{"id":"https://openalex.org/W2071624868","doi":"https://doi.org/10.1021/pr800396c","title":"Intrinsic Peptidase Activity Causes a Sequential Multi-Step Reaction (SMSR) in Digestion of Human Plasma Peptides","display_name":"Intrinsic Peptidase Activity Causes a Sequential Multi-Step Reaction (SMSR) in Digestion of Human Plasma Peptides","publication_year":2008,"publication_date":"2008-11-11","ids":{"openalex":"https://openalex.org/W2071624868","doi":"https://doi.org/10.1021/pr800396c","mag":"2071624868","pmid":"https://pubmed.ncbi.nlm.nih.gov/19367699"},"language":"en","primary_location":{"id":"doi:10.1021/pr800396c","doi":"https://doi.org/10.1021/pr800396c","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr800396c","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052437531","display_name":"Jizu Yi","orcid":"https://orcid.org/0000-0002-8976-5207","observed_orcids":["https://orcid.org/0000-0002-8976-5207"]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jizu Yi","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417, USA. Jizu_Yi@bd.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]},{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417, USA. Jizu_Yi@bd.com","institution_ids":["https://openalex.org/I146461966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100380850","display_name":"Zhaoxia Liu","orcid":"https://orcid.org/0000-0002-5661-0743","observed_orcids":["https://orcid.org/0000-0002-5661-0743"]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhaoxia Liu","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090060877","display_name":"David Craft","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Craft","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081012007","display_name":"Patrick O\u2019Mullan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrick O\u2019Mullan","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Gang Ju","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gang Ju","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007436679","display_name":"Craig A. Gelfand","orcid":"https://orcid.org/0009-0007-7349-7395","observed_orcids":["https://orcid.org/0009-0007-7349-7395"]},"institutions":[{"id":"https://openalex.org/I146461966","display_name":"Becton Dickinson (United States)","ror":"https://ror.org/048vrgr14","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Craig A. Gelfand","raw_affiliation_strings":["BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Diagnostics, and BD Medical, One Becton Drive, Franklin Lakes, New Jersey 07417","institution_ids":["https://openalex.org/I146461966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5007436679","https://openalex.org/A5052437531"],"corresponding_institution_ids":["https://openalex.org/I146461966"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.8669,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.8411384,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"7","issue":"12","first_page":"5112","last_page":"5118"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8852999806404114,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8852999806404114,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.051899999380111694,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.009800000116229057,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peptide-biomarkers","display_name":"peptide biomarkers","score":0.7609999775886536},{"id":"https://openalex.org/keywords/protease-inhibitors","display_name":"protease inhibitors","score":0.6800000071525574},{"id":"https://openalex.org/keywords/anticoagulants","display_name":"anticoagulants","score":0.8769999742507935},{"id":"https://openalex.org/keywords/edta-plasma","display_name":"EDTA plasma","score":0.6710000038146973},{"id":"https://openalex.org/keywords/citrate-plasma","display_name":"citrate plasma","score":0.921999990940094},{"id":"https://openalex.org/keywords/heparinized-plasma","display_name":"heparinized plasma","score":0.9380000233650208},{"id":"https://openalex.org/keywords/serum","display_name":"serum","score":0.4269999861717224},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.6759999990463257},{"id":"https://openalex.org/keywords/internal-control","display_name":"internal control","score":0.5460000038146973},{"id":"https://openalex.org/keywords/preanalytical-variation","display_name":"pre-analytical variability","score":0.7070000171661377}],"concepts":[{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.8218438029289246},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.8039792776107788},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7964606881141663},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5963516235351562},{"id":"https://openalex.org/C2779036427","wikidata":"https://www.wikidata.org/wiki/Q381899","display_name":"Fibrinogen","level":2,"score":0.5631515383720398},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5514397621154785},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.5491859316825867},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.5132100582122803},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5019145011901855},{"id":"https://openalex.org/C2776575606","wikidata":"https://www.wikidata.org/wiki/Q79749","display_name":"Blood plasma","level":2,"score":0.4469185173511505},{"id":"https://openalex.org/C146543888","wikidata":"https://www.wikidata.org/wiki/Q425056","display_name":"Blood proteins","level":2,"score":0.44095680117607117},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.44023117423057556},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4394564926624298},{"id":"https://openalex.org/C3020134792","wikidata":"https://www.wikidata.org/wiki/Q79749","display_name":"Human plasma","level":2,"score":0.438248872756958},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4363100826740265},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.33962953090667725},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.08565086126327515},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004492","descriptor_name":"Edetic Acid","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010949","descriptor_name":"Plasma","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011480","descriptor_name":"Protease Inhibitors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/pr800396c","doi":"https://doi.org/10.1021/pr800396c","is_oa":false,"landing_page_url":"https://doi.org/10.1021/pr800396c","pdf_url":null,"source":{"id":"https://openalex.org/S74367812","display_name":"Journal of Proteome Research","issn_l":"1535-3893","issn":["1535-3893","1535-3907"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Proteome Research","raw_type":"journal-article"},{"id":"pmid:19367699","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19367699","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of proteome research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1732176471","https://openalex.org/W1819870217","https://openalex.org/W1968534108","https://openalex.org/W1971654402","https://openalex.org/W1975348027","https://openalex.org/W1977184198","https://openalex.org/W1978694357","https://openalex.org/W1979220635","https://openalex.org/W1988370933","https://openalex.org/W1994239683","https://openalex.org/W2010780360","https://openalex.org/W2013861694","https://openalex.org/W2019102633","https://openalex.org/W2024923641","https://openalex.org/W2027424999","https://openalex.org/W2031949200","https://openalex.org/W2037871774","https://openalex.org/W2038241145","https://openalex.org/W2055118323","https://openalex.org/W2055119563","https://openalex.org/W2057356121","https://openalex.org/W2068481474","https://openalex.org/W2072347219","https://openalex.org/W2075750326","https://openalex.org/W2077094586","https://openalex.org/W2077966741","https://openalex.org/W2104786760","https://openalex.org/W2132395789","https://openalex.org/W2134389439","https://openalex.org/W2143455062","https://openalex.org/W2145957085","https://openalex.org/W2157492758","https://openalex.org/W2171081285","https://openalex.org/W2172116519","https://openalex.org/W2519438632"],"related_works":["https://openalex.org/W2407988820","https://openalex.org/W1619897426","https://openalex.org/W2620755219","https://openalex.org/W7098607953","https://openalex.org/W2040374384","https://openalex.org/W2059810423","https://openalex.org/W4403388970","https://openalex.org/W2388497299","https://openalex.org/W4390803991","https://openalex.org/W2910805740"],"abstract_inverted_index":{"Human":[0],"plasma":[1,40,68,114,125,132,201],"and":[2,7,15,41,44,60,67,94,126,135,209],"serum":[3,42,66],"samples,":[4],"including":[5],"protein":[6],"peptide":[8,30,37,51],"biomarkers,":[9],"are":[10,108],"subjected":[11],"to":[12],"preanalytical":[13,215],"variations":[14],"instability":[16],"caused":[17],"by":[18,32],"intrinsic":[19,145,190],"proteases.":[20],"In":[21],"this":[22,99],"study,":[23],"we":[24],"directly":[25],"investigated":[26],"the":[27,76,106,112,129,158,163,180,185,189],"stability":[28],"of":[29,131,157,162,179,188],"biomarkers":[31],"spiking":[33],"an":[34,148],"isotopically":[35],"labeled":[36],"into":[38,200],"human":[39],"samples":[43],"then":[45],"monitoring":[46],"its":[47,61],"time-dependent":[48],"change.":[49],"Fibrinogen":[50],"A":[52],"(FPA)":[53],"was":[54,85],"used":[55],"as":[56,75,202,213],"a":[57,64,176,214],"model":[58],"substrate,":[59],"degradation":[62],"in":[63,98,102,111,117,155,170],"conventional":[65],"either":[69],"with":[70],"citrate,":[71],"heparin,":[72],"or":[73,78],"EDTA":[74,79,120],"anticoagulant,":[77],"plus":[80],"protease":[81,136],"inhibitors":[82,137],"(inhibited":[83],"plasma),":[84],"measured":[86],"using":[87],"time-course":[88],"MALDI-TOF":[89],"MS":[90],"analysis.":[91],"The":[92],"FPA":[93],"other":[95],"peptides":[96,107,198],"tested":[97],"study":[100],"vary":[101],"these":[103],"samples.":[104],"However,":[105],"most":[109],"stable":[110],"inhibited":[113],"followed":[115],"by,":[116],"general":[118],"order,":[119],"plasma,":[121,123],"citrate":[122],"heparin":[124],"serum,":[127,134],"demonstrating":[128],"benefit":[130],"versus":[133],"for":[138],"biomarker":[139],"stabilization.":[140],"Kinetic":[141],"analysis":[142,161],"indicates":[143],"that":[144,166,196],"peptidases":[146],"cause":[147],"observed":[149],"first-order":[150],"Sequential":[151],"Multiple-Step":[152],"Reaction":[153],"(SMSR)":[154],"digestion":[156],"peptide.":[159],"Modeling":[160],"SMSR":[164],"demonstrates":[165],"step":[167],"reactions":[168],"differ":[169],"their":[171,211],"kinetic":[172],"rate":[173],"constants,":[174],"suggesting":[175],"significant":[177],"contribution":[178],"truncated":[181],"end":[182],"residue":[183],"on":[184],"substrate":[186],"specificity":[187],"peptidase(s).":[191],"Our":[192],"observations":[193],"further":[194],"show":[195],"synthetic":[197],"introduced":[199],"internal":[203],"controls":[204],"can":[205],"also":[206],"be":[207,219],"degraded,":[208],"thus,":[210],"(in)stability":[212],"variable":[216],"should":[217],"not":[218],"overlooked.":[220]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}