{"id":"https://openalex.org/W2076346277","doi":"https://doi.org/10.3791/52336","title":"Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area","display_name":"Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area","publication_year":2015,"publication_date":"2015-02-06","ids":{"openalex":"https://openalex.org/W2076346277","doi":"https://doi.org/10.3791/52336","mag":"2076346277","pmid":"https://pubmed.ncbi.nlm.nih.gov/25742438","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4354571"},"language":"en","primary_location":{"id":"doi:10.3791/52336","doi":"https://doi.org/10.3791/52336","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52336","pdf_url":"https://www.jove.com/pdf/52336/laser-capture-microdissection-demonstration-isolation-individual","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/52336/laser-capture-microdissection-demonstration-isolation-individual","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078560723","display_name":"Evangel Kummari","orcid":"https://orcid.org/0000-0001-6273-901X","observed_orcids":["https://orcid.org/0000-0001-6273-901X"]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Evangel Kummari","raw_affiliation_strings":["Department of Basic Sciences, Mississippi State University College of Veterinary Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, Mississippi State University College of Veterinary Medicine","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050564187","display_name":"Shirley X. Guo\u2010Ross","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shirley X. Guo-Ross","raw_affiliation_strings":["Department of Basic Sciences, Mississippi State University College of Veterinary Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, Mississippi State University College of Veterinary Medicine","institution_ids":["https://openalex.org/I99041443"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021350442","display_name":"Jeffrey B. Eells","orcid":"https://orcid.org/0000-0002-6381-1666","observed_orcids":["https://orcid.org/0000-0002-6381-1666"]},"institutions":[{"id":"https://openalex.org/I99041443","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872","country_code":"US","type":"education","lineage":["https://openalex.org/I4210141039","https://openalex.org/I99041443"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey B. Eells","raw_affiliation_strings":["Department of Basic Sciences, Mississippi State University College of Veterinary Medicine; eells@cvm.msstate.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Basic Sciences, Mississippi State University College of Veterinary Medicine; eells@cvm.msstate.edu","institution_ids":["https://openalex.org/I99041443"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I99041443"],"apc_list":null,"apc_paid":null,"fwci":1.0121,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.74779933,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":null,"issue":"96","first_page":"e52336","last_page":"e52336"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10056","display_name":"Neurotransmitter Receptor Influence on Behavior","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/laser-capture-microdissection","display_name":"laser capture microdissection","score":1.0},{"id":"https://openalex.org/keywords/dopaminergic-neurons","display_name":"dopaminergic neurons","score":0.9929999709129333},{"id":"https://openalex.org/keywords/ventral-tegmental-area","display_name":"ventral tegmental area","score":1.0},{"id":"https://openalex.org/keywords/tyrosine-hydroxylase","display_name":"tyrosine hydroxylase","score":0.9739999771118164},{"id":"https://openalex.org/keywords/glass-slides","display_name":"glass slides","score":0.6320000290870667},{"id":"https://openalex.org/keywords/tissue-dehydration","display_name":"tissue dehydration","score":0.5619999766349792},{"id":"https://openalex.org/keywords/rna-extraction","display_name":"RNA extraction","score":0.7850000262260437},{"id":"https://openalex.org/keywords/single-cell-isolation","display_name":"single-cell isolation","score":0.6039999723434448},{"id":"https://openalex.org/keywords/brain-tissue","display_name":"brain tissue","score":0.35199999809265137}],"concepts":[{"id":"https://openalex.org/C125965507","wikidata":"https://www.wikidata.org/wiki/Q1516595","display_name":"Laser capture microdissection","level":4,"score":0.8332902789115906},{"id":"https://openalex.org/C2780948874","wikidata":"https://www.wikidata.org/wiki/Q640562","display_name":"Ventral tegmental area","level":4,"score":0.8038743734359741},{"id":"https://openalex.org/C159167319","wikidata":"https://www.wikidata.org/wiki/Q420766","display_name":"Tyrosine hydroxylase","level":3,"score":0.6221432685852051},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5901145339012146},{"id":"https://openalex.org/C11242284","wikidata":"https://www.wikidata.org/wiki/Q4293202","display_name":"Microdissection","level":3,"score":0.527317464351654},{"id":"https://openalex.org/C513476851","wikidata":"https://www.wikidata.org/wiki/Q170304","display_name":"Dopamine","level":2,"score":0.4985380172729492},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4515607953071594},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.44452381134033203},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.42692673206329346},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.336067795753479},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.32209521532058716},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.22796714305877686},{"id":"https://openalex.org/C137183658","wikidata":"https://www.wikidata.org/wiki/Q461076","display_name":"Dopaminergic","level":3,"score":0.20586267113685608},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11873641610145569},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1078522801399231},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.06539243459701538},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017557","descriptor_name":"Ventral Tegmental Area","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059290","descriptor_name":"Dopaminergic Neurons","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D061346","descriptor_name":"Laser Capture Microdissection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.3791/52336","doi":"https://doi.org/10.3791/52336","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52336","pdf_url":"https://www.jove.com/pdf/52336/laser-capture-microdissection-demonstration-isolation-individual","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3322099","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4354571","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4354571","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4354571","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:25742438","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25742438","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3791/52336","doi":"https://doi.org/10.3791/52336","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52336","pdf_url":"https://www.jove.com/pdf/52336/laser-capture-microdissection-demonstration-isolation-individual","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4138030907","display_name":"Regulation and function of Nurr1 in adult nigrostriatal dopamine neurons","funder_award_id":"R21NS058375","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G443812114","display_name":"MRI:  Acquisition of a Laser Microdissection Instrument","funder_award_id":"0723080","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G7397269315","display_name":"Molecular biology of mesocortical and mesoaccumbens dopamine neurons","funder_award_id":"R15MH084209","funder_id":"https://openalex.org/F4320337346","funder_display_name":"National Institute of Mental Health"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309637","display_name":"Mississippi State University","ror":"https://ror.org/0432jq872"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2076346277.pdf","grobid_xml":"https://content.openalex.org/works/W2076346277.grobid-xml"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W1978080759","https://openalex.org/W1980337350","https://openalex.org/W2008655188","https://openalex.org/W2023536589","https://openalex.org/W2029752196","https://openalex.org/W2048181772","https://openalex.org/W2065481692","https://openalex.org/W2096446865","https://openalex.org/W2109847775","https://openalex.org/W2112944608","https://openalex.org/W2154229172","https://openalex.org/W2159174770","https://openalex.org/W2166329962","https://openalex.org/W2186522332","https://openalex.org/W2417802793"],"related_works":["https://openalex.org/W7099970683","https://openalex.org/W4412795130","https://openalex.org/W4286855279","https://openalex.org/W2420891746","https://openalex.org/W2612993491","https://openalex.org/W977557311","https://openalex.org/W1845776741","https://openalex.org/W2140561766","https://openalex.org/W2372825095","https://openalex.org/W3166054107"],"abstract_inverted_index":{"Laser":[0],"capture":[1,97,140],"microdissection":[2],"(LCM)":[3],"is":[4,50,99,133,148,186,193,220,223],"used":[5,34,91,100,149],"to":[6,35,89,101,150],"isolate":[7,36,151],"a":[8,24,42,54,86,173,217],"concentrated":[9],"population":[10,56],"of":[11,18,57,69,75,125,137,191,203,211,214,241,276],"individual":[12,37,104,152],"cells":[13,38,269],"or":[14,155],"precise":[15],"anatomical":[16,290],"regions":[17],"tissue":[19,21,127,205,215,233,267],"from":[20,206,216,266,287],"sections":[22],"on":[23,41,85,196],"microscope":[25],"slide.":[26,208],"When":[27],"combined":[28],"with":[29,62,128,271],"immunohistochemistry,":[30],"LCM":[31,48,119],"can":[32,246,284],"be":[33,247],"types":[39],"based":[40],"specific":[43,55],"protein":[44,278],"marker.":[45],"Here,":[46],"the":[47,70,76,109,126,138,143,156,204,207,261,288],"technique":[49],"described":[51],"for":[52,67,92,121,252],"collecting":[53,184,188],"dopamine":[58,71,153],"neurons":[59,105,154],"directly":[60],"labeled":[61],"tyrosine":[63,82],"hydroxylase":[64,83],"immunohistochemistry":[65,84],"and":[66,116,131,142,183,198,228,244,258,268,274,279,291],"isolation":[68,273],"neuron":[72],"containing":[73],"region":[74],"ventral":[77,110,157],"tegmental":[78,111,158],"area":[79,112,159],"using":[80,161,249,295],"indirect":[81],"section":[87],"adjacent":[88],"those":[90],"LCM.":[93,296],"An":[94],"infrared":[95],"(IR)":[96],"laser":[98,141,147],"both":[102],"dissect":[103],"as":[106,108,176],"well":[107],"off":[113],"glass":[114,174,218],"slides":[115],"onto":[117],"an":[118],"cap":[120],"analysis.":[122],"Complete":[123],"dehydration":[124,197],"100%":[129],"ethanol":[130],"xylene":[132],"critical.":[134],"The":[135],"combination":[136],"IR":[139],"ultraviolet":[144],"(UV)":[145],"cutting":[146],"when":[160],"PEN":[162,166],"membrane":[163,167],"slides.":[164],"A":[165],"slide":[168,175,219],"has":[169],"significant":[170],"advantages":[171],"over":[172],"it":[177,222],"offers":[178],"better":[179],"consistency":[180],"in":[181,200,282],"capturing":[182],"cells,":[185],"faster":[187],"large":[189,212],"pieces":[190],"tissue,":[192],"less":[194],"reliant":[195],"results":[199],"complete":[201],"removal":[202,210],"Although":[209,256],"areas":[213],"feasible,":[221],"considerably":[224],"more":[225],"time":[226],"consuming":[227],"frequently":[229],"leaves":[230],"some":[231],"residual":[232],"behind.":[234],"Data":[235],"shown":[236],"here":[237],"demonstrate":[238],"that":[239],"RNA":[240,257],"sufficient":[242],"quantity":[243],"quality":[245],"obtained":[248,294],"these":[250],"procedures":[251],"quantitative":[253],"PCR":[254],"measurements.":[255],"DNA":[259,283],"are":[260],"most":[262],"commonly":[263],"isolated":[264],"molecules":[265],"collected":[270],"LCM,":[272],"measurement":[275],"microRNA,":[277],"epigenetic":[280],"changes":[281],"also":[285],"benefit":[286],"enhanced":[289],"cellular":[292],"resolution":[293]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}