{"id":"https://openalex.org/W2077532589","doi":"https://doi.org/10.1021/bi00379a009","title":"Subunit interface of triosephosphate isomerase: site-directed mutagenesis and characterization of the altered enzyme","display_name":"Subunit interface of triosephosphate isomerase: site-directed mutagenesis and characterization of the altered enzyme","publication_year":1987,"publication_date":"1987-03-01","ids":{"openalex":"https://openalex.org/W2077532589","doi":"https://doi.org/10.1021/bi00379a009","mag":"2077532589","pmid":"https://pubmed.ncbi.nlm.nih.gov/3552044"},"language":"en","primary_location":{"id":"doi:10.1021/bi00379a009","doi":"https://doi.org/10.1021/bi00379a009","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00379a009","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080722826","display_name":"Jos\u00e9 Ignacio Casal","orcid":"https://orcid.org/0000-0003-1085-2840","observed_orcids":["https://orcid.org/0000-0003-1085-2840"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jose I. Casal","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016302221","display_name":"Tim J. Ahern","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tim J. Ahern","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045614025","display_name":"R. Davenport","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Robert C. Davenport","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007934367","display_name":"Gregory A. Petsko","orcid":"https://orcid.org/0000-0003-3668-3694","observed_orcids":["https://orcid.org/0000-0003-3668-3694"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gregory A. Petsko","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5023002381","display_name":"Alexander M. Klibanov","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alexander M. Klibanov","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.2779,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.79670113,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"26","issue":"5","first_page":"1258","last_page":"1264"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.8295999765396118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.8295999765396118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.12950000166893005,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.015399999916553497,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/triosephosphate-isomerase","display_name":"triosephosphate isomerase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/subunit-interface","display_name":"subunit interface","score":0.9599999785423279},{"id":"https://openalex.org/keywords/asparagine-substitution","display_name":"asparagine substitution","score":0.6880000233650208},{"id":"https://openalex.org/keywords/catalytic-efficiency","display_name":"catalytic efficiency","score":0.6830000281333923},{"id":"https://openalex.org/keywords/d-glyceraldehyde-3-phosphate","display_name":"D-glyceraldehyde-3-phosphate","score":0.8740000128746033},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.5720000267028809},{"id":"https://openalex.org/keywords/deamidation","display_name":"deamidation","score":0.7009999752044678},{"id":"https://openalex.org/keywords/isoelectric-point","display_name":"isoelectric point","score":0.8740000128746033},{"id":"https://openalex.org/keywords/protein-denaturation","display_name":"protein denaturation","score":0.656000018119812},{"id":"https://openalex.org/keywords/protease-resistance","display_name":"protease resistance","score":0.6420000195503235},{"id":"https://openalex.org/keywords/yeast-enzymes","display_name":"yeast enzymes","score":0.49000000953674316}],"concepts":[{"id":"https://openalex.org/C2778727959","wikidata":"https://www.wikidata.org/wiki/Q24777683","display_name":"Triosephosphate isomerase","level":3,"score":0.9766616821289062},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6366285085678101},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5751208066940308},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5694049596786499},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5433512330055237},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5100961327552795},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4845496714115143},{"id":"https://openalex.org/C2781264208","wikidata":"https://www.wikidata.org/wiki/Q118026","display_name":"Isomerase","level":3,"score":0.43054595589637756},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3462677597999573},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.16994884610176086},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.07753878831863403},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002238","descriptor_name":"Carbohydrate Epimerases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007158","descriptor_name":"Immunologic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007526","descriptor_name":"Isoelectric Point","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014305","descriptor_name":"Triose-Phosphate Isomerase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014305","descriptor_name":"Triose-Phosphate Isomerase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D014305","descriptor_name":"Triose-Phosphate Isomerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00379a009","doi":"https://doi.org/10.1021/bi00379a009","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00379a009","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:3552044","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3552044","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7409786955","display_name":"Pre-Doctoral Training in Biological Sciences","funder_award_id":"T32GM007287","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W89774207","https://openalex.org/W1498479340","https://openalex.org/W1546044265","https://openalex.org/W1548262973","https://openalex.org/W1558516755","https://openalex.org/W1571793851","https://openalex.org/W1590376353","https://openalex.org/W1611099377","https://openalex.org/W1903489927","https://openalex.org/W1964449641","https://openalex.org/W1974873606","https://openalex.org/W1985702277","https://openalex.org/W1993606408","https://openalex.org/W1998265315","https://openalex.org/W2004773817","https://openalex.org/W2011235810","https://openalex.org/W2016586557","https://openalex.org/W2019410656","https://openalex.org/W2026530009","https://openalex.org/W2030456541","https://openalex.org/W2031335201","https://openalex.org/W2040351161","https://openalex.org/W2041453254","https://openalex.org/W2044952215","https://openalex.org/W2070214907","https://openalex.org/W2073729684","https://openalex.org/W2080779467","https://openalex.org/W2095237995","https://openalex.org/W2100837269","https://openalex.org/W2138270253","https://openalex.org/W2173668937","https://openalex.org/W2306066944","https://openalex.org/W2417661640"],"related_works":["https://openalex.org/W2085162051","https://openalex.org/W2161804198","https://openalex.org/W2022132907","https://openalex.org/W2003960201","https://openalex.org/W2023688586","https://openalex.org/W2278176906","https://openalex.org/W2006380640","https://openalex.org/W2079280317","https://openalex.org/W2167955016","https://openalex.org/W1971026909"],"abstract_inverted_index":{"We":[0],"have":[1],"replaced":[2],"asparagine":[3],"residues":[4,66],"at":[5],"the":[6,21,26,33,47,71,76,81,89,97],"subunit":[7],"interface":[8],"of":[9,23,32,43,59,96,104],"yeast":[10,106],"triosephosphate":[11],"isomerase":[12,49],"(TIM)":[13],"using":[14],"site-directed":[15],"mutagenesis":[16],"in":[17,40],"order":[18],"to":[19,102,125],"elucidate":[20],"effects":[22],"substitutions":[24],"on":[25,70],"catalytic":[27,73],"activity":[28],"and":[29,50,54,80,115,127,138],"conformational":[30],"stability":[31],"enzyme.":[34],"The":[35,93],"mutant":[36,98,117],"proteins":[37],"were":[38],"expressed":[39],"a":[41,111],"strain":[42],"Escherichia":[44],"coli":[45],"lacking":[46],"bacterial":[48],"purified":[51],"by":[52,61,130],"ion-exchange":[53],"immunoadsorption":[55],"chromatography.":[56],"Single":[57],"replacements":[58],"Asn-78":[60],"either":[62],"Thr":[63],"or":[64],"Ile":[65],"had":[67,109],"little":[68],"effect":[69],"enzyme's":[72],"efficiency,":[74],"while":[75],"single":[77],"replacement":[78,83],"Asn-78----Asp-78":[79,99],"double":[82],"Asn-14/Asn-78----Thr-14/Ile-78":[84],"appreciably":[85],"lowered":[86],"kcat":[87],"for":[88],"substrate":[90],"D-glyceraldehyde":[91],"3-phosphate.":[92],"isoelectric":[94],"point":[95],"was":[100,119],"equivalent":[101],"that":[103,108],"wild-type":[105,123],"TIM":[107,124],"undergone":[110],"single,":[112],"heat-induced":[113],"deamidation,":[114],"this":[116],"enzyme":[118],"less":[120],"resistant":[121],"than":[122],"denaturation":[126],"inactivation":[128],"caused":[129],"elevated":[131],"temperature,":[132],"denaturants,":[133],"tetrabutylammonium":[134],"bromide,":[135],"alkaline":[136],"pH,":[137],"proteases.":[139]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}
