{"id":"https://openalex.org/W2072567587","doi":"https://doi.org/10.1080/07391102.2012.10507410","title":"Unveiling the Unfolding Pathway of F5F8D Disorder-Associated D81H/V100D Mutant of MCFD2viaMultiple Molecular Dynamics Simulations","display_name":"Unveiling the Unfolding Pathway of F5F8D Disorder-Associated D81H/V100D Mutant of MCFD2viaMultiple Molecular Dynamics Simulations","publication_year":2012,"publication_date":"2012-02-01","ids":{"openalex":"https://openalex.org/W2072567587","doi":"https://doi.org/10.1080/07391102.2012.10507410","mag":"2072567587","pmid":"https://pubmed.ncbi.nlm.nih.gov/22208273"},"language":"en","primary_location":{"id":"doi:10.1080/07391102.2012.10507410","doi":"https://doi.org/10.1080/07391102.2012.10507410","is_oa":false,"landing_page_url":"https://doi.org/10.1080/07391102.2012.10507410","pdf_url":null,"source":{"id":"https://openalex.org/S178711970","display_name":"Journal of Biomolecular Structure and Dynamics","issn_l":"0739-1102","issn":["0739-1102","1538-0254"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biomolecular Structure and Dynamics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015099764","display_name":"Adel Hamza","orcid":"https://orcid.org/0000-0003-1148-0356","observed_orcids":["https://orcid.org/0000-0003-1148-0356"]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adel Hamza","raw_affiliation_strings":["Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, USA. ahamz3@uky.edu","Department of Pharmaceutical Sciences , College of Pharmacy, University of Kentucky , 789 South Limestone Street,  Lexington ,  Kentucky ,  40536"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, USA. ahamz3@uky.edu","institution_ids":["https://openalex.org/I143302722"]},{"raw_affiliation_string":"Department of Pharmaceutical Sciences , College of Pharmacy, University of Kentucky , 789 South Limestone Street,  Lexington ,  Kentucky ,  40536","institution_ids":["https://openalex.org/I143302722"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101761535","display_name":"Ning-Ning Wei","orcid":"https://orcid.org/0000-0001-7864-6504","observed_orcids":["https://orcid.org/0000-0001-7864-6504","https://orcid.org/0000-0003-4344-5336"]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]},{"id":"https://openalex.org/I27357992","display_name":"Dalian University of Technology","ror":"https://ror.org/023hj5876","country_code":"CN","type":"education","lineage":["https://openalex.org/I27357992"]},{"id":"https://openalex.org/I4391767660","display_name":"State Key Laboratory of Fine Chemicals","ror":"https://ror.org/03j33bp92","country_code":"CN","type":"facility","lineage":["https://openalex.org/I27357992","https://openalex.org/I4391767660"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Ning-Ning Wei","raw_affiliation_strings":["Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, 40536 ; State Key Laboratory of Fine Chemicals, School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China","Department of Pharmaceutical Sciences , College of Pharmacy, University of Kentucky , 789 South Limestone Street,  Lexington ,  Kentucky ,  40536","State Key Laboratory of Fine Chemicals , School of Life Science and Biotechnology, Dalian University of Technology ,  Dalian ,  116024 ,  China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, 40536 ; State Key Laboratory of Fine Chemicals, School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China","institution_ids":["https://openalex.org/I143302722","https://openalex.org/I27357992","https://openalex.org/I4391767660"]},{"raw_affiliation_string":"Department of Pharmaceutical Sciences , College of Pharmacy, University of Kentucky , 789 South Limestone Street,  Lexington ,  Kentucky ,  40536","institution_ids":["https://openalex.org/I143302722"]},{"raw_affiliation_string":"State Key Laboratory of Fine Chemicals , School of Life Science and Biotechnology, Dalian University of Technology ,  Dalian ,  116024 ,  China","institution_ids":["https://openalex.org/I27357992","https://openalex.org/I4391767660"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063974151","display_name":"Trudy J Scalise","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1290523313","display_name":"Magyar Agr\u00e1r- \u00e9s \u00c9lettudom\u00e1nyi Egyetem","ror":"https://ror.org/01394d192","country_code":"HU","type":"education","lineage":["https://openalex.org/I1290523313"]}],"countries":["HU"],"is_corresponding":false,"raw_author_name":"Trudy Johnson-Scalise","raw_affiliation_strings":["Laboratory of Neuro-Immuno-Endocrinology, Department of Physiology and Biochemistry, Szent Istvan University, Budapest, Hungary"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Neuro-Immuno-Endocrinology, Department of Physiology and Biochemistry, Szent Istvan University, Budapest, Hungary","institution_ids":["https://openalex.org/I1290523313"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030184373","display_name":"Frederick Nicholas Naftolin","orcid":"https://orcid.org/0000-0002-9447-6266","observed_orcids":["https://orcid.org/0000-0002-9447-6266"]},"institutions":[{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Frederick Naftolin","raw_affiliation_strings":["Department of Obstetrics and Gynecology, New York University Medical Center, New York, New York"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, New York University Medical Center, New York, New York","institution_ids":["https://openalex.org/I4210086933","https://openalex.org/I57206974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101423285","display_name":"Hoon Cho","orcid":"https://orcid.org/0000-0002-6475-5184","observed_orcids":["https://orcid.org/0000-0002-6475-5184"]},"institutions":[{"id":"https://openalex.org/I152238500","display_name":"Chosun University","ror":"https://ror.org/01zt9a375","country_code":"KR","type":"education","lineage":["https://openalex.org/I152238500"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Hoon Cho","raw_affiliation_strings":["Department of Polymer Science & Engineering, Chosun University, Gwangju, 501-759, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Polymer Science & Engineering, Chosun University, Gwangju, 501-759, South Korea","institution_ids":["https://openalex.org/I152238500"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100676088","display_name":"Chang\u2010Guo Zhan","orcid":"https://orcid.org/0000-0002-4128-7269","observed_orcids":["https://orcid.org/0000-0002-4128-7269"]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chang-Guo Zhan","raw_affiliation_strings":["Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, 40536"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, Kentucky, 40536","institution_ids":["https://openalex.org/I143302722"]}]}],"institutions":[],"countries_distinct_count":4,"institutions_distinct_count":7,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4814,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.59793206,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"29","issue":"4","first_page":"699","last_page":"714"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mcfd2","display_name":"MCFD2","score":0.9620000123977661},{"id":"https://openalex.org/keywords/lman1","display_name":"LMAN1","score":0.8989999890327454},{"id":"https://openalex.org/keywords/protein-misfolding","display_name":"protein misfolding","score":0.9409999847412109},{"id":"https://openalex.org/keywords/ef-hand-domain","display_name":"EF-hand domain","score":0.6420000195503235},{"id":"https://openalex.org/keywords/calcium-binding","display_name":"calcium binding","score":0.8799999952316284},{"id":"https://openalex.org/keywords/metal-ion-binding","display_name":"metal ion binding","score":0.47999998927116394},{"id":"https://openalex.org/keywords/protein-unfolding-pathways","display_name":"protein unfolding pathways","score":0.7590000033378601},{"id":"https://openalex.org/keywords/hydrophobic-core","display_name":"hydrophobic core","score":0.8140000104904175},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.3709999918937683}],"concepts":[{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6701304912567139},{"id":"https://openalex.org/C59593255","wikidata":"https://www.wikidata.org/wiki/Q901663","display_name":"Molecular dynamics","level":2,"score":0.6099240183830261},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5508095622062683},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5475971102714539},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4739161431789398},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.43968087434768677},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.43742918968200684},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3071311116218567},{"id":"https://openalex.org/C147597530","wikidata":"https://www.wikidata.org/wiki/Q369472","display_name":"Computational chemistry","level":1,"score":0.2536202669143677},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24099016189575195},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21154803037643433}],"mesh":[{"descriptor_ui":"D005169","descriptor_name":"Factor VIII","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D033921","descriptor_name":"Vesicular Transport Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D037241","descriptor_name":"Mannose-Binding Lectins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1080/07391102.2012.10507410","doi":"https://doi.org/10.1080/07391102.2012.10507410","is_oa":false,"landing_page_url":"https://doi.org/10.1080/07391102.2012.10507410","pdf_url":null,"source":{"id":"https://openalex.org/S178711970","display_name":"Journal of Biomolecular Structure and Dynamics","issn_l":"0739-1102","issn":["0739-1102","1538-0254"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biomolecular Structure and Dynamics","raw_type":"journal-article"},{"id":"pmid:22208273","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22208273","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular structure & dynamics","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G6678004481","display_name":"High-activity mutants of cocaine esterase for treatment of drug addiction","funder_award_id":"R01DA025100","funder_id":"https://openalex.org/F4320337347","funder_display_name":"National Institute on Drug Abuse"}],"funders":[{"id":"https://openalex.org/F4320337347","display_name":"National Institute on Drug Abuse","ror":"https://ror.org/00fq5cm18"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":80,"referenced_works":["https://openalex.org/W1491824558","https://openalex.org/W1548718817","https://openalex.org/W1601691609","https://openalex.org/W1608506719","https://openalex.org/W1819402632","https://openalex.org/W1963051938","https://openalex.org/W1968365826","https://openalex.org/W1968702502","https://openalex.org/W1971154617","https://openalex.org/W1976993208","https://openalex.org/W1977330100","https://openalex.org/W1977428203","https://openalex.org/W1978983857","https://openalex.org/W1981462659","https://openalex.org/W1981781995","https://openalex.org/W1982277580","https://openalex.org/W1982764243","https://openalex.org/W1983951061","https://openalex.org/W1984993506","https://openalex.org/W1989893963","https://openalex.org/W1990049796","https://openalex.org/W1993414433","https://openalex.org/W1993543753","https://openalex.org/W1998540108","https://openalex.org/W2003737076","https://openalex.org/W2008708467","https://openalex.org/W2008902732","https://openalex.org/W2011276710","https://openalex.org/W2012037973","https://openalex.org/W2012911467","https://openalex.org/W2014858193","https://openalex.org/W2018363268","https://openalex.org/W2018503071","https://openalex.org/W2021040301","https://openalex.org/W2021427342","https://openalex.org/W2029636882","https://openalex.org/W2029667189","https://openalex.org/W2035266068","https://openalex.org/W2035687084","https://openalex.org/W2038823700","https://openalex.org/W2042542015","https://openalex.org/W2042707934","https://openalex.org/W2044023226","https://openalex.org/W2045999651","https://openalex.org/W2046912614","https://openalex.org/W2053872025","https://openalex.org/W2055814699","https://openalex.org/W2062203486","https://openalex.org/W2063274819","https://openalex.org/W2067244262","https://openalex.org/W2067474357","https://openalex.org/W2068857846","https://openalex.org/W2070777173","https://openalex.org/W2077141405","https://openalex.org/W2084607551","https://openalex.org/W2085006382","https://openalex.org/W2087431066","https://openalex.org/W2090833556","https://openalex.org/W2100881236","https://openalex.org/W2103420861","https://openalex.org/W2106320506","https://openalex.org/W2118056979","https://openalex.org/W2121120391","https://openalex.org/W2123768693","https://openalex.org/W2125938217","https://openalex.org/W2128572087","https://openalex.org/W2130479394","https://openalex.org/W2131037694","https://openalex.org/W2144718643","https://openalex.org/W2145692832","https://openalex.org/W2155589696","https://openalex.org/W2157271565","https://openalex.org/W2172194876","https://openalex.org/W2179033920","https://openalex.org/W2301649499","https://openalex.org/W2396076386","https://openalex.org/W2568122745","https://openalex.org/W4240267121","https://openalex.org/W4298846468","https://openalex.org/W4324262486"],"related_works":["https://openalex.org/W1702736354","https://openalex.org/W1503016440","https://openalex.org/W1976010436","https://openalex.org/W1980573861","https://openalex.org/W1998434029","https://openalex.org/W2071460764","https://openalex.org/W1982277580","https://openalex.org/W2121120391","https://openalex.org/W2792703449","https://openalex.org/W2243144269"],"abstract_inverted_index":{"Combined":[0],"factor":[1],"deficiency":[2],"(F5F8D)":[3],"is":[4,75,86,109,199,254],"a":[5,29,181],"rare":[6],"autosomal":[7],"recessive":[8],"disorder":[9],"caused":[10],"by":[11,256],"mutations":[12,68],"in":[13,105,114,165,187,216,250,269],"the":[14,47,51,65,81,90,94,102,115,118,122,132,139,142,145,148,160,166,189,193,202,209,217,224,231,238,247,251,257,263,266,270,277,284,287,300,308],"LMAN1":[15],"or":[16],"MCFD2":[17,67,107,135,221,311],"genes.":[18],"It":[19],"has":[20],"been":[21],"proposed":[22],"that":[23,138,164,201],"this":[24,185],"pathogenic":[25],"process":[26],"occurs":[27],"via":[28],"multi-step":[30],"pathway":[31,82],"involving":[32],"metal":[33,91,227],"loss,":[34],"EF-hand-Ca21":[35],"dissociation":[36],"and":[37,93,172,229,281,303],"assembly":[38],"of":[39,50,64,100,117,121,144,184,192,220,226,233,265,286],"misfolded":[40],"MCFD2-LMAN1":[41,288],"complex.":[42,289],"Here,":[43],"we":[44],"have":[45],"investigated":[46],"solution":[48],"conformations":[49],"MCFD2((D81H,V100D))":[52],"protein":[53,146,253,278,312],"mutant":[54],"through":[55],"extensive":[56],"molecular":[57,119],"dynamics":[58],"(MD)":[59],"simulations.":[60],"The":[61,241,290],"V100D,":[62],"one":[63],"many":[66],"known":[69],"to":[70,73,77,111,223,230],"be":[71,78],"associated":[72],"F5F8D,":[74],"difficult":[76],"reconciled":[79],"with":[80,126],"model":[83],"because":[84],"it":[85,198],"located":[87,206],"far":[88,207],"from":[89,295],"sites":[92],"MCFD2/LMAN1":[95],"interface.":[96],"Consequently,":[97],"an":[98],"inspection":[99],"all":[101],"steps":[103],"involved":[104],"D81H/V100D":[106],"misfolding":[108],"expected":[110],"provide":[112],"hints":[113],"understanding":[116],"basis":[120],"disease.":[123],"A":[124],"comparison":[125],"parallel":[127],"studies":[128],"carried":[129],"out":[130,137],"for":[131,147],"Wild-Type":[133],"(WT)":[134],"pointed":[136],"mutation":[140,268],"decreases":[141],"affinity":[143],"Ca21":[149],"ion.":[150],"Multiple":[151],"explicit":[152],"solvents":[153],"MD":[154],"simulations":[155,298],"(50":[156],"ns)":[157],"performed":[158],"on":[159],"two":[161],"proteins":[162],"revealed":[163],"WT":[167],"protein,":[168],"stable":[169],"H-bond":[170],"network":[171],"compact":[173],"hydrophobic":[174,243],"core":[175,219],"region":[176,186],"are":[177],"created":[178],"thus":[179],"confirming":[180],"pivotal":[182],"role":[183],"driving":[188],"biophysical":[190],"properties":[191],"entire":[194],"protein.":[195],"In":[196],"fact":[197],"shown":[200],"V100D":[203],"mutation,":[204],"although":[205],"away":[208],"EF-hand":[210],"domain,":[211],"may":[212,274],"induce":[213],"subtle":[214],"modification":[215],"structural":[218,292],"leading":[222],"loosening":[225],"binding":[228],"formation":[232,285],"metastable":[234],"intermediate":[235],"states":[236],"along":[237],"unfolding":[239,279],"pathway.":[240],"native-like":[242],"cluster":[244],"formed":[245],"near":[246],"V100":[248],"residue":[249],"wild-type":[252],"disrupted":[255],"negatively":[258],"charged":[259],"Asparagine":[260],"residue.":[261],"Furthermore,":[262],"presence":[264],"D81H":[267],"EF-1":[271],"hand":[272],"domain":[273],"also":[275],"increase":[276],"rate":[280],"consequently":[282],"prevent":[283],"detailed":[291],"insights":[293,306],"obtained":[294],"our":[296],"large-scale":[297],"complement":[299],"clinical":[301],"features":[302],"offer":[304],"useful":[305],"into":[307],"mechanism":[309],"behind":[310],"misfolding.":[313]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}