytes","score":0.9259999990463257},{"id":"https://openalex.org/keywords/peptide-modification","display_name":"peptide modification","score":0.5649999976158142}],"concepts":[{"id":"https://openalex.org/C167392928","wikidata":"https://www.wikidata.org/wiki/Q4820777","display_name":"Tripeptide","level":3,"score":0.8215274810791016},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6863852143287659},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6515334844589233},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.641021728515625},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6329519748687744},{"id":"https://openalex.org/C2780120296","wikidata":"https://www.wikidata.org/wiki/Q96286","display_name":"Hydrolase","level":3,"score":0.6022855043411255},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.56800377368927},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.4619695544242859},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.4307026267051697},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.40240222215652466},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1991.tb15866.x","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1991.tb15866.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:1688346","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1688346","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/j.1432-1033.1991.tb15866.x","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1991.tb15866.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1991.tb15866.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2083476005.pdf"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W120904240","https://openalex.org/W1483714820","https://openalex.org/W1567668759","https://openalex.org/W1854512591","https://openalex.org/W1972344351","https://openalex.org/W2096176556","https://openalex.org/W2109503201","https://openalex.org/W2119221986","https://openalex.org/W2125516765","https://openalex.org/W2127682808","https://openalex.org/W2216893531","https://openalex.org/W2320556745","https://openalex.org/W2330184825","https://openalex.org/W4233037567","https://openalex.org/W4233287987","https://openalex.org/W4254811673","https://openalex.org/W4295981328","https://openalex.org/W4297959863"],"related_works":["https://openalex.org/W2093386317","https://openalex.org/W2333293300","https://openalex.org/W2127682808","https://openalex.org/W2317272685","https://openalex.org/W4234838866","https://openalex.org/W2467451419","https://openalex.org/W1592218319","https://openalex.org/W1992597914","https://openalex.org/W2159085559","https://openalex.org/W2041893317"],"abstract_inverted_index":{"Acylpeptide":[0,110],"hydrolase":[1,111],"is":[2,27,45],"shown":[3],"to":[4,14,29,38],"catalyse":[5],"the":[6,15,25,51,54,120],"specific":[7],"addition":[8],"of":[9,17,24,50,56,60,76,124],"a":[10,18,31,39,115],"single":[11],"amino":[12,36,87],"acid":[13,37,88],"N-terminus":[16],"peptide.":[19,42],"The":[20,43,81,95],"stabilised":[21],"Sepharose-coupled":[22],"form":[23],"enzyme":[26],"used":[28],"couple":[30],"carboxy-methylated":[32],"N-formyl":[33],"(or":[34],"N-acetyl)":[35],"short":[40],"pre-existing":[41],"yield":[44],"improved":[46],"by":[47,71,85,108],"optimal":[48],"timing":[49],"reaction":[52],"and":[53,65,78,92,122],"presence":[55],"moderate":[57],"concentrations":[58],"(5%)":[59],"N,N-dimethylformamide.":[61],"Two":[62],"tripeptides,":[63],"Ac-Ala-Ala-Ala":[64],"fMet-Leu-Phe":[66,97],"(f,":[67],"formyl)":[68],"were":[69,83],"synthesized":[70],"this":[72],"technique":[73],"(in":[74],"yields":[75],"2%":[77],"0.064%":[79],"respectively).":[80],"products":[82],"characterised":[84],"HPLC,":[86],"analysis,":[89],"mass":[90],"spectroscopy":[91],"protein":[93],"sequencing.":[94],"synthetic":[96],"also":[98],"had":[99],"biological":[100],"activity,":[101],"in":[102],"that":[103],"it":[104],"stimulated":[105],"superoxide":[106],"generation":[107],"granulocytes.":[109],"could":[112],"therefore":[113],"be":[114],"very":[116],"useful":[117],"tool":[118],"for":[119],"synthesis":[121],"modification":[123],"peptides.":[125]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}