{"id":"https://openalex.org/W2073668857","doi":"https://doi.org/10.1002/bit.21947","title":"A high\u2010throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors","display_name":"A high\u2010throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors","publication_year":2008,"publication_date":"2008-04-24","ids":{"openalex":"https://openalex.org/W2073668857","doi":"https://doi.org/10.1002/bit.21947","mag":"2073668857","pmid":"https://pubmed.ncbi.nlm.nih.gov/18500764"},"language":"en","primary_location":{"id":"doi:10.1002/bit.21947","doi":"https://doi.org/10.1002/bit.21947","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.21947","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101728150","display_name":"Huimin Yu","orcid":"https://orcid.org/0000-0002-8164-9826","observed_orcids":["https://orcid.org/0000-0002-8164-9826"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Huimin Yu","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","Department of Chemical Engineering, Tsinghua University, Beijing, China","The author was a visiting scholar when she completed this research","fax: 617-253-3122","telephone: 617-253-4583"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"The author was a visiting scholar when she completed this research","institution_ids":[]},{"raw_affiliation_string":"fax: 617-253-3122","institution_ids":[]},{"raw_affiliation_string":"telephone: 617-253-4583","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049701357","display_name":"Keith E. J. Tyo","orcid":"https://orcid.org/0000-0002-2342-0687","observed_orcids":["https://orcid.org/0000-0002-2342-0687"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Keith Tyo","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","fax: 617-253-3122","telephone: 617-253-4583"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"fax: 617-253-3122","institution_ids":[]},{"raw_affiliation_string":"telephone: 617-253-4583","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023917549","display_name":"Hal S. Alper","orcid":"https://orcid.org/0000-0002-8246-8605","observed_orcids":["https://orcid.org/0000-0002-8246-8605"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hal Alper","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","fax: 617-253-3122","telephone: 617-253-4583"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"fax: 617-253-3122","institution_ids":[]},{"raw_affiliation_string":"telephone: 617-253-4583","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035667850","display_name":"Daniel Klein\u2010Marcuschamer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel Klein\u2010Marcuschamer","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","fax: 617-253-3122","telephone: 617-253-4583"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"fax: 617-253-3122","institution_ids":[]},{"raw_affiliation_string":"telephone: 617-253-4583","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009763658","display_name":"Gregory N Stephanopoulos","orcid":"https://orcid.org/0000-0001-6909-4568","observed_orcids":["https://orcid.org/0000-0001-6909-4568"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory Stephanopoulos","raw_affiliation_strings":["Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","fax: 617-253-3122","telephone: 617-253-4583"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; telephone: 617-253-4583; fax: 617-253-3122","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"fax: 617-253-3122","institution_ids":[]},{"raw_affiliation_string":"telephone: 617-253-4583","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5009763658"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":2.7487,"has_fulltext":false,"cited_by_count":59,"citation_normalized_percentile":{"value":0.89132799,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"101","issue":"4","first_page":"788","last_page":"796"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.44190001487731934,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.44190001487731934,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.24060000479221344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.06669999659061432,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hyaluronic-acid-production","display_name":"hyaluronic acid production","score":0.9210000038146973},{"id":"https://openalex.org/keywords/recombinant-escherichia-coli","display_name":"recombinant Escherichia coli","score":0.9959999918937683},{"id":"https://openalex.org/keywords/sigma-factor","display_name":"sigma factor","score":0.6679999828338623},{"id":"https://openalex.org/keywords/rpod","display_name":"rpoD","score":0.9100000262260437},{"id":"https://openalex.org/keywords/rpos","display_name":"RpoS","score":0.9980000257492065},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7590000033378601},{"id":"https://openalex.org/keywords/alcian-blue-staining","display_name":"Alcian blue staining","score":0.9010000228881836},{"id":"https://openalex.org/keywords/strain-selection","display_name":"strain selection","score":0.5519999861717224},{"id":"https://openalex.org/keywords/biopolymer-production","display_name":"biopolymer production","score":0.4480000138282776}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7927354574203491},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6663176417350769},{"id":"https://openalex.org/C2777712861","wikidata":"https://www.wikidata.org/wiki/Q7375543","display_name":"rpoS","level":5,"score":0.6375071406364441},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.5830134749412537},{"id":"https://openalex.org/C98015330","wikidata":"https://www.wikidata.org/wiki/Q907315","display_name":"Absorbance","level":2,"score":0.5814588069915771},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5587349534034729},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4526292681694031},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3241426348686218},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3040144443511963},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26981741189956665},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.18281173706054688},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12276950478553772},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.08186715841293335},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006820","descriptor_name":"Hyaluronic Acid","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012808","descriptor_name":"Sigma Factor","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050505","descriptor_name":"Mutant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D050505","descriptor_name":"Mutant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.21947","doi":"https://doi.org/10.1002/bit.21947","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.21947","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18500764","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18500764","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1509786858","https://openalex.org/W1553047309","https://openalex.org/W1560298788","https://openalex.org/W1583378819","https://openalex.org/W1589714231","https://openalex.org/W1781531090","https://openalex.org/W1894848931","https://openalex.org/W1985920238","https://openalex.org/W1986198902","https://openalex.org/W1986947369","https://openalex.org/W2014357380","https://openalex.org/W2023767700","https://openalex.org/W2023911414","https://openalex.org/W2034008313","https://openalex.org/W2036447433","https://openalex.org/W2039251116","https://openalex.org/W2041183456","https://openalex.org/W2055088522","https://openalex.org/W2062064581","https://openalex.org/W2070528883","https://openalex.org/W2075051217","https://openalex.org/W2080537888","https://openalex.org/W2100491597","https://openalex.org/W2109918485","https://openalex.org/W2111742728","https://openalex.org/W2115024759","https://openalex.org/W2115785530","https://openalex.org/W2131921511","https://openalex.org/W2136364313","https://openalex.org/W2137245962","https://openalex.org/W2145617716","https://openalex.org/W2150804231","https://openalex.org/W2168989431","https://openalex.org/W2414997889","https://openalex.org/W3169629205"],"related_works":["https://openalex.org/W2612644771","https://openalex.org/W2389782166","https://openalex.org/W2051721236","https://openalex.org/W4310205498","https://openalex.org/W2978289313","https://openalex.org/W2375535364","https://openalex.org/W2389255651","https://openalex.org/W2370345891","https://openalex.org/W2022792456","https://openalex.org/W2067171708"],"abstract_inverted_index":{"Hyaluronic":[0],"acid":[1],"(HA)":[2],"is":[3,24,252],"an":[4,121,131],"important":[5],"biomaterial":[6],"with":[7,128,187,232],"functional":[8],"medical":[9],"and":[10,69,75,137,151,167,177,190,205,221,263],"cosmetic":[11],"applications.":[12],"As":[13],"its":[14],"synthesis":[15],"has":[16],"been":[17],"recently":[18],"reported":[19],"in":[20,250,253],"recombinant":[21,59,224],"bacteria,":[22],"it":[23],"of":[25,38,67,78,120,193,215,229,235,265],"interest":[26],"to":[27,54,94],"develop":[28],"a":[29,49,145,233,254,275],"high":[30,271],"throughput":[31,272],"screening":[32,52],"method":[33],"for":[34,56,72,179,278],"the":[35,73,79,117,155,164,172,200,203,216,222,246,259,266],"rapid":[36],"isolation":[37],"HA":[39,135,149,194,228],"accumulating":[40],"strains":[41,62,98,105,282],"transformed":[42,63],"by":[43,64,199],"combinatorial":[44],"libraries.":[45,287],"Here":[46],"we":[47],"report":[48],"novel":[50],"two-step":[51,270],"strategy":[53,277],"select":[55],"better":[57],"HA-producing":[58,97,281],"Escherichia":[60],"coli":[61],"mutation":[65,286],"libraries":[66],"rpoD":[68,248],"rpoS,":[70],"coding":[71],"sigma(D)":[74],"sigma(S)":[76],"factors":[77],"RNA":[80],"polymerase,":[81],"respectively.":[82],"The":[83,111,141],"first":[84],"screen,":[85],"based":[86,115],"on":[87,99,116],"translucent":[88,161,169],"colony":[89,109],"morphology":[90],"identification,":[91],"was":[92,114],"used":[93],"qualitatively":[95],"distinguish":[96],"agar":[100],"plates":[101],"from":[102,163,171,202,283],"non-HA":[103],"producing":[104],"that":[106,126,213,245,257],"exhibit":[107],"dense":[108],"morphology.":[110],"second":[112],"screen":[113,201,218,273],"photometric":[118],"measurement":[119],"alcian":[122,138,156],"blue":[123,139,157],"staining":[124],"solution":[125],"precipitates":[127],"HA,":[129],"creating":[130],"inverse":[132],"relationship":[133],"between":[134,148],"concentration":[136,150],"absorbance.":[140,153],"color":[142],"attenuation":[143],"fitted":[144],"second-order":[146],"polynomial":[147],"OD(540)":[152],"Using":[154],"absorbance":[158],"quantification,":[159],"74":[160],"colonies":[162,170],"HA-rpoD":[165,204],"library":[166,174,217],"78":[168],"HA-rpoS":[173,206],"were":[175,197,219],"isolated":[176],"cultured":[178],"optimal":[180],"strain":[181],"selection.":[182],"Three":[183],"representative":[184],"superior":[185,223,280],"recombinants":[186],"high,":[188],"medium":[189],"low":[191],"increase":[192],"accumulation,":[195],"respectively,":[196],"identified":[198],"mutant":[207,247],"library.":[208],"Further":[209],"flask":[210],"culture":[211],"confirmed":[212],"results":[214,243],"reliable":[220],"D72":[225,251],"highly":[226],"accumulated":[227],"561.4":[230],"mg/L":[231],"productivity":[234],"approximately":[236],"265":[237],"mg":[238],"HA/g":[239],"dry":[240],"cell.":[241],"Sequencing":[242],"showed":[244],"gene":[249],"truncated":[255],"protein":[256],"lacks":[258],"conserved":[260],"regions":[261],"3":[262],"4":[264],"sigma(D).":[267],"Generally,":[268],"this":[269],"presents":[274],"promising":[276],"selecting":[279],"large":[284],"scale":[285]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}
