{"id":"https://openalex.org/W2069827359","doi":"https://doi.org/10.1101/pdb.prot4595","title":"Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice","display_name":"Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice","publication_year":2006,"publication_date":"2006-11-01","ids":{"openalex":"https://openalex.org/W2069827359","doi":"https://doi.org/10.1101/pdb.prot4595","mag":"2069827359","pmid":"https://pubmed.ncbi.nlm.nih.gov/22485982"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot4595","doi":"https://doi.org/10.1101/pdb.prot4595","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot4595","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018880989","display_name":"Ramasamy Paulmurugan","orcid":"https://orcid.org/0000-0001-7155-4738","observed_orcids":["https://orcid.org/0000-0001-7155-4738"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ramasam Paulmurugan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087782348","display_name":"Pritha Ray","orcid":"https://orcid.org/0000-0002-9281-2326","observed_orcids":["https://orcid.org/0000-0002-9281-2326"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Pritha Ray","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058750227","display_name":"Abhijit De","orcid":"https://orcid.org/0000-0002-5818-0206","observed_orcids":["https://orcid.org/0000-0002-5818-0206"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Abhijit De","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048102295","display_name":"Carmel T. Chan","orcid":"https://orcid.org/0000-0001-9553-0079","observed_orcids":["https://orcid.org/0000-0001-9553-0079"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Carmel T. Chan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5019955301","display_name":"Sanjiv Sam Gambhir","orcid":"https://orcid.org/0000-0002-2711-7554","observed_orcids":["https://orcid.org/0000-0002-2711-7554"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sanjir Sam Gambhir","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2006","issue":"6","first_page":"pdb.prot4595","last_page":"pdb.prot4595"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.9599999785423279},{"id":"https://openalex.org/keywords/in-vivo-imaging","display_name":"in vivo imaging","score":0.7519999742507935},{"id":"https://openalex.org/keywords/tumor-models","display_name":"tumor models","score":0.48500001430511475},{"id":"https://openalex.org/keywords/transient-transfection","display_name":"transient transfection","score":0.8259999752044678},{"id":"https://openalex.org/keywords/optical-imaging","display_name":"optical imaging","score":0.6169999837875366},{"id":"https://openalex.org/keywords/genetically-modified-animals","display_name":"transgenic animals","score":0.5830000042915344},{"id":"https://openalex.org/keywords/non-invasive-imaging","display_name":"non-invasive imaging","score":0.5509999990463257}],"concepts":[{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.7907675504684448},{"id":"https://openalex.org/C188082640","wikidata":"https://www.wikidata.org/wiki/Q1780899","display_name":"Complementation","level":4,"score":0.735844075679779},{"id":"https://openalex.org/C2225953","wikidata":"https://www.wikidata.org/wiki/Q1661899","display_name":"Inducer","level":3,"score":0.6777502298355103},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6636682748794556},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.6169166564941406},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6146061420440674},{"id":"https://openalex.org/C115867849","wikidata":"https://www.wikidata.org/wiki/Q753175","display_name":"Protein-fragment complementation assay","level":5,"score":0.5432819128036499},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5380093455314636},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4846135079860687},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4696834683418274},{"id":"https://openalex.org/C2778117688","wikidata":"https://www.wikidata.org/wiki/Q264024","display_name":"Animal model","level":2,"score":0.4497263431549072},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43547242879867554},{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.4182283580303192},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4102116525173187},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39575523138046265},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36761674284935},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3430667817592621},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.30037349462509155},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.23653051257133484},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.19786688685417175},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17599675059318542},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16687673330307007},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot4595","doi":"https://doi.org/10.1101/pdb.prot4595","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot4595","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:22485982","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22485982","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"CSH protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.504800021648407,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W1620954807"],"related_works":["https://openalex.org/W2114073111","https://openalex.org/W57616822","https://openalex.org/W264875890","https://openalex.org/W2437129925","https://openalex.org/W2091916973","https://openalex.org/W7099456682","https://openalex.org/W1620954807","https://openalex.org/W2143997071","https://openalex.org/W2052079282","https://openalex.org/W4407688678"],"abstract_inverted_index":{"This":[0],"protocol":[1],"describes":[2],"a":[3,101,111],"split":[4],"luciferase":[5],"complementation":[6],"assay":[7],"that":[8],"can":[9,41,82,91,130],"be":[10,42,83,107,131,158,182],"used":[11],"to":[12,109,160,163],"repetitively":[13],"and":[14,89],"noninvasively":[15],"study":[16],"the":[17,26,29,70,77,95,116,122,142,150,155,172],"interaction":[18],"of":[19,28,54,79,166,174],"proteins":[20],"in":[21,35,38,136,140],"small":[22],"living":[23],"animals.":[24],"After":[25],"expression":[27],"appropriate":[30],"vectors":[31],"has":[32],"been":[33,146],"checked":[34],"cell":[36],"culture":[37],"vivo,":[39],"studies":[40],"performed":[43],"either":[44],"by":[45],"implanting":[46],"transiently":[47],"transfected":[48],"cells":[49,67,80],"for":[50],"short-term":[51],"analysis":[52],"(maximum":[53],"7":[55],"days),":[56],"as":[57,179],"described":[58],"below,":[59],"or":[60,118],"with":[61,115],"tumor":[62,66],"models":[63,126],"grown":[64],"from":[65],"stably":[68,147],"expressing":[69],"complete":[71],"reporter":[72,103,143],"system.":[73],"For":[74,168],"optical":[75],"imaging,":[76],"number":[78],"implanted":[81],"relatively":[84],"low":[85],"(~1-5":[86],"\u00d7":[87],"10(6)),":[88],"imaging":[90,176],"begin":[92],"even":[93],"before":[94,120],"tumors":[96],"are":[97],"palpable.":[98],"When":[99],"using":[100],"regulated":[102],"gene,":[104],"it":[105],"may":[106],"necessary":[108],"perform":[110],"dose-dependent":[112],"pilot":[113],"experiment":[114],"inducer":[117],"repressor":[119],"performing":[121],"primary":[123],"experiments.":[124],"Animal":[125],"other":[127,175],"than":[128,154],"mice":[129],"used,":[132],"including":[133],"rats":[134],"and,":[135],"theory,":[137],"transgenic":[138],"animals":[139],"which":[141],"constructs":[144],"have":[145],"integrated":[148],"into":[149],"genome.":[151],"Animals":[152],"larger":[153],"rat":[156],"would":[157],"difficult":[159],"image":[161],"due":[162],"poor":[164],"penetration":[165],"light.":[167],"these":[169],"larger-animal":[170],"models,":[171],"use":[173],"technologies":[177],"such":[178],"microPET":[180],"should":[181],"considered.":[183]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}
