{"id":"https://openalex.org/W2080869477","doi":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","title":"Comparison of the Kinetics of [3H]Diazepam and [3H]Flunitrazepam Binding to Cortical Synaptosomal Membranes","display_name":"Comparison of the Kinetics of [3H]Diazepam and [3H]Flunitrazepam Binding to Cortical Synaptosomal Membranes","publication_year":1982,"publication_date":"1982-12-01","ids":{"openalex":"https://openalex.org/W2080869477","doi":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","mag":"2080869477","pmid":"https://pubmed.ncbi.nlm.nih.gov/6128377"},"language":"en","primary_location":{"id":"doi:10.1111/j.1471-4159.1982.tb08008.x","doi":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","pdf_url":null,"source":{"id":"https://openalex.org/S135837084","display_name":"Journal of Neurochemistry","issn_l":"0022-3042","issn":["0022-3042","1471-4159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neurochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110077432","display_name":"Ted H. Chiu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ted H. Chiu","raw_affiliation_strings":["Department of Pharmacology and Therapeutics, Medical College of Ohio, Toledo, Ohio, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics, Medical College of Ohio, Toledo, Ohio, U.S.A","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045289644","display_name":"Howard C. Rosenberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Howard C. Rosenberg","raw_affiliation_strings":["Department of Pharmacology and Therapeutics, Medical College of Ohio, Toledo, Ohio, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics, Medical College of Ohio, Toledo, Ohio, U.S.A","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5110077432"],"corresponding_institution_ids":[],"apc_list":{"value":5330,"currency":"USD","value_usd":5330},"apc_paid":null,"fwci":0.9542,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.69022119,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"39","issue":"6","first_page":"1716","last_page":"1725"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/diazepam","display_name":"diazepam","score":0.8230000138282776},{"id":"https://openalex.org/keywords/flunitrazepam","display_name":"flunitrazepam","score":1.0},{"id":"https://openalex.org/keywords/benzodiazepine-receptor","display_name":"benzodiazepine receptor","score":0.9419999718666077},{"id":"https://openalex.org/keywords/receptor-binding-kinetics","display_name":"receptor binding kinetics","score":0.7509999871253967},{"id":"https://openalex.org/keywords/gaba","display_name":"GABA","score":0.4410000145435333},{"id":"https://openalex.org/keywords/sodium-chloride","display_name":"sodium chloride","score":0.8180000185966492},{"id":"https://openalex.org/keywords/pentobarbital","display_name":"pentobarbital","score":0.9879999756813049},{"id":"https://openalex.org/keywords/ro-15-1788","display_name":"Ro 15-1788","score":0.9480000138282776},{"id":"https://openalex.org/keywords/clonazepam","display_name":"clonazepam","score":0.9459999799728394},{"id":"https://openalex.org/keywords/receptor-dissociation","display_name":"receptor dissociation","score":0.5450000166893005},{"id":"https://openalex.org/keywords/receptor-interaction","display_name":"receptor interaction","score":0.4880000054836273}],"concepts":[{"id":"https://openalex.org/C2781442855","wikidata":"https://www.wikidata.org/wiki/Q62947","display_name":"Flunitrazepam","level":4,"score":0.9504274129867554},{"id":"https://openalex.org/C2776175493","wikidata":"https://www.wikidata.org/wiki/Q210402","display_name":"Diazepam","level":2,"score":0.9090876579284668},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8140444159507751},{"id":"https://openalex.org/C2776755955","wikidata":"https://www.wikidata.org/wiki/Q409632","display_name":"Pentobarbital","level":2,"score":0.7455400228500366},{"id":"https://openalex.org/C2779426884","wikidata":"https://www.wikidata.org/wiki/Q407988","display_name":"Clonazepam","level":2,"score":0.634996771812439},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.6345337629318237},{"id":"https://openalex.org/C2778173252","wikidata":"https://www.wikidata.org/wiki/Q83871","display_name":"Benzodiazepine","level":3,"score":0.6250237822532654},{"id":"https://openalex.org/C168258287","wikidata":"https://www.wikidata.org/wiki/Q60147","display_name":"GABAA receptor","level":3,"score":0.44545578956604004},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.42891258001327515},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.39231613278388977},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3322139382362366},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2755492925643921},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.2611604928970337},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.07038769125938416},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.06086036562919617}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001570","descriptor_name":"Benzodiazepinones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002540","descriptor_name":"Cerebral Cortex","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003975","descriptor_name":"Diazepam","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005442","descriptor_name":"Flumazenil","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005445","descriptor_name":"Flunitrazepam","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005680","descriptor_name":"gamma-Aminobutyric Acid","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010424","descriptor_name":"Pentobarbital","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013570","descriptor_name":"Synaptic Membranes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014151","descriptor_name":"Anti-Anxiety Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1471-4159.1982.tb08008.x","doi":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1471-4159.1982.tb08008.x","pdf_url":null,"source":{"id":"https://openalex.org/S135837084","display_name":"Journal of Neurochemistry","issn_l":"0022-3042","issn":["0022-3042","1471-4159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neurochemistry","raw_type":"journal-article"},{"id":"pmid:6128377","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6128377","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of neurochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4000000059604645}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2246373489","display_name":"BENZODIAZEPINE RECEPTOR  REGULATION AND PURIFICATION","funder_award_id":"R01NS016595","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1490928184","https://openalex.org/W1504981459","https://openalex.org/W1562567109","https://openalex.org/W1575177681","https://openalex.org/W1684853394","https://openalex.org/W1775749144","https://openalex.org/W1965666249","https://openalex.org/W1968332855","https://openalex.org/W1978342290","https://openalex.org/W1980979559","https://openalex.org/W1983334593","https://openalex.org/W1991861023","https://openalex.org/W1994148459","https://openalex.org/W1994785056","https://openalex.org/W1997470820","https://openalex.org/W2007911341","https://openalex.org/W2014066806","https://openalex.org/W2017070484","https://openalex.org/W2025904162","https://openalex.org/W2026290882","https://openalex.org/W2032372670","https://openalex.org/W2041207218","https://openalex.org/W2042232934","https://openalex.org/W2046092232","https://openalex.org/W2056945518","https://openalex.org/W2057981021","https://openalex.org/W2070136897","https://openalex.org/W2078206080","https://openalex.org/W2091783477","https://openalex.org/W2093810122","https://openalex.org/W2116644619","https://openalex.org/W2198583935","https://openalex.org/W2326434939","https://openalex.org/W2397465724","https://openalex.org/W2405639989","https://openalex.org/W2413743735","https://openalex.org/W4244017727","https://openalex.org/W6687772812"],"related_works":["https://openalex.org/W2405639989","https://openalex.org/W7100343175","https://openalex.org/W2161510399","https://openalex.org/W2410578239","https://openalex.org/W1998675359","https://openalex.org/W2033706090","https://openalex.org/W1982904143","https://openalex.org/W2511561063","https://openalex.org/W1916068919","https://openalex.org/W2198583935"],"abstract_inverted_index":{"[3H]Diazepam":[0,85,96,136],"and":[1,7,23,34,55,90,109,149,169,185],"[3H]flunitrazepam":[2],"([3H]FNP)":[3],"binding":[4,28,60,191],"to":[5,210],"washed":[6],"frozen":[8],"synaptosomal":[9],"membranes":[10],"from":[11,189],"rat":[12],"cerebral":[13],"cortex":[14],"were":[15],"compared.":[16],"In":[17],"Tris-citrate":[18],"buffer,":[19],"gamma-aminobutyric":[20],"acid":[21],"(GABA)":[22],"NaCl":[24,54,164],"both":[25,36,83],"increased":[26,154],"[3H]diazepam":[27,49],"more":[29,76],"than":[30,68,93,105,145,199],"[3H]FNP":[31,94,119],"binding.":[32],"GABA":[33,148,168],"pentobarbital":[35],"enhanced":[37],"this":[38],"effect":[39,162],"of":[40,43,48,53,107,115,157,163,177,207],"NaCl.":[41],"Because":[42],"the":[44,51,57,65,70,79,155,174,205,208,211],"extremely":[45],"rapid":[46],"dissociation":[47,120,137],"in":[50,82,181,203],"absence":[52],"GABA,":[56],"Bmax":[58,81],"(maximal":[59],"capacity)":[61],"was":[62,91,103,121,143,165],"smaller":[63],"by":[64,69,125,167],"filtration":[66],"assay":[67],"centrifugation":[71],"assay.":[72],"[3H]FNP,":[73,108],"which":[74,98,131],"dissociates":[75],"slowly,":[77],"had":[78,87,100,111],"same":[80],"assays.":[84],"association":[86],"two":[88,101,182],"components,":[89,102],"faster":[92,104],"association.":[95],"dissociation,":[97],"also":[99,110],"that":[106],"a":[112,133],"greater":[113],"fraction":[114],"rapidly":[116],"dissociating":[117,159],"species.":[118,160],"similar":[122],"when":[123],"initiated":[124],"diazepam,":[126],"flunitrazepam,":[127,140],"clonazepam,":[128],"or":[129,141],"Ro15-1788,":[130,139],"is":[132],"benzodiazepine":[134,178],"antagonist.":[135],"with":[138,146],"clonazepam":[142],"slower":[144],"diazepam.":[147],"NaCl,":[150],"but":[151],"not":[152],"pentobarbital,":[153],"percentage":[156],"slowly":[158],"This":[161],"potentiated":[166],"pentobarbital.":[170],"The":[171],"results":[172],"support":[173],"cyclic":[175],"model":[176],"receptors":[179],"existing":[180],"interconvertible":[183],"conformations,":[184],"suggest":[186],"that,":[187],"distinct":[188],"their":[190],"affinity,":[192],"some":[193],"ligands":[194],"(like":[195,201],"flunitrazepam)":[196],"are":[197],"better":[198],"others":[200],"diazepam)":[202],"inducing":[204],"conversion":[206],"receptor":[209],"higher-affinity":[212],"state.":[213]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}