{"id":"https://openalex.org/W2068912874","doi":"https://doi.org/10.1021/jf0581269","title":"Biosynthesis of 14C-Phytoene from Tomato Cell Suspension Cultures (Lycopersicon esculentum) for Utilization in Prostate Cancer Cell Culture Studies","display_name":"Biosynthesis of 14C-Phytoene from Tomato Cell Suspension Cultures (Lycopersicon esculentum) for Utilization in Prostate Cancer Cell Culture Studies","publication_year":2006,"publication_date":"2006-01-11","ids":{"openalex":"https://openalex.org/W2068912874","doi":"https://doi.org/10.1021/jf0581269","mag":"2068912874","pmid":"https://pubmed.ncbi.nlm.nih.gov/16448178"},"language":"en","primary_location":{"id":"doi:10.1021/jf0581269","doi":"https://doi.org/10.1021/jf0581269","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jf0581269","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112059605","display_name":"Jessica K. Campbell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica K. Campbell","raw_affiliation_strings":["Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005381024","display_name":"Randy B. Rogers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Randy B. Rogers","raw_affiliation_strings":["Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064334824","display_name":"Mary Ann Lila","orcid":"https://orcid.org/0000-0002-4928-3836","observed_orcids":["https://orcid.org/0000-0002-4928-3836"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mary Ann Lila","raw_affiliation_strings":["Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033908566","display_name":"John W. Erdman","orcid":"https://orcid.org/0000-0001-7924-6596","observed_orcids":["https://orcid.org/0000-0001-7924-6596"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John W. Erdman","raw_affiliation_strings":["Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Nutritional Sciences, University of Illinois at UrbanaChampaign, 905 South Goodwin Avenue, and Department of Natural Resources and Environmental Sciences, University of Illinois at UrbanaChampaign, 1201 South Dorner Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5033908566"],"corresponding_institution_ids":["https://openalex.org/I157725225"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.7857,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.63451028,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"54","issue":"3","first_page":"747","last_page":"755"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.6259999871253967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.6259999871253967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.36039999127388,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10445","display_name":"Antioxidant Activity and Oxidative Stress","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2704","display_name":"Biochemistry (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phytofluene","display_name":"phytofluene","score":0.9750000238418579},{"id":"https://openalex.org/keywords/tomato","display_name":"tomato","score":0.8999999761581421},{"id":"https://openalex.org/keywords/norflurazon","display_name":"norflurazon","score":0.9480000138282776},{"id":"https://openalex.org/keywords/radiolabeling","display_name":"radiolabeling","score":0.7789999842643738},{"id":"https://openalex.org/keywords/carotenoids","display_name":"carotenoids","score":0.6779999732971191},{"id":"https://openalex.org/keywords/du145-cells","display_name":"DU145 cells","score":0.9229999780654907},{"id":"https://openalex.org/keywords/chemoprevention","display_name":"chemoprevention","score":0.859000027179718}],"concepts":[{"id":"https://openalex.org/C2777680332","wikidata":"https://www.wikidata.org/wiki/Q544652","display_name":"Lycopersicon","level":2,"score":0.937753438949585},{"id":"https://openalex.org/C2776809771","wikidata":"https://www.wikidata.org/wiki/Q302247","display_name":"Phytoene","level":4,"score":0.7310463786125183},{"id":"https://openalex.org/C2993787908","wikidata":"https://www.wikidata.org/wiki/Q189111","display_name":"Suspension culture","level":3,"score":0.6710734367370605},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6337844133377075},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.5885981917381287},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4555839002132416},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.44887450337409973},{"id":"https://openalex.org/C2778761015","wikidata":"https://www.wikidata.org/wiki/Q134172","display_name":"Solanaceae","level":3,"score":0.44373035430908203},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4252725839614868},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.41658106446266174},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.41648396849632263},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.41314393281936646},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3384366035461426},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20354807376861572},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.15420737862586975},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14128509163856506}],"mesh":[{"descriptor_ui":"D000230","descriptor_name":"Adenocarcinoma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002250","descriptor_name":"Carbon Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002338","descriptor_name":"Carotenoids","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D002338","descriptor_name":"Carotenoids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011471","descriptor_name":"Prostatic Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018551","descriptor_name":"Solanum lycopersicum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/jf0581269","doi":"https://doi.org/10.1021/jf0581269","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jf0581269","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},{"id":"pmid:16448178","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16448178","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of agricultural and food chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.6875,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":50,"referenced_works":["https://openalex.org/W1556410020","https://openalex.org/W1812976842","https://openalex.org/W1823036198","https://openalex.org/W1880406288","https://openalex.org/W1905313439","https://openalex.org/W1918723691","https://openalex.org/W1921006759","https://openalex.org/W1950058861","https://openalex.org/W1965890116","https://openalex.org/W1970240465","https://openalex.org/W1982528320","https://openalex.org/W2001130734","https://openalex.org/W2001314288","https://openalex.org/W2013747970","https://openalex.org/W2016592043","https://openalex.org/W2024556021","https://openalex.org/W2033177883","https://openalex.org/W2034111313","https://openalex.org/W2034753931","https://openalex.org/W2040620829","https://openalex.org/W2048641359","https://openalex.org/W2053033255","https://openalex.org/W2057390168","https://openalex.org/W2057889431","https://openalex.org/W2067148898","https://openalex.org/W2076429988","https://openalex.org/W2076729844","https://openalex.org/W2084891397","https://openalex.org/W2089565678","https://openalex.org/W2091536118","https://openalex.org/W2092256521","https://openalex.org/W2094945194","https://openalex.org/W2102950247","https://openalex.org/W2104507397","https://openalex.org/W2125185188","https://openalex.org/W2130429874","https://openalex.org/W2130590493","https://openalex.org/W2134959352","https://openalex.org/W2139119349","https://openalex.org/W2142337757","https://openalex.org/W2143086468","https://openalex.org/W2152598135","https://openalex.org/W2155263753","https://openalex.org/W2163818055","https://openalex.org/W2164157942","https://openalex.org/W2214363225","https://openalex.org/W2265111609","https://openalex.org/W2334980278","https://openalex.org/W4235607766","https://openalex.org/W4243243349"],"related_works":["https://openalex.org/W51062924","https://openalex.org/W1975568368","https://openalex.org/W621558802","https://openalex.org/W3177387264","https://openalex.org/W2091766887","https://openalex.org/W38484978","https://openalex.org/W2290971437","https://openalex.org/W1582590535","https://openalex.org/W2041841260","https://openalex.org/W2082170436"],"abstract_inverted_index":{"This":[0],"work":[1],"describes":[2],"the":[3,46,51,119,125,161,190],"development":[4],"and":[5,16,19,48,55,63,72,74,86,94,148,157,168,185,194],"utilization":[6],"of":[7,37,50,76,91,101,116,127,152,192],"a":[8,177],"plant":[9],"cell":[10,24,34,121,134,174],"culture":[11,25,77,122],"production":[12,126],"approach":[13],"to":[14,32,44,79,83,118,165],"biosynthesize":[15],"radiolabel":[17],"phytoene":[18,54,85,93,129,153,193],"phytofluene":[20,87,95,195],"for":[21,130,181],"prostate":[22,132,140],"cancer":[23,197],"studies.":[26,136],"The":[27,163],"herbicide":[28],"norflurazon":[29,80],"was":[30,144],"added":[31],"established":[33],"suspension":[35],"cultures":[36,175],"tomato":[38,120,173],"(Lycopersicon":[39],"esculentum":[40],"cv.":[41],"VFNT":[42],"cherry),":[43],"induce":[45],"biosynthesis":[47],"accumulation":[49],"lycopene":[52],"precursors,":[53],"phytofluene,":[56],"in":[57,111,183,186,196],"their":[58],"natural":[59],"isomeric":[60],"forms":[61],"(15-cis-phytoene":[62],"two":[64],"cis-phytofluene":[65],"isomers).":[66],"Norflurazon":[67],"concentrations,":[68],"solvent":[69,113],"carrier":[70],"type":[71],"concentration,":[73],"duration":[75],"exposure":[78],"were":[81,96,154],"screened":[82],"optimize":[84],"synthesis.":[88],"Maximum":[89],"yields":[90],"both":[92],"achieved":[97],"after":[98],"7":[99],"days":[100],"treatment":[102],"with":[103],"0.03":[104],"mg":[105],"norflurazon/40":[106],"mL":[107],"fresh":[108],"medium,":[109],"provided":[110],"0.07%":[112],"carrier.":[114],"Introduction":[115],"14C-sucrose":[117],"medium":[123],"enabled":[124],"14C-labeled":[128],"subsequent":[131],"tumor":[133,141],"uptake":[135],"In":[137],"DU":[138],"145":[139],"cells,":[142],"it":[143],"determined":[145],"that":[146],"15-cis-phytoene":[147],"an":[149],"oxidized":[150],"product":[151],"taken":[155],"up":[156],"partially":[158],"metabolized":[159],"by":[160],"cells.":[162],"ability":[164],"biosynthesize,":[166],"radiolabel,":[167],"isolate":[169],"these":[170],"carotenoids":[171],"from":[172],"is":[176],"novel,":[178],"valuable":[179],"methodology":[180],"further":[182],"vitro":[184],"vivo":[187],"investigations":[188],"into":[189],"roles":[191],"chemoprevention.":[198]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}