{"id":"https://openalex.org/W2070988320","doi":"https://doi.org/10.1016/s0921-8777(98)00031-7","title":"The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts","display_name":"The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts","publication_year":1998,"publication_date":"1998-09-01","ids":{"openalex":"https://openalex.org/W2070988320","doi":"https://doi.org/10.1016/s0921-8777(98)00031-7","mag":"2070988320","pmid":"https://pubmed.ncbi.nlm.nih.gov/9806417"},"language":"en","primary_location":{"id":"doi:10.1016/s0921-8777(98)00031-7","doi":"https://doi.org/10.1016/s0921-8777(98)00031-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0921-8777(98)00031-7","pdf_url":null,"source":{"id":"https://openalex.org/S4210192122","display_name":"Mutation Research/DNA Repair","issn_l":"0921-8777","issn":["0921-8777","1386-1476"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mutation Research/DNA Repair","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044370400","display_name":"Wenya Huang","orcid":"https://orcid.org/0000-0002-4244-0800","observed_orcids":["https://orcid.org/0000-0002-4244-0800"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wenya Huang","raw_affiliation_strings":["Department of Pathology, University of Texas, Southwestern Medical Center, Dallas 75235-9072, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas, Southwestern Medical Center, Dallas 75235-9072, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021960903","display_name":"William John Feaver","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162577319","display_name":"The University of Texas at Dallas","ror":"https://ror.org/049emcs32","country_code":"US","type":"education","lineage":["https://openalex.org/I162577319"]},{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William J. Feaver","raw_affiliation_strings":["Laboratory of Molecular Pathology, Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX 75235-9072, USA","University of Texas at Dallas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Pathology, Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX 75235-9072, USA","institution_ids":["https://openalex.org/I867280407"]},{"raw_affiliation_string":"University of Texas at Dallas","institution_ids":["https://openalex.org/I162577319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051816777","display_name":"Alan E. Tomkinson","orcid":"https://orcid.org/0000-0002-2671-1711","observed_orcids":["https://orcid.org/0000-0002-2671-1711"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alan E. Tomkinson","raw_affiliation_strings":["Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA","University of Texas Health Science Center at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA","institution_ids":["https://openalex.org/I165951966"]},{"raw_affiliation_string":"University of Texas Health Science Center at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109149946","display_name":"Errol C. Friedberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Errol C. Friedberg","raw_affiliation_strings":["Laboratory of Molecular Pathology, Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX 75235-9072, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Pathology, Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX 75235-9072, USA","institution_ids":["https://openalex.org/I867280407"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5109149946"],"corresponding_institution_ids":["https://openalex.org/I867280407"],"apc_list":null,"apc_paid":null,"fwci":0.6973,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.67296108,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"408","issue":"3","first_page":"183","last_page":"194"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleotide-excision-repair","display_name":"nucleotide excision repair","score":0.9919999837875366},{"id":"https://openalex.org/keywords/replication-protein-a","display_name":"replication protein A","score":0.9620000123977661},{"id":"https://openalex.org/keywords/proliferating-cell-nuclear-antigen","display_name":"proliferating cell nuclear antigen","score":0.9160000085830688},{"id":"https://openalex.org/keywords/rfa1","display_name":"Rfa1","score":0.8460000157356262},{"id":"https://openalex.org/keywords/pcna","display_name":"PCNA","score":0.953000009059906},{"id":"https://openalex.org/keywords/temperature-sensitive-mutants","display_name":"temperature-sensitive mutants","score":0.6480000019073486},{"id":"https://openalex.org/keywords/n-degron","display_name":"N-degron","score":0.6150000095367432},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.7239999771118164},{"id":"https://openalex.org/keywords/tfiih","display_name":"TFIIH","score":0.9010000228881836},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8930000066757202},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.7229999899864197}],"concepts":[{"id":"https://openalex.org/C34688535","wikidata":"https://www.wikidata.org/wiki/Q418732","display_name":"Proliferating cell nuclear antigen","level":3,"score":0.8267987966537476},{"id":"https://openalex.org/C104451858","wikidata":"https://www.wikidata.org/wiki/Q2714458","display_name":"Nucleotide excision repair","level":4,"score":0.7276535034179688},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6596944332122803},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.5786128044128418},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.5459240674972534},{"id":"https://openalex.org/C188036394","wikidata":"https://www.wikidata.org/wiki/Q14902224","display_name":"Replication factor C","level":5,"score":0.5099254846572876},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.45703211426734924},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.43211299180984497},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.416564404964447},{"id":"https://openalex.org/C113271649","wikidata":"https://www.wikidata.org/wiki/Q2565234","display_name":"Replication protein A","level":5,"score":0.41549673676490784},{"id":"https://openalex.org/C140248907","wikidata":"https://www.wikidata.org/wiki/Q17913449","display_name":"DDB1","level":5,"score":0.4102531671524048},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3547307848930359},{"id":"https://openalex.org/C98086919","wikidata":"https://www.wikidata.org/wiki/Q118019","display_name":"Eukaryotic DNA replication","level":4,"score":0.31451690196990967},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.29123908281326294},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2875770926475525},{"id":"https://openalex.org/C94966510","wikidata":"https://www.wikidata.org/wiki/Q2252764","display_name":"DNA-binding protein","level":4,"score":0.2301601767539978},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.2270568609237671}],"mesh":[{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012319","descriptor_name":"RNA Polymerase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014157","descriptor_name":"Transcription Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014466","descriptor_name":"Ultraviolet Rays","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018809","descriptor_name":"Proliferating Cell Nuclear Antigen","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018809","descriptor_name":"Proliferating Cell Nuclear Antigen","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D019473","descriptor_name":"Transcription Factors, TFII","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D035362","descriptor_name":"Transcription Factor TFIID","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D035381","descriptor_name":"TATA-Binding Protein Associated Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051716","descriptor_name":"Replication Protein A","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051758","descriptor_name":"Transcription Factor TFIIH","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0921-8777(98)00031-7","doi":"https://doi.org/10.1016/s0921-8777(98)00031-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0921-8777(98)00031-7","pdf_url":null,"source":{"id":"https://openalex.org/S4210192122","display_name":"Mutation Research/DNA Repair","issn_l":"0921-8777","issn":["0921-8777","1386-1476"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mutation Research/DNA Repair","raw_type":"journal-article"},{"id":"pmid:9806417","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9806417","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mutation research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4527253917","display_name":null,"funder_award_id":"CA12420","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306279","display_name":"Jane Coffin Childs Memorial Fund for Medical Research","ror":"https://ror.org/04yhme281"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W1491876634","https://openalex.org/W1538598346","https://openalex.org/W1550656265","https://openalex.org/W1899289062","https://openalex.org/W1933350980","https://openalex.org/W1967902178","https://openalex.org/W1979781336","https://openalex.org/W1982034145","https://openalex.org/W1985084999","https://openalex.org/W1992082799","https://openalex.org/W1992179456","https://openalex.org/W1995806503","https://openalex.org/W2004669805","https://openalex.org/W2035746057","https://openalex.org/W2039068784","https://openalex.org/W2040150820","https://openalex.org/W2040779655","https://openalex.org/W2042722733","https://openalex.org/W2048599311","https://openalex.org/W2060899717","https://openalex.org/W2075716846","https://openalex.org/W2093595958","https://openalex.org/W2098711782","https://openalex.org/W2123985521","https://openalex.org/W2139768549","https://openalex.org/W2163864783"],"related_works":["https://openalex.org/W2040779655","https://openalex.org/W2042722733","https://openalex.org/W2033110660","https://openalex.org/W2105142279","https://openalex.org/W2060899717","https://openalex.org/W2115637380","https://openalex.org/W2165024229","https://openalex.org/W1567770229","https://openalex.org/W2560513662","https://openalex.org/W2137605101"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}