{"id":"https://openalex.org/W2074995397","doi":"https://doi.org/10.1107/s1744309109047599","title":"Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted byPseudomonas aeruginosa","display_name":"Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted byPseudomonas aeruginosa","publication_year":2009,"publication_date":"2009-12-24","ids":{"openalex":"https://openalex.org/W2074995397","doi":"https://doi.org/10.1107/s1744309109047599","mag":"2074995397","pmid":"https://pubmed.ncbi.nlm.nih.gov/20057063","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2805529"},"language":"en","primary_location":{"id":"doi:10.1107/s1744309109047599","doi":"https://doi.org/10.1107/s1744309109047599","is_oa":true,"landing_page_url":"https://doi.org/10.1107/s1744309109047599","pdf_url":"http://journals.iucr.org/f/issues/2010/01/00/ll5210/ll5210.pdf","source":{"id":"https://openalex.org/S4210231712","display_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","issn_l":"1744-3091","issn":["1744-3091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://journals.iucr.org/f/issues/2010/01/00/ll5210/ll5210.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050379643","display_name":"Christopher D. Bahl","orcid":"https://orcid.org/0000-0002-3652-3693","observed_orcids":["https://orcid.org/0000-0002-3652-3693"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher D. Bahl","raw_affiliation_strings":["Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055196460","display_name":"Daniel P. MacEachran","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel P. MacEachran","raw_affiliation_strings":["Department of Microbiology and Immunology, Dartmouth Medical School, 7550 Vail Building, Hanover, NH 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Dartmouth Medical School, 7550 Vail Building, Hanover, NH 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036077732","display_name":"George A. O\u2019Toole","orcid":"https://orcid.org/0000-0002-2861-4392","observed_orcids":["https://orcid.org/0000-0002-2861-4392"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George A. O'Toole","raw_affiliation_strings":["Department of Microbiology and Immunology, Dartmouth Medical School, 7550 Vail Building, Hanover, NH 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Dartmouth Medical School, 7550 Vail Building, Hanover, NH 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5035077570","display_name":"Dean R. Madden","orcid":"https://orcid.org/0000-0002-1810-6984","observed_orcids":["https://orcid.org/0000-0002-1810-6984"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dean R. Madden","raw_affiliation_strings":["Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I107672454"],"apc_list":null,"apc_paid":null,"fwci":0.4827,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.63749474,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"66","issue":"1","first_page":"26","last_page":"28"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10665","display_name":"Cystic Fibrosis Research Advances","score":0.016599999740719795,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cif","display_name":"CIF","score":0.9129999876022339},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9440000057220459},{"id":"https://openalex.org/keywords/virulence-factors","display_name":"virulence factors","score":0.8790000081062317},{"id":"https://openalex.org/keywords/cftr-degradation","display_name":"CFTR degradation","score":0.8379999995231628},{"id":"https://openalex.org/keywords/epoxide-hydrolase","display_name":"epoxide hydrolase","score":0.8970000147819519},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6110000014305115},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.8500000238418579},{"id":"https://openalex.org/keywords/vapor-diffusion","display_name":"vapor diffusion","score":0.625},{"id":"https://openalex.org/keywords/airway-colonization","display_name":"airway colonization","score":0.6340000033378601},{"id":"https://openalex.org/keywords/cystic-fibrosis","display_name":"cystic fibrosis","score":0.8629999756813049},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.32100000977516174}],"concepts":[{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.7809522151947021},{"id":"https://openalex.org/C2781090273","wikidata":"https://www.wikidata.org/wiki/Q1623405","display_name":"Virulence factor","level":4,"score":0.6486846208572388},{"id":"https://openalex.org/C2778428886","wikidata":"https://www.wikidata.org/wiki/Q420470","display_name":"Cystic fibrosis transmembrane conductance regulator","level":3,"score":0.5636671781539917},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.5578067302703857},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.5062998533248901},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.46582505106925964},{"id":"https://openalex.org/C2776938444","wikidata":"https://www.wikidata.org/wiki/Q178194","display_name":"Cystic fibrosis","level":2,"score":0.461868554353714},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43392831087112427},{"id":"https://openalex.org/C44312359","wikidata":"https://www.wikidata.org/wiki/Q286958","display_name":"ATP-binding cassette transporter","level":4,"score":0.4241846203804016},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.42414456605911255},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.41957640647888184},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41499239206314087},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3658143877983093},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27681708335876465},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.2037208378314972},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.19826331734657288},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18214434385299683},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1328703761100769},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D037521","descriptor_name":"Virulence Factors","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D037521","descriptor_name":"Virulence Factors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1107/s1744309109047599","doi":"https://doi.org/10.1107/s1744309109047599","is_oa":true,"landing_page_url":"https://doi.org/10.1107/s1744309109047599","pdf_url":"http://journals.iucr.org/f/issues/2010/01/00/ll5210/ll5210.pdf","source":{"id":"https://openalex.org/S4210231712","display_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","issn_l":"1744-3091","issn":["1744-3091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2805529","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2805529","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallogr Sect F Struct Biol Cryst Commun","raw_type":"Text"},{"id":"pmid:20057063","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20057063","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta crystallographica. Section F, Structural biology and crystallization communications","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1107/s1744309109047599","doi":"https://doi.org/10.1107/s1744309109047599","is_oa":true,"landing_page_url":"https://doi.org/10.1107/s1744309109047599","pdf_url":"http://journals.iucr.org/f/issues/2010/01/00/ll5210/ll5210.pdf","source":{"id":"https://openalex.org/S4210231712","display_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","issn_l":"1744-3091","issn":["1744-3091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4000000059604645,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1894282356","display_name":"Renal Function and Disease","funder_award_id":"5t32dk007301-35","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2676936577","display_name":null,"funder_award_id":"T32-DK007301","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G307801351","display_name":"Keeping CFTR in its Place: An Integrated Small-Molecule Approach","funder_award_id":"R01DK075309","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G3180572666","display_name":null,"funder_award_id":"T32-DK00730","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G9000863065","display_name":"Host-Microbe Interactions","funder_award_id":"T32AI007519","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2074995397.pdf","grobid_xml":"https://content.openalex.org/works/W2074995397.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W1568537742","https://openalex.org/W1943327251","https://openalex.org/W1973890165","https://openalex.org/W1987124713","https://openalex.org/W1992321568","https://openalex.org/W1999075212","https://openalex.org/W2000840258","https://openalex.org/W2001641653","https://openalex.org/W2054572399","https://openalex.org/W2066996399","https://openalex.org/W2097493124","https://openalex.org/W2099381109","https://openalex.org/W2109184569","https://openalex.org/W2117245075","https://openalex.org/W2124737508","https://openalex.org/W2146799440","https://openalex.org/W2171286149","https://openalex.org/W2414307244"],"related_works":["https://openalex.org/W2140285444","https://openalex.org/W2171286149","https://openalex.org/W4249532553","https://openalex.org/W2087667790","https://openalex.org/W2762433322","https://openalex.org/W1119901167","https://openalex.org/W4300818844","https://openalex.org/W2290496503","https://openalex.org/W2291380965","https://openalex.org/W2028134905"],"abstract_inverted_index":{"The":[0,93,118],"opportunistic":[1],"pathogen":[2],"Pseudomonas":[3],"aeruginosa":[4],"secretes":[5],"a":[6,38,103,126],"protein":[7],"that":[8,140],"triggers":[9],"the":[10,14,31,66,71,78,82,131,141],"accelerated":[11],"degradation":[12],"of":[13,65,74,77,84],"cystic":[15],"fibrosis":[16],"transmembrane":[17],"conductance":[18],"regulator":[19],"(CFTR)":[20],"in":[21,70,96],"airway":[22,44],"epithelial":[23],"cells.":[24],"This":[25],"protein,":[26],"which":[27],"is":[28,91],"known":[29],"as":[30,37],"CFTR":[32],"inhibitory":[33],"factor":[34,40],"(Cif),":[35],"acts":[36],"virulence":[39],"and":[41],"may":[42],"facilitate":[43],"colonization":[45],"by":[46,88],"P.":[47],"aeruginosa.":[48],"Based":[49,129],"on":[50,125,130],"sequence":[51],"similarity":[52],"Cif":[53,87],"appears":[54,139],"to":[55,121],"be":[56],"an":[57],"epoxide":[58],"hydrolase":[59],"(EH),":[60],"but":[61],"it":[62,138],"lacks":[63],"several":[64],"conserved":[67],"features":[68],"found":[69],"active":[72],"sites":[73],"canonical":[75],"members":[76],"EH":[79],"family.":[80],"Here,":[81],"crystallization":[83],"purified":[85],"recombinant":[86],"vapor":[89],"diffusion":[90],"reported.":[92],"crystals":[94,119],"formed":[95],"space":[97],"group":[98],"C2,":[99],"with":[100],"unit-cell":[101],"parameters":[102],"=":[104,107,110,114],"167.4,":[105],"b":[106],"83.6,":[108],"c":[109],"88.3":[111],"A,":[112],"beta":[113],"100.6":[115],"degrees":[116],".":[117],"diffracted":[120],"2.39":[122],"A":[123],"resolution":[124],"rotating-anode":[127],"source.":[128],"calculated":[132],"Matthews":[133],"coefficient":[134],"(2.2":[135],"A(3)":[136],"Da(-1)),":[137],"asymmetric":[142],"unit":[143],"contains":[144],"four":[145],"molecules.":[146]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}