{"id":"https://openalex.org/W2076284440","doi":"https://doi.org/10.1038/nprot.2008.54","title":"Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites","display_name":"Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites","publication_year":2008,"publication_date":"2008-05-01","ids":{"openalex":"https://openalex.org/W2076284440","doi":"https://doi.org/10.1038/nprot.2008.54","mag":"2076284440","pmid":"https://pubmed.ncbi.nlm.nih.gov/18451799"},"language":"en","primary_location":{"id":"doi:10.1038/nprot.2008.54","doi":"https://doi.org/10.1038/nprot.2008.54","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nprot.2008.54","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007004488","display_name":"Elijahu Berkovich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Elijahu Berkovich","raw_affiliation_strings":["Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061090065","display_name":"Raymond J. Monnat","orcid":"https://orcid.org/0000-0001-7638-7393","observed_orcids":["https://orcid.org/0000-0001-7638-7393"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Raymond J Monnat","raw_affiliation_strings":["Departments of Pathology and Genome Sciences, University of Washington, Box 357705, Seattle, 98195-7705, Washington, USA","Departments of Pathology and Genome Sciences, University of Washington, Box 357705, Seattle, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Pathology and Genome Sciences, University of Washington, Box 357705, Seattle, 98195-7705, Washington, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Departments of Pathology and Genome Sciences, University of Washington, Box 357705, Seattle, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021559507","display_name":"Michael B. Kastan","orcid":"https://orcid.org/0000-0002-1719-7562","observed_orcids":["https://orcid.org/0000-0002-1719-7562"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael B Kastan","raw_affiliation_strings":["Department of Oncology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, 38105, Tennessee, USA","Department of Oncology, St Jude Children\u2019s Research Hospital, Memphis, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oncology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, 38105, Tennessee, USA","institution_ids":["https://openalex.org/I1313298211"]},{"raw_affiliation_string":"Department of Oncology, St Jude Children\u2019s Research Hospital, Memphis, USA","institution_ids":["https://openalex.org/I1313298211"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5021559507"],"corresponding_institution_ids":["https://openalex.org/I1313298211"],"apc_list":null,"apc_paid":null,"fwci":1.2392,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.7678335,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"3","issue":"5","first_page":"915","last_page":"922"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.996999979019165},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.8349999785423279},{"id":"https://openalex.org/keywords/protein-dynamics","display_name":"protein dynamics","score":0.5400000214576721},{"id":"https://openalex.org/keywords/chromatin-immunoprecipitation","display_name":"chromatin immunoprecipitation","score":0.8349999785423279},{"id":"https://openalex.org/keywords/site-specific-dna-cleavage","display_name":"site-specific DNA cleavage","score":0.671999990940094},{"id":"https://openalex.org/keywords/cross-linking","display_name":"crosslinking","score":0.6930000185966492},{"id":"https://openalex.org/keywords/dna-repair-deficiency","display_name":"DNA repair deficiency","score":0.6439999938011169},{"id":"https://openalex.org/keywords/human-cells","display_name":"human cells","score":0.7429999709129333}],"concepts":[{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.8196823000907898},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6394056081771851},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5999997854232788},{"id":"https://openalex.org/C134320426","wikidata":"https://www.wikidata.org/wiki/Q901026","display_name":"Chromatin immunoprecipitation","level":5,"score":0.5834884643554688},{"id":"https://openalex.org/C176619506","wikidata":"https://www.wikidata.org/wiki/Q5065899","display_name":"ChIP-sequencing","level":5,"score":0.5062353014945984},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.49127548933029175},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.48613211512565613},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.47086966037750244},{"id":"https://openalex.org/C197077220","wikidata":"https://www.wikidata.org/wiki/Q720988","display_name":"Human genome","level":4,"score":0.42132651805877686},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3472210168838501},{"id":"https://openalex.org/C167227067","wikidata":"https://www.wikidata.org/wiki/Q5113739","display_name":"Chromatin remodeling","level":4,"score":0.33665770292282104},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.333191454410553},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2665082812309265},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.25815117359161377},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.17227911949157715},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.06423383951187134}],"mesh":[{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004706","descriptor_name":"Endodeoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015336","descriptor_name":"Molecular Probe Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D047369","descriptor_name":"Chromatin Immunoprecipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053903","descriptor_name":"DNA Breaks, Double-Stranded","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1038/nprot.2008.54","doi":"https://doi.org/10.1038/nprot.2008.54","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nprot.2008.54","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},{"id":"pmid:18451799","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18451799","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G626234566","display_name":null,"funder_award_id":"CA48022","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6619561181","display_name":"Functional Characterization of the ATM Gene Product","funder_award_id":"R01CA071387","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G691838600","display_name":"Molecular Determinants of Cancer Therapeutic Response","funder_award_id":"P01CA077852","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000904664","display_name":"Cancer Center Support Grant (CCSG)","funder_award_id":"P30CA021765","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320313598","display_name":"American Lebanese Syrian Associated Charities","ror":"https://ror.org/03rx10x31"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W1488992604","https://openalex.org/W1509538618","https://openalex.org/W1762601366","https://openalex.org/W1773763683","https://openalex.org/W1845461494","https://openalex.org/W1965239527","https://openalex.org/W1967999707","https://openalex.org/W1977088495","https://openalex.org/W1983218764","https://openalex.org/W2012898143","https://openalex.org/W2018848440","https://openalex.org/W2019405732","https://openalex.org/W2027574826","https://openalex.org/W2034831440","https://openalex.org/W2036546456","https://openalex.org/W2106173982","https://openalex.org/W2112327838","https://openalex.org/W2124677401","https://openalex.org/W2142522423","https://openalex.org/W2144252460"],"related_works":["https://openalex.org/W2611499577","https://openalex.org/W4311328706","https://openalex.org/W2047599332","https://openalex.org/W2806857771","https://openalex.org/W2031375145","https://openalex.org/W4415902802","https://openalex.org/W2618216954","https://openalex.org/W3209521703","https://openalex.org/W2281511079","https://openalex.org/W7100528685"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}