{"id":"https://openalex.org/W2074215917","doi":"https://doi.org/10.1042/ba20040040","title":"Effects of buffer properties on cyclodextrin glucanotransferase reactions and cyclodextrin production from raw sago (Cycas revoluta) starch","display_name":"Effects of buffer properties on cyclodextrin glucanotransferase reactions and cyclodextrin production from raw sago (Cycas revoluta) starch","publication_year":2005,"publication_date":"2005-03-22","ids":{"openalex":"https://openalex.org/W2074215917","doi":"https://doi.org/10.1042/ba20040040","mag":"2074215917","pmid":"https://pubmed.ncbi.nlm.nih.gov/15202937"},"language":"en","primary_location":{"id":"doi:10.1042/ba20040040","doi":"https://doi.org/10.1042/ba20040040","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20040040","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108085498","display_name":"Kamarulzaman Kamaruddin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I23726118","display_name":"Sirim Berhad","ror":"https://ror.org/008g13a47","country_code":"MY","type":"government","lineage":["https://openalex.org/I23726118"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Kamarulzaman Kamaruddin","raw_affiliation_strings":["Environment and Bioprocess Technology Centre, SIRIM Berhad, Pusat Tenaga, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Environment and Bioprocess Technology Centre, SIRIM Berhad, Pusat Tenaga, Malaysia","institution_ids":["https://openalex.org/I23726118"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037704338","display_name":"Rosli Md Illias","orcid":"https://orcid.org/0000-0001-5577-5821","observed_orcids":["https://orcid.org/0000-0001-5577-5821"]},"institutions":[{"id":"https://openalex.org/I4576418","display_name":"University of Technology Malaysia","ror":"https://ror.org/026w31v75","country_code":"MY","type":"education","lineage":["https://openalex.org/I4576418"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Rosli Md. Illias","raw_affiliation_strings":["\u2020Faculty of Chemical Engineering and Natural Resources, Universiti Teknologi Malaysi, 81310 UTM-Skudai, Johor, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u2020Faculty of Chemical Engineering and Natural Resources, Universiti Teknologi Malaysi, 81310 UTM-Skudai, Johor, Malaysia","institution_ids":["https://openalex.org/I4576418"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113515450","display_name":"Suraini Abd. Aziz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130343225","display_name":"Universiti Putra Malaysia","ror":"https://ror.org/02e91jd64","country_code":"MY","type":"education","lineage":["https://openalex.org/I130343225"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Suraini Abdul Aziz","raw_affiliation_strings":["Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia","institution_ids":["https://openalex.org/I130343225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111630425","display_name":"Mamot Said","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I885383172","display_name":"National University of Malaysia","ror":"https://ror.org/00bw8d226","country_code":"MY","type":"education","lineage":["https://openalex.org/I885383172"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Mamot Said","raw_affiliation_strings":["\u00a7School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysi, 43600 UKM-Bangi, Selangor, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u00a7School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysi, 43600 UKM-Bangi, Selangor, Malaysia","institution_ids":["https://openalex.org/I885383172"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047065643","display_name":"Osman Hassan","orcid":"https://orcid.org/0000-0002-4828-4255","observed_orcids":["https://orcid.org/0000-0002-4828-4255"]},"institutions":[{"id":"https://openalex.org/I885383172","display_name":"National University of Malaysia","ror":"https://ror.org/00bw8d226","country_code":"MY","type":"education","lineage":["https://openalex.org/I885383172"]}],"countries":["MY"],"is_corresponding":true,"raw_author_name":"Osman Hassan","raw_affiliation_strings":["\u00a7School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysi, 43600 UKM-Bangi, Selangor, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u00a7School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysi, 43600 UKM-Bangi, Selangor, Malaysia","institution_ids":["https://openalex.org/I885383172"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5047065643"],"corresponding_institution_ids":["https://openalex.org/I885383172"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":1.1584,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.6978901,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"41","issue":"2","first_page":"117","last_page":"125"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.009499999694526196,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyclodextrin-glycosyltransferase","display_name":"cyclodextrin glycosyltransferase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/sago-starch","display_name":"sago starch","score":0.6930000185966492},{"id":"https://openalex.org/keywords/cycas-revoluta","display_name":"Cycas revoluta","score":0.9890000224113464},{"id":"https://openalex.org/keywords/beta-cyclodextrin","display_name":"\u03b2-cyclodextrin","score":0.8899999856948853},{"id":"https://openalex.org/keywords/alpha-cyclodextrin","display_name":"\u03b1-cyclodextrin","score":0.9629999995231628},{"id":"https://openalex.org/keywords/buffering-effect","display_name":"buffering effect","score":0.7149999737739563},{"id":"https://openalex.org/keywords/ph","display_name":"pH","score":0.6100000143051147},{"id":"https://openalex.org/keywords/ionic-strength","display_name":"ionic strength","score":0.9089999794960022},{"id":"https://openalex.org/keywords/phosphate-buffer","display_name":"phosphate buffer","score":0.7279999852180481},{"id":"https://openalex.org/keywords/tris-hcl-buffer","display_name":"Tris-HCl buffer","score":0.9430000185966492},{"id":"https://openalex.org/keywords/sodium-acetate-buffer","display_name":"sodium acetate buffer","score":0.9010000228881836},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.6079999804496765},{"id":"https://openalex.org/keywords/product-specificity","display_name":"product specificity","score":0.7940000295639038},{"id":"https://openalex.org/keywords/chloride","display_name":"chloride","score":0.5789999961853027},{"id":"https://openalex.org/keywords/calcium-binding-sites","display_name":"calcium binding sites","score":0.7490000128746033},{"id":"https://openalex.org/keywords/gelatinized-starch","display_name":"gelatinized starch","score":0.8009999990463257}],"concepts":[{"id":"https://openalex.org/C2779433975","wikidata":"https://www.wikidata.org/wiki/Q40417805","display_name":"Cyclodextrin","level":2,"score":0.8266760110855103},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7314736843109131},{"id":"https://openalex.org/C2781014771","wikidata":"https://www.wikidata.org/wiki/Q413961","display_name":"Tris","level":2,"score":0.7145733833312988},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.6370788812637329},{"id":"https://openalex.org/C187428577","wikidata":"https://www.wikidata.org/wiki/Q898396","display_name":"Ionic strength","level":3,"score":0.556111216545105},{"id":"https://openalex.org/C2777132085","wikidata":"https://www.wikidata.org/wiki/Q46220103","display_name":"Phosphate","level":2,"score":0.5386464595794678},{"id":"https://openalex.org/C2778117296","wikidata":"https://www.wikidata.org/wiki/Q208465","display_name":"Buffer solution","level":2,"score":0.4850148856639862},{"id":"https://openalex.org/C529335014","wikidata":"https://www.wikidata.org/wiki/Q41534","display_name":"Starch","level":2,"score":0.47914594411849976},{"id":"https://openalex.org/C154881586","wikidata":"https://www.wikidata.org/wiki/Q898579","display_name":"Ionic liquid","level":3,"score":0.42712342739105225},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4059463143348694},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.39386147260665894},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.3404708504676819},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.2854406237602234},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20550307631492615},{"id":"https://openalex.org/C184651966","wikidata":"https://www.wikidata.org/wiki/Q906356","display_name":"Aqueous solution","level":2,"score":0.10870817303657532},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.05562809109687805},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002021","descriptor_name":"Buffers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003505","descriptor_name":"Cyclodextrins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012996","descriptor_name":"Solutions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013213","descriptor_name":"Starch","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013213","descriptor_name":"Starch","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029784","descriptor_name":"Cycas","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1042/ba20040040","doi":"https://doi.org/10.1042/ba20040040","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20040040","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:15202937","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15202937","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322436","display_name":"Novo Nordisk","ror":"https://ror.org/0435rc536"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1828377055","https://openalex.org/W1970052865","https://openalex.org/W1977106104","https://openalex.org/W1982868606","https://openalex.org/W1999969376","https://openalex.org/W2013939197","https://openalex.org/W2055226536","https://openalex.org/W2055350929","https://openalex.org/W2071245559","https://openalex.org/W2093348832","https://openalex.org/W2113473027","https://openalex.org/W2115276160","https://openalex.org/W2125022290","https://openalex.org/W2136508320","https://openalex.org/W2142921025","https://openalex.org/W2151260338","https://openalex.org/W2155561009","https://openalex.org/W2164767252","https://openalex.org/W2166532723","https://openalex.org/W2167659076","https://openalex.org/W2276455722","https://openalex.org/W2323928942","https://openalex.org/W2886090971","https://openalex.org/W4255341024"],"related_works":["https://openalex.org/W2143448076","https://openalex.org/W1981136325","https://openalex.org/W2062389654","https://openalex.org/W2904074710","https://openalex.org/W819062274","https://openalex.org/W2066656276","https://openalex.org/W2032146986","https://openalex.org/W2140196181","https://openalex.org/W7036537226","https://openalex.org/W2604327366"],"abstract_inverted_index":{"Results":[0],"from":[1,62,74,83],"the":[2,8,20,50,72,79,91,107,124,138,181,187,191,195,198,210],"present":[3],"study":[4],"have":[5],"shown":[6],"that":[7],"ionic":[9,139,154],"species":[10],"of":[11,27,57,81,96,109,141,156,168,183,190,197],"buffers,":[12],"pH":[13,44,118,148,171,200],"values":[14],"and":[15,24,88,99,120,144,150,212],"reaction":[16,40],"temperature":[17],"can":[18],"affect":[19],"enzyme":[21],"unit":[22],"activities":[23,211],"product":[25,92],"specificity":[26],"Toruzyme":[28],"(Novo":[29],"Nordisk":[30],"A/S":[31],"Bagsvaerd,":[32],"Denmark)":[33],"CGTase":[34,51],"(cyclodextrin":[35],"glucanotransferase).":[36],"Applying":[37],"a":[38,131],"similar":[39],"environment":[41],"(acetate":[42],"buffer,":[43],"6.0;":[45],"temperature,":[46],"60":[47],"degrees":[48],"C),":[49],"was":[52,114,175],"found":[53],"to":[54,76,85,126,152,180,202,206],"be":[55,178],"capable":[56],"producing":[58],"pre":[59],"dominantly":[60],"beta-cyclodextrin":[61,82,135],"either":[63],"raw":[64],"or":[65],"gelatinized":[66],"sago":[67],"(Cycas":[68],"revoluta)":[69],"starch.":[70],"Changing":[71],"buffer":[73,113,125],"acetate":[75,143],"phosphate":[77,112,157],"reduced":[78],"yield":[80,167],"2.48":[84],"1.42":[86],"mg/ml":[87],"also":[89],"affected":[90],"specificity,":[93],"where":[94],"production":[95,108],"both":[97,117],"alpha-":[98],"beta-cyclodextrins":[100],"were":[101],"more":[102],"pronounced.":[103],"The":[104],"decrease":[105],"in":[106,111,134,194,209],"cyclodextrins":[110,169],"significant":[115,132,161],"at":[116,147,170,186],"6.0":[119,149],"7.0.":[121],"However,":[122],"changing":[123],"Tris/HCl":[127,145,174],"(pH":[128],"7.0)":[129],"showed":[130,159],"increase":[133,208],"production.":[136,165],"Increasing":[137],"strength":[140,155],"sodium":[142],"buffers":[146,158],"7.0":[151,172],"equivalent":[153],"no":[160],"effects":[162],"on":[163],"cyclodextrin":[164],"Higher":[166],"when":[173],"used":[176],"might":[177],"due":[179],"binding":[182],"chloride":[184],"ions":[185],"calcium-binding":[188],"sites":[189],"CGTase,":[192],"resulting":[193],"shift":[196],"optimum":[199],"close":[201],"physiological":[203],"environment,":[204],"leading":[205],"an":[207],"specificity.":[213]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}