{"id":"https://openalex.org/W207366351","doi":null,"title":"Development of a rapid, highly sensitive, non-radioactive assay system for hematopoietic growth factors.","display_name":"Development of a rapid, highly sensitive, non-radioactive assay system for hematopoietic growth factors.","publication_year":1991,"publication_date":"1991-12-01","ids":{"openalex":"https://openalex.org/W207366351","mag":"207366351","pmid":"https://pubmed.ncbi.nlm.nih.gov/1801697"},"language":"en","primary_location":{"id":"pmid:1801697","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1801697","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Behring Institute Mitteilungen","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044693256","display_name":"P Hintz-Obertreis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210105121","display_name":"Behringwerke (Germany)","ror":"https://ror.org/01647vc39","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210105121"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"P Hintz-Obertreis","raw_affiliation_strings":["Research Laboratories of Behringwerke AG, Marburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories of Behringwerke AG, Marburg, Germany","institution_ids":["https://openalex.org/I4210105121"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083653204","display_name":"Dorothee Krumwieh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Krumwieh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5108344772","display_name":"Friedrich Robert Seiler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F R Seiler","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.00957723,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":"90","first_page":"99","last_page":"103"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.7860999703407288,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.7860999703407288,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.07779999822378159,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoietic-growth-factors","display_name":"hematopoietic growth factors","score":0.9049999713897705},{"id":"https://openalex.org/keywords/fluorometric-assay","display_name":"fluorometric assay","score":0.9089999794960022},{"id":"https://openalex.org/keywords/recombinant-gm-csf","display_name":"recombinant GM-CSF","score":0.8949999809265137},{"id":"https://openalex.org/keywords/interleukin-3","display_name":"interleukin-3","score":0.8859999775886536},{"id":"https://openalex.org/keywords/erythropoietin","display_name":"erythropoietin","score":0.9789999723434448},{"id":"https://openalex.org/keywords/stem-cell-factor","display_name":"stem cell factor","score":0.9950000047683716}],"concepts":[{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.697458803653717},{"id":"https://openalex.org/C2778534260","wikidata":"https://www.wikidata.org/wiki/Q218706","display_name":"Erythropoietin","level":2,"score":0.6425310373306274},{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.636702835559845},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6178421378135681},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.562984049320221},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.5134862065315247},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49985671043395996},{"id":"https://openalex.org/C2775960820","wikidata":"https://www.wikidata.org/wiki/Q408378","display_name":"Growth factor","level":3,"score":0.4752112329006195},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4720885455608368},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4113420248031616},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.32701796293258667},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3111375570297241},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26357147097587585},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.17144989967346191},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.15941157937049866},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.07040777802467346},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007377","descriptor_name":"Interleukin-3","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D007377","descriptor_name":"Interleukin-3","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007938","descriptor_name":"Leukemia","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016178","descriptor_name":"Granulocyte-Macrophage Colony-Stimulating Factor","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D016178","descriptor_name":"Granulocyte-Macrophage Colony-Stimulating Factor","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:1801697","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1801697","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Behring Institute Mitteilungen","raw_type":"Journal Article"},{"id":"mag:207366351","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1801697","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2053422110","https://openalex.org/W4388386295","https://openalex.org/W1608387152","https://openalex.org/W2043811909","https://openalex.org/W2425269705","https://openalex.org/W4388386313","https://openalex.org/W2044259329","https://openalex.org/W2079868164","https://openalex.org/W2085331944","https://openalex.org/W1967102353"],"abstract_inverted_index":{"The":[0,13,63,182,192],"aim":[1],"of":[2,28,33,60,194],"this":[3],"study":[4],"was":[5,23,154],"to":[6,57,68,74,78,90],"develop":[7],"non-radioactive":[8],"cell":[9,16,65,84],"line":[10,17,66],"proliferation":[11,105,196],"assays.":[12,206],"human":[14,35],"leukemic":[15],"TF1":[18,64,110],"(Kitamura":[19],"et":[20],"al.,":[21],"1989)":[22],"used":[24],"for":[25,99,150],"the":[26,29,58,104,138,195,199,203],"determination":[27],"specific":[30],"biological":[31],"activity":[32],"recombinant":[34],"(rhu)":[36],"granulocyte-macrophage":[37],"colony-stimulating":[38],"factor":[39,86],"(GM-CSF)":[40],"and":[41,49,73,82,129,145,163,176],"rhu":[42,69,71,79,91,97,120,123],"Interleukin":[43],"3":[44],"(IL-3)":[45],"by":[46],"a":[47,54,75,157,171,177],"simple":[48],"economical":[50],"fluorometric":[51],"assay":[52,106,184],"with":[53,114,143,148],"sensitivity":[55,193],"similar":[56],"measurement":[59],"3H-thymidine":[61],"uptake.":[62],"responds":[67],"IL-3,":[70],"GM-CSF":[72,98,121],"lesser":[76],"extent":[77],"Erythropoietin":[80],"(EPO)":[81],"mast":[83],"growth":[85],"(MGF),":[87],"but":[88],"not":[89],"G-CSF.":[92],"It":[93],"is":[94],"dependent":[95],"upon":[96],"survival":[100],"in":[101,132,198],"culture.":[102],"For":[103],"1":[107],"x":[108],"10(4)":[109],"cells":[111,139],"were":[112,140,146,165],"incubated":[113,147],"20":[115],"ng":[116],"-":[117],"0.256":[118],"pg":[119],"or":[122],"IL-3":[124],"at":[125],"37":[126],"degrees":[127],"C":[128],"5%":[130],"CO2":[131],"humidified":[133],"atmosphere.":[134],"After":[135],"48":[136],"h":[137],"washed":[141],"twice":[142],"PBS":[144],"4-Methylumbelliferyl-heptanoate":[149],"60":[151],"min.":[152],"Fluorescence":[153],"determined":[155],"on":[156],"Titertek":[158],"Fluoroskan":[159],"II":[160],"(Flow":[161],"Lab.),":[162],"results":[164],"given":[166],"as":[167,202],"fluorescence":[168],"units":[169],"using":[170],"355":[172],"nm":[173,179],"excitation":[174],"filter":[175],"480":[178],"emission":[180],"filter.":[181],"developed":[183],"showed":[185],"an":[186],"interassay":[187],"variability":[188],"lower":[189],"than":[190],"15%.":[191],"assays":[197],"same":[200],"range":[201],"thymidine":[204],"incorporation":[205]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}