{"id":"https://openalex.org/W2069746331","doi":"https://doi.org/10.1002/jms.1190301012","title":"Matrix\u2010assisted laser desorption/ionization mass spectrometry (MALDI\u2010MS) of membrane proteins and non\u2010covalent complexes","display_name":"Matrix\u2010assisted laser desorption/ionization mass spectrometry (MALDI\u2010MS) of membrane proteins and non\u2010covalent complexes","publication_year":1995,"publication_date":"1995-10-01","ids":{"openalex":"https://openalex.org/W2069746331","doi":"https://doi.org/10.1002/jms.1190301012","mag":"2069746331"},"language":"en","primary_location":{"id":"doi:10.1002/jms.1190301012","doi":"https://doi.org/10.1002/jms.1190301012","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jms.1190301012","pdf_url":null,"source":{"id":"https://openalex.org/S37349105","display_name":"Journal of Mass Spectrometry","issn_l":"1076-5174","issn":["1076-5174","1096-9888"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Mass Spectrometry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2069746331"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046820992","display_name":"Burkhard Rosinke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22465464","display_name":"University of M\u00fcnster","ror":"https://ror.org/00pd74e08","country_code":"DE","type":"education","lineage":["https://openalex.org/I22465464"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Burkhard Rosinke","raw_affiliation_strings":["Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","institution_ids":["https://openalex.org/I22465464"]},{"raw_affiliation_string":"Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany","institution_ids":["https://openalex.org/I22465464"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026349053","display_name":"Kerstin Strupat","orcid":"https://orcid.org/0009-0007-2001-8768","observed_orcids":["https://orcid.org/0009-0007-2001-8768"]},"institutions":[{"id":"https://openalex.org/I22465464","display_name":"University of M\u00fcnster","ror":"https://ror.org/00pd74e08","country_code":"DE","type":"education","lineage":["https://openalex.org/I22465464"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Kerstin Strupat","raw_affiliation_strings":["Institute for Medical Physics and Biophysics, University of Mu\u0308nster, Robert-Koch-Str. 31, D-48149 Mu\u0308nster, Germany","Institute for Medical Physics and Biophysics, University of M\u00fcnster, Robert-Koch-Str. 31, D-48149 M\u00fcnster, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Medical Physics and Biophysics, University of Mu\u0308nster, Robert-Koch-Str. 31, D-48149 Mu\u0308nster, Germany","institution_ids":["https://openalex.org/I22465464"]},{"raw_affiliation_string":"Institute for Medical Physics and Biophysics, University of M\u00fcnster, Robert-Koch-Str. 31, D-48149 M\u00fcnster, Germany","institution_ids":["https://openalex.org/I22465464"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009458005","display_name":"Franz Hillenkamp","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22465464","display_name":"University of M\u00fcnster","ror":"https://ror.org/00pd74e08","country_code":"DE","type":"education","lineage":["https://openalex.org/I22465464"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Franz Hillenkamp","raw_affiliation_strings":["Institute for Medical Physics and Biophysics, University of Mu\u0308nster, Robert-Koch-Str. 31, D-48149 Mu\u0308nster, Germany","Institute for Medical Physics and Biophysics, University of M\u00fcnster, Robert-Koch-Str. 31, D-48149 M\u00fcnster, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Medical Physics and Biophysics, University of Mu\u0308nster, Robert-Koch-Str. 31, D-48149 Mu\u0308nster, Germany","institution_ids":["https://openalex.org/I22465464"]},{"raw_affiliation_string":"Institute for Medical Physics and Biophysics, University of M\u00fcnster, Robert-Koch-Str. 31, D-48149 M\u00fcnster, Germany","institution_ids":["https://openalex.org/I22465464"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018312697","display_name":"J\u00fcrg P. Rosenbusch","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J\u00fcrg Rosenbusch","raw_affiliation_strings":["Maurice-Mu\u0308ller Institute, Biozentrum Basel, Klingelbergstr. 70, CH4057 Basel, Switzerland","Maurice-M\u00fcller Institute, Biozentrum Basel, Klingelbergstr. 70, CH4057 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Maurice-Mu\u0308ller Institute, Biozentrum Basel, Klingelbergstr. 70, CH4057 Basel, Switzerland","institution_ids":[]},{"raw_affiliation_string":"Maurice-M\u00fcller Institute, Biozentrum Basel, Klingelbergstr. 70, CH4057 Basel, Switzerland","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082961606","display_name":"Norbert A. Dencher","orcid":"https://orcid.org/0000-0002-1745-8670","observed_orcids":["https://orcid.org/0000-0002-1745-8670"]},"institutions":[{"id":"https://openalex.org/I31512782","display_name":"Technische Universit\u00e4t Darmstadt","ror":"https://ror.org/05n911h24","country_code":"DE","type":"education","lineage":["https://openalex.org/I31512782"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Norbert Dencher","raw_affiliation_strings":["Institute of Biochemistry, Technical University of Darmstadt, Petersenstr. 22, D-64287 Darmstadt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry, Technical University of Darmstadt, Petersenstr. 22, D-64287 Darmstadt, Germany","institution_ids":["https://openalex.org/I31512782"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ulrike Kr\u00fcger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22465464","display_name":"University of M\u00fcnster","ror":"https://ror.org/00pd74e08","country_code":"DE","type":"education","lineage":["https://openalex.org/I22465464"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ulrike Kr\u00fcger","raw_affiliation_strings":["Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","institution_ids":["https://openalex.org/I22465464"]},{"raw_affiliation_string":"Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany","institution_ids":["https://openalex.org/I22465464"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000919967","display_name":"Hans\u2010Joachim Galla","orcid":"https://orcid.org/0000-0003-1018-052X","observed_orcids":["https://orcid.org/0000-0003-1018-052X"]},"institutions":[{"id":"https://openalex.org/I22465464","display_name":"University of M\u00fcnster","ror":"https://ror.org/00pd74e08","country_code":"DE","type":"education","lineage":["https://openalex.org/I22465464"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hans\u2010Joachim Galla","raw_affiliation_strings":["Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry, University of Mu\u0308nster, Wilhelm-Klemm-Str. 2, D-48149 Mu\u0308nster, Germany","institution_ids":["https://openalex.org/I22465464"]},{"raw_affiliation_string":"Institute of Biochemistry, University of M\u00fcnster, Wilhelm-Klemm-Str. 2, D-48149 M\u00fcnster, Germany","institution_ids":["https://openalex.org/I22465464"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":7.3136,"has_fulltext":false,"cited_by_count":185,"citation_normalized_percentile":{"value":0.98518049,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"30","issue":"10","first_page":"1462","last_page":"1468"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/maldi-mass-spectrometry","display_name":"MALDI mass spectrometry","score":0.8640000224113464},{"id":"https://openalex.org/keywords/membrane-proteins","display_name":"membrane proteins","score":0.9940000176429749},{"id":"https://openalex.org/keywords/noncovalent-protein-complexes","display_name":"noncovalent protein complexes","score":0.8999999761581421},{"id":"https://openalex.org/keywords/porin","display_name":"porin","score":0.925000011920929},{"id":"https://openalex.org/keywords/bacteriorhodopsin","display_name":"bacteriorhodopsin","score":0.9120000004768372},{"id":"https://openalex.org/keywords/detergents","display_name":"detergents","score":0.8870000243186951},{"id":"https://openalex.org/keywords/protein-sample-preparation","display_name":"protein sample preparation","score":0.5979999899864197}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.923952579498291},{"id":"https://openalex.org/C2777317804","wikidata":"https://www.wikidata.org/wiki/Q67101192","display_name":"Porin","level":5,"score":0.806698739528656},{"id":"https://openalex.org/C189542057","wikidata":"https://www.wikidata.org/wiki/Q242310","display_name":"Bacteriorhodopsin","level":3,"score":0.7822397351264954},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.7620725035667419},{"id":"https://openalex.org/C75280812","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Matrix-assisted laser desorption/ionization","level":4,"score":0.7193089127540588},{"id":"https://openalex.org/C162711632","wikidata":"https://www.wikidata.org/wiki/Q905514","display_name":"Desorption","level":3,"score":0.6419472694396973},{"id":"https://openalex.org/C40325409","wikidata":"https://www.wikidata.org/wiki/Q2360668","display_name":"Mass spectrum","level":3,"score":0.5099447965621948},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4589690864086151},{"id":"https://openalex.org/C2778113554","wikidata":"https://www.wikidata.org/wiki/Q3782756","display_name":"Halobacterium salinarum","level":4,"score":0.4543735682964325},{"id":"https://openalex.org/C67839113","wikidata":"https://www.wikidata.org/wiki/Q6787840","display_name":"Matrix-assisted laser desorption electrospray ionization","level":5,"score":0.4389874041080475},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.41374915838241577},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.39869219064712524},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.38891083002090454},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.33487826585769653},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.28261733055114746},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12817507982254028},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1116517186164856},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/jms.1190301012","doi":"https://doi.org/10.1002/jms.1190301012","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jms.1190301012","pdf_url":null,"source":{"id":"https://openalex.org/S37349105","display_name":"Journal of Mass Spectrometry","issn_l":"1076-5174","issn":["1076-5174","1096-9888"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Mass Spectrometry","raw_type":"journal-article"},{"id":"pmh:oai:tubiblio.ulb.tu-darmstadt.de:2519","doi":null,"is_oa":false,"landing_page_url":"http://tubiblio.ulb.tu-darmstadt.de/2519/","pdf_url":null,"source":{"id":"https://openalex.org/S4377196390","display_name":"TUbilio (Technical University of Darmstadt)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I31512782","host_organization_name":"Technische Universit\u00e4t Darmstadt","host_organization_lineage":["https://openalex.org/I31512782"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Artikel"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1530423872","https://openalex.org/W1576350060","https://openalex.org/W1972934137","https://openalex.org/W1975509906","https://openalex.org/W1981210320","https://openalex.org/W1988287443","https://openalex.org/W1988928440","https://openalex.org/W2014323182","https://openalex.org/W2035817126","https://openalex.org/W2053246483","https://openalex.org/W2072671787","https://openalex.org/W2123672994","https://openalex.org/W2743792571","https://openalex.org/W4251700885"],"related_works":["https://openalex.org/W2059377636","https://openalex.org/W2768163076","https://openalex.org/W1988928440","https://openalex.org/W1987781203","https://openalex.org/W1976788847","https://openalex.org/W2037186777","https://openalex.org/W1980071838","https://openalex.org/W140870587","https://openalex.org/W1996929559","https://openalex.org/W4384200675"],"abstract_inverted_index":{"Abstract":[0],"Matrix\u2010assisted":[1],"laser":[2],"desorption/ionization":[3],"(MALDI)":[4],"mass":[5,49,65],"spectra":[6],"and":[7,28,37,54],"methods":[8],"to":[9,51,59,98],"improve":[10],"their":[11],"quality":[12,62],"are":[13],"reported":[14],"for":[15],"three":[16,75],"hydrophobic,":[17],"membrane\u2010bound":[18],"proteins:":[19],"porin":[20],"from":[21,25,30],"Escherichia":[22],"coli,":[23],"bacteriorhodopsin":[24],"Halobacterium":[26],"salinarium":[27],"cholesterolesterase":[29],"Pseudomonas":[31],"fluorescens.":[32],"Several":[33],"commonly":[34],"used":[35],"UV":[36],"IR":[38],"matrices":[39],"have":[40],"been":[41,68],"tested.":[42],"In":[43],"addition,":[44],"the":[45,61,88,91,103,111],"susceptibility":[46],"of":[47,63,74,90,102],"MALDI":[48,64],"spectrometry":[50],"various":[52],"neutral":[53],"ionic":[55],"detergents,":[56],"known":[57],"usually":[58],"degrade":[60],"spectra,":[66],"has":[67],"tested":[69],"systematically.":[70],"For":[71],"porin,":[72],"consisting":[73],"identical":[76],"non\u2010covalently":[77],"bound":[78],"subunits,":[79],"a":[80,99,105],"new":[81],"sample":[82],"preparation":[83],"is":[84,108],"reported,":[85],"resulting":[86],"in":[87],"desorption":[89],"intact":[92],"quaternary":[93],"protein":[94],"structure.":[95],"This":[96],"leads":[97],"better":[100],"understanding":[101],"way":[104],"given":[106],"analyte":[107],"embedded":[109],"into":[110],"host":[112],"matrix":[113],"crystals.":[114]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}