{"id":"https://openalex.org/W2072961449","doi":"https://doi.org/10.1093/nar/11.12.3959","title":"Detection of non-homology-containing heteroduplex molecules","display_name":"Detection of non-homology-containing heteroduplex molecules","publication_year":1983,"publication_date":"1983-01-01","ids":{"openalex":"https://openalex.org/W2072961449","doi":"https://doi.org/10.1093/nar/11.12.3959","mag":"2072961449","pmid":"https://pubmed.ncbi.nlm.nih.gov/6223275","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/326018"},"language":"en","primary_location":{"id":"doi:10.1093/nar/11.12.3959","doi":"https://doi.org/10.1093/nar/11.12.3959","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/11.12.3959","pdf_url":"https://academic.oup.com/nar/article-pdf/11/12/3959/4462461/11-12-3959.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/nar/article-pdf/11/12/3959/4462461/11-12-3959.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090586678","display_name":"Michael Lichten","orcid":"https://orcid.org/0000-0001-9707-2956","observed_orcids":["https://orcid.org/0000-0001-9707-2956"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael J. Lichten","raw_affiliation_strings":["Massachusetts Institute of Technology, Department of Biology 77 Massachusetts Avenue, Cambridge, MA 02139, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts Institute of Technology, Department of Biology 77 Massachusetts Avenue, Cambridge, MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":null,"display_name":"Maurice S. Fox","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maurice S. Fox","raw_affiliation_strings":["Massachusetts Institute of Technology, Department of Biology 77 Massachusetts Avenue, Cambridge, MA 02139, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts Institute of Technology, Department of Biology 77 Massachusetts Avenue, Cambridge, MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":0.4006,"has_fulltext":true,"cited_by_count":26,"citation_normalized_percentile":{"value":0.53247533,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"11","issue":"12","first_page":"3959","last_page":"3971"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.46799999475479126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.46799999475479126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.35910001397132874,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.05299999937415123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heteroduplex-dna","display_name":"heteroduplex DNA","score":0.9369999766349792},{"id":"https://openalex.org/keywords/electrophoretic-mobility","display_name":"electrophoretic mobility","score":0.753000020980835},{"id":"https://openalex.org/keywords/single-stranded-dna","display_name":"single-stranded DNA","score":0.8579999804496765}],"concepts":[{"id":"https://openalex.org/C134494468","wikidata":"https://www.wikidata.org/wiki/Q408140","display_name":"Heteroduplex","level":3,"score":0.993944525718689},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.881270170211792},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6831002235412598},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.6510881781578064},{"id":"https://openalex.org/C165525559","wikidata":"https://www.wikidata.org/wiki/Q224180","display_name":"Homology (biology)","level":3,"score":0.6484217643737793},{"id":"https://openalex.org/C54871578","wikidata":"https://www.wikidata.org/wiki/Q2074275","display_name":"Nucleic acid thermodynamics","level":4,"score":0.6157004237174988},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6064572334289551},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5508478283882141},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5052462220191956},{"id":"https://openalex.org/C2778954975","wikidata":"https://www.wikidata.org/wiki/Q143874","display_name":"Nitrocellulose","level":3,"score":0.47831466794013977},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2960517108440399},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.26171934604644775},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010582","descriptor_name":"Bacteriophage lambda","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/nar/11.12.3959","doi":"https://doi.org/10.1093/nar/11.12.3959","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/11.12.3959","pdf_url":"https://academic.oup.com/nar/article-pdf/11/12/3959/4462461/11-12-3959.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:326018","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/326018","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:6223275","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6223275","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1093/nar/11.12.3959","doi":"https://doi.org/10.1093/nar/11.12.3959","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/11.12.3959","pdf_url":"https://academic.oup.com/nar/article-pdf/11/12/3959/4462461/11-12-3959.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320319524","display_name":"Johnson and Johnson Foundation","ror":"https://ror.org/0453bzg44"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2072961449.pdf","grobid_xml":"https://content.openalex.org/works/W2072961449.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W252282098","https://openalex.org/W588812189","https://openalex.org/W1489399443","https://openalex.org/W1644129319","https://openalex.org/W1776288360","https://openalex.org/W1964876650","https://openalex.org/W1979197786","https://openalex.org/W1980108172","https://openalex.org/W2003216620","https://openalex.org/W2004845631","https://openalex.org/W2005463030","https://openalex.org/W2021272316","https://openalex.org/W2029987769","https://openalex.org/W2043860143","https://openalex.org/W2063450941","https://openalex.org/W2066373626","https://openalex.org/W2067698669","https://openalex.org/W2081885922","https://openalex.org/W2098458121","https://openalex.org/W2114408705","https://openalex.org/W2170852720","https://openalex.org/W2323778292"],"related_works":["https://openalex.org/W2007254861","https://openalex.org/W2077017381","https://openalex.org/W4229835820","https://openalex.org/W1967931877","https://openalex.org/W1905316948","https://openalex.org/W2424567554","https://openalex.org/W2063635837","https://openalex.org/W2412745074","https://openalex.org/W2560531308","https://openalex.org/W2732709841"],"abstract_inverted_index":{"Heteroduplex":[0],"DNA":[1,68],"molecules":[2],"which":[3,69],"contain":[4],"both":[5,28],"the":[6,29,38,73,77],"wild-type":[7,30],"and":[8,22,31,55],"mutant":[9],"sequences":[10,74],"of":[11,37,43],"a":[12,16],"deletion":[13,78],"nonhomology":[14],"possess":[15],"characteristic":[17],"electrophoretic":[18],"mobility":[19],"in":[20,76],"agarose":[21],"can":[23,58],"be":[24,59],"readily":[25],"separated":[26],"from":[27],"deletion-containing":[32,45],"parental":[33],"homoduplex":[34],"fragments.":[35],"Because":[36],"partial":[39],"single":[40],"stranded":[41],"character":[42],"these":[44],"heteroduplex":[46],"molecules,":[47],"they":[48],"are":[49],"selectively":[50,60],"bound":[51],"to":[52,72],"nitrocellulose":[53],"filters,":[54],"once":[56],"bound,":[57],"detected":[61],"by":[62],"hybridization":[63],"with":[64],"radioactively":[65],"labeled":[66],"single-stranded":[67],"is":[70],"homologous":[71],"absent":[75],"mutation.":[79]},"counts_by_year":[{"year":2022,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}