{"id":"https://openalex.org/W2075722973","doi":"https://doi.org/10.1021/jp048162v","title":"Heterogeneous Motion of Secretory Vesicles in the Actin Cortex of Live Cells: 3D Tracking to 5-nm Accuracy","display_name":"Heterogeneous Motion of Secretory Vesicles in the Actin Cortex of Live Cells: 3D Tracking to 5-nm Accuracy","publication_year":2004,"publication_date":"2004-10-07","ids":{"openalex":"https://openalex.org/W2075722973","doi":"https://doi.org/10.1021/jp048162v","mag":"2075722973"},"language":"en","primary_location":{"id":"doi:10.1021/jp048162v","doi":"https://doi.org/10.1021/jp048162v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jp048162v","pdf_url":null,"source":{"id":"https://openalex.org/S146709414","display_name":"The Journal of Physical Chemistry A","issn_l":"1089-5639","issn":["1089-5639","1520-5215"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physical Chemistry A","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032538470","display_name":"Michael Konopka","orcid":"https://orcid.org/0000-0002-2194-6300","observed_orcids":["https://orcid.org/0000-0002-2194-6300"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael C. Konopka","raw_affiliation_strings":["Department of Chemistry, University of WisconsinMadison, Madison, Wisconsin 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of WisconsinMadison, Madison, Wisconsin 53706","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077564904","display_name":"James C. Weisshaar","orcid":"https://orcid.org/0000-0002-0922-5113","observed_orcids":["https://orcid.org/0000-0002-0922-5113"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James C. Weisshaar","raw_affiliation_strings":["Department of Chemistry, University of WisconsinMadison, Madison, Wisconsin 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of WisconsinMadison, Madison, Wisconsin 53706","institution_ids":["https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5077564904"],"corresponding_institution_ids":["https://openalex.org/I135310074"],"apc_list":null,"apc_paid":null,"fwci":0.6355,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.64901385,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"108","issue":"45","first_page":"9814","last_page":"9826"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.8823000192642212,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.8823000192642212,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.03009999915957451,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.029500000178813934,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/secretory-vesicles","display_name":"secretory vesicles","score":0.9240000247955322},{"id":"https://openalex.org/keywords/actin-cortex","display_name":"actin cortex","score":0.8759999871253967},{"id":"https://openalex.org/keywords/dense-core-vesicles","display_name":"dense core vesicles","score":0.6549999713897705},{"id":"https://openalex.org/keywords/vesicle-motility","display_name":"vesicle motility","score":0.6499999761581421},{"id":"https://openalex.org/keywords/3d-particle-tracking","display_name":"3D particle tracking","score":0.6769999861717224},{"id":"https://openalex.org/keywords/pc12-cells","display_name":"PC12 cells","score":0.7599999904632568},{"id":"https://openalex.org/keywords/kinesin","display_name":"kinesin","score":0.7039999961853027},{"id":"https://openalex.org/keywords/myosin-v","display_name":"myosin V","score":0.949999988079071},{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.7250000238418579},{"id":"https://openalex.org/keywords/random-walks","display_name":"random walks","score":0.5669999718666077},{"id":"https://openalex.org/keywords/motor-regulation","display_name":"motor regulation","score":0.41600000858306885},{"id":"https://openalex.org/keywords/calcium-signaling","display_name":"calcium signaling","score":0.5479999780654907},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.48500001430511475}],"concepts":[{"id":"https://openalex.org/C2775936607","wikidata":"https://www.wikidata.org/wiki/Q466845","display_name":"Tracking (education)","level":2,"score":0.7409735918045044},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.7263598442077637},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.5478475093841553},{"id":"https://openalex.org/C104114177","wikidata":"https://www.wikidata.org/wiki/Q79782","display_name":"Motion (physics)","level":2,"score":0.5246111154556274},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4939923882484436},{"id":"https://openalex.org/C2777348757","wikidata":"https://www.wikidata.org/wiki/Q2346577","display_name":"Cortex (anatomy)","level":2,"score":0.4829806387424469},{"id":"https://openalex.org/C2909863526","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretory Vesicle","level":4,"score":0.4606265127658844},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.42730313539505005},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.3755859136581421},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36466068029403687},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.35091954469680786},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.31195616722106934},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.27197185158729553},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.22891244292259216},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.15766185522079468},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.14266067743301392},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08931282162666321},{"id":"https://openalex.org/C19417346","wikidata":"https://www.wikidata.org/wiki/Q7922","display_name":"Pedagogy","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/jp048162v","doi":"https://doi.org/10.1021/jp048162v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jp048162v","pdf_url":null,"source":{"id":"https://openalex.org/S146709414","display_name":"The Journal of Physical Chemistry A","issn_l":"1089-5639","issn":["1089-5639","1520-5215"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physical Chemistry A","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W1519523794","https://openalex.org/W1539090868","https://openalex.org/W1558787692","https://openalex.org/W1590230114","https://openalex.org/W1914667261","https://openalex.org/W1964791077","https://openalex.org/W1975852922","https://openalex.org/W1977162001","https://openalex.org/W2000049837","https://openalex.org/W2000335135","https://openalex.org/W2013268726","https://openalex.org/W2014313849","https://openalex.org/W2015404233","https://openalex.org/W2021304137","https://openalex.org/W2024784533","https://openalex.org/W2025042015","https://openalex.org/W2032074620","https://openalex.org/W2040027787","https://openalex.org/W2044419342","https://openalex.org/W2050213416","https://openalex.org/W2055862760","https://openalex.org/W2055870007","https://openalex.org/W2065113965","https://openalex.org/W2067790068","https://openalex.org/W2068724733","https://openalex.org/W2074372974","https://openalex.org/W2080150581","https://openalex.org/W2089434418","https://openalex.org/W2089641450","https://openalex.org/W2096526911","https://openalex.org/W2098456894","https://openalex.org/W2101517266","https://openalex.org/W2105452638","https://openalex.org/W2107299309","https://openalex.org/W2107896719","https://openalex.org/W2112360585","https://openalex.org/W2114336941","https://openalex.org/W2115149094","https://openalex.org/W2119124269","https://openalex.org/W2132218454","https://openalex.org/W2151026886","https://openalex.org/W2167980709","https://openalex.org/W2171362729","https://openalex.org/W2293900557","https://openalex.org/W2321998191","https://openalex.org/W2792028224","https://openalex.org/W2993880730","https://openalex.org/W4236463097"],"related_works":["https://openalex.org/W2120615966","https://openalex.org/W7099889534","https://openalex.org/W1984459297","https://openalex.org/W2585262360","https://openalex.org/W2048293201","https://openalex.org/W1975852922","https://openalex.org/W1996218007","https://openalex.org/W2509444650","https://openalex.org/W2021304137","https://openalex.org/W2093107737"],"abstract_inverted_index":{"Fluorescence":[0],"microscopy":[1],"tracks":[2],"the":[3,17,81,88,91,116,133,175,197,205,232,239,253,262,268,271,293],"three-dimensional":[4],"motion":[5,195],"of":[6,20,37,79,141,165,192,207,226,245,249,255],"green":[7],"fluorescent":[8],"protein":[9],"(GFP)-labeled":[10],"large":[11],"dense-core":[12],"secretory":[13],"vesicles":[14,63,114,177,221,273,298],"(LDCVs)":[15],"within":[16,67,144],"actin":[18],"cortex":[19],"live":[21],"PC12":[22],"cells.":[23],"In":[24],"this":[25],"study,":[26],"we":[27,74],"achieve":[28],"a":[29,34,145,151,155,216,224,282],"26-ms":[30],"time":[31],"resolution":[32],"and":[33,84,106,110,228],"spatial":[35],"accuracy":[36],"5":[38],"nm":[39,251],"or":[40,257,277],"better":[41],"in":[42,48,71,108,154,183,196,204],"each":[43],"dimension":[44],"(one":[45],"standard":[46],"deviation":[47],"one":[49,256],"dimension,":[50],"\u03c3":[51],"1D":[52],").":[53],"The":[54,95,112,243],"resulting":[55],"high-resolution":[56],"trajectories":[57,97],"reveal":[58],"not":[59],"only":[60],"heterogeneity":[61,66,201],"among":[62,90,120],"but":[64,87],"also":[65],"single-vesicle":[68],"trajectories.":[69],"As":[70,168],"earlier":[72],"work,":[73],"observe":[75],"three":[76],"apparent":[77],"groups":[78,92],"vesicles:":[80],"immobile,":[82],"mobile,":[83],"directed-motion":[85],"groups,":[86],"distinctions":[89],"are":[93,274],"blurred.":[94],"directed":[96,194],"exhibit":[98],"segments":[99],"with":[100,124,231],"kinesin-like":[101],"speed":[102,109],"punctuated":[103],"by":[104,138,150,170,223,301],"pauses":[105],"changes":[107],"direction.":[111],"immobile":[113,272],"nearest":[115],"plasma":[117],"membrane":[118],"jump":[119],"sub-25-nm-diameter":[121],"\u201cmini-traps\u201d.":[122],"Comparison":[123],"microrheological":[125],"data":[126],"from":[127],"entangled":[128],"F-actin":[129,241],"solutions":[130],"suggests":[131,252,281],"that":[132,267,288],"jumps":[134],"may":[135,289],"be":[136,290],"caused":[137],"local":[139],"remodeling":[140],"F-actin.":[142],"Motion":[143],"mini-trap":[146],"is":[147,202],"quantitatively":[148],"modeled":[149],"random":[152,181],"walk":[153],"parabolic":[156],"restoring":[157,162],"potential":[158],"to":[159,292,296],"yield":[160],"single-trap":[161],"force":[163],"constants":[164],"\u223c0.04":[166],"pN/nm.":[167],"judged":[169],"mean-square":[171],"displacement":[172],"versus":[173],"time,":[174],"mobile":[176,198,220,263],"execute":[178],"nearly":[179],"free":[180],"walks":[182],"an":[184],"elastic":[185],"medium.":[186],"We":[187,265],"find":[188],"no":[189],"clear":[190],"evidence":[191],"quasi-linear,":[193],"group.":[199],"However,":[200],"evident":[203],"distribution":[206],"frame-to-frame":[208,247],"displacements,":[209],"P":[210],"(":[211],"r":[212],"),":[213],"which":[214],"requires":[215],"two-component":[217],"fit.":[218],"Evidently,":[219],"move":[222],"combination":[225],"diffusion":[227],"motor-driven":[229],"motion,":[230],"direction":[233],"changing":[234],"rapidly":[235],"as":[236],"myosin-V":[237,259],"crisscrosses":[238],"dense":[240],"meshwork.":[242],"frequency":[244],"long":[246],"displacements":[248],"25\u221270":[250],"presence":[254],"more":[258],"motors":[260,269],"on":[261,270],"vesicles.":[264],"argue":[266],"less":[275],"active":[276],"completely":[278],"inactive.":[279],"This":[280],"regulatory":[283],"mechanism":[284],"for":[285],"motor":[286],"activity":[287],"related":[291],"cell's":[294],"ability":[295],"mobilize":[297],"upon":[299],"stimulation":[300],"Ca":[302],"2+":[303],".":[304]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}