{"id":"https://openalex.org/W2076970771","doi":"https://doi.org/10.1021/bi9609937","title":"Structural Determinants of Nucleotide Coenzyme Specificity in the Distinctive Dinucleotide Binding Fold of HMG-CoA Reductase from Pseudomonas mevalonii","display_name":"Structural Determinants of Nucleotide Coenzyme Specificity in the Distinctive Dinucleotide Binding Fold of HMG-CoA Reductase from Pseudomonas mevalonii","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2076970771","doi":"https://doi.org/10.1021/bi9609937","mag":"2076970771","pmid":"https://pubmed.ncbi.nlm.nih.gov/8810898"},"language":"en","primary_location":{"id":"doi:10.1021/bi9609937","doi":"https://doi.org/10.1021/bi9609937","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi9609937","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023185733","display_name":"Jon Alan Friesen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jon A. Friesen","raw_affiliation_strings":["Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051362535","display_name":"C. Martin Lawrence","orcid":"https://orcid.org/0000-0002-5398-466X","observed_orcids":["https://orcid.org/0000-0002-5398-466X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Martin Lawrence","raw_affiliation_strings":["Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031822398","display_name":"Cynthia V. Stauffacher","orcid":"https://orcid.org/0000-0002-3081-6903","observed_orcids":["https://orcid.org/0000-0002-3081-6903"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cynthia V. Stauffacher","raw_affiliation_strings":["Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004103648","display_name":"Victor W. Rodwell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Victor W. Rodwell","raw_affiliation_strings":["Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Biological Sciences, Purdue University, West Lafayette, Indiana 47907","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.2655,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.79026846,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"35","issue":"37","first_page":"11945","last_page":"11950"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.049800001084804535,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hmg-coa-reductase","display_name":"HMG-CoA reductase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/nadp-specificity","display_name":"NADP+ specificity","score":0.800000011920929},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7639999985694885},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.49799999594688416},{"id":"https://openalex.org/keywords/coenzyme-specificity","display_name":"coenzyme specificity","score":0.550000011920929},{"id":"https://openalex.org/keywords/protein-structure-function-relationship","display_name":"protein structure-function relationship","score":0.3370000123977661}],"concepts":[{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.6135407090187073},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5961822271347046},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.5691965818405151},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5294812917709351},{"id":"https://openalex.org/C104950815","wikidata":"https://www.wikidata.org/wiki/Q415607","display_name":"HMG-CoA reductase","level":4,"score":0.5172958374023438},{"id":"https://openalex.org/C134651460","wikidata":"https://www.wikidata.org/wiki/Q3100717","display_name":"Reductase","level":3,"score":0.49027541279792786},{"id":"https://openalex.org/C2779123261","wikidata":"https://www.wikidata.org/wiki/Q407635","display_name":"Coenzyme A","level":4,"score":0.4592679738998413},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3362399637699127},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07215261459350586}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006903","descriptor_name":"Hydroxymethylglutaryl CoA Reductases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006903","descriptor_name":"Hydroxymethylglutaryl CoA Reductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006903","descriptor_name":"Hydroxymethylglutaryl CoA Reductases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012617","descriptor_name":"Sea Urchins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017354","descriptor_name":"Point Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi9609937","doi":"https://doi.org/10.1021/bi9609937","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi9609937","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:8810898","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8810898","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6206570584","display_name":"CATALYSIS AND PHOSPHORYLATION OF HMG-COA REDUCTASE","funder_award_id":"R01HL047113","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G7605115886","display_name":"MOLECULAR MECHANISM OF HMG COA REDUCTASE","funder_award_id":"R01HL052115","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W628555867","https://openalex.org/W1520961658","https://openalex.org/W1564756056","https://openalex.org/W1676484817","https://openalex.org/W1967644006","https://openalex.org/W1999224568","https://openalex.org/W2020145731","https://openalex.org/W2077818961","https://openalex.org/W2100498403","https://openalex.org/W2132828385","https://openalex.org/W2157281491","https://openalex.org/W2314225431","https://openalex.org/W4245121165"],"related_works":["https://openalex.org/W6996110316","https://openalex.org/W2104541804","https://openalex.org/W2072106016","https://openalex.org/W1998333865","https://openalex.org/W2773975952","https://openalex.org/W2073404777","https://openalex.org/W2143581822","https://openalex.org/W4225399323","https://openalex.org/W7043534362","https://openalex.org/W2060049336"],"abstract_inverted_index":{"The":[0,24,58],"102-residue":[1],"small":[2],"domain":[3],"of":[4,10,35,41,46,71,87,102,118,154,170],"the":[5,33,39,68,77,82,94,100,168,188,194],"428-residue":[6],"NAD(H)-dependent":[7],"HMG-CoA":[8,74],"reductase":[9],"Pseudomonas":[11],"mevalonii":[12],"(EC":[13],"1.1.1.88)":[14],"binds":[15],"NAD(H)":[16],"at":[17],"a":[18],"distinctive,":[19],"non-Rossmann":[20],"dinucleotide":[21],"binding":[22],"fold.":[23],"three-dimensional":[25],"structure":[26],"reveals":[27],"that":[28],"Asp146":[29,49,88,119],"lies":[30],"close":[31],"to":[32,52,66,73,89,106,193],"2'-OH":[34],"NAD-.":[36],"To":[37,144],"investigate":[38],"role":[40],"this":[42],"residue":[43],"in":[44,129,140,187],"determination":[45],"coenzyme":[47,79,84],"specificity,":[48,148],"was":[50,120,159],"mutated":[51],"Ala,":[53],"Gly,":[54],"Ser,":[55],"and":[56,113,125,133,136,177,184],"Asn.":[57],"mutant":[59],"enzymes":[60],"were":[61],"analyzed":[62],"for":[63,96,104,108,131,142,190],"their":[64],"ability":[65],"catalyze":[67],"oxidative":[69],"acylation":[70],"mevalonate":[72],"using":[75],"either":[76],"natural":[78],"NAD+":[80],"or":[81,91,152,157,163],"alternate":[83],"NADP+.":[85,171],"Mutation":[86,117],"Ala":[90],"Gly":[92],"increased":[93],"specificity":[95,189],"NADP+,":[97],"expressed":[98],"as":[99],"ratio":[101],"kcat/K(m)":[103,107,130,141],"NADP+":[105,132,147,191],"NAD+,":[109],"1200-fold":[110],"(enzyme":[111,115],"D146G)":[112],"6700-fold":[114],"D146A).":[116],"accompanied":[121],"by":[122,161],"565-fold":[123],"(D146G)":[124,135],"330-fold":[126],"(D146A)":[127,138],"increases":[128,186],"2-fold":[134],"20-fold":[137],"decreases":[139],"NAD+.":[143],"further":[145],"improve":[146],"Gln147,":[149],"Leu148,":[150],"Leu149,":[151],"Thr192":[153],"enzyme":[155],"D146G":[156],"D146A":[158],"replaced":[160],"lysine":[162],"arginine,":[164],"which":[165],"could":[166],"stabilize":[167],"2'-phosphate":[169],"Enzymes":[172],"D146G/T192K,":[173],"D146G/T192R,":[174],"D146G/L148K,":[175],"D146A/L148K,":[176],"D146A/L148R":[178],"exhibited":[179],"3200-,":[180],"4500-,":[181],"56000-,":[182],"72000-,":[183],"83000-fold":[185],"relative":[192],"wild-type":[195],"enzyme.":[196]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}
