{"id":"https://openalex.org/W2070189028","doi":"https://doi.org/10.1021/ja0350684","title":"Evaluation of Backbone Proton Positions and Dynamics in a Small Protein by Liquid Crystal NMR Spectroscopy","display_name":"Evaluation of Backbone Proton Positions and Dynamics in a Small Protein by Liquid Crystal NMR Spectroscopy","publication_year":2003,"publication_date":"2003-07-01","ids":{"openalex":"https://openalex.org/W2070189028","doi":"https://doi.org/10.1021/ja0350684","mag":"2070189028","pmid":"https://pubmed.ncbi.nlm.nih.gov/15369375"},"language":"en","primary_location":{"id":"doi:10.1021/ja0350684","doi":"https://doi.org/10.1021/ja0350684","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja0350684","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028480003","display_name":"Tobias S. Ulmer","orcid":"https://orcid.org/0000-0001-7811-2131","observed_orcids":["https://orcid.org/0000-0001-7811-2131"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tobias S. Ulmer","raw_affiliation_strings":["Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892","Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]},{"raw_affiliation_string":"Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072359822","display_name":"Benjamin E. Ramirez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin E. Ramirez","raw_affiliation_strings":["Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061019231","display_name":"Frank Delaglio","orcid":"https://orcid.org/0000-0003-1264-2556","observed_orcids":["https://orcid.org/0000-0003-1264-2556"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Frank Delaglio","raw_affiliation_strings":["Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082289559","display_name":"Ad Bax","orcid":"https://orcid.org/0000-0002-9809-5700","observed_orcids":["https://orcid.org/0000-0002-9809-5700"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ad Bax","raw_affiliation_strings":["Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":7.615,"has_fulltext":false,"cited_by_count":305,"citation_normalized_percentile":{"value":0.9858249,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"125","issue":"30","first_page":"9179","last_page":"9191"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.9301999807357788,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.9301999807357788,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.06069999933242798,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12748","display_name":"Molecular spectroscopy and chirality","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-g","display_name":"protein G","score":0.593999981880188},{"id":"https://openalex.org/keywords/igg-binding-domain","display_name":"IgG-binding domain","score":0.824999988079071},{"id":"https://openalex.org/keywords/protein-backbone-dynamics","display_name":"backbone dynamics","score":0.5630000233650208},{"id":"https://openalex.org/keywords/protein-structure-refinement","display_name":"protein structure refinement","score":0.49900001287460327}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8842973113059998},{"id":"https://openalex.org/C89025888","wikidata":"https://www.wikidata.org/wiki/Q911907","display_name":"Dihedral angle","level":4,"score":0.7191274762153625},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.6543198823928833},{"id":"https://openalex.org/C173523689","wikidata":"https://www.wikidata.org/wiki/Q215589","display_name":"Dipole","level":2,"score":0.5827739834785461},{"id":"https://openalex.org/C193547582","wikidata":"https://www.wikidata.org/wiki/Q911331","display_name":"Molecular geometry","level":3,"score":0.565083384513855},{"id":"https://openalex.org/C150769733","wikidata":"https://www.wikidata.org/wiki/Q7315511","display_name":"Residual dipolar coupling","level":3,"score":0.5502018928527832},{"id":"https://openalex.org/C153064111","wikidata":"https://www.wikidata.org/wiki/Q1281033","display_name":"Protein crystallization","level":3,"score":0.5229461789131165},{"id":"https://openalex.org/C66974803","wikidata":"https://www.wikidata.org/wiki/Q10359898","display_name":"Nuclear magnetic resonance spectroscopy","level":2,"score":0.5143707990646362},{"id":"https://openalex.org/C31684184","wikidata":"https://www.wikidata.org/wiki/Q33293","display_name":"Peptide bond","level":3,"score":0.4562574028968811},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4554119408130646},{"id":"https://openalex.org/C155860418","wikidata":"https://www.wikidata.org/wiki/Q863695","display_name":"Bond length","level":3,"score":0.4232061207294464},{"id":"https://openalex.org/C13274807","wikidata":"https://www.wikidata.org/wiki/Q203989","display_name":"Liquid crystal","level":2,"score":0.41907694935798645},{"id":"https://openalex.org/C115624301","wikidata":"https://www.wikidata.org/wiki/Q895901","display_name":"Crystal structure","level":2,"score":0.38540834188461304},{"id":"https://openalex.org/C112887158","wikidata":"https://www.wikidata.org/wiki/Q169324","display_name":"Hydrogen bond","level":3,"score":0.2747441828250885},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.21563774347305298},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.20534241199493408},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.08119919896125793},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.08019649982452393},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.07572314143180847},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07479137182235718},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja0350684","doi":"https://doi.org/10.1021/ja0350684","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja0350684","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:15369375","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15369375","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W26207024","https://openalex.org/W162095823","https://openalex.org/W246748450","https://openalex.org/W1524149111","https://openalex.org/W1605952717","https://openalex.org/W1935237622","https://openalex.org/W1969227831","https://openalex.org/W1970419869","https://openalex.org/W1972065470","https://openalex.org/W1974519019","https://openalex.org/W1980139576","https://openalex.org/W1994876831","https://openalex.org/W1996449401","https://openalex.org/W1996700062","https://openalex.org/W2003625632","https://openalex.org/W2010795086","https://openalex.org/W2015373270","https://openalex.org/W2019738127","https://openalex.org/W2025717854","https://openalex.org/W2027238103","https://openalex.org/W2031188501","https://openalex.org/W2035479508","https://openalex.org/W2038178130","https://openalex.org/W2042513379","https://openalex.org/W2051760313","https://openalex.org/W2053590485","https://openalex.org/W2073486807","https://openalex.org/W2080837519","https://openalex.org/W2081102369","https://openalex.org/W2090189932","https://openalex.org/W2090923887","https://openalex.org/W2091562436","https://openalex.org/W2093968495","https://openalex.org/W2099356919","https://openalex.org/W2112103709","https://openalex.org/W2116551789","https://openalex.org/W2131537405","https://openalex.org/W2135212862","https://openalex.org/W2144634347","https://openalex.org/W2144731691","https://openalex.org/W2169821755","https://openalex.org/W2170756255","https://openalex.org/W2314225431","https://openalex.org/W2949250147","https://openalex.org/W4243546559"],"related_works":["https://openalex.org/W2030764004","https://openalex.org/W1991053931","https://openalex.org/W7097932631","https://openalex.org/W2146058008","https://openalex.org/W2036289338","https://openalex.org/W2103639723","https://openalex.org/W2398841253","https://openalex.org/W2061827156","https://openalex.org/W2059646694","https://openalex.org/W2003625632"],"abstract_inverted_index":{"NMR":[0],"measurements":[1],"of":[2,6,20,25,37,73,100,113,136,146,223,240,249,264,283],"a":[3,35,107,197],"large":[4],"set":[5],"protein":[7,60],"backbone":[8],"one-bond":[9],"dipolar":[10,53,83,97,210],"couplings":[11,54,84],"have":[12],"been":[13],"carried":[14],"out":[15],"to":[16,151,165,205,228],"refine":[17],"the":[18,21,41,59,74,79,86,101,111,114,124,127,131,147,166,173,185,191,194,201,215,224,229,238,244,265,268,284,290,302,310],"structure":[19,77],"third":[22],"IgG-binding":[23],"domain":[24],"Protein":[26],"G":[27],"(GB3),":[28],"previously":[29],"solved":[30],"by":[31,252,278,296],"X-ray":[32],"crystallography":[33],"at":[34,253],"resolution":[36],"1.1":[38],"A.":[39],"Besides":[40],"commonly":[42,179,216],"used":[43],"bicelle,":[44],"poly(ethylene":[45],"glycol),":[46],"and":[47,81,90,123,130,276,312],"filamentous":[48],"phage":[49],"liquid":[50],"crystalline":[51],"media,":[52],"were":[55],"also":[56],"measured":[57],"when":[58],"was":[61],"aligned":[62],"inside":[63],"either":[64],"positively":[65],"or":[66],"negatively":[67],"charged":[68],"stretched":[69],"acrylamide":[70],"gels.":[71],"Refinement":[72],"GB3":[75,266],"crystal":[76,182],"against":[78],"(13)C(alpha)-(13)C'":[80],"(13)C'-(15)N":[82],"improves":[85],"agreement":[87],"between":[88,110,126],"experimental":[89],"predicted":[91],"(15)N-(1)H(N)":[92,209],"as":[93,95,169],"well":[94],"(13)C(alpha)-(1)H(alpha)":[96],"couplings.":[98],"Evaluation":[99,282],"peptide":[102,115,148,155,230],"bond":[103,116,156,226],"N-H":[104,128,186,225],"orientations":[105],"shows":[106],"weak":[108],"anticorrelation":[109],"deviation":[112],"torsion":[117,203],"angle":[118,125,204],"omega":[119],"from":[120,154,301],"180":[121],"degrees":[122,163,300],"vector":[129,187,293],"C'-N-C(alpha)":[132,195],"plane.":[133],"The":[134,208],"slope":[135],"this":[137],"correlation":[138],"is":[139],"-1,":[140],"indicating":[141],"that,":[142],"on":[143,190],"average,":[144],"pyramidalization":[145],"N":[149],"contributes":[150],"small":[152],"deviations":[153],"planarity":[157],"(<omega>":[158],"=":[159],"179.3":[160],"+/-":[161],"3.1":[162],")":[164],"same":[167],"degree":[168],"true":[170],"twisting":[171],"around":[172],"C'-N":[174],"bond.":[175],"Although":[176],"hydrogens":[177],"are":[178,232,273],"built":[180],"onto":[181],"structures":[183],"assuming":[184],"orientation":[188,294],"falls":[189],"line":[192],"bisecting":[193],"angle,":[196],"better":[198],"approximation":[199],"adjusts":[200],"C(alpha)-C'-N-H":[202],"-2":[206],"degrees.":[207,256],"data":[211],"do":[212],"not":[213],"contradict":[214],"accepted":[217],"motional":[218],"model":[219],"where":[220],"angular":[221,241],"fluctuations":[222,242],"orthogonal":[227,243],"plane":[231,246],"larger":[233],"than":[234,280,298],"in-plane":[235,250],"motions,":[236],"but":[237],"amplitude":[239],"C(alpha)(i-1)-N(i)-C(alpha)(i)":[245],"exceeds":[247],"that":[248,261,289,304],"motions":[251],"most":[254,263],"10-15":[255],"Dipolar":[257],"coupling":[258],"analysis":[259],"indicates":[260,288],"for":[262],"backbone,":[267],"amide":[269],"order":[270],"parameters,":[271],"S,":[272],"highly":[274],"homogeneous":[275],"vary":[277],"less":[279,297],"+/-7%.":[281],"H(alpha)":[285],"proton":[286],"positions":[287],"average":[291],"C(alpha)-H(alpha)":[292],"deviates":[295],"1":[299],"direction":[303],"makes":[305],"ideal":[306],"tetrahedral":[307],"angles":[308],"with":[309],"C(alpha)-C(beta)":[311],"C(alpha)-N":[313],"vectors.":[314]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":12},{"year":2017,"cited_by_count":10},{"year":2016,"cited_by_count":16},{"year":2015,"cited_by_count":23},{"year":2014,"cited_by_count":17},{"year":2013,"cited_by_count":25},{"year":2012,"cited_by_count":17}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}