{"id":"https://openalex.org/W2072898799","doi":"https://doi.org/10.1006/abio.2001.5042","title":"Determination of the Relative Expression Levels of Rubisco Small Subunit Genes in Arabidopsis by Rapid Amplification of cDNA Ends","display_name":"Determination of the Relative Expression Levels of Rubisco Small Subunit Genes in Arabidopsis by Rapid Amplification of cDNA Ends","publication_year":2001,"publication_date":"2001-04-01","ids":{"openalex":"https://openalex.org/W2072898799","doi":"https://doi.org/10.1006/abio.2001.5042","mag":"2072898799","pmid":"https://pubmed.ncbi.nlm.nih.gov/11401297"},"language":"en","primary_location":{"id":"doi:10.1006/abio.2001.5042","doi":"https://doi.org/10.1006/abio.2001.5042","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.2001.5042","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045158370","display_name":"Minsoo Yoon","orcid":"https://orcid.org/0000-0001-5524-3972","observed_orcids":["https://orcid.org/0000-0001-5524-3972"]},"institutions":[{"id":"https://openalex.org/I1320288732","display_name":"Plant & Food Research","ror":"https://ror.org/02bchch95","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I4405260297"]},{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]}],"countries":["NZ"],"is_corresponding":true,"raw_author_name":"Minsoo Yoon","raw_affiliation_strings":["School of Biological Sciences, University of Auckland, Auckland, New Zealand. m.yoon@auckland.ac.nz","School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand, Private Bag 92169, Auckland, New Zealand","University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, University of Auckland, Auckland, New Zealand. m.yoon@auckland.ac.nz","institution_ids":["https://openalex.org/I154130895"]},{"raw_affiliation_string":"School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand, Private Bag 92169, Auckland, New Zealand","institution_ids":["https://openalex.org/I154130895"]},{"raw_affiliation_string":"University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand","institution_ids":["https://openalex.org/I1320288732","https://openalex.org/I154130895"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042561846","display_name":"Joanna Putterill","orcid":"https://orcid.org/0000-0003-0359-7300","observed_orcids":["https://orcid.org/0000-0003-0359-7300"]},"institutions":[{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Joanna J. Putterill","raw_affiliation_strings":["School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand, Private Bag 92169, Auckland, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand, Private Bag 92169, Auckland, New Zealand","institution_ids":["https://openalex.org/I154130895"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111814427","display_name":"Gavin S. Ross","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1320288732","display_name":"Plant & Food Research","ror":"https://ror.org/02bchch95","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I4405260297"]},{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Gavin S. Ross","raw_affiliation_strings":["University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand","institution_ids":["https://openalex.org/I1320288732","https://openalex.org/I154130895"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067750710","display_name":"William A. Laing","orcid":"https://orcid.org/0000-0003-0458-6590","observed_orcids":["https://orcid.org/0000-0003-0458-6590"]},"institutions":[{"id":"https://openalex.org/I1320288732","display_name":"Plant & Food Research","ror":"https://ror.org/02bchch95","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I4405260297"]},{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"William A. Laing","raw_affiliation_strings":["University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Auckland, Private Bag 92019, Auckland, New Zealand, The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 92169, Auckland, New Zealand","institution_ids":["https://openalex.org/I1320288732","https://openalex.org/I154130895"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5045158370"],"corresponding_institution_ids":["https://openalex.org/I1320288732","https://openalex.org/I154130895"],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.2885,"has_fulltext":false,"cited_by_count":36,"citation_normalized_percentile":{"value":0.48931724,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"291","issue":"2","first_page":"237","last_page":"244"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.00839999970048666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rubisco","display_name":"Rubisco","score":0.8830000162124634},{"id":"https://openalex.org/keywords/gene-family","display_name":"gene family","score":0.7570000290870667},{"id":"https://openalex.org/keywords/relative-gene-expression","display_name":"relative gene expression","score":0.7829999923706055},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.9169999957084656},{"id":"https://openalex.org/keywords/thermal-adaptation","display_name":"thermal adaptation","score":0.7649999856948853},{"id":"https://openalex.org/keywords/mrna-levels","display_name":"mRNA levels","score":0.6069999933242798},{"id":"https://openalex.org/keywords/gene-family-expression","display_name":"gene family expression","score":0.6050000190734863}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8202941417694092},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.7981986999511719},{"id":"https://openalex.org/C50212798","wikidata":"https://www.wikidata.org/wiki/Q422516","display_name":"RuBisCO","level":3,"score":0.7854025959968567},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.7833179235458374},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.7712223529815674},{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.6216743588447571},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5966362953186035},{"id":"https://openalex.org/C50724178","wikidata":"https://www.wikidata.org/wiki/Q381434","display_name":"Rapid amplification of cDNA ends","level":5,"score":0.5908917188644409},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5744773149490356},{"id":"https://openalex.org/C5911399","wikidata":"https://www.wikidata.org/wiki/Q2278983","display_name":"Gene family","level":4,"score":0.49424126744270325},{"id":"https://openalex.org/C103549609","wikidata":"https://www.wikidata.org/wiki/Q7575017","display_name":"Specificity factor","level":5,"score":0.4261651039123535},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.4180023670196533},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3793601989746094},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.14591005444526672},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.10687321424484253},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.08117365837097168}],"mesh":[{"descriptor_ui":"D001345","descriptor_name":"Autoradiography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012273","descriptor_name":"Ribulose-Bisphosphate Carboxylase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015971","descriptor_name":"Gene Expression Regulation, Enzymologic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018506","descriptor_name":"Gene Expression Regulation, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1006/abio.2001.5042","doi":"https://doi.org/10.1006/abio.2001.5042","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.2001.5042","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:11401297","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11401297","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320006","display_name":"Royal Society","ror":"https://ror.org/03wnrjx87"},{"id":"https://openalex.org/F4320320774","display_name":"Royal Society Te Ap\u0101rangi","ror":"https://ror.org/04tajb587"},{"id":"https://openalex.org/F4320335369","display_name":"Marsden Fund","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W114183858","https://openalex.org/W1531689573","https://openalex.org/W1569955072","https://openalex.org/W1978368163","https://openalex.org/W2010888033","https://openalex.org/W2034408935","https://openalex.org/W2062224442","https://openalex.org/W2078313792","https://openalex.org/W2094763771","https://openalex.org/W2122669307","https://openalex.org/W2147519615","https://openalex.org/W2176733407","https://openalex.org/W2267405920","https://openalex.org/W2915880292","https://openalex.org/W4213387068"],"related_works":["https://openalex.org/W7036048343","https://openalex.org/W4296777908","https://openalex.org/W2059696668","https://openalex.org/W27285536","https://openalex.org/W2055306919","https://openalex.org/W2149769161","https://openalex.org/W2053863465","https://openalex.org/W2796929697","https://openalex.org/W561850548","https://openalex.org/W2078313792"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}