{"id":"https://openalex.org/W2083040197","doi":"https://doi.org/10.1021/ac035275m","title":"Use of Polymer-Modified MALDI-MS Probes To Improve Analyses of Protein Digests and DNA","display_name":"Use of Polymer-Modified MALDI-MS Probes To Improve Analyses of Protein Digests and DNA","publication_year":2004,"publication_date":"2004-05-01","ids":{"openalex":"https://openalex.org/W2083040197","doi":"https://doi.org/10.1021/ac035275m","mag":"2083040197","pmid":"https://pubmed.ncbi.nlm.nih.gov/15167789"},"language":"en","primary_location":{"id":"doi:10.1021/ac035275m","doi":"https://doi.org/10.1021/ac035275m","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac035275m","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101931121","display_name":"Yingda Xu","orcid":"https://orcid.org/0000-0003-0637-177X","observed_orcids":["https://orcid.org/0000-0003-0637-177X"]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yingda Xu","raw_affiliation_strings":["Department of Chemistry, Michigan State University, East Lansing, Michigan 48824","Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Michigan State University, East Lansing, Michigan 48824","institution_ids":["https://openalex.org/I87216513"]},{"raw_affiliation_string":"Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025107183","display_name":"Merlin L. Bruening","orcid":"https://orcid.org/0000-0002-4553-5143","observed_orcids":["https://orcid.org/0000-0002-4553-5143"]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Merlin L. Bruening","raw_affiliation_strings":["Department of Chemistry, Michigan State University, East Lansing, Michigan 48824"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Michigan State University, East Lansing, Michigan 48824","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109856555","display_name":"Jack Throck Watson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Throck Watson","raw_affiliation_strings":["Department of Chemistry, Michigan State University, East Lansing, Michigan 48824"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Michigan State University, East Lansing, Michigan 48824","institution_ids":["https://openalex.org/I87216513"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I87216513"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.593,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.81371259,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"76","issue":"11","first_page":"3106","last_page":"3111"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9746000170707703,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9746000170707703,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.02419999986886978,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12166","display_name":"Ion-surface interactions and analysis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2206","display_name":"Computational Mechanics"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/surface-patterning","display_name":"surface patterning","score":0.5389999747276306},{"id":"https://openalex.org/keywords/selective-adsorption","display_name":"selective adsorption","score":0.7239999771118164},{"id":"https://openalex.org/keywords/proteolytic-peptides","display_name":"proteolytic peptides","score":0.7419999837875366},{"id":"https://openalex.org/keywords/myoglobin","display_name":"myoglobin","score":0.7860000133514404},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/phosphoproteomics","display_name":"phosphoproteomics","score":0.5889999866485596},{"id":"https://openalex.org/keywords/phosphopeptides","display_name":"phosphopeptides","score":0.9120000004768372},{"id":"https://openalex.org/keywords/polyethylenimine","display_name":"polyethyleneimine","score":0.9449999928474426},{"id":"https://openalex.org/keywords/ionization-efficiency","display_name":"ionization efficiency","score":0.6110000014305115}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.984603762626648},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6031612157821655},{"id":"https://openalex.org/C75280812","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Matrix-assisted laser desorption/ionization","level":4,"score":0.5417895317077637},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.48486170172691345},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4621489942073822},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2511778473854065},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1162518858909607},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.0},{"id":"https://openalex.org/C162711632","wikidata":"https://www.wikidata.org/wiki/Q905514","display_name":"Desorption","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000180","descriptor_name":"Acrylic Resins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011094","descriptor_name":"Polyethyleneimine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011094","descriptor_name":"Polyethyleneimine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D015335","descriptor_name":"Molecular Probes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ac035275m","doi":"https://doi.org/10.1021/ac035275m","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac035275m","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:15167789","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15167789","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","score":0.6800000071525574,"display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5662183989","display_name":"DISULFIDE STRUCTURE OF BIOMEDICALLY IMPORTANT PROTEINS","funder_award_id":"R01GM060576","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320310016","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W113188583","https://openalex.org/W1980500623","https://openalex.org/W1981511045","https://openalex.org/W1996693787","https://openalex.org/W2004207584","https://openalex.org/W2021236905","https://openalex.org/W2030157681","https://openalex.org/W2034044070","https://openalex.org/W2037186777","https://openalex.org/W2046461084","https://openalex.org/W2047799344","https://openalex.org/W2048280438","https://openalex.org/W2050484612","https://openalex.org/W2057509544","https://openalex.org/W2058643233","https://openalex.org/W2059469231","https://openalex.org/W2062567078","https://openalex.org/W2065934073","https://openalex.org/W2069433087","https://openalex.org/W2070410037","https://openalex.org/W2074645475","https://openalex.org/W2077370394","https://openalex.org/W2087221126","https://openalex.org/W2088224730","https://openalex.org/W2102518406","https://openalex.org/W2125386813","https://openalex.org/W2140121506","https://openalex.org/W2165923048","https://openalex.org/W2322162638","https://openalex.org/W2329729197","https://openalex.org/W2399251643","https://openalex.org/W4253076341"],"related_works":["https://openalex.org/W18700180","https://openalex.org/W2009701626","https://openalex.org/W2047799344","https://openalex.org/W1997550620","https://openalex.org/W2070410037","https://openalex.org/W2313339293","https://openalex.org/W6945329245","https://openalex.org/W1966635981","https://openalex.org/W2078002067","https://openalex.org/W2003222890"],"abstract_inverted_index":{"The":[0,162,176],"use":[1,95,177],"of":[2,10,18,33,40,49,67,89,96,111,120,178,191,203],"sample":[3,43,59,78,101,148],"probe":[4,60,79,149],"surfaces":[5,30,181],"patterned":[6],"with":[7,62,160],"200-microm-diameter":[8],"spots":[9],"hydrophilic,":[11],"charged":[12],"polymers":[13],"significantly":[14],"enhances":[15],"the":[16,41,47,53,87,94,138,168,173,201],"analysis":[17,72,88,212],"protein":[19],"digests":[20,122],"and":[21,189],"DNA":[22,192],"by":[23,123,152,213],"MALDI-MS.":[24,214],"Selective":[25],"adsorption":[26,188],"on":[27],"these":[28,126],"polymer-modified":[29],"allows":[31],"collection":[32],"specific":[34],"proteolytic":[35,69,128,140],"peptides,":[36],"while":[37,71],"subsequent":[38,211],"rinsing":[39],"deposited":[42],"removes":[44],"contaminants.":[45],"In":[46],"case":[48],"partially":[50],"digested":[51],"myoglobin,":[52],"mass":[54,208],"spectrum":[55],"obtained":[56],"using":[57,73,146],"a":[58,74,147,153,194],"modified":[61,151],"polyanionic":[63],"functionalities":[64],"permits":[65],"detection":[66],"22":[68],"fragments,":[70],"stainless":[75],"steel":[76],"MALDI":[77,100,135],"gives":[80],"only":[81],"11":[82],"detectable":[83],"fragments.":[84],"Similarly,":[85],"during":[86,118,210],"bovine":[90],"serum":[91],"albumin":[92],"digests,":[93],"several":[97],"different":[98],"surface-modified":[99],"probes":[102],"increases":[103],"sequence":[104],"coverage":[105],"from":[106,137],"61.3":[107],"to":[108],"74.5%.":[109],"Detection":[110],"phosphorylated":[112,139,174],"peptides":[113],"can":[114],"be":[115],"quite":[116],"challenging":[117],"analyses":[119],"phosphoprotein":[121],"MALDI-MS":[124],"because":[125,167],"anionic":[127],"fragments":[129,141],"have":[130],"low":[131],"ionization":[132],"efficiencies.":[133],"However,":[134],"signals":[136],"sometimes":[142],"increase":[143],"dramatically":[144],"when":[145],"surface":[150,170],"polycation":[154],"(polyethylenimine":[155],"or":[156,199],"poly(acrylic":[157],"acid)":[158],"complexed":[159],"Fe(3+)).":[161],"signal":[163],"enhancement":[164],"apparently":[165],"occurs":[166],"positive":[169],"selectively":[171],"binds":[172],"peptides.":[175],"patterned,":[179],"polycationic":[180],"also":[182],"shows":[183],"great":[184],"promise":[185],"for":[186],"selective":[187],"decontamination":[190],"samples;":[193],"simple":[195],"water":[196],"rinse":[197],"diminishes":[198],"eliminates":[200],"formation":[202],"multi-ion":[204],"adducts,":[205],"thereby":[206],"improving":[207],"resolution":[209]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}