{"id":"https://openalex.org/W2074589363","doi":"https://doi.org/10.1126/science.2154850","title":"Functional Properties of Rat Brain Sodium Channels Expressed in a Somatic Cell Line","display_name":"Functional Properties of Rat Brain Sodium Channels Expressed in a Somatic Cell Line","publication_year":1990,"publication_date":"1990-02-16","ids":{"openalex":"https://openalex.org/W2074589363","doi":"https://doi.org/10.1126/science.2154850","mag":"2074589363","pmid":"https://pubmed.ncbi.nlm.nih.gov/2154850"},"language":"en","primary_location":{"id":"doi:10.1126/science.2154850","doi":"https://doi.org/10.1126/science.2154850","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.2154850","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002307585","display_name":"Todd Scheuer","orcid":"https://orcid.org/0000-0003-3655-0816","observed_orcids":["https://orcid.org/0000-0003-3655-0816"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Todd Scheuer","raw_affiliation_strings":["Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063077142","display_name":"Vanessa J. Auld","orcid":"https://orcid.org/0000-0003-3976-9186","observed_orcids":["https://orcid.org/0000-0003-3976-9186"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Vanessa J. Auld","raw_affiliation_strings":["Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111392520","display_name":"Shelley Romayne Boyd","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Shelley Boyd","raw_affiliation_strings":["Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039320169","display_name":"James Offord","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James Offord","raw_affiliation_strings":["Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Robert Dunn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Robert Dunn","raw_affiliation_strings":["Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departnent of Medical Genetics, University ofToronto,Toronto, Ontario, Canada M5S lAl","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078941732","display_name":"William A. Catterall","orcid":"https://orcid.org/0000-0001-5509-3580","observed_orcids":["https://orcid.org/0000-0001-5509-3580"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William A. Catterall","raw_affiliation_strings":["Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, School of Medicine, University of Washington, Seattle, WA 98195","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.708,"has_fulltext":false,"cited_by_count":102,"citation_normalized_percentile":{"value":0.94308102,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"247","issue":"4944","first_page":"854","last_page":"858"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sodium-channels","display_name":"sodium channels","score":0.9440000057220459},{"id":"https://openalex.org/keywords/rat-brain","display_name":"rat brain","score":0.7450000047683716},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.6970000267028809},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.9470000267028809},{"id":"https://openalex.org/keywords/sodium-current","display_name":"sodium current","score":0.6740000247955322},{"id":"https://openalex.org/keywords/tetrodotoxin","display_name":"tetrodotoxin","score":0.8659999966621399},{"id":"https://openalex.org/keywords/veratridine","display_name":"veratridine","score":0.9940000176429749},{"id":"https://openalex.org/keywords/cdna-expression","display_name":"cDNA expression","score":0.49799999594688416}],"concepts":[{"id":"https://openalex.org/C2776986425","wikidata":"https://www.wikidata.org/wiki/Q721202","display_name":"Veratridine","level":4,"score":0.9506913423538208},{"id":"https://openalex.org/C50952357","wikidata":"https://www.wikidata.org/wiki/Q424960","display_name":"Sodium channel","level":3,"score":0.887455403804779},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.8621034622192383},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7456907629966736},{"id":"https://openalex.org/C2780358027","wikidata":"https://www.wikidata.org/wiki/Q407752","display_name":"Neurotoxin","level":2,"score":0.5779520273208618},{"id":"https://openalex.org/C2776741303","wikidata":"https://www.wikidata.org/wiki/Q379842","display_name":"Tetrodotoxin","level":2,"score":0.5618154406547546},{"id":"https://openalex.org/C83730767","wikidata":"https://www.wikidata.org/wiki/Q24778884","display_name":"G alpha subunit","level":4,"score":0.511246919631958},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5085834264755249},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.504158616065979},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4980595111846924},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4623732566833496},{"id":"https://openalex.org/C134305767","wikidata":"https://www.wikidata.org/wiki/Q725508","display_name":"Somatic cell","level":3,"score":0.43538498878479004},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.415774941444397},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.37685489654541016},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37506920099258423},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3640947937965393},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3033868670463562},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.28134679794311523},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1354537010192871},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.082416832447052},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004553","descriptor_name":"Electric Conductivity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D010053","descriptor_name":"Ovary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013779","descriptor_name":"Tetrodotoxin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015222","descriptor_name":"Sodium Channels","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D015222","descriptor_name":"Sodium Channels","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1126/science.2154850","doi":"https://doi.org/10.1126/science.2154850","is_oa":false,"landing_page_url":"https://doi.org/10.1126/science.2154850","pdf_url":null,"source":{"id":"https://openalex.org/S3880285","display_name":"Science","issn_l":"0036-8075","issn":["0036-8075","0193-4511","1095-9203"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315823","host_organization_name":"American Association for the Advancement of Science","host_organization_lineage":["https://openalex.org/P4310315823"],"host_organization_lineage_names":["American Association for the Advancement of Science"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science","raw_type":"journal-article"},{"id":"pmid:2154850","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2154850","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science (New York, N.Y.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4000000059604645,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G6737517775","display_name":"Voltage Sensitive Sodium Channels in Brain","funder_award_id":"R01NS015751","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8332189701","display_name":"Cell Biology of the Neuronal Sodium Channel","funder_award_id":"R01NS025704","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W53028382","https://openalex.org/W1144752480","https://openalex.org/W1553386262","https://openalex.org/W1922482662","https://openalex.org/W2004002713","https://openalex.org/W2006192148","https://openalex.org/W2009667219","https://openalex.org/W2011657784","https://openalex.org/W2030156849","https://openalex.org/W2037341552","https://openalex.org/W2042036078","https://openalex.org/W2065639286","https://openalex.org/W2065928015","https://openalex.org/W2066082364","https://openalex.org/W2075037235","https://openalex.org/W2076892293","https://openalex.org/W2078119356","https://openalex.org/W2094362927","https://openalex.org/W2104473432","https://openalex.org/W2106940870","https://openalex.org/W2118085774","https://openalex.org/W2120771179","https://openalex.org/W2129664902","https://openalex.org/W2132181180","https://openalex.org/W2152016695","https://openalex.org/W2153267101","https://openalex.org/W2163279702","https://openalex.org/W2219473100","https://openalex.org/W2409424606","https://openalex.org/W2888820767","https://openalex.org/W4234550168"],"related_works":["https://openalex.org/W634243829","https://openalex.org/W2037384321","https://openalex.org/W2150895802","https://openalex.org/W1994944361","https://openalex.org/W2077678479","https://openalex.org/W7096067730","https://openalex.org/W2263696197","https://openalex.org/W2115044183","https://openalex.org/W2170453572","https://openalex.org/W2146958340"],"abstract_inverted_index":{"Transfection":[0],"of":[1,22,34,88],"Chinese":[2],"hamster":[3],"ovary":[4],"cells":[5],"with":[6,26,92],"complementary":[7],"DNA":[8],"encoding":[9],"the":[10,27,93],"RIIA":[11,76],"sodium":[12,24,35,41,77,90],"channel":[13,78],"alpha":[14,79],"subunit":[15,80],"from":[16],"rat":[17],"brain":[18,38],"led":[19],"to":[20],"expression":[21,87],"functional":[23,73,89],"channels":[25,36,47,91],"rapid,":[28],"voltage-dependent":[29],"activation":[30],"and":[31,56,96],"inactivation":[32],"characteristic":[33],"in":[37,72,99],"neurons.":[39],"The":[40,75],"currents":[42],"mediated":[43],"by":[44,50,54,58],"these":[45],"transfected":[46],"were":[48,70],"inhibited":[49],"tetrodotoxin,":[51],"persistently":[52],"activated":[53],"veratridine,":[55],"prolonged":[57],"Leiurus":[59],"alpha-scorpion":[60],"toxin,":[61],"indicating":[62],"that":[63],"neurotoxin":[64],"receptor":[65],"sites":[66],"1":[67],"through":[68],"3":[69],"present":[71],"form.":[74],"cDNA":[81],"alone":[82],"is":[83],"sufficient":[84],"for":[85],"stable":[86],"expected":[94],"kinetic":[95],"pharmacological":[97],"properties":[98],"mammalian":[100],"somatic":[101],"cells.":[102]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}