{"id":"https://openalex.org/W2077595754","doi":"https://doi.org/10.1074/jbc.273.14.8094","title":"Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I)","display_name":"Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I)","publication_year":1998,"publication_date":"1998-04-01","ids":{"openalex":"https://openalex.org/W2077595754","doi":"https://doi.org/10.1074/jbc.273.14.8094","mag":"2077595754","pmid":"https://pubmed.ncbi.nlm.nih.gov/9525911"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.273.14.8094","doi":"https://doi.org/10.1074/jbc.273.14.8094","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.14.8094","pdf_url":"http://www.jbc.org/article/S0021925818525321/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925818525321/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022757762","display_name":"Mutsuo Yamaguchi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mutsuo Yamaguchi","raw_affiliation_strings":["Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA","From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089201329","display_name":"Grigory I. Belogrudov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grigory I. Belogrudov","raw_affiliation_strings":["Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037","From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088361501","display_name":"Youssef Hatefi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Youssef Hatefi","raw_affiliation_strings":["Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037","From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"From the Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La\u2009Jolla,\u2009California\u200992037","institution_ids":["https://openalex.org/I123431417"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088361501"],"corresponding_institution_ids":["https://openalex.org/I123431417"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.3946,"has_fulltext":true,"cited_by_count":65,"citation_normalized_percentile":{"value":0.80802959,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"273","issue":"14","first_page":"8094","last_page":"8098"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.8550000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.8550000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.10559999942779541,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13012","display_name":"Coenzyme Q10 studies and effects","score":0.010900000110268593,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mitochondrial-complex-i","display_name":"mitochondrial complex I","score":0.9670000076293945},{"id":"https://openalex.org/keywords/complex-i","display_name":"complex I","score":0.996999979019165},{"id":"https://openalex.org/keywords/nadph","display_name":"NADPH","score":0.6610000133514404},{"id":"https://openalex.org/keywords/nadh","display_name":"NADH","score":0.9039999842643738},{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.7049999833106995},{"id":"https://openalex.org/keywords/flavin-mononucleotide","display_name":"flavin mononucleotide","score":0.7429999709129333},{"id":"https://openalex.org/keywords/iron-sulfur-clusters","display_name":"iron-sulfur clusters","score":0.9990000128746033},{"id":"https://openalex.org/keywords/nadp-binding","display_name":"NADP+ binding","score":0.7620000243186951},{"id":"https://openalex.org/keywords/acyl-carrier-protein","display_name":"acyl carrier protein","score":0.7829999923706055},{"id":"https://openalex.org/keywords/electron-transfer","display_name":"electron transfer","score":0.6610000133514404},{"id":"https://openalex.org/keywords/energy-coupling","display_name":"energy coupling","score":0.6819999814033508},{"id":"https://openalex.org/keywords/bovine-mitochondria","display_name":"bovine mitochondria","score":0.6869999766349792}],"concepts":[{"id":"https://openalex.org/C60538801","wikidata":"https://www.wikidata.org/wiki/Q7631706","display_name":"Submitochondrial particle","level":3,"score":0.8096864223480225},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.7791979312896729},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.6466408967971802},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.622879683971405},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5651825070381165},{"id":"https://openalex.org/C2776319529","wikidata":"https://www.wikidata.org/wiki/Q3869349","display_name":"NADH dehydrogenase","level":4,"score":0.5440762042999268},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4709022343158722},{"id":"https://openalex.org/C2909692557","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Electron Transport Complex I","level":4,"score":0.4568929672241211},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.45039528608322144},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.43026238679885864},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.358154833316803},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008929","descriptor_name":"Mitochondria, Heart","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016660","descriptor_name":"NAD(P)H Dehydrogenase (Quinone)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.273.14.8094","doi":"https://doi.org/10.1074/jbc.273.14.8094","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.14.8094","pdf_url":"http://www.jbc.org/article/S0021925818525321/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9525911","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9525911","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.273.14.8094","doi":"https://doi.org/10.1074/jbc.273.14.8094","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.14.8094","pdf_url":"http://www.jbc.org/article/S0021925818525321/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5641894249","display_name":null,"funder_award_id":"DK-08126","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2077595754.pdf","grobid_xml":"https://content.openalex.org/works/W2077595754.grobid-xml"},"referenced_works_count":58,"referenced_works":["https://openalex.org/W134719735","https://openalex.org/W427869391","https://openalex.org/W1026284996","https://openalex.org/W1191980701","https://openalex.org/W1506043065","https://openalex.org/W1528193170","https://openalex.org/W1539603839","https://openalex.org/W1557704072","https://openalex.org/W1576822037","https://openalex.org/W1767553051","https://openalex.org/W1787223099","https://openalex.org/W1932052686","https://openalex.org/W1964976203","https://openalex.org/W1967750933","https://openalex.org/W1970231327","https://openalex.org/W1971026792","https://openalex.org/W1976831120","https://openalex.org/W1979368553","https://openalex.org/W1991185618","https://openalex.org/W1991205189","https://openalex.org/W1991227065","https://openalex.org/W1991710978","https://openalex.org/W1999118816","https://openalex.org/W1999169034","https://openalex.org/W2001680212","https://openalex.org/W2012003686","https://openalex.org/W2019280050","https://openalex.org/W2020969870","https://openalex.org/W2025590129","https://openalex.org/W2026681275","https://openalex.org/W2030963789","https://openalex.org/W2036607640","https://openalex.org/W2039280872","https://openalex.org/W2039559630","https://openalex.org/W2041850796","https://openalex.org/W2047687152","https://openalex.org/W2050394208","https://openalex.org/W2058287540","https://openalex.org/W2067220884","https://openalex.org/W2068276476","https://openalex.org/W2070641835","https://openalex.org/W2076971346","https://openalex.org/W2077170431","https://openalex.org/W2087524379","https://openalex.org/W2091381615","https://openalex.org/W2091696298","https://openalex.org/W2092955506","https://openalex.org/W2100837269","https://openalex.org/W2102131116","https://openalex.org/W2107969909","https://openalex.org/W2128171938","https://openalex.org/W2133205270","https://openalex.org/W2134153749","https://openalex.org/W2165268250","https://openalex.org/W2200032390","https://openalex.org/W2521919530","https://openalex.org/W4231506321","https://openalex.org/W4237825483"],"related_works":["https://openalex.org/W2086111188","https://openalex.org/W2091696298","https://openalex.org/W1565448991","https://openalex.org/W2002586559","https://openalex.org/W71777235","https://openalex.org/W2161816721","https://openalex.org/W1554104048","https://openalex.org/W1986003680","https://openalex.org/W1788512958","https://openalex.org/W2066736258"],"abstract_inverted_index":{"It":[0,213],"has":[1,157],"been":[2,158],"shown":[3,58,105,159],"that":[4,39,53,90,106,139,161,187,216],"treatment":[5,162],"of":[6,26,29,66,112,163,171,223],"bovine":[7],"mitochondrial":[8],"complex":[9,67,91,113,143,164,224],"I":[10,68,92,114,144,165,225],"(NADH-ubiquinone":[11],"oxidoreductase)":[12],"with":[13,19,75,166,208,218],"NADH":[14,76,175],"or":[15,21,77,176],"NADPH,":[16,172],"but":[17,173],"not":[18,174],"NAD":[20,116,126],"NADP,":[22],"increases":[23,117],"the":[24,63,71,110,128,169,180,192,195,219,226],"susceptibility":[25,36],"a":[27,151,183,200,209],"number":[28],"subunits":[30,38,52,69],"to":[31,125,199],"tryptic":[32],"degradation.":[33],"This":[34],"increased":[35],"involved":[37,231],"contain":[40],"electron":[41,55,121],"carriers,":[42],"such":[43],"as":[44,49,51,150],"FMN":[45],"and":[46,81,204],"iron-sulfur":[47],"clusters,":[48],"well":[50],"lack":[54],"carriers.":[56],"Results":[57],"elsewhere":[59],"on":[60],"changes":[61,96,149],"in":[62,107,120,142,168,232],"cross-linking":[64],"pattern":[65],"when":[70,97,127],"enzyme":[72],"was":[73],"pretreated":[74],"NADPH":[78],"(Belogrudov,":[79],"G.,":[80],"Hatefi,":[82],"Y.":[83],"(1994)":[84],"Biochemistry":[85],"33,":[86],"4571-4576)":[87],"also":[88],"indicated":[89],"undergoes":[93],"extensive":[94],"conformation":[95,148],"reduced":[98],"by":[99,118,132],"substrate.":[100],"Furthermore,":[101],"we":[102],"had":[103],"previously":[104],"submitochondrial":[108],"particles":[109,129],"affinity":[111,202],"for":[115],">/=20-fold":[119],"transfer":[122],"from":[123,179],"succinate":[124],"are":[130],"energized":[131],"ATP":[133],"hydrolysis.":[134,190],"Together,":[135],"these":[136],"results":[137],"suggest":[138],"energy":[140],"coupling":[141],"may":[145],"involve":[146],"protein":[147,222],"key":[152],"step.":[153],"In":[154],"addition,":[155],"it":[156],"here":[160],"trypsin":[167],"presence":[170],"NAD(P),":[177],"produced":[178],"39-kDa":[181,193,228],"subunit":[182,229],"33-kDa":[184,196],"degradation":[185],"product":[186,197],"resisted":[188],"further":[189],"Like":[191],"subunit,":[194],"bound":[198],"NADP-agarose":[201],"column,":[203],"could":[205],"be":[206],"eluted":[207],"buffer":[210],"containing":[211],"NADPH.":[212],"is":[214,230],"possible":[215],"together":[217],"acyl":[220],"carrier":[221],"NADP(H)-binding":[227],"intramitochondrial":[233],"fatty":[234],"acid":[235],"synthesis.":[236]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
