{"id":"https://openalex.org/W2082979206","doi":"https://doi.org/10.1117/12.485720","title":"Monitoring of DsRed protein concentration in frozen insect larvae","display_name":"Monitoring of DsRed protein concentration in frozen insect larvae","publication_year":2003,"publication_date":"2003-07-30","ids":{"openalex":"https://openalex.org/W2082979206","doi":"https://doi.org/10.1117/12.485720","mag":"2082979206"},"language":"en","primary_location":{"id":"doi:10.1117/12.485720","doi":"https://doi.org/10.1117/12.485720","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.485720","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046823574","display_name":"Yordan V. Kostov","orcid":"https://orcid.org/0000-0002-7903-9682","observed_orcids":["https://orcid.org/0000-0002-7903-9682"]},"institutions":[{"id":"https://openalex.org/I79272384","display_name":"University of Maryland, Baltimore County","ror":"https://ror.org/02qskvh78","country_code":"US","type":"education","lineage":["https://openalex.org/I79272384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yordan Kostov","raw_affiliation_strings":["Univ. of Maryland, Baltimore County (United States)","University of Maryland, Baltimore County, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ. of Maryland, Baltimore County (United States)","institution_ids":["https://openalex.org/I79272384"]},{"raw_affiliation_string":"University of Maryland, Baltimore County, United States","institution_ids":["https://openalex.org/I79272384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043940183","display_name":"Leah M. Tolosa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79272384","display_name":"University of Maryland, Baltimore County","ror":"https://ror.org/02qskvh78","country_code":"US","type":"education","lineage":["https://openalex.org/I79272384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Leah Tolosa","raw_affiliation_strings":["Univ. of Maryland, Baltimore County (United States)","University of Maryland, Baltimore County, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ. of Maryland, Baltimore County (United States)","institution_ids":["https://openalex.org/I79272384"]},{"raw_affiliation_string":"University of Maryland, Baltimore County, United States","institution_ids":["https://openalex.org/I79272384"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kevin O'Connell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304082316","display_name":"United States Department of the Army","ror":"https://ror.org/035w1gb98","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796"]},{"id":"https://openalex.org/I4210088792","display_name":"United States Army","ror":"https://ror.org/00afsp483","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796","https://openalex.org/I4210088792"]},{"id":"https://openalex.org/I4387153805","display_name":"U.S. Army Combat Capabilities Development Command Chemical Biological Center","ror":"https://ror.org/022j0mn33","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796","https://openalex.org/I2802705668","https://openalex.org/I4210154437","https://openalex.org/I4387153805"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin O'Connell","raw_affiliation_strings":["U.S. Army Edgewood Chemical Biological Ctr. (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Army Edgewood Chemical Biological Ctr. (United States)","institution_ids":["https://openalex.org/I1304082316","https://openalex.org/I4210088792","https://openalex.org/I4387153805"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011304859","display_name":"Patricia E. D. Anderson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304082316","display_name":"United States Department of the Army","ror":"https://ror.org/035w1gb98","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796"]},{"id":"https://openalex.org/I4210088792","display_name":"United States Army","ror":"https://ror.org/00afsp483","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796","https://openalex.org/I4210088792"]},{"id":"https://openalex.org/I4387153805","display_name":"U.S. Army Combat Capabilities Development Command Chemical Biological Center","ror":"https://ror.org/022j0mn33","country_code":"US","type":"government","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1330347796","https://openalex.org/I2802705668","https://openalex.org/I4210154437","https://openalex.org/I4387153805"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patricia Anderson","raw_affiliation_strings":["U.S. Army Edgewood Chemical Biological Ctr. (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Army Edgewood Chemical Biological Ctr. (United States)","institution_ids":["https://openalex.org/I1304082316","https://openalex.org/I4210088792","https://openalex.org/I4387153805"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100330523","display_name":"Yi Liu","orcid":"https://orcid.org/0000-0002-3954-6102","observed_orcids":["https://orcid.org/0000-0002-3954-6102","https://orcid.org/0000-0002-5821-9086","https://orcid.org/0009-0004-7493-6183","https://orcid.org/0009-0003-2974-9234","https://orcid.org/0009-0000-4340-6463","https://orcid.org/0000-0002-7114-7080"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yi Liu","raw_affiliation_strings":["Chesapeak PERL, Inc. (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chesapeak PERL, Inc. (United States)","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033202251","display_name":"Nikolai van Beek","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nikolai van Beek","raw_affiliation_strings":["Chesapeak PERL, Inc. (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chesapeak PERL, Inc. (United States)","institution_ids":[]}]},{"author_position":"last","author":{"id":null,"display_name":"Govind Rao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79272384","display_name":"University of Maryland, Baltimore County","ror":"https://ror.org/02qskvh78","country_code":"US","type":"education","lineage":["https://openalex.org/I79272384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Govind Rao","raw_affiliation_strings":["Univ. of Maryland, Baltimore County (United States)","University of Maryland, Baltimore County, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ. of Maryland, Baltimore County (United States)","institution_ids":["https://openalex.org/I79272384"]},{"raw_affiliation_string":"University of Maryland, Baltimore County, United States","institution_ids":["https://openalex.org/I79272384"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.14001049,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"4967","issue":null,"first_page":"100","last_page":"100"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.5752000212669373,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.5752000212669373,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.1445000022649765,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.07419999688863754,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.8970000147819519},{"id":"https://openalex.org/keywords/insect-larvae","display_name":"insect larvae","score":0.8700000047683716},{"id":"https://openalex.org/keywords/optical-probe","display_name":"optical probe","score":0.7129999995231628},{"id":"https://openalex.org/keywords/fluorescence-detection","display_name":"fluorescence detection","score":0.5410000085830688},{"id":"https://openalex.org/keywords/probe-calibration","display_name":"probe calibration","score":0.5989999771118164},{"id":"https://openalex.org/keywords/harvest-timing","display_name":"harvest time","score":0.4129999876022339}],"concepts":[{"id":"https://openalex.org/C202800777","wikidata":"https://www.wikidata.org/wiki/Q1781297","display_name":"Fluorometer","level":3,"score":0.8361523151397705},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.5915543437004089},{"id":"https://openalex.org/C2777612826","wikidata":"https://www.wikidata.org/wiki/Q1390","display_name":"Insect","level":2,"score":0.5337473154067993},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.48800745606422424},{"id":"https://openalex.org/C6856738","wikidata":"https://www.wikidata.org/wiki/Q331672","display_name":"Protein expression","level":3,"score":0.4770740270614624},{"id":"https://openalex.org/C23531484","wikidata":"https://www.wikidata.org/wiki/Q3196863","display_name":"Calibration curve","level":3,"score":0.45280858874320984},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3869365155696869},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.35080379247665405},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33000630140304565},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.31269150972366333},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.22187760472297668},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.1649377942085266},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.13527444005012512},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12379533052444458},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.10331827402114868},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0956980288028717},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0762968361377716},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1117/12.485720","doi":"https://doi.org/10.1117/12.485720","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.485720","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2009103186","https://openalex.org/W2040126189","https://openalex.org/W2016830886","https://openalex.org/W1979120533","https://openalex.org/W2062688623","https://openalex.org/W163254623","https://openalex.org/W2079865838","https://openalex.org/W4416751436","https://openalex.org/W2002496467","https://openalex.org/W2091270150"],"abstract_inverted_index":{"A":[0],"baculovirus":[1],"expression":[2],"system":[3,18],"was":[4,36,65],"used":[5,37],"to":[6,23],"produce":[7],"DsRed":[8],"fusion":[9],"protein":[10,42],"in":[11,44,71],"insect":[12],"larvae.":[13,47],"As":[14],"the":[15,41,45,63,85,89],"baculovirus/insect":[16],"larvae":[17,52],"requires":[19],"precise":[20],"harvest":[21],"timing":[22],"achieve":[24],"high":[25],"yield":[26],"of":[27,40,50,62],"protein,":[28],"a":[29],"low-cost":[30],"miniature":[31],"all-solid":[32],"state":[33],"optical":[34,86],"probe":[35,64],"for":[38],"detection":[39],"concentrations":[43],"frozen":[46],"Three":[48],"batches":[49],"infected":[51],"were":[53],"monitored":[54],"at":[55],"different":[56],"post-infection":[57],"times.":[58],"The":[59,79],"calibration":[60],"curve":[61],"obtained":[66],"by":[67],"simultaneous":[68],"measurements":[69,87],"both":[70],"laboratory":[72,91],"fluorimeter":[73],"and":[74,88],"using":[75],"gel":[76],"electrophoresis":[77],"analysis.":[78],"results":[80],"show":[81],"good":[82],"correlation":[83],"between":[84],"standard":[90],"technique.":[92]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}