{"id":"https://openalex.org/W2082849164","doi":"https://doi.org/10.1021/bi00152a017","title":"Calorimetric study of the heat and cold denaturation of .beta.-lactoglobulin","display_name":"Calorimetric study of the heat and cold denaturation of .beta.-lactoglobulin","publication_year":1992,"publication_date":"1992-09-22","ids":{"openalex":"https://openalex.org/W2082849164","doi":"https://doi.org/10.1021/bi00152a017","mag":"2082849164","pmid":"https://pubmed.ncbi.nlm.nih.gov/1390668"},"language":"en","primary_location":{"id":"doi:10.1021/bi00152a017","doi":"https://doi.org/10.1021/bi00152a017","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00152a017","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111869758","display_name":"Yu.V. Griko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Yu. V. Griko","raw_affiliation_strings":["Institute of Protein Research, Russian Academy of Sciences, Puschino"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Russian Academy of Sciences, Puschino","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210137602"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112756627","display_name":"Peter L. Privalov","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P. L. Privalov","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.7879,"has_fulltext":false,"cited_by_count":118,"citation_normalized_percentile":{"value":0.85723102,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"31","issue":"37","first_page":"8810","last_page":"8815"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.09070000052452087,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.017799999564886093,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-lactoglobulin","display_name":"\u03b2-lactoglobulin","score":0.9929999709129333},{"id":"https://openalex.org/keywords/cold-denaturation","display_name":"cold denaturation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/thermal-denaturation","display_name":"thermal denaturation","score":0.996999979019165},{"id":"https://openalex.org/keywords/urea-denaturation","display_name":"urea denaturation","score":0.7630000114440918},{"id":"https://openalex.org/keywords/two-state-transition","display_name":"two-state transition","score":0.7390000224113464},{"id":"https://openalex.org/keywords/protein-unfolding","display_name":"protein unfolding","score":0.6389999985694885},{"id":"https://openalex.org/keywords/denatured-proteins","display_name":"denatured proteins","score":0.6159999966621399},{"id":"https://openalex.org/keywords/denaturation-enthalpy","display_name":"denaturation enthalpy","score":0.753000020980835},{"id":"https://openalex.org/keywords/protein-thermodynamics","display_name":"protein thermodynamics","score":0.38199999928474426}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.7621954679489136},{"id":"https://openalex.org/C2778447006","wikidata":"https://www.wikidata.org/wiki/Q1156474","display_name":"Icon","level":2,"score":0.663323163986206},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5322997570037842},{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.4940510094165802},{"id":"https://openalex.org/C2778407487","wikidata":"https://www.wikidata.org/wiki/Q14565201","display_name":"Altmetrics","level":2,"score":0.46954473853111267},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.4605947732925415},{"id":"https://openalex.org/C3019144022","wikidata":"https://www.wikidata.org/wiki/Q4124998","display_name":"Questions and answers","level":2,"score":0.42171066999435425},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.4130687713623047},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.34988343715667725},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.2506270110607147},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002151","descriptor_name":"Calorimetry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003080","descriptor_name":"Cold Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007782","descriptor_name":"Lactoglobulins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014508","descriptor_name":"Urea","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00152a017","doi":"https://doi.org/10.1021/bi00152a017","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00152a017","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:1390668","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1390668","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W28039676","https://openalex.org/W187801720","https://openalex.org/W1496368635","https://openalex.org/W1524706855","https://openalex.org/W1569015250","https://openalex.org/W1971112995","https://openalex.org/W1982224344","https://openalex.org/W1988472868","https://openalex.org/W2003713109","https://openalex.org/W2004421107","https://openalex.org/W2011005442","https://openalex.org/W2013414174","https://openalex.org/W2021922906","https://openalex.org/W2025240528","https://openalex.org/W2038634233","https://openalex.org/W2054342921","https://openalex.org/W2055594423","https://openalex.org/W2057246411","https://openalex.org/W2074947829","https://openalex.org/W2076529933","https://openalex.org/W2100837269","https://openalex.org/W2403923750","https://openalex.org/W2404479285"],"related_works":["https://openalex.org/W2395828917","https://openalex.org/W2011770880","https://openalex.org/W3143695208","https://openalex.org/W2060005665","https://openalex.org/W1997417998","https://openalex.org/W2008089333","https://openalex.org/W2064698049","https://openalex.org/W2058489385","https://openalex.org/W2004421107","https://openalex.org/W4235162515"],"abstract_inverted_index":{"Temperature-induced":[0],"changes":[1],"of":[2,5,14,18,62,71,92,95,101,112,120,125,133,149,156,191,218,232],"the":[3,12,42,48,56,87,98,102,109,117,129,134,138,145,152,157,168,188,192,201,216,219,226,230,237],"states":[4],"beta-lactoglobulin":[6,220],"have":[7],"been":[8],"studied":[9],"calorimetrically.":[10],"In":[11],"presence":[13,231],"a":[15,38],"high":[16],"concentration":[17],"urea":[19,96,126,150,176],"this":[20,52],"protein":[21,103,159,194,239],"shows":[22],"not":[23,214,241],"only":[24,178,246],"heat":[25,31,60,72,90,113,131,146,154,189],"but":[26,177],"also":[27],"cold":[28,43,63,121],"denaturation.":[29,122],"Its":[30],"denaturation":[32,44,64,73,114],"is":[33,65,75,84,160,195,223,240],"approximated":[34],"very":[35],"closely":[36],"by":[37,86],"two-state":[39,49],"transition,":[40],"while":[41],"deviates":[45],"considerably":[46,127],"from":[47],"transition":[50],"and":[51,74,115,244],"deviation":[53],"increases":[54,116,128],"as":[55],"temperature":[57],"decreases.":[58],"The":[59,123],"effect":[61,91,148],"opposite":[66],"in":[67,78,82,137,162,172,229],"sign":[68],"to":[69,97,164,225],"that":[70,165,198,207],"noticeably":[76],"larger":[77],"magnitude.":[79],"This":[80],"difference":[81],"magnitude":[83,163],"caused":[85],"temperature-dependent":[88],"negative":[89,118],"additional":[93],"binding":[94,124],"polypeptide":[99,170,203,221],"chain":[100,171,222],"upon":[104],"its":[105],"unfolding,":[106],"which":[107],"decreases":[108],"positive":[110],"enthalpy":[111,119],"partial":[130,153],"capacity":[132,147,155,190],"protein,":[135],"especially":[136],"denatured":[139,158,193,238],"state.":[140],"However,":[141],"when":[142],"corrected":[143],"for":[144,167,179,200],"binding,":[151],"close":[161],"expected":[166,199],"unfolded":[169,202,243],"aqueous":[173],"solution":[174],"without":[175],"temperatures":[180,209,248],"below":[181],"10":[182,211,251],"degrees":[183,212,252],"C.":[184],"At":[185],"higher":[186],"temperatures,":[187],"lower":[196,249],"than":[197,250],"chain.":[204],"It":[205],"appears":[206],"at":[208,247],"above":[210],"C":[213],"all":[215],"surface":[217],"exposed":[224],"solvent,":[227],"even":[228],"6":[233],"M":[234],"urea;":[235],"i.e.,":[236],"completely":[242],"unfolds":[245],"C.(ABSTRACT":[253],"TRUNCATED":[254],"AT":[255],"250":[256],"WORDS)":[257]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}