{"id":"https://openalex.org/W2078066804","doi":"https://doi.org/10.1021/bi100502z","title":"Kinetic and Structural Characterization of a Heterohexamer 4-Oxalocrotonate Tautomerase from Chloroflexus aurantiacus J-10-fl: Implications for Functional and Structural Diversity in the Tautomerase Superfamily,","display_name":"Kinetic and Structural Characterization of a Heterohexamer 4-Oxalocrotonate Tautomerase from Chloroflexus aurantiacus J-10-fl: Implications for Functional and Structural Diversity in the Tautomerase Superfamily,","publication_year":2010,"publication_date":"2010-05-13","ids":{"openalex":"https://openalex.org/W2078066804","doi":"https://doi.org/10.1021/bi100502z","mag":"2078066804","pmid":"https://pubmed.ncbi.nlm.nih.gov/20465238","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2913408"},"language":"en","primary_location":{"id":"doi:10.1021/bi100502z","doi":"https://doi.org/10.1021/bi100502z","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi100502z","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2913408","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005857606","display_name":"Elizabeth A. Burks","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elizabeth A. Burks","raw_affiliation_strings":["Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712","Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712 USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039578582","display_name":"Christopher D. Fleming","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher D. Fleming","raw_affiliation_strings":["Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010165126","display_name":"Andrew D. Mesecar","orcid":"https://orcid.org/0000-0002-1241-2577","observed_orcids":["https://orcid.org/0000-0002-1241-2577"]},"institutions":[{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew D. Mesecar","raw_affiliation_strings":["Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173","institution_ids":["https://openalex.org/I39422238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084564457","display_name":"Christian P. Whitman","orcid":"https://orcid.org/0000-0002-8231-2483","observed_orcids":["https://orcid.org/0000-0002-8231-2483"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christian P. Whitman","raw_affiliation_strings":["Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, The University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047964465","display_name":"Scott D. Pegan","orcid":"https://orcid.org/0000-0002-2958-5319","observed_orcids":["https://orcid.org/0000-0002-2958-5319"]},"institutions":[{"id":"https://openalex.org/I131651094","display_name":"University of Denver","ror":"https://ror.org/04w7skc03","country_code":"US","type":"education","lineage":["https://openalex.org/I131651094"]},{"id":"https://openalex.org/I39422238","display_name":"University of Illinois Chicago","ror":"https://ror.org/02mpq6x41","country_code":"US","type":"education","lineage":["https://openalex.org/I39422238"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Scott D. Pegan","raw_affiliation_strings":["Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173","Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208-0183"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Pharmaceutical Biotechnology (MC 870), College of Pharmacy, University of Illinois, 900 South Ashland Avenue, Chicago, Illinois 60607-7173","institution_ids":["https://openalex.org/I39422238"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208-0183","institution_ids":["https://openalex.org/I131651094"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5047964465","https://openalex.org/A5084564457"],"corresponding_institution_ids":["https://openalex.org/I131651094","https://openalex.org/I39422238","https://openalex.org/I86519309"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.5692,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.82544223,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"49","issue":"24","first_page":"5016","last_page":"5027"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/4-oxalocrotonate-tautomerase","display_name":"4-oxalocrotonate tautomerase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/heterohexamer","display_name":"heterohexamer","score":0.8799999952316284},{"id":"https://openalex.org/keywords/chloroflexus-aurantiacus","display_name":"Chloroflexus aurantiacus","score":0.9570000171661377},{"id":"https://openalex.org/keywords/aromatic-hydrocarbon-degradation","display_name":"aromatic hydrocarbon degradation","score":0.6309999823570251},{"id":"https://openalex.org/keywords/thermophilic-bacteria","display_name":"thermophilic bacteria","score":0.703000009059906},{"id":"https://openalex.org/keywords/computer-aided-architectural-design","display_name":"computer-aided architectural design","score":0.6200000047683716},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5149999856948853},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.6439999938011169},{"id":"https://openalex.org/keywords/pseudomonas-putida-mt-2","display_name":"Pseudomonas putida mt-2","score":0.7910000085830688}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7387387752532959},{"id":"https://openalex.org/C2780543182","wikidata":"https://www.wikidata.org/wiki/Q2738168","display_name":"Pseudomonas putida","level":3,"score":0.5995819568634033},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5694423317909241},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5627355575561523},{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.560304582118988},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5242695808410645},{"id":"https://openalex.org/C45484198","wikidata":"https://www.wikidata.org/wiki/Q827246","display_name":"Sequence alignment","level":4,"score":0.4729052782058716},{"id":"https://openalex.org/C199216141","wikidata":"https://www.wikidata.org/wiki/Q4995178","display_name":"Conserved sequence","level":4,"score":0.4496201276779175},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4395958185195923},{"id":"https://openalex.org/C178180057","wikidata":"https://www.wikidata.org/wiki/Q7251477","display_name":"Protein superfamily","level":3,"score":0.4364357888698578},{"id":"https://openalex.org/C550995028","wikidata":"https://www.wikidata.org/wiki/Q10872","display_name":"Archaea","level":3,"score":0.4295973777770996},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.4217369556427002},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32449519634246826},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2178695797920227}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007535","descriptor_name":"Isomerases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007535","descriptor_name":"Isomerases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D041861","descriptor_name":"Chloroflexus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055503","descriptor_name":"Protein Multimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1021/bi100502z","doi":"https://doi.org/10.1021/bi100502z","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi100502z","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:20465238","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20465238","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:2118575","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2913408","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:2913408","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2913408","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Biochemistry","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2118575","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2913408","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1061224980","display_name":"Novel Protease Inhibitors as SARS Therapeutics","funder_award_id":"P01AI060915","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G1592235300","display_name":null,"funder_award_id":"#ES07784","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G4205975939","display_name":null,"funder_award_id":"GM-41239","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5484512101","display_name":"Mechanisms and Prevention of Environmental Disease","funder_award_id":"P30ES007784","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G7322924398","display_name":null,"funder_award_id":"AI-60915","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G908848628","display_name":"Enzymes of the Meta Fission Pathway","funder_award_id":"R01GM041239","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W1525251521","https://openalex.org/W1539796472","https://openalex.org/W1557707972","https://openalex.org/W1588665944","https://openalex.org/W1966735171","https://openalex.org/W1967712722","https://openalex.org/W1969466983","https://openalex.org/W1969518814","https://openalex.org/W1972722145","https://openalex.org/W1996798502","https://openalex.org/W1997293305","https://openalex.org/W1999767410","https://openalex.org/W2000297152","https://openalex.org/W2001641653","https://openalex.org/W2008708467","https://openalex.org/W2008921808","https://openalex.org/W2011685425","https://openalex.org/W2018012868","https://openalex.org/W2035156848","https://openalex.org/W2045017771","https://openalex.org/W2046048339","https://openalex.org/W2048783098","https://openalex.org/W2056296814","https://openalex.org/W2058852594","https://openalex.org/W2064926383","https://openalex.org/W2070568587","https://openalex.org/W2076412028","https://openalex.org/W2086700751","https://openalex.org/W2094342959","https://openalex.org/W2100837269","https://openalex.org/W2104176629","https://openalex.org/W2104215735","https://openalex.org/W2139217585","https://openalex.org/W2143348837","https://openalex.org/W2144081223","https://openalex.org/W2144634347","https://openalex.org/W2149480059","https://openalex.org/W2149653798","https://openalex.org/W2155257397","https://openalex.org/W2299038488","https://openalex.org/W2314225431","https://openalex.org/W3169629205","https://openalex.org/W4249170421"],"related_works":["https://openalex.org/W2048783098","https://openalex.org/W2944232128","https://openalex.org/W2056296814","https://openalex.org/W2125097802","https://openalex.org/W2766615883","https://openalex.org/W2091465930","https://openalex.org/W2037581433","https://openalex.org/W2087690058","https://openalex.org/W1966735171","https://openalex.org/W2077680621"],"abstract_inverted_index":{"4-Oxalocrotonate":[0],"tautomerase":[1,74,199],"(4-OT)":[2],"isozymes":[3],"play":[4],"prominent":[5],"roles":[6],"in":[7,89,246],"the":[8,21,41,45,48,52,62,69,73,109,133,146,162,168,175,180,194,202,215,219,222,238,242],"bacterial":[9,81],"utilization":[10],"of":[11,23,44,51,72,78,122,157,177,197,209,214,241],"aromatic":[12],"hydrocarbons":[13],"as":[14,32,95,114],"sole":[15],"carbon":[16],"sources.":[17],"These":[18],"enzymes":[19],"catalyze":[20],"conversion":[22,176],"2-hydroxy-2,4-hexadienedioate":[24],"(or":[25],"2-hydroxymuconate)":[26],"to":[27,47,179,188],"2-oxo-3-hexenedioate,":[28],"where":[29],"Pro-1":[30],"functions":[31],"a":[33,38,55,84,96,103,115,151],"general":[34],"base":[35],"and":[36,68,132,140,171,233],"shuttles":[37],"proton":[39],"from":[40,57,126],"2-hydroxyl":[42],"group":[43,121],"substrate":[46],"C-5":[49],"position":[50],"product.":[53],"4-OT,":[54,189],"homohexamer":[56],"Pseudomonas":[58],"putida":[59,164],"mt-2,":[60],"is":[61,111],"most":[63],"extensively":[64],"studied":[65],"4-OT":[66,154],"isozyme":[67],"founding":[70],"member":[71],"superfamily.":[75],"A":[76],"search":[77],"five":[79],"thermophilic":[80],"genomes":[82],"identified":[83],"coded":[85],"amino":[86],"acid":[87,205],"sequence":[88,105,110],"each":[90],"that":[91,145],"had":[92],"been":[93],"annotated":[94,113],"tautomerase-like":[97,116],"protein":[98],"but":[99,108],"lacked":[100],"Pro-1.":[101],"However,":[102],"nearby":[104],"has":[106],"Pro-1,":[107],"not":[112,192],"protein.":[117],"To":[118],"characterize":[119],"this":[120],"proteins,":[123],"two":[124,147,172],"genes":[125],"Chloroflexus":[127],"aurantiacus":[128],"J-10-fl":[129],"were":[130,136],"cloned,":[131],"corresponding":[134],"proteins":[135,149],"expressed.":[137],"Kinetic,":[138],"biochemical,":[139],"X-ray":[141],"structural":[142],"analyses":[143],"show":[144],"expressed":[148],"form":[150],"functional":[152,212],"heterohexamer":[153,203],"(hh4-OT),":[155],"composed":[156],"three":[158],"alphabeta":[159],"dimers.":[160],"Like":[161],"P.":[163],"enzyme,":[165,232],"hh4-OT":[166,190,210],"requires":[167],"amino-terminal":[169],"proline":[170],"arginines":[173],"for":[174],"2-hydroxymuconate":[178],"product,":[181],"implicating":[182],"an":[183,230],"analogous":[184],"mechanism.":[185],"In":[186],"contrast":[187],"does":[191],"exhibit":[193],"low-level":[195,243],"activity":[196,245],"another":[198],"superfamily":[200],"member,":[201],"trans-3-chloroacrylic":[204],"dehalogenase":[206],"(CaaD).":[207],"Characterization":[208],"enables":[211],"assignment":[213],"related":[216],"enzymes,":[217],"highlights":[218],"diverse":[220],"ways":[221],"beta-alpha-beta":[223],"building":[224],"block":[225],"can":[226],"be":[227],"assembled":[228],"into":[229,237],"active":[231],"provides":[234],"further":[235],"insight":[236],"molecular":[239],"basis":[240],"CaaD":[244],"4-OT.":[247]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}