{"id":"https://openalex.org/W2082513628","doi":"https://doi.org/10.1159/000017982","title":"Ki-67 Immunostaining of Normal Human Epidermis: Comparison with 3H-Thymidine Labelling and PCNA Immunostaining","display_name":"Ki-67 Immunostaining of Normal Human Epidermis: Comparison with 3H-Thymidine Labelling and PCNA Immunostaining","publication_year":1998,"publication_date":"1998-01-01","ids":{"openalex":"https://openalex.org/W2082513628","doi":"https://doi.org/10.1159/000017982","mag":"2082513628","pmid":"https://pubmed.ncbi.nlm.nih.gov/9732159"},"language":"en","primary_location":{"id":"doi:10.1159/000017982","doi":"https://doi.org/10.1159/000017982","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000017982","pdf_url":null,"source":{"id":"https://openalex.org/S135397728","display_name":"Dermatology","issn_l":"1018-8665","issn":["1018-8665","1421-9832"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Dermatology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109181727","display_name":"M. Heenen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2802054198","display_name":"Erasmus Hospital","ror":"https://ror.org/05j1gs298","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I2802054198"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"M. Heenen","raw_affiliation_strings":["Department of Dermatology, H\u00f4pital Acad\u00e9mique Erasme, Bruxelles, Belgium","Dermatology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Dermatology, H\u00f4pital Acad\u00e9mique Erasme, Bruxelles, Belgium","institution_ids":["https://openalex.org/I2802054198"]},{"raw_affiliation_string":"Dermatology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002699725","display_name":"S. Thiriar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S. Thiriar","raw_affiliation_strings":["Dermatology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dermatology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111247479","display_name":"J.C. No\u00ebl","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2802054198","display_name":"Erasmus Hospital","ror":"https://ror.org/05j1gs298","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I2802054198"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"J.-C. No\u00ebl","raw_affiliation_strings":["Anatomopathology, H\u00f4pital Acad\u00e9mique Erasme, Bruxelles, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Anatomopathology, H\u00f4pital Acad\u00e9mique Erasme, Bruxelles, and","institution_ids":["https://openalex.org/I2802054198"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023153305","display_name":"Paul Galand","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I132053463","display_name":"Universit\u00e9 Libre de Bruxelles","ror":"https://ror.org/01r9htc13","country_code":"BE","type":"education","lineage":["https://openalex.org/I132053463"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"P. Galand","raw_affiliation_strings":["Laboratory of Cytology and Experimental Cancerology, School of Medicine, Universit\u00e9 Libre de Bruxelles, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Cytology and Experimental Cancerology, School of Medicine, Universit\u00e9 Libre de Bruxelles, Belgium","institution_ids":["https://openalex.org/I132053463"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4852,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.54746065,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"197","issue":"2","first_page":"123","last_page":"126"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11167","display_name":"Hair Growth and Disorders","score":0.0215000007301569,"subfield":{"id":"https://openalex.org/subfields/2708","display_name":"Dermatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11306","display_name":"Nonmelanoma Skin Cancer Studies","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2708","display_name":"Dermatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ki-67","display_name":"Ki-67","score":0.9729999899864197},{"id":"https://openalex.org/keywords/pcna","display_name":"PCNA","score":0.7739999890327454},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.6380000114440918},{"id":"https://openalex.org/keywords/3h-thymidine-labeling-index","display_name":"3H-thymidine labeling index","score":0.8830000162124634},{"id":"https://openalex.org/keywords/s-phase","display_name":"S phase","score":0.7570000290870667},{"id":"https://openalex.org/keywords/antigen-retrieval","display_name":"antigen retrieval","score":0.6150000095367432},{"id":"https://openalex.org/keywords/microwave-processing","display_name":"microwave processing","score":0.5809999704360962},{"id":"https://openalex.org/keywords/basal-cells","display_name":"basal cells","score":0.7400000095367432},{"id":"https://openalex.org/keywords/cell-cycle","display_name":"cell cycle","score":0.9100000262260437}],"concepts":[{"id":"https://openalex.org/C4224716","wikidata":"https://www.wikidata.org/wiki/Q10859258","display_name":"Immunostaining","level":3,"score":0.9567171335220337},{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.6517921686172485},{"id":"https://openalex.org/C2776458125","wikidata":"https://www.wikidata.org/wiki/Q1827343","display_name":"Epidermis (zoology)","level":2,"score":0.6319029331207275},{"id":"https://openalex.org/C2780523633","wikidata":"https://www.wikidata.org/wiki/Q380709","display_name":"Labelling","level":2,"score":0.6050764322280884},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5449792146682739},{"id":"https://openalex.org/C34688535","wikidata":"https://www.wikidata.org/wiki/Q418732","display_name":"Proliferating cell nuclear antigen","level":3,"score":0.5163918137550354},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.483224481344223},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4693140983581543},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39967983961105347},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36376476287841797},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.23390230536460876},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.19323062896728516},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.14414256811141968},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11360156536102295}],"mesh":[{"descriptor_ui":"D000078404","descriptor_name":"Epidermal Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D004817","descriptor_name":"Epidermis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008872","descriptor_name":"Microwaves","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008940","descriptor_name":"Mitotic Index","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016196","descriptor_name":"S Phase","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018809","descriptor_name":"Proliferating Cell Nuclear Antigen","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D019394","descriptor_name":"Ki-67 Antigen","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1159/000017982","doi":"https://doi.org/10.1159/000017982","is_oa":false,"landing_page_url":"https://doi.org/10.1159/000017982","pdf_url":null,"source":{"id":"https://openalex.org/S135397728","display_name":"Dermatology","issn_l":"1018-8665","issn":["1018-8665","1421-9832"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310317820","host_organization_name":"Karger Publishers","host_organization_lineage":["https://openalex.org/P4310317820"],"host_organization_lineage_names":["Karger Publishers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Dermatology","raw_type":"journal-article"},{"id":"pmid:9732159","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9732159","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Dermatology (Basel, Switzerland)","raw_type":"Comparative Study"},{"id":"pmh:oai:dipot.ulb.ac.be:2013/92311","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/92311","pdf_url":null,"source":{"id":"https://openalex.org/S4306401063","display_name":"D\u00e9p\u00f4t institutionnel de l'Universit\u00e9 libre de Bruxelles (Universit\u00e9 Libre de Bruxelles)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I132053463","host_organization_name":"Universit\u00e9 Libre de Bruxelles","host_organization_lineage":["https://openalex.org/I132053463"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Dermatology, 197 (2","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W123651699","https://openalex.org/W651268857","https://openalex.org/W1967852110","https://openalex.org/W1967880575","https://openalex.org/W1971015124","https://openalex.org/W1974012433","https://openalex.org/W1984786738","https://openalex.org/W1994346966","https://openalex.org/W2019476728","https://openalex.org/W2023321396","https://openalex.org/W2025354892","https://openalex.org/W2029504959","https://openalex.org/W2031504305","https://openalex.org/W2039810749","https://openalex.org/W2057100587","https://openalex.org/W2061932883","https://openalex.org/W2080154945","https://openalex.org/W2087178337","https://openalex.org/W2126486896","https://openalex.org/W2137781978","https://openalex.org/W2155643713","https://openalex.org/W2473890412"],"related_works":["https://openalex.org/W2055467999","https://openalex.org/W2072448326","https://openalex.org/W2328846709","https://openalex.org/W2091368954","https://openalex.org/W2073875483","https://openalex.org/W2432610241","https://openalex.org/W2002688691","https://openalex.org/W2130126671","https://openalex.org/W1527721103","https://openalex.org/W2058538432"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,128,134],"size":[2],"of":[3,11,16,24,33,39,71,94,112,136,176,181,193],"the":[4,9,40,50,92,113,149,152,173,177],"germinative":[5,14,178],"growth":[6,41,174],"fraction":[7,42,175],"(i.e.":[8],"number":[10],"actively":[12],"proliferating":[13],"cells)":[15],"normal":[17,72,182],"human":[18,73,183],"epidermis":[19,74,184],"is":[20,185,191],"still":[21],"a":[22,196],"subject":[23],"debate.":[25],"Ki-67":[26,56],"antigen":[27,81,143],"and":[28,79,121,190],"PCNA,":[29],"an":[30],"auxiliary":[31],"protein":[32],"d-polymerase,":[34],"are":[35],"considered":[36],"as":[37,91],"markers":[38],"when":[43],"used":[44],"under":[45],"optimal":[46],"conditions.":[47],"METHOD:":[48],"In":[49,148,159],"present":[51],"work,":[52],"we":[53],"have":[54],"compared":[55],"expression":[57,64],"(detected":[58,65],"with":[59,62,66,195],"MIB1":[60,119],"antibody)":[61,68],"PCNA":[63],"PC10":[67,126,161],"in":[69,76,96,103,164],"biopsies":[70,99],"fixed":[75],"neutral":[77],"formalin":[78],"using":[80],"retrieval":[82,144],"by":[83,145],"microwave":[84,109,146],"processing.":[85,147],"To":[86],"obtain":[87],"additional":[88],"information,":[89],"such":[90],"percentage":[93],"cells":[95,115,138,194],"S":[97],"phase,":[98],"were":[100,116,123],"also":[101],"incubated":[102],"3H-thymidine":[104,129],"before":[105],"immunostaining.":[106],"RESULTS:":[107],"Before":[108],"treatment,":[110],"8%":[111],"basal":[114],"positive":[117,124,163],"for":[118,125],"antibody":[120],"7.8%":[122],"antibody.":[127],"labelling":[130,154],"index":[131,155],"was":[132],"2.8%.":[133],"proportion":[135],"MIB1-positive":[137],"rose":[139,156],"to":[140,157],"19%":[141],"after":[142],"same":[150],"way,":[151],"3H":[153],"9%.":[158],"contrast,":[160],"became":[162],"all":[165],"epidermal":[166],"nuclei.":[167],"CONCLUSION:":[168],"These":[169],"results":[170],"suggest":[171],"that":[172],"cell":[179,198],"population":[180],"not":[186],"larger":[187],"than":[188],"20%":[189],"composed":[192],"short":[197],"cycle":[199],"time.":[200]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}