{"id":"https://openalex.org/W2073158293","doi":"https://doi.org/10.1074/jbc.m204970200","title":"Phosphorylation of the Catalytic Subunit of Protein Kinase A","display_name":"Phosphorylation of the Catalytic Subunit of Protein Kinase A","publication_year":2002,"publication_date":"2002-11-28","ids":{"openalex":"https://openalex.org/W2073158293","doi":"https://doi.org/10.1074/jbc.m204970200","mag":"2073158293","pmid":"https://pubmed.ncbi.nlm.nih.gov/12372837"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m204970200","doi":"https://doi.org/10.1074/jbc.m204970200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m204970200","pdf_url":"http://www.jbc.org/article/S0021925819715324/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925819715324/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061396000","display_name":"Michael J. Moore","orcid":"https://orcid.org/0000-0003-2358-083X","observed_orcids":["https://orcid.org/0000-0003-2358-083X"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael J. Moore","raw_affiliation_strings":["Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654, USA","From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"]},{"raw_affiliation_string":"From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030576537","display_name":"Joan R. Kanter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joan R. Kanter","raw_affiliation_strings":["From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019272128","display_name":"Kristian Jones","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K.C. Jones","raw_affiliation_strings":["From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045962448","display_name":"Susan Serota Taylor","orcid":"https://orcid.org/0000-0002-7702-6108","observed_orcids":["https://orcid.org/0000-0002-7702-6108"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Susan S. Taylor","raw_affiliation_strings":["From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0654","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5045962448"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I36258959"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.9524,"has_fulltext":true,"cited_by_count":113,"citation_normalized_percentile":{"value":0.85715004,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"277","issue":"49","first_page":"47878","last_page":"47884"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.8159999847412109},{"id":"https://openalex.org/keywords/catalytic-subunit","display_name":"catalytic subunit","score":0.8809999823570251},{"id":"https://openalex.org/keywords/pdpk1","display_name":"PDPK1","score":0.9129999876022339},{"id":"https://openalex.org/keywords/autophosphorylation","display_name":"autophosphorylation","score":0.8560000061988831},{"id":"https://openalex.org/keywords/activation-segment","display_name":"activation segment","score":0.800000011920929},{"id":"https://openalex.org/keywords/agc-kinase-family","display_name":"AGC kinase family","score":0.7760000228881836},{"id":"https://openalex.org/keywords/kinase-activation","display_name":"kinase activation","score":0.5009999871253967},{"id":"https://openalex.org/keywords/substrate-recognition","display_name":"substrate recognition","score":0.45899999141693115},{"id":"https://openalex.org/keywords/cos-7-cells","display_name":"COS-7 cells","score":0.625}],"concepts":[{"id":"https://openalex.org/C150109051","wikidata":"https://www.wikidata.org/wiki/Q3630494","display_name":"Autophosphorylation","level":4,"score":0.9457788467407227},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.7722964286804199},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5773154497146606},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5558034777641296},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.4899138808250427},{"id":"https://openalex.org/C159479382","wikidata":"https://www.wikidata.org/wiki/Q18030801","display_name":"MAP2K7","level":5,"score":0.4785197973251343},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.47642645239830017},{"id":"https://openalex.org/C104551158","wikidata":"https://www.wikidata.org/wiki/Q7187581","display_name":"Phosphorylation cascade","level":5,"score":0.47117310762405396},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4640452265739441},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.39262792468070984},{"id":"https://openalex.org/C87325107","wikidata":"https://www.wikidata.org/wiki/Q7251493","display_name":"Protein phosphorylation","level":4,"score":0.379644513130188},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3751069903373718},{"id":"https://openalex.org/C82495950","wikidata":"https://www.wikidata.org/wiki/Q14911732","display_name":"Cyclin-dependent kinase 2","level":4,"score":0.37098240852355957},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34145447611808777},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005998","descriptor_name":"Glycine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011233","descriptor_name":"Precipitin Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013912","descriptor_name":"Threonine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017346","descriptor_name":"Protein Serine-Threonine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017868","descriptor_name":"Cyclic AMP-Dependent Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017868","descriptor_name":"Cyclic AMP-Dependent Protein Kinases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019556","descriptor_name":"COS Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020033","descriptor_name":"Protein Isoforms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064413","descriptor_name":"3-Phosphoinositide-Dependent Protein Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m204970200","doi":"https://doi.org/10.1074/jbc.m204970200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m204970200","pdf_url":"http://www.jbc.org/article/S0021925819715324/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:12372837","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12372837","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m204970200","doi":"https://doi.org/10.1074/jbc.m204970200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m204970200","pdf_url":"http://www.jbc.org/article/S0021925819715324/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G361034356","display_name":null,"funder_award_id":"GM 19301","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G469625625","display_name":null,"funder_award_id":"DK-07233","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G567363909","display_name":"HEMOGLOBIN and BLOOD PROTEIN CHEMISTRY","funder_award_id":"5t32dk007233-35","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000216156","display_name":null,"funder_award_id":"GM19301","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2073158293.pdf","grobid_xml":"https://content.openalex.org/works/W2073158293.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1481438484","https://openalex.org/W1495346616","https://openalex.org/W1551027803","https://openalex.org/W1561853718","https://openalex.org/W1568303936","https://openalex.org/W1975734895","https://openalex.org/W1991349041","https://openalex.org/W1994954884","https://openalex.org/W1999163644","https://openalex.org/W2010770913","https://openalex.org/W2015956088","https://openalex.org/W2017394099","https://openalex.org/W2020257412","https://openalex.org/W2028970288","https://openalex.org/W2030720692","https://openalex.org/W2036931150","https://openalex.org/W2040650395","https://openalex.org/W2041926656","https://openalex.org/W2046525768","https://openalex.org/W2052221882","https://openalex.org/W2057069307","https://openalex.org/W2067405360","https://openalex.org/W2072870631","https://openalex.org/W2075767774","https://openalex.org/W2079791947","https://openalex.org/W2103002807","https://openalex.org/W2119630325","https://openalex.org/W2125141239","https://openalex.org/W2125493548","https://openalex.org/W2125992177","https://openalex.org/W2129742695","https://openalex.org/W2141524439","https://openalex.org/W2146157811","https://openalex.org/W2149238710","https://openalex.org/W2161999370","https://openalex.org/W4295216797"],"related_works":["https://openalex.org/W2075767774","https://openalex.org/W2009240129","https://openalex.org/W2978312721","https://openalex.org/W2019846233","https://openalex.org/W4242742089","https://openalex.org/W2007147736","https://openalex.org/W2119630325","https://openalex.org/W2046264538","https://openalex.org/W1941291890","https://openalex.org/W7098878317"],"abstract_inverted_index":{"The":[0,154],"identification":[1],"of":[2,12,16,56,65,72,80,157,184,192],"phosphoinositide-dependent":[3],"kinase-1":[4],"(PDK-1)":[5],"as":[6,23],"an":[7],"activating":[8,25],"kinase":[9,26,176],"for":[10,27,87,135,188],"members":[11],"the":[13,24,41,47,70,77,81,126,131,166,170,180],"AGC":[14],"family":[15],"kinases":[17],"has":[18,32],"led":[19],"to":[20,113],"its":[21],"implication":[22],"cAMP-dependent":[28],"protein":[29],"kinase.":[30],"It":[31],"been":[33],"established":[34],"in":[35,60,132,169,181,189],"vitro":[36,182],"that":[37,98,162],"PDK-1":[38,73,116,122,185],"can":[39],"phosphorylate":[40],"catalytic":[42],"(C)":[43],"subunit":[44],"(),":[45],"but":[46],"Escherichia":[48],"coli-expressed":[49],"C-subunit":[50],"undergoes":[51],"autophosphorylation.":[52],"To":[53,129],"assess":[54],"which":[55],"these":[57,158],"mechanisms":[58],"occurs":[59],"mammalian":[61],"cells,":[62,144],"a":[63,119,174],"set":[64],"mutations":[66,97],"was":[67,94,149],"engineered":[68],"flanking":[69],"site":[71,124],"phosphorylation,":[74,117,136],"Thr-197,":[75],"on":[76],"activation":[78],"segment":[79],"C-subunit.":[82],"Two":[83],"distinct":[84],"requirements":[85,134],"appeared":[86],"autophosphorylation":[88,163],"and":[89,102,145],"phosphorylation":[90,147,155,191],"by":[91,96],"PDK-1.":[92],"Autophosphorylation":[93],"disrupted":[95],"compromised":[99],"activity":[100],"(Thr-201":[101],"Gly-200)":[103],"or":[104],"altered":[105,115],"substrate":[106],"recognition":[107],"(Arg-194).":[108],"Conversely,":[109],"only":[110],"residues":[111],"peripheral":[112],"Thr-197":[114],"including":[118],"potential":[120],"hydrophobic":[121],"binding":[123],"at":[125],"C":[127],"terminus.":[128],"address":[130],"vivo":[133,190],"select":[137],"mutant":[138,159],"proteins":[139,160],"were":[140],"transfected":[141],"into":[142],"COS-7":[143],"their":[146],"state":[148],"assessed":[150],"with":[151,177],"phospho-specific":[152],"antibodies.":[153],"pattern":[156],"indicates":[161],"is":[164,186],"not":[165],"maturation":[167],"mechanism":[168],"eukaryotic":[171],"cell;":[172],"instead,":[173],"heterologous":[175],"properties":[178],"resembling":[179],"characteristics":[183],"responsible":[187],"PKA.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}