{"id":"https://openalex.org/W2068939636","doi":"https://doi.org/10.1271/bbb.60623","title":"Highly Induced DNA Recombination Mediated by Membrane Permeabilized Recombinant Cre Protein in Mouse Primary Cells","display_name":"Highly Induced DNA Recombination Mediated by Membrane Permeabilized Recombinant Cre Protein in Mouse Primary Cells","publication_year":2007,"publication_date":"2007-03-23","ids":{"openalex":"https://openalex.org/W2068939636","doi":"https://doi.org/10.1271/bbb.60623","mag":"2068939636","pmid":"https://pubmed.ncbi.nlm.nih.gov/17341820"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.60623","doi":"https://doi.org/10.1271/bbb.60623","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60623","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5073114368","display_name":"Shizu Hidema","orcid":"https://orcid.org/0000-0003-0554-0623","observed_orcids":["https://orcid.org/0000-0003-0554-0623"]},"institutions":[{"id":"https://openalex.org/I201537933","display_name":"Tohoku University","ror":"https://ror.org/01dq60k83","country_code":"JP","type":"education","lineage":["https://openalex.org/I201537933"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shizu HIDEMA","raw_affiliation_strings":["Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan","Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan","institution_ids":["https://openalex.org/I201537933"]},{"raw_affiliation_string":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University","institution_ids":["https://openalex.org/I201537933"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029506679","display_name":"Takamitsu Maruyama","orcid":"https://orcid.org/0000-0001-7322-8821","observed_orcids":["https://orcid.org/0000-0001-7322-8821"]},"institutions":[{"id":"https://openalex.org/I201537933","display_name":"Tohoku University","ror":"https://ror.org/01dq60k83","country_code":"JP","type":"education","lineage":["https://openalex.org/I201537933"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takamitsu MARUYAMA","raw_affiliation_strings":["Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University","institution_ids":["https://openalex.org/I201537933"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043143316","display_name":"Shigek\u00ed Kato","orcid":"https://orcid.org/0000-0002-5768-7240","observed_orcids":["https://orcid.org/0000-0002-5768-7240"]},"institutions":[{"id":"https://openalex.org/I201537933","display_name":"Tohoku University","ror":"https://ror.org/01dq60k83","country_code":"JP","type":"education","lineage":["https://openalex.org/I201537933"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigeki KATO","raw_affiliation_strings":["Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University","institution_ids":["https://openalex.org/I201537933"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084957793","display_name":"Katsuhiko Nishimori","orcid":"https://orcid.org/0000-0001-9921-2211","observed_orcids":["https://orcid.org/0000-0001-9921-2211"]},"institutions":[{"id":"https://openalex.org/I201537933","display_name":"Tohoku University","ror":"https://ror.org/01dq60k83","country_code":"JP","type":"education","lineage":["https://openalex.org/I201537933"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Katsuhiko NISHIMORI","raw_affiliation_strings":["Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University","institution_ids":["https://openalex.org/I201537933"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201537933"],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.13353853,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"71","issue":"3","first_page":"817","last_page":"820"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0575999990105629,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.006099999882280827,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cre-recombinase","display_name":"Cre recombinase","score":0.9259999990463257},{"id":"https://openalex.org/keywords/cell-penetrating-peptides","display_name":"cell-penetrating peptides","score":0.8309999704360962},{"id":"https://openalex.org/keywords/membrane-permeabilization","display_name":"membrane permeabilization","score":0.8820000290870667},{"id":"https://openalex.org/keywords/primary-batteries","display_name":"primary cells","score":0.652999997138977},{"id":"https://openalex.org/keywords/mouse-embryonic-fibroblasts","display_name":"mouse embryonic fibroblasts","score":0.9739999771118164},{"id":"https://openalex.org/keywords/keratinocytes","display_name":"keratinocytes","score":0.9940000176429749},{"id":"https://openalex.org/keywords/preosteoblasts","display_name":"preosteoblasts","score":0.9639999866485596}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.789095401763916},{"id":"https://openalex.org/C1292079","wikidata":"https://www.wikidata.org/wiki/Q3817769","display_name":"Transactivation","level":4,"score":0.739033043384552},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.7294983267784119},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5887459516525269},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5698374509811401},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5428956747055054},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.5331266522407532},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.477333128452301},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.47242581844329834},{"id":"https://openalex.org/C147905033","wikidata":"https://www.wikidata.org/wiki/Q1378887","display_name":"Primary cell","level":3,"score":0.45383524894714355},{"id":"https://openalex.org/C2780381497","wikidata":"https://www.wikidata.org/wiki/Q463418","display_name":"Fibroblast","level":3,"score":0.41278257966041565},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3357123136520386},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33492201566696167},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3223528563976288},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.26110032200813293},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2544161379337311},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22527912259101868},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10323125123977661}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002463","descriptor_name":"Cell Membrane Permeability","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015603","descriptor_name":"Keratinocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.60623","doi":"https://doi.org/10.1271/bbb.60623","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60623","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:17341820","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17341820","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.7246000170707703,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1975627280","https://openalex.org/W2000286590","https://openalex.org/W2011293012","https://openalex.org/W2042796276","https://openalex.org/W2062769438","https://openalex.org/W2172939187","https://openalex.org/W4249775659"],"related_works":["https://openalex.org/W2141803553","https://openalex.org/W7146153424","https://openalex.org/W2289349660","https://openalex.org/W1975627280","https://openalex.org/W2886964576","https://openalex.org/W1547305417","https://openalex.org/W2001921044","https://openalex.org/W2000286590","https://openalex.org/W2607547170","https://openalex.org/W7079502982"],"abstract_inverted_index":{"To":[0],"establish":[1],"efficient":[2],"induction":[3],"of":[4,26,36,56],"Cre":[5,46],"mediated":[6],"DNA":[7,57],"recombination":[8,58],"in":[9,30],"primary":[10,17,61],"cells,":[11],"mouse":[12],"embryonic":[13],"fibroblast,":[14],"keratinocyte,":[15],"and":[16],"preosteoblast,":[18],"we":[19],"tested":[20],"various":[21],"recombinant":[22],"Cres":[23],"by":[24],"fusing":[25],"protein":[27],"transduction":[28],"domain":[29],"human":[31],"immunodeficiency":[32],"virus":[33],"(HIV)":[34],"transactivator":[35],"transcription":[37],"(TAT-PTD)":[38],"to":[39],"the":[40,50,53],"N-":[41],"and/or":[42],"C-terminus.":[43],"HTC,":[44],"modified":[45],"with":[47],"PTD":[48],"at":[49],"N-terminus,":[51],"achieved":[52],"highest":[54],"activity":[55],"for":[59],"those":[60],"cells.":[62]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}