{"id":"https://openalex.org/W2078636712","doi":"https://doi.org/10.1016/s0006-3495(92)81592-8","title":"Binding kinetics of an anti-dinitrophenyl monoclonal Fab on supported phospholipid monolayers measured by total internal reflection with fluorescence photobleaching recovery","display_name":"Binding kinetics of an anti-dinitrophenyl monoclonal Fab on supported phospholipid monolayers measured by total internal reflection with fluorescence photobleaching recovery","publication_year":1992,"publication_date":"1992-07-01","ids":{"openalex":"https://openalex.org/W2078636712","doi":"https://doi.org/10.1016/s0006-3495(92)81592-8","mag":"2078636712","pmid":"https://pubmed.ncbi.nlm.nih.gov/1420869","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1262139"},"language":"en","primary_location":{"id":"doi:10.1016/s0006-3495(92)81592-8","doi":"https://doi.org/10.1016/s0006-3495(92)81592-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","pdf_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","source":{"id":"https://openalex.org/S98085912","display_name":"Biophysical Journal","issn_l":"0006-3495","issn":["0006-3495","1542-0086"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biophysical Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024948191","display_name":"Mary Lee Pisarchick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M.L. Pisarchick","raw_affiliation_strings":["Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054229899","display_name":"D. Gesty","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. Gesty","raw_affiliation_strings":["Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113620785","display_name":"Nancy L. Thompson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N.L. Thompson","raw_affiliation_strings":["Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290","institution_ids":["https://openalex.org/I114027177"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I114027177"],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":1.1175,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.76520852,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"63","issue":"1","first_page":"215","last_page":"223"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.8169000148773193,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.8169000148773193,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.12559999525547028,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dissociation-kinetics","display_name":"dissociation kinetics","score":0.7179999947547913},{"id":"https://openalex.org/keywords/fab-fragment","display_name":"Fab fragment","score":0.9269999861717224},{"id":"https://openalex.org/keywords/frap-assay","display_name":"FRAP","score":0.8019999861717224},{"id":"https://openalex.org/keywords/total-internal-reflection","display_name":"total internal reflection","score":0.843999981880188},{"id":"https://openalex.org/keywords/dissociation-rate-constant","display_name":"dissociation rate constant","score":0.5230000019073486},{"id":"https://openalex.org/keywords/binding-constant","display_name":"binding constant","score":0.9110000133514404},{"id":"https://openalex.org/keywords/surface-diffusion","display_name":"surface diffusion","score":0.6880000233650208},{"id":"https://openalex.org/keywords/dipalmitoylphosphatidylethanolamine","display_name":"dipalmitoylphosphatidylethanolamine","score":0.8690000176429749},{"id":"https://openalex.org/keywords/dipalmitoylphosphatidylcholine","display_name":"DPPC","score":0.925000011920929},{"id":"https://openalex.org/keywords/antibody-antigen-binding","display_name":"antibody-antigen binding","score":0.5929999947547913}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8095967769622803},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.7919554710388184},{"id":"https://openalex.org/C2781172473","wikidata":"https://www.wikidata.org/wiki/Q2587934","display_name":"Dipalmitoylphosphatidylcholine","level":5,"score":0.7410901784896851},{"id":"https://openalex.org/C31332276","wikidata":"https://www.wikidata.org/wiki/Q899750","display_name":"Photobleaching","level":3,"score":0.7215598821640015},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6520752906799316},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.5992169380187988},{"id":"https://openalex.org/C121381206","wikidata":"https://www.wikidata.org/wiki/Q902514","display_name":"Total internal reflection fluorescence microscope","level":3,"score":0.5512465238571167},{"id":"https://openalex.org/C7070889","wikidata":"https://www.wikidata.org/wiki/Q902488","display_name":"Monolayer","level":2,"score":0.5358824133872986},{"id":"https://openalex.org/C93391505","wikidata":"https://www.wikidata.org/wiki/Q658700","display_name":"Reaction rate constant","level":3,"score":0.5212588906288147},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.5148202776908875},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.5064538717269897},{"id":"https://openalex.org/C2778177356","wikidata":"https://www.wikidata.org/wiki/Q19903955","display_name":"Dinitrophenyl","level":3,"score":0.45954009890556335},{"id":"https://openalex.org/C2994048982","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectrometry","level":3,"score":0.4437967538833618},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4001503884792328},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.1384841799736023},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08911368250846863},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.08802035450935364},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.06388214230537415},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C2776330855","wikidata":"https://www.wikidata.org/wiki/Q650187","display_name":"Phosphatidylcholine","level":4,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001703","descriptor_name":"Biophysics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004136","descriptor_name":"Dinitrobenzenes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007140","descriptor_name":"Immunoglobulin Fab Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008567","descriptor_name":"Membranes, Artificial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010743","descriptor_name":"Phospholipids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055592","descriptor_name":"Biophysical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0006-3495(92)81592-8","doi":"https://doi.org/10.1016/s0006-3495(92)81592-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","pdf_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","source":{"id":"https://openalex.org/S98085912","display_name":"Biophysical Journal","issn_l":"0006-3495","issn":["0006-3495","1542-0086"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biophysical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1262139","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1262139","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biophys J","raw_type":"Text"},{"id":"pmid:1420869","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1420869","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biophysical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0006-3495(92)81592-8","doi":"https://doi.org/10.1016/s0006-3495(92)81592-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","pdf_url":"https://doi.org/10.1016/s0006-3495(92)81592-8","source":{"id":"https://openalex.org/S98085912","display_name":"Biophysical Journal","issn_l":"0006-3495","issn":["0006-3495","1542-0086"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biophysical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2857939371","display_name":null,"funder_award_id":"GM-37145","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2078636712.pdf"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W1498664519","https://openalex.org/W1567249833","https://openalex.org/W1594409612","https://openalex.org/W1964638853","https://openalex.org/W1973599540","https://openalex.org/W1974378714","https://openalex.org/W1981121554","https://openalex.org/W2003747434","https://openalex.org/W2007173784","https://openalex.org/W2010515417","https://openalex.org/W2014944750","https://openalex.org/W2016818803","https://openalex.org/W2021472162","https://openalex.org/W2024914846","https://openalex.org/W2027308404","https://openalex.org/W2030386167","https://openalex.org/W2037866847","https://openalex.org/W2038183356","https://openalex.org/W2043367471","https://openalex.org/W2044231254","https://openalex.org/W2044380749","https://openalex.org/W2048160836","https://openalex.org/W2048372210","https://openalex.org/W2050872000","https://openalex.org/W2056847422","https://openalex.org/W2062721493","https://openalex.org/W2064333737","https://openalex.org/W2064578334","https://openalex.org/W2070210111","https://openalex.org/W2079632351","https://openalex.org/W2083840888","https://openalex.org/W2096963388","https://openalex.org/W2115150990","https://openalex.org/W2117662282","https://openalex.org/W2409745684"],"related_works":["https://openalex.org/W2072935643","https://openalex.org/W1993308893","https://openalex.org/W2016818803","https://openalex.org/W97848455","https://openalex.org/W2050872000","https://openalex.org/W428278000","https://openalex.org/W2030386167","https://openalex.org/W2079324740","https://openalex.org/W2022831697","https://openalex.org/W2063949548"],"abstract_inverted_index":null,"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}