{"id":"https://openalex.org/W2081141982","doi":"https://doi.org/10.1371/journal.pone.0006668","title":"Assessing the Contribution of Heme-Iron Acquisition to Staphylococcus aureus Pneumonia Using Computed Tomography","display_name":"Assessing the Contribution of Heme-Iron Acquisition to Staphylococcus aureus Pneumonia Using Computed Tomography","publication_year":2009,"publication_date":"2009-08-17","ids":{"openalex":"https://openalex.org/W2081141982","doi":"https://doi.org/10.1371/journal.pone.0006668","mag":"2081141982","pmid":"https://pubmed.ncbi.nlm.nih.gov/19688098","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2723934"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0006668","doi":"https://doi.org/10.1371/journal.pone.0006668","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0006668","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006668&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006668&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"William Jeffrey Mason","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William Jeffrey Mason","raw_affiliation_strings":["Division of Infectious Diseases, Department of Internal Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Infectious Diseases, Department of Internal Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082616273","display_name":"Eric Patrick Skaar","orcid":"https://orcid.org/0000-0001-5094-8105","observed_orcids":["https://orcid.org/0000-0001-5094-8105","https://orcid.org/0000-0002-6533-2458"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Eric Patrick Skaar","raw_affiliation_strings":["Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America","institution_ids":["https://openalex.org/I200719446"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5082616273"],"corresponding_institution_ids":["https://openalex.org/I200719446"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":2.1378,"has_fulltext":true,"cited_by_count":27,"citation_normalized_percentile":{"value":0.8611901,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"4","issue":"8","first_page":"e6668","last_page":"e6668"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.8544999957084656,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.8544999957084656,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.03550000116229057,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.023600000888109207,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.9909999966621399},{"id":"https://openalex.org/keywords/staphylococcal-pneumonia","display_name":"staphylococcal pneumonia","score":0.9190000295639038},{"id":"https://openalex.org/keywords/isdb","display_name":"ISDB","score":0.6549999713897705},{"id":"https://openalex.org/keywords/isdh","display_name":"IsdH","score":0.953000009059906},{"id":"https://openalex.org/keywords/murine-pneumonia-model","display_name":"murine pneumonia model","score":0.6740000247955322},{"id":"https://openalex.org/keywords/bacterial-virulence","display_name":"bacterial virulence","score":0.7490000128746033},{"id":"https://openalex.org/keywords/bacterial-load","display_name":"bacterial load","score":0.6779999732971191},{"id":"https://openalex.org/keywords/hemoglobin-binding","display_name":"hemoglobin binding","score":0.6919999718666077}],"concepts":[{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.8925998210906982},{"id":"https://openalex.org/C2777914695","wikidata":"https://www.wikidata.org/wiki/Q12192","display_name":"Pneumonia","level":2,"score":0.7471885085105896},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.7212681770324707},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5521343350410461},{"id":"https://openalex.org/C2777714996","wikidata":"https://www.wikidata.org/wiki/Q7886","display_name":"Lung","level":2,"score":0.5290312767028809},{"id":"https://openalex.org/C2778917026","wikidata":"https://www.wikidata.org/wiki/Q43041","display_name":"Hemoglobin","level":2,"score":0.4897580146789551},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.47479763627052307},{"id":"https://openalex.org/C2777334864","wikidata":"https://www.wikidata.org/wiki/Q34879","display_name":"Staphylococcal infections","level":4,"score":0.425042986869812},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3826700448989868},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.28843480348587036},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.26604318618774414},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25968223810195923},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.07318058609962463},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.059262603521347046},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004195","descriptor_name":"Disease Models, Animal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006418","descriptor_name":"Heme","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000472","qualifier_name":"pathogenicity","is_major_topic":false},{"descriptor_ui":"D014057","descriptor_name":"Tomography, X-Ray Computed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018410","descriptor_name":"Pneumonia, Bacterial","qualifier_ui":"Q000000981","qualifier_name":"diagnostic imaging","is_major_topic":false},{"descriptor_ui":"D018410","descriptor_name":"Pneumonia, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018410","descriptor_name":"Pneumonia, Bacterial","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1371/journal.pone.0006668","doi":"https://doi.org/10.1371/journal.pone.0006668","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0006668","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006668&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2723934","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2723934","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:19688098","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19688098","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.356.5602","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.5602","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/ca/d7/PLoS_One_2009_Aug_18_4(8)_e6668.tar.gz","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:402d18577e514132b101d92b31a01f17","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/402d18577e514132b101d92b31a01f17","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 4, Iss 8, p e6668 (2009)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0006668","doi":"https://doi.org/10.1371/journal.pone.0006668","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0006668","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006668&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.979200005531311}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4448629141","display_name":null,"funder_award_id":"R01 A1069233","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"},{"id":"https://openalex.org/G864420322","display_name":null,"funder_award_id":"T32 AI-07474-13","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G9000904142","display_name":"Vanderbilt Infection Pathogenesis and Epidemiology Research Training Program","funder_award_id":"T32AI007474","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320309151","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2081141982.pdf","grobid_xml":"https://content.openalex.org/works/W2081141982.grobid-xml"},"referenced_works_count":10,"referenced_works":["https://openalex.org/W1979135961","https://openalex.org/W1979194107","https://openalex.org/W1980482357","https://openalex.org/W2001277616","https://openalex.org/W2011450595","https://openalex.org/W2094045382","https://openalex.org/W2107509252","https://openalex.org/W2112652622","https://openalex.org/W2122822084","https://openalex.org/W2123564837"],"related_works":["https://openalex.org/W2468339878","https://openalex.org/W2124299428","https://openalex.org/W2107509252","https://openalex.org/W2158715097","https://openalex.org/W2094045382","https://openalex.org/W1979194107","https://openalex.org/W2018849855","https://openalex.org/W7067650223","https://openalex.org/W2294314824","https://openalex.org/W4286852565"],"abstract_inverted_index":{"BACKGROUND:":[0],"S.":[1,115,124,160,255],"aureus":[2,125,161],"acquires":[3],"heme-iron":[4,33,91,252,263],"using":[5,217],"the":[6,14,45,52,87,94,195,218,230,267,288],"iron":[7],"regulated":[8],"surface":[9],"determinant":[10],"(Isd)":[11],"system":[12,17],"and":[13,57,96,131,136,153,163,233,290],"heme":[15],"transport":[16],"(Hts)":[18],"with":[19,105,121,159,206],"both":[20],"systems":[21],"showing":[22],"critical":[23],"importance":[24],"in":[25,129,168,173,226,229,241],"systemic":[26,213,270],"models":[27],"of":[28,32,47,54,63,82,90,114,184,209,215,238,269,277,292],"infection.":[29],"The":[30,236],"contribution":[31,89],"acquisition":[34,92,264],"to":[35,60,71,78,85,180,200,266,284],"staphylococcal":[36,55,83,239,271],"pneumonia":[37,56,84,240],"has":[38,67],"not":[39,68,166,189,246],"yet":[40],"been":[41,69],"elucidated.":[42],"In":[43,273],"addition,":[44,274],"use":[46],"computed":[48,148],"tomography":[49,149],"(CT)":[50],"for":[51,287],"evaluation":[53],"its":[58],"correlation":[59],"pathologic":[61,207],"examination":[62],"infected":[64,120,185],"lung":[65],"tissue":[66],"performed":[70],"date.":[72],"We":[73],"have":[74],"applied":[75],"CT-based":[76],"imaging":[77,183,276],"a":[79,169,212,222],"murine":[80,243,278],"model":[81,214,244,259],"determine":[86],"virulence":[88],"through":[93],"Hts":[95],"Isd":[97],"systems.":[98],"METHODOLOGY/PRINCIPAL":[99],"FINDINGS:":[100],"Mice":[101],"were":[102,139,156],"intranasally":[103],"inoculated":[104],"approximately":[106],"1.0":[107],"x":[108],"10(8)":[109],"colony":[110],"forming":[111],"units":[112],"(CFU)":[113],"aureus.":[116,256],"Lungs":[117],"from":[118],"mice":[119,186],"wild":[122,201],"type":[123],"or":[126,134,175,251],"strains":[127,197],"deficient":[128],"isdB":[130],"isdH":[132],"(DeltaisdBH)":[133],"htsA":[135],"isdE":[137],"(DeltahtsADeltaisdE)":[138],"harvested":[140],"at":[141],"24":[142],"hours.":[143],"Histology,":[144],"radiographic":[145],"appearance":[146],"by":[147],"(CT),":[150],"percent":[151],"mortality":[152,174],"bacterial":[154,176,227],"burden":[155,177,228],"evaluated.":[157],"Infection":[158],"DeltaisdBH":[162],"DeltahtsADeltaisdE":[164,219],"did":[165,188,204],"result":[167],"statistically":[170,223],"significant":[171,224],"difference":[172,193],"as":[178],"compared":[179,199],"controls.":[181],"CT":[182,275],"also":[187],"reveal":[190,261],"an":[191,281],"appreciable":[192],"between":[194],"various":[196],"when":[198],"type,":[202],"but":[203],"correlate":[205],"findings":[208],"pneumonia.":[210,293],"However,":[211,257],"infection":[216],"strain":[220],"revealed":[221],"decrease":[225],"lung,":[231],"heart":[232],"kidneys.":[234],"CONCLUSIONS/SIGNIFICANCE:":[235],"development":[237],"this":[242,258],"is":[245,280],"dependent":[247],"on":[248],"hemoglobin":[249],"binding":[250],"uptake":[253],"into":[254],"does":[260],"that":[262],"contributes":[265],"pathogenesis":[268],"infections.":[272],"lungs":[279],"attractive":[282],"adjunct":[283],"histologic":[285],"analysis":[286],"confirmation":[289],"staging":[291]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}