{"id":"https://openalex.org/W2072471222","doi":"https://doi.org/10.1021/bi00300a014","title":"Comparison of the physical and functional properties of the 48-subunit native molecule and the 24- and 12-subunit dissociation intermediates of Limulus polyphemus hemocyanin","display_name":"Comparison of the physical and functional properties of the 48-subunit native molecule and the 24- and 12-subunit dissociation intermediates of Limulus polyphemus hemocyanin","publication_year":1984,"publication_date":"1984-02-01","ids":{"openalex":"https://openalex.org/W2072471222","doi":"https://doi.org/10.1021/bi00300a014","mag":"2072471222","pmid":"https://pubmed.ncbi.nlm.nih.gov/25856834"},"language":"en","primary_location":{"id":"doi:10.1021/bi00300a014","doi":"https://doi.org/10.1021/bi00300a014","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00300a014","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010967667","display_name":"Michael D. Brenowitz","orcid":"https://orcid.org/0000-0003-0048-1716","observed_orcids":["https://orcid.org/0000-0003-0048-1716"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Brenowitz","raw_affiliation_strings":["Duke University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Duke University","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060269662","display_name":"Celia Bonaventura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Celia Bonaventura","raw_affiliation_strings":["Duke University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Duke University","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5107412810","display_name":"Joseph Bonaventura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph Bonaventura","raw_affiliation_strings":["Duke University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Duke University","institution_ids":["https://openalex.org/I170897317"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I170897317"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.3768,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.59255898,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":96},"biblio":{"volume":"23","issue":"5","first_page":"879","last_page":"888"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12094","display_name":"Hemoglobin structure and function","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12094","display_name":"Hemoglobin structure and function","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.014600000344216824,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/limulus-polyphemus","display_name":"Limulus polyphemus","score":0.9210000038146973},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.37700000405311584},{"id":"https://openalex.org/keywords/calcium-stabilization","display_name":"calcium stabilization","score":0.5370000004768372},{"id":"https://openalex.org/keywords/oxygen-affinity","display_name":"oxygen affinity","score":0.5809999704360962},{"id":"https://openalex.org/keywords/ph-sensitivity","display_name":"pH sensitivity","score":0.49300000071525574},{"id":"https://openalex.org/keywords/circular-dichroism","display_name":"circular dichroism","score":0.6909999847412109},{"id":"https://openalex.org/keywords/subunit-interactions","display_name":"subunit interactions","score":0.37700000405311584}],"concepts":[{"id":"https://openalex.org/C2779214863","wikidata":"https://www.wikidata.org/wiki/Q7226744","display_name":"Polyphemus","level":3,"score":0.6211652755737305},{"id":"https://openalex.org/C2776487282","wikidata":"https://www.wikidata.org/wiki/Q2104567","display_name":"Limulus","level":2,"score":0.5742359757423401},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.5562771558761597},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5089280605316162},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4544546604156494},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.453968346118927},{"id":"https://openalex.org/C94124525","wikidata":"https://www.wikidata.org/wiki/Q912550","display_name":"Categorization","level":2,"score":0.44411763548851013},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.41392406821250916},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.33504781126976013},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.332261323928833},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3223276734352112},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.2536730170249939},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.24715939164161682},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24013087153434753},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.23742517828941345},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.22106629610061646},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13670536875724792},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11902967095375061},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.11759164929389954}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006433","descriptor_name":"Hemocyanins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006737","descriptor_name":"Horseshoe Crabs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010100","descriptor_name":"Oxygen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020836","descriptor_name":"Protein Structure, Quaternary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D055503","descriptor_name":"Protein Multimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060829","descriptor_name":"Arthropod Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00300a014","doi":"https://doi.org/10.1021/bi00300a014","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00300a014","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:25856834","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25856834","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2109624233","https://openalex.org/W2054820666","https://openalex.org/W93292460","https://openalex.org/W192323771","https://openalex.org/W2006541528","https://openalex.org/W2062472660","https://openalex.org/W1643856707","https://openalex.org/W2025400334","https://openalex.org/W141315524","https://openalex.org/W2436849194"],"abstract_inverted_index":{"Limulus":[0,175,218],"hemocyanin":[1,176,219],"is":[2,7,88,143,248],"a":[3,70,75,104,144,166],"48-subunit":[4,50,62,232,245,323],"complex":[5,51,108,216,308],"that":[6,16,195,247,284,287,300,312,320,334,336],"composed":[8],"of":[9,20,26,32,96,103,117,128,161,174,217,258,267,305,332,350],"eight":[10],"immunochemically":[11],"distinct":[12,348],"subunits.Conditions":[13],"were":[14,66,199],"established":[15],"allowed":[17],"for":[18],"comparison":[19],"the":[21,27,78,91,97,101,110,115,126,149,158,191,196,209,230,244,256,260,285,298,306,318,322],"structural":[22],"and":[23,35,46,93,148,265,294,311,346],"functional":[24,42,227,236],"properties":[25,228,237],"native":[28,231],"molecule":[29],"with":[30,69,100,190,243,329],"those":[31],"its":[33,226],"24-subunit":[34,118,129,215,307],"12-subunit":[36,141],"dissociation":[37,79,111,127,134,254],"products":[38],"by":[39,58,179,270,344],"analytical":[40],"ultracentrifugation,":[41],"analysis,stopped-flow":[43],"light":[44],"scattering,":[45],"circular":[47,181],"dichroism":[48,182],"spectroscopy.The":[49],"was":[52,82,212,220],"found":[53,200,221,249],"to":[54,80,114,131,135,164,201,204,222,229,240,255],"be":[55,165,202,223,241],"specifically":[56],"stabilized":[57],"calcium":[59,76],"ions.":[60],"When":[61],"molecules":[63,119,130],"at":[64,338],"pH7":[65],"rapidly":[67,167],"mixed":[68],"pH":[71,205,293],"9":[72],"buffer":[73],"containing":[74],"chelator,":[77,98],"monomers":[81],"complete":[83],"within":[84],"minutes.":[85],"The":[86,133,170,214],"rate":[87],"dependent":[89],"upon":[90],"concentration":[92],"Ca2+":[94],"affinity":[95,269],"consistent":[99,328],"formation":[102,304],"transient":[105],"chelator-calcium":[106],"ion-protein":[107],"during":[109],"process.":[112],"Dissociation":[113],"level":[116,257],"occurs":[120],"much":[121],"more":[122],"slowly":[123],"than":[124],"does":[125],"monomers.":[132],"monomers,":[136],"from":[137,297,309,317],"48-,":[138],"24-,":[139],"or":[140],"aggregates,":[142],"highly":[145],"cooperative":[146],"process,":[147],"corresponding":[150],"reassembly":[151],"reactions":[152],"show":[153],"appreciable":[154],"hysteresis.":[155],"In":[156],"contrast,":[157],"reversible":[159],"dimerization":[160],"dodecamers":[162,289,310],"appears":[163],"equilibrating":[168],"system.":[169],"varied":[171],"aggregation":[172,210],"states":[173],"are":[177,272,290,301,327,342],"characterized":[178],"different":[180,291,340],"spectra.":[183],"These":[184,325],"are,":[185],"however,":[186],"not":[187],"unambiguously":[188],"associated":[189,242],"aggregation-state":[192],"changes":[193],"in":[194,225,251,277,292,303,314],"CD":[197],"spectra":[198],"sensitive":[203],"under":[206],"conditions":[207],"where":[208],"state":[211],"unaltered.":[213],"similar":[224],"aggregate.":[233],"No":[234],"unique":[235],"thus":[238],"appear":[239],"ensemble":[246],"only":[250],"horseshoe":[252],"crabs.Upon":[253],"dodecamers,":[259],"reverse":[261],"Bohr":[262],"effect,":[263],"cooperatively,":[264],"modulation":[266],"oxygen":[268],"NaCl":[271],"still":[273],"present":[274],"although":[275],"diminished":[276],"magnitude.Taken":[278],"together,":[279],"these":[280,313,339],"studies":[281],"clearly":[282],"indicate":[283,335],"interactions":[286,299,319,337],"stabilize":[288,321],"ion":[295],"sensitivity":[296],"involved":[302],"turn":[315],"differ":[316],"complex.":[324],"results":[326],"other":[330],"lines":[331],"evidence":[333],"levels":[341],"dominated":[343],"structurally":[345],"functionally":[347],"types":[349],"subunits.":[351]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}