{"id":"https://openalex.org/W2081817690","doi":"https://doi.org/10.1007/bf02537503","title":"Cholesterol and 25\u2010hydroxycholesterol retention in specimens of liver and aorta prepared for electron microscopy. II. Effect of filipin, osmium, digitonin and saponin","display_name":"Cholesterol and 25\u2010hydroxycholesterol retention in specimens of liver and aorta prepared for electron microscopy. II. Effect of filipin, osmium, digitonin and saponin","publication_year":1993,"publication_date":"1993-10-01","ids":{"openalex":"https://openalex.org/W2081817690","doi":"https://doi.org/10.1007/bf02537503","mag":"2081817690","pmid":"https://pubmed.ncbi.nlm.nih.gov/8246694"},"language":"en","primary_location":{"id":"doi:10.1007/bf02537503","doi":"https://doi.org/10.1007/bf02537503","is_oa":false,"landing_page_url":"https://doi.org/10.1007/bf02537503","pdf_url":null,"source":{"id":"https://openalex.org/S115623499","display_name":"Lipids","issn_l":"0024-4201","issn":["0024-4201","1558-9307"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lipids","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082988494","display_name":"E. Fornas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166036","display_name":"Instituto de Investigaci\u00f3n Sanitaria La Fe","ror":"https://ror.org/05n7v5997","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I4210166036"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"E. Fornas","raw_affiliation_strings":["Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain","institution_ids":["https://openalex.org/I4210166036"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058007777","display_name":"Jaime Renau\u2010Piqueras","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166036","display_name":"Instituto de Investigaci\u00f3n Sanitaria La Fe","ror":"https://ror.org/05n7v5997","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I4210166036"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"J. Renau\u2010Piqueras","raw_affiliation_strings":["Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain","institution_ids":["https://openalex.org/I4210166036"]}]},{"author_position":"middle","author":{"id":null,"display_name":"A. Fortea","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166036","display_name":"Instituto de Investigaci\u00f3n Sanitaria La Fe","ror":"https://ror.org/05n7v5997","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I4210166036"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"A. Fortea","raw_affiliation_strings":["Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain","institution_ids":["https://openalex.org/I4210166036"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108600188","display_name":"Fernando Mayordomo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166036","display_name":"Instituto de Investigaci\u00f3n Sanitaria La Fe","ror":"https://ror.org/05n7v5997","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I4210166036"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"F. Mayordomo","raw_affiliation_strings":["Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain","institution_ids":["https://openalex.org/I4210166036"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5036733067","display_name":"Enrique Alborch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166036","display_name":"Instituto de Investigaci\u00f3n Sanitaria La Fe","ror":"https://ror.org/05n7v5997","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I4210166036"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"E. Alborch","raw_affiliation_strings":["Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Investigaci\u00f3n Hospital \u201cLa Fe\u201d Avenida de Campanar, 21 Valencia 46009 Spain","institution_ids":["https://openalex.org/I4210166036"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210166036"],"apc_list":{"value":3589,"currency":"USD","value_usd":3589},"apc_paid":null,"fwci":0.3662,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.49669166,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"28","issue":"10","first_page":"929","last_page":"935"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11618","display_name":"Cholesterol and Lipid Metabolism","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11618","display_name":"Cholesterol and Lipid Metabolism","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.023800000548362732,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13736","display_name":"Steroid Chemistry and Biochemistry","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/25-hydroxycholesterol","display_name":"25-hydroxycholesterol","score":0.9819999933242798},{"id":"https://openalex.org/keywords/electron-microscopy-specimen-preparation","display_name":"electron microscopy specimen preparation","score":0.6470000147819519},{"id":"https://openalex.org/keywords/tissue-fixation","display_name":"tissue fixation","score":0.8479999899864197},{"id":"https://openalex.org/keywords/filipin","display_name":"filipin","score":0.7609999775886536},{"id":"https://openalex.org/keywords/osmium-fixation","display_name":"osmium fixation","score":0.8230000138282776},{"id":"https://openalex.org/keywords/digitonin","display_name":"digitonin","score":0.9850000143051147},{"id":"https://openalex.org/keywords/saponins","display_name":"saponins","score":0.9919999837875366},{"id":"https://openalex.org/keywords/liver","display_name":"liver","score":0.6869999766349792},{"id":"https://openalex.org/keywords/aorta","display_name":"aorta","score":0.9980000257492065}],"concepts":[{"id":"https://openalex.org/C2778920010","wikidata":"https://www.wikidata.org/wiki/Q410968","display_name":"Digitonin","level":3,"score":0.775862991809845},{"id":"https://openalex.org/C2778163477","wikidata":"https://www.wikidata.org/wiki/Q43656","display_name":"Cholesterol","level":2,"score":0.7012885212898254},{"id":"https://openalex.org/C2779630084","wikidata":"https://www.wikidata.org/wiki/Q2073868","display_name":"Filipin","level":3,"score":0.6624177694320679},{"id":"https://openalex.org/C22894154","wikidata":"https://www.wikidata.org/wiki/Q15483409","display_name":"Lipidology","level":3,"score":0.5974427461624146},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5754027962684631},{"id":"https://openalex.org/C382228","wikidata":"https://www.wikidata.org/wiki/Q849994","display_name":"Clinical chemistry","level":2,"score":0.5133592486381531},{"id":"https://openalex.org/C2779980429","wikidata":"https://www.wikidata.org/wiki/Q101004","display_name":"Aorta","level":2,"score":0.49087268114089966},{"id":"https://openalex.org/C2779424567","wikidata":"https://www.wikidata.org/wiki/Q422021","display_name":"Osmium tetroxide","level":3,"score":0.45651957392692566},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.44326502084732056},{"id":"https://openalex.org/C540285154","wikidata":"https://www.wikidata.org/wiki/Q751","display_name":"Osmium","level":4,"score":0.4397527575492859},{"id":"https://openalex.org/C2778718757","wikidata":"https://www.wikidata.org/wiki/Q143623","display_name":"Sterol","level":3,"score":0.41540202498435974},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3617478013038635},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3365901708602905},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3160991370677948},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.17548248171806335},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.1590120792388916},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C555196967","wikidata":"https://www.wikidata.org/wiki/Q1086","display_name":"Ruthenium","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001011","descriptor_name":"Aorta","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001011","descriptor_name":"Aorta","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001011","descriptor_name":"Aorta","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002784","descriptor_name":"Cholesterol","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002784","descriptor_name":"Cholesterol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004072","descriptor_name":"Digitonin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005372","descriptor_name":"Filipin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006652","descriptor_name":"Histological Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006888","descriptor_name":"Hydroxycholesterols","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006888","descriptor_name":"Hydroxycholesterols","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009131","descriptor_name":"Muscle, Smooth, Vascular","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D009131","descriptor_name":"Muscle, Smooth, Vascular","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009131","descriptor_name":"Muscle, Smooth, Vascular","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D009992","descriptor_name":"Osmium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012503","descriptor_name":"Saponins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/bf02537503","doi":"https://doi.org/10.1007/bf02537503","is_oa":false,"landing_page_url":"https://doi.org/10.1007/bf02537503","pdf_url":null,"source":{"id":"https://openalex.org/S115623499","display_name":"Lipids","issn_l":"0024-4201","issn":["0024-4201","1558-9307"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lipids","raw_type":"journal-article"},{"id":"pmid:8246694","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8246694","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lipids","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W379849480","https://openalex.org/W573577085","https://openalex.org/W1520917818","https://openalex.org/W1967440029","https://openalex.org/W1974351314","https://openalex.org/W1994827558","https://openalex.org/W2000030956","https://openalex.org/W2001390747","https://openalex.org/W2007674547","https://openalex.org/W2007704417","https://openalex.org/W2010064670","https://openalex.org/W2013709390","https://openalex.org/W2017343466","https://openalex.org/W2022530131","https://openalex.org/W2024562547","https://openalex.org/W2026975428","https://openalex.org/W2030643617","https://openalex.org/W2039219163","https://openalex.org/W2043803308","https://openalex.org/W2044903529","https://openalex.org/W2051628968","https://openalex.org/W2057820745","https://openalex.org/W2060581819","https://openalex.org/W2064065403","https://openalex.org/W2068830714","https://openalex.org/W2080048000","https://openalex.org/W2083900434","https://openalex.org/W2086573861","https://openalex.org/W2094926434","https://openalex.org/W2100822669","https://openalex.org/W2115220143","https://openalex.org/W2128488935","https://openalex.org/W2158607960","https://openalex.org/W2171605521","https://openalex.org/W2334242861","https://openalex.org/W2396738159","https://openalex.org/W2397833734","https://openalex.org/W2407556527","https://openalex.org/W2419017908","https://openalex.org/W2497432837","https://openalex.org/W2885860780","https://openalex.org/W3016893200","https://openalex.org/W3161349648","https://openalex.org/W3166029991"],"related_works":["https://openalex.org/W2022530131","https://openalex.org/W2158607960","https://openalex.org/W3035808384","https://openalex.org/W7101033428","https://openalex.org/W2000358836","https://openalex.org/W2099292414","https://openalex.org/W2067119960","https://openalex.org/W1981483439","https://openalex.org/W2007526152","https://openalex.org/W2002548012"],"abstract_inverted_index":{"Sprague-Dawley":[0],"rats":[1],"were":[2,22,30,59,86],"injected":[3],"intraperitoneally":[4],"with":[5],"[1":[6],"alpha,":[7],"2":[8],"alpha(n)-3H]cholesterol":[9],"or":[10,79],"25-hydroxy-[26,27-3H]cholesterol,":[11],"and":[12,14,19,47,55,83,88],"one":[13],"five":[15],"days":[16],"later":[17],"liver":[18,82],"aortic":[20,84],"tissues":[21,34,85],"fixed.":[23],"The":[24],"extent":[25],"to":[26],"which":[27],"these":[28],"sterols":[29],"lost":[31],"from":[32],"the":[33,64],"during":[35],"preparation":[36],"for":[37],"electron":[38],"microscopy":[39],"(EM)":[40],"was":[41],"examined":[42,66],"utilizing":[43],"different":[44],"fixation":[45,72],"procedures":[46,65,73],"various":[48],"protective":[49],"agents.":[50],"Radioactive":[51],"tracers,":[52],"scintillation":[53],"counting":[54],"standard":[56],"EM":[57],"techniques":[58],"used.":[60],"Although":[61],"most":[62],"of":[63,77],"caused":[67],"major":[68],"lipid":[69],"losses,":[70],"useful":[71],"that":[74],"allow":[75],"retention":[76],"cholesterol":[78],"25-hydroxy-cholesterol":[80],"in":[81],"found":[87],"are":[89],"described":[90],"here.":[91]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}