{"id":"https://openalex.org/W2078790282","doi":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","title":"Transport characteristics of human proximal tubule cells in primary culture","display_name":"Transport characteristics of human proximal tubule cells in primary culture","publication_year":1997,"publication_date":"1997-04-01","ids":{"openalex":"https://openalex.org/W2078790282","doi":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","mag":"2078790282"},"language":"en","primary_location":{"id":"doi:10.1111/j.1440-1797.1997.tb00213.x","doi":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","pdf_url":null,"source":{"id":"https://openalex.org/S99619495","display_name":"Nephrology","issn_l":"1320-5358","issn":["1320-5358","1440-1797"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nephrology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043394711","display_name":"David Wayne Johnson","orcid":"https://orcid.org/0000-0001-5491-3460","observed_orcids":["https://orcid.org/0000-0001-5491-3460","https://orcid.org/0000-0001-8453-7849","https://orcid.org/0000-0001-6932-2460"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"David W JOHNSON","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052066809","display_name":"Bronwyn K. Brew","orcid":"https://orcid.org/0000-0002-9885-8261","observed_orcids":["https://orcid.org/0000-0002-9885-8261"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Bronwyn K BREW","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037639469","display_name":"Philip Poronnik","orcid":"https://orcid.org/0000-0002-6246-528X","observed_orcids":["https://orcid.org/0000-0002-6246-528X"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Philip PORONNIK","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103580420","display_name":"David I. Cook","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"David I COOK","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013916194","display_name":"Akos Z. Gyory","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Akos Z GY\u00d6RY","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078462683","display_name":"Michael John Field","orcid":"https://orcid.org/0000-0003-1310-5074","observed_orcids":["https://orcid.org/0000-0003-1310-5074"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Michael J FIELD","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016584401","display_name":"Carol A. Pollock","orcid":"https://orcid.org/0000-0001-7167-6495","observed_orcids":["https://orcid.org/0000-0001-7167-6495"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Carol A POLLOCK","raw_affiliation_strings":["Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and Physiology, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5016584401"],"corresponding_institution_ids":["https://openalex.org/I129604602"],"apc_list":{"value":4110,"currency":"USD","value_usd":4110},"apc_paid":null,"fwci":1.5309,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.82673072,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"3","issue":"2","first_page":"183","last_page":"194"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.023900000378489494,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.906000018119812},{"id":"https://openalex.org/keywords/polarized-transport","display_name":"polarized transport","score":0.671999990940094},{"id":"https://openalex.org/keywords/sodium-glucose-cotransport","display_name":"sodium-glucose cotransport","score":0.7829999923706055},{"id":"https://openalex.org/keywords/organic-anion-transport","display_name":"organic anion transport","score":0.9819999933242798},{"id":"https://openalex.org/keywords/organic-cation-transport","display_name":"organic cation transport","score":0.9980000257492065},{"id":"https://openalex.org/keywords/na-h-exchanger","display_name":"Na+/H+ exchanger","score":0.8759999871253967},{"id":"https://openalex.org/keywords/intracellular-ph","display_name":"intracellular pH","score":0.7409999966621399},{"id":"https://openalex.org/keywords/buffering-capacity","display_name":"buffering capacity","score":0.6690000295639038},{"id":"https://openalex.org/keywords/parathyroid-hormone","display_name":"parathyroid hormone","score":0.8460000157356262},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.375},{"id":"https://openalex.org/keywords/inulin-permeability","display_name":"inulin permeability","score":0.49000000953674316},{"id":"https://openalex.org/keywords/tubular-reabsorption","display_name":"tubular reabsorption","score":0.5920000076293945}],"concepts":[{"id":"https://openalex.org/C60903277","wikidata":"https://www.wikidata.org/wiki/Q2051926","display_name":"Paracellular transport","level":4,"score":0.6840527653694153},{"id":"https://openalex.org/C3018436504","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Apical membrane","level":3,"score":0.6505288481712341},{"id":"https://openalex.org/C56906281","wikidata":"https://www.wikidata.org/wiki/Q4391435","display_name":"Reabsorption","level":3,"score":0.5800911784172058},{"id":"https://openalex.org/C2779608910","wikidata":"https://www.wikidata.org/wiki/Q419995","display_name":"Amiloride","level":3,"score":0.5275280475616455},{"id":"https://openalex.org/C2779010597","wikidata":"https://www.wikidata.org/wiki/Q5166601","display_name":"Convoluted tubule","level":3,"score":0.48648354411125183},{"id":"https://openalex.org/C99975950","wikidata":"https://www.wikidata.org/wiki/Q911011","display_name":"Ion transporter","level":3,"score":0.48423218727111816},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.46411851048469543},{"id":"https://openalex.org/C188053792","wikidata":"https://www.wikidata.org/wiki/Q3640664","display_name":"Cotransporter","level":3,"score":0.46102744340896606},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43600189685821533},{"id":"https://openalex.org/C120405084","wikidata":"https://www.wikidata.org/wiki/Q285307","display_name":"Symporter","level":4,"score":0.4327056407928467},{"id":"https://openalex.org/C2778212192","wikidata":"https://www.wikidata.org/wiki/Q275926","display_name":"Bumetanide","level":4,"score":0.42680564522743225},{"id":"https://openalex.org/C2780498532","wikidata":"https://www.wikidata.org/wiki/Q7850941","display_name":"Tubule","level":3,"score":0.4140850901603699},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.41152098774909973},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3712439239025116},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3401612639427185},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2712530493736267},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.22280466556549072},{"id":"https://openalex.org/C2780091579","wikidata":"https://www.wikidata.org/wiki/Q9377","display_name":"Kidney","level":2,"score":0.21187064051628113},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.1535605788230896},{"id":"https://openalex.org/C120882062","wikidata":"https://www.wikidata.org/wiki/Q28352","display_name":"Permeability (electromagnetism)","level":3,"score":0.13130643963813782},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1111/j.1440-1797.1997.tb00213.x","doi":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1440-1797.1997.tb00213.x","pdf_url":null,"source":{"id":"https://openalex.org/S99619495","display_name":"Nephrology","issn_l":"1320-5358","issn":["1320-5358","1440-1797"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nephrology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","score":0.6499999761581421,"display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W21637438","https://openalex.org/W1481519187","https://openalex.org/W1565223184","https://openalex.org/W1928879395","https://openalex.org/W1967989557","https://openalex.org/W1974365751","https://openalex.org/W1974615597","https://openalex.org/W1983095757","https://openalex.org/W1987237681","https://openalex.org/W1996317129","https://openalex.org/W1997185330","https://openalex.org/W1999839467","https://openalex.org/W1999906008","https://openalex.org/W2000685512","https://openalex.org/W2025100148","https://openalex.org/W2026852431","https://openalex.org/W2029202642","https://openalex.org/W2030050746","https://openalex.org/W2078019864","https://openalex.org/W2078827911","https://openalex.org/W2086229992","https://openalex.org/W2095099518","https://openalex.org/W2122668255","https://openalex.org/W2235475950","https://openalex.org/W2249421789","https://openalex.org/W2271249968","https://openalex.org/W2342290122","https://openalex.org/W2403598783","https://openalex.org/W2405788503","https://openalex.org/W2410719978","https://openalex.org/W2412893553","https://openalex.org/W2415448705","https://openalex.org/W2415471485","https://openalex.org/W2415496155","https://openalex.org/W2417647235","https://openalex.org/W2426157868","https://openalex.org/W2464764495","https://openalex.org/W2578943258","https://openalex.org/W4238605098","https://openalex.org/W4245985386"],"related_works":["https://openalex.org/W65813198","https://openalex.org/W2443040314","https://openalex.org/W2247962593","https://openalex.org/W2412893553","https://openalex.org/W2149830185","https://openalex.org/W2101640127","https://openalex.org/W6884849621","https://openalex.org/W2057631242","https://openalex.org/W2085631433","https://openalex.org/W2171320890"],"abstract_inverted_index":{"Summary:In":[0],"order":[1],"to":[2,106,144],"establish":[3],"anin":[4],"vitromodel":[5],"for":[6,243,264],"studying":[7],"human":[8,15,65,69,147,212,240,274],"proximal":[9,16,275],"tubule":[10,17],"transport,":[11,161,165],"primary":[12],"culture":[13,244],"of":[14,30,40,50,54,64,110,151,197,211,254,267],"cells":[18,32],"(PTC)":[19],"was":[20,89,112,191,214,230],"carried":[21],"out":[22],"using":[23],"an":[24],"improved":[25],"technique":[26],"and":[27,45,58,74,82,132,170,182,202,218,250],"the":[28,107,140,206,219,236,255,265,273],"properties":[29,252],"these":[31],"were":[33,56,155,187],"characterised":[34],"in":[35,146,272],"detail.":[36],"Using":[37,175],"a":[38,225,261],"combination":[39],"collagenase":[41],"treatment,":[42],"mechanical":[43],"sieving":[44],"isopycnic":[46],"ultracentrifugation,":[47],"large":[48],"numbers":[49],"highly":[51,268],"purified":[52],"populations":[53],"PTC":[55,70,213],"isolated":[57],"propagated":[59],"from":[60],"histologically":[61],"normal":[62],"regions":[63],"nephrectomy":[66],"specimens.":[67],"Cultured":[68],"demonstrated":[71],"typical":[72],"histologic":[73],"ultrastructural":[75],"morphologies,":[76],"well\u2010preserved":[77],"brush":[78],"border":[79],"enzyme":[80],"activities,":[81],"cyclic":[83],"adenosine":[84],"monophosphate":[85],"(cAMP)":[86],"production":[87],"which":[88,136],"stimulated":[90],"by":[91,97,103,194],"parathyroid":[92],"hormone":[93],"(PTH)":[94],"but":[95],"not":[96],"vasopressin.":[98],"Tight":[99],"confluence,":[100],"as":[101,245],"evidenced":[102],"relative":[104],"impermeability":[105],"paracellular":[108],"diffusion":[109],"inulin,":[111],"achieved":[113],"on":[114],"porous":[115],"membrane":[116],"inserts":[117],"within":[118],"6\u20138":[119],"days.":[120],"Confluent":[121],"monolayers":[122],"generated":[123],"Na+,":[124],"K+,":[125],"Cl\u2212,":[126],"HCO3\u2212and":[127],"PO43\u2010concentration":[128],"gradients":[129],"between":[130],"apical":[131,159,163,181],"basolateral":[133,183],"medium":[134],"compartments,":[135],"correlated":[137],"well":[138],"with":[139],"reabsorption":[141],"processes":[142,271],"known":[143],"occur":[145],"PTCin":[148],"vivo.A":[149],"number":[150],"polarised":[152,269],"transport":[153,169,270],"systems":[154],"demonstrated,":[156],"including":[157],"phlorizin\u2010inhibitable":[158],"Na+\u2010glucose":[160],"PTH\u2010inhibitable":[162],"Na+\u2010phosphate":[164],"probenecid\u2010inhibitable":[166],"organic":[167,172],"anion":[168],"quinine\u2010inhibitable":[171],"cation":[173],"transport.":[174],"microspectrofluorimetric":[176],"and22Na+uptake":[177],"measurements,":[178],"pharmacologically":[179],"distinct":[180],"sodium\u2010hydrogen":[184],"exchangers":[185],"(NHE)":[186],"identified.":[188],"Apical":[189],"NHE":[190],"significantly":[192],"inhibited":[193],"micromolar":[195],"concentrations":[196],"phorbol":[198],"esters,":[199],"ethylisopropylamiloride":[200],"(EIPA)":[201],"3\u2010methylsulphonyl\u20104\u2010piperidino\u2010benzoylguanidine":[203],"methanesulphonate":[204],"(HOE694).":[205],"mean":[207,220],"resting":[208],"intracellular":[209],"pH":[210],"7.23":[215],"\u00b1":[216,232],"0.04":[217],"intrinsic":[221],"buffering":[222],"capacity":[223],"following":[224],"20":[226],"mmol/L":[227],"NH4Cl":[228],"prepulse":[229],"28.45":[231],"0.96":[233],"mmol/L/unit":[234],"pH.":[235],"results":[237],"suggest":[238],"that":[239],"PTC,":[241],"prepared":[242],"described":[246],"herein,":[247],"maintain":[248],"morphological":[249],"physiological":[251],"characteristic":[253],"segmentin":[256],"vivo.the":[257],"method":[258],"therefore":[259],"provides":[260],"useful":[262],"model":[263],"study":[266],"tubule.":[276]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2016-06-24T00:00:00"}
