{"id":"https://openalex.org/W2080042971","doi":"https://doi.org/10.1021/bi00030a017","title":"Evidence for class-specific discrimination of a semiconserved base pair by tRNA synthetases","display_name":"Evidence for class-specific discrimination of a semiconserved base pair by tRNA synthetases","publication_year":1995,"publication_date":"1995-08-01","ids":{"openalex":"https://openalex.org/W2080042971","doi":"https://doi.org/10.1021/bi00030a017","mag":"2080042971","pmid":"https://pubmed.ncbi.nlm.nih.gov/7542924"},"language":"en","primary_location":{"id":"doi:10.1021/bi00030a017","doi":"https://doi.org/10.1021/bi00030a017","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00030a017","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100783258","display_name":"Hongjian Liu","orcid":"https://orcid.org/0000-0002-3346-9193","observed_orcids":["https://orcid.org/0000-0002-3346-9193"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hongjian Liu","raw_affiliation_strings":["Department of Chemistry, University of Minnesota, Minneapolis 55455, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Minnesota, Minneapolis 55455, USA","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055637475","display_name":"Jason Kessler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jason Kessler","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Richard Peterson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Richard Peterson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5049506879","display_name":"Karin Musier\u2010Forsyth","orcid":"https://orcid.org/0000-0002-0354-4172","observed_orcids":["https://orcid.org/0000-0002-0354-4172"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Karin Musier-Forsyth","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.2478,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.4991274,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"34","issue":"30","first_page":"9795","last_page":"9800"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aminoacyl-trna-synthetase","display_name":"aminoacyl-tRNA synthetase","score":0.9950000047683716},{"id":"https://openalex.org/keywords/trna-recognition","display_name":"tRNA recognition","score":0.6990000009536743},{"id":"https://openalex.org/keywords/trna-acceptor-stem","display_name":"tRNA acceptor stem","score":0.9100000262260437},{"id":"https://openalex.org/keywords/alanyl-trna-synthetase","display_name":"alanyl-tRNA synthetase","score":0.8479999899864197},{"id":"https://openalex.org/keywords/prolyl-trna-synthetase","display_name":"prolyl-tRNA synthetase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/histidyl-trna-synthetase","display_name":"histidyl-tRNA synthetase","score":1.0},{"id":"https://openalex.org/keywords/aminoacylation","display_name":"aminoacylation","score":0.6380000114440918},{"id":"https://openalex.org/keywords/escherichia-coli-trna","display_name":"Escherichia coli tRNA","score":0.8169999718666077}],"concepts":[{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.7035349607467651},{"id":"https://openalex.org/C2779315393","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Aminoacyl tRNA synthetase","level":5,"score":0.5089459419250488},{"id":"https://openalex.org/C42058472","wikidata":"https://www.wikidata.org/wiki/Q810214","display_name":"Base (topology)","level":2,"score":0.5086787343025208},{"id":"https://openalex.org/C2777212361","wikidata":"https://www.wikidata.org/wiki/Q5127848","display_name":"Class (philosophy)","level":2,"score":0.47875967621803284},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.423813134431839},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3993931710720062},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38783547282218933},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.348411500453949},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.22082257270812988},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.19778335094451904},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.14089375734329224},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1193891167640686},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.10557758808135986},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000412","descriptor_name":"Alanine-tRNA Ligase","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D000412","descriptor_name":"Alanine-tRNA Ligase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000412","descriptor_name":"Alanine-tRNA Ligase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001482","descriptor_name":"Base Composition","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012344","descriptor_name":"RNA, Transfer, Ala","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012344","descriptor_name":"RNA, Transfer, Ala","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012361","descriptor_name":"RNA, Transfer, Pro","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012361","descriptor_name":"RNA, Transfer, Pro","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012366","descriptor_name":"RNA, Transfer, Val","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012366","descriptor_name":"RNA, Transfer, Val","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00030a017","doi":"https://doi.org/10.1021/bi00030a017","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00030a017","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:7542924","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7542924","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5471514413","display_name":null,"funder_award_id":"GM49928","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320312060","display_name":"Francis Crick Institute","ror":"https://ror.org/04tnbqb63"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W617859195","https://openalex.org/W1498439983","https://openalex.org/W1533677474","https://openalex.org/W1772912839","https://openalex.org/W1950941644","https://openalex.org/W1970421002","https://openalex.org/W1975940326","https://openalex.org/W1977663290","https://openalex.org/W1978925476","https://openalex.org/W1979381401","https://openalex.org/W1981529363","https://openalex.org/W1982261101","https://openalex.org/W1984854823","https://openalex.org/W1985687578","https://openalex.org/W1990700979","https://openalex.org/W2005894130","https://openalex.org/W2006074942","https://openalex.org/W2016354070","https://openalex.org/W2017980451","https://openalex.org/W2019729932","https://openalex.org/W2023880708","https://openalex.org/W2024420567","https://openalex.org/W2030801747","https://openalex.org/W2033843196","https://openalex.org/W2036931150","https://openalex.org/W2039911841","https://openalex.org/W2057481603","https://openalex.org/W2057781025","https://openalex.org/W2060508247","https://openalex.org/W2062262417","https://openalex.org/W2071674430","https://openalex.org/W2076796893","https://openalex.org/W2078216263","https://openalex.org/W2083812890","https://openalex.org/W2085791091","https://openalex.org/W2093109164","https://openalex.org/W2094757867","https://openalex.org/W2103947911","https://openalex.org/W2127749120","https://openalex.org/W2136915898","https://openalex.org/W2139305314","https://openalex.org/W2155824962","https://openalex.org/W2482664831"],"related_works":["https://openalex.org/W929289056","https://openalex.org/W1976956092","https://openalex.org/W2167163708","https://openalex.org/W1602214435","https://openalex.org/W2030328702","https://openalex.org/W2328098545","https://openalex.org/W2401127910","https://openalex.org/W2012804004","https://openalex.org/W1523120820","https://openalex.org/W2127005277"],"abstract_inverted_index":{"Aminoacyl-tRNA":[0],"synthetases":[1,38,129,144],"have":[2,25,44],"been":[3,26,45],"divided":[4],"into":[5],"two":[6,13],"classes":[7],"based":[8],"on":[9,141],"the":[10,50,55,58,63,67,76,84,98,106,151,187,195,201,208],"existence":[11],"of":[12,31,57,91,108,164,190,197],"structurally":[14],"distinct":[15],"active":[16],"sites.":[17],"To":[18],"date,":[19],"few":[20],"class-specific":[21,172],"tRNA":[22,42,59,74,175],"recognition":[23],"features":[24],"elucidated.":[27],"High-resolution":[28],"X-ray":[29],"structures":[30],"representative":[32],"class":[33,36,51,68,123,142,156],"I":[34,52,143,157],"and":[35,127,145],"II":[37,69,124],"complexed":[39],"to":[40,132,162,181,207],"cognate":[41,73],"substrates":[43],"solved.":[46],"In":[47],"these":[48],"structures,":[49],"enzyme":[53,153],"approaches":[54,71],"end":[56],"acceptor":[60,176],"stem":[61,177],"from":[62,75],"minor-groove":[64],"side,":[65],"while":[66],"synthetase":[70,198],"its":[72],"major-groove":[77],"side.":[78],"This":[79,166],"distinction":[80],"is":[81,112],"reflected":[82,183],"in":[83,105,114,116,174,186,194],"different":[85],"initial":[86,188],"sites":[87],"(2'-":[88],"or":[89],"3'-OH)":[90],"amino":[92,209],"acid":[93,210],"attachment.":[94],"The":[95],"role":[96],"that":[97,122,155,179],"semiconserved":[99,202],"G1.C72":[100,203],"terminal":[101],"base":[102,204],"pair":[103,205],"plays":[104],"aminoacylation":[107,191],"Escherichia":[109],"coli":[110],"tRNAs":[111],"probed":[113],"this":[115],"vitro":[117],"study.":[118],"We":[119],"show":[120,159],"here":[121,149],"alanyl-,":[125],"prolyl-,":[126],"histidyl-tRNA":[128],"are":[130],"sensitive":[131],"changes":[133],"at":[134],"position":[135],"1":[136],"x":[137],"72.":[138],"Previous":[139],"work":[140,167],"new":[146,169],"data":[147],"presented":[148],"with":[150,200],"valine-specific":[152],"indicate":[154],"enzymes":[158],"little":[160],"sensitivity":[161],"replacements":[163],"G1.C72.":[165],"provides":[168],"evidence":[170],"for":[171],"differences":[173],"interactions":[178],"appear":[180],"be":[182],"not":[184],"only":[185],"site":[189],"but":[192],"also":[193],"mode":[196],"interaction":[199],"proximal":[206],"attachment":[211],"site.":[212]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}