{"id":"https://openalex.org/W2068249262","doi":"https://doi.org/10.1021/bi00017a011","title":"Catalytic characterization of 4a-hydroxytetrahydropterin dehydratase","display_name":"Catalytic characterization of 4a-hydroxytetrahydropterin dehydratase","publication_year":1995,"publication_date":"1995-05-02","ids":{"openalex":"https://openalex.org/W2068249262","doi":"https://doi.org/10.1021/bi00017a011","mag":"2068249262","pmid":"https://pubmed.ncbi.nlm.nih.gov/7727440"},"language":"en","primary_location":{"id":"doi:10.1021/bi00017a011","doi":"https://doi.org/10.1021/bi00017a011","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00017a011","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033096297","display_name":"Igor Rebrin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Igor Rebrin","raw_affiliation_strings":["Department of Pharmacology, College of Medicine, University of South Alabama, Mobile 36688, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, College of Medicine, University of South Alabama, Mobile 36688, USA","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104667904","display_name":"Steven Bailey","orcid":"https://orcid.org/0009-0005-0384-8810","observed_orcids":["https://orcid.org/0009-0005-0384-8810"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Steven W. Bailey","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035204801","display_name":"Scott R. Boerth","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Scott R. Boerth","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005825766","display_name":"Michelle D. Ardell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michelle D. Ardell","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5085725014","display_name":"June E. Ayling","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"June E. Ayling","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.2239,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.8877154,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"34","issue":"17","first_page":"5801","last_page":"5810"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11027","display_name":"Metabolism and Genetic Disorders","score":0.5791000127792358,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11027","display_name":"Metabolism and Genetic Disorders","score":0.5791000127792358,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.3034999966621399,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.023099999874830246,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aromatic-amino-acid-hydroxylases","display_name":"aromatic amino acid hydroxylases","score":0.875},{"id":"https://openalex.org/keywords/dihydropteridine-reductase","display_name":"dihydropteridine reductase","score":0.8320000171661377},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.5659999847412109},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.5690000057220459},{"id":"https://openalex.org/keywords/acid-base-catalysis","display_name":"acid-base catalysis","score":0.8650000095367432},{"id":"https://openalex.org/keywords/phenylalanine-hydroxylase","display_name":"phenylalanine hydroxylase","score":0.8999999761581421},{"id":"https://openalex.org/keywords/rat-liver-enzyme","display_name":"rat liver enzyme","score":0.5019999742507935},{"id":"https://openalex.org/keywords/cofactor-metabolism","display_name":"cofactor metabolism","score":0.49900001287460327}],"concepts":[{"id":"https://openalex.org/C2781374117","wikidata":"https://www.wikidata.org/wiki/Q5252382","display_name":"Dehydratase","level":3,"score":0.9093568325042725},{"id":"https://openalex.org/C2776145790","wikidata":"https://www.wikidata.org/wiki/Q419808","display_name":"Tetrahydrobiopterin","level":4,"score":0.8193344473838806},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7465180158615112},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.6408681273460388},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.6261721849441528},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5982853770256042},{"id":"https://openalex.org/C207009774","wikidata":"https://www.wikidata.org/wiki/Q420604","display_name":"Phenylalanine hydroxylase","level":4,"score":0.5665928721427917},{"id":"https://openalex.org/C2777992278","wikidata":"https://www.wikidata.org/wiki/Q705605","display_name":"Aromatic amino acids","level":3,"score":0.5429763793945312},{"id":"https://openalex.org/C2777431362","wikidata":"https://www.wikidata.org/wiki/Q170545","display_name":"Phenylalanine","level":3,"score":0.494260311126709},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4845646917819977},{"id":"https://openalex.org/C73969872","wikidata":"https://www.wikidata.org/wiki/Q899698","display_name":"Turnover number","level":3,"score":0.48205456137657166},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.47045814990997314},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.4167467951774597},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4065094590187073}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001708","descriptor_name":"Biopterins","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D001708","descriptor_name":"Biopterins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001708","descriptor_name":"Biopterins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010651","descriptor_name":"Phenylalanine Hydroxylase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D010651","descriptor_name":"Phenylalanine Hydroxylase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010758","descriptor_name":"Phosphorus","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011622","descriptor_name":"Pterins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00017a011","doi":"https://doi.org/10.1021/bi00017a011","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00017a011","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:7727440","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7727440","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6705407289","display_name":"TETRAHYDROPTERIN TREATMENT FOR NEUROLOGICAL DISORDERS","funder_award_id":"5r01ns026662-07","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G8380313507","display_name":null,"funder_award_id":"NS-26662","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W99674609","https://openalex.org/W1491056713","https://openalex.org/W1504005082","https://openalex.org/W1536680581","https://openalex.org/W1539302371","https://openalex.org/W1540374583","https://openalex.org/W1626591090","https://openalex.org/W1973968247","https://openalex.org/W1976085354","https://openalex.org/W1989766948","https://openalex.org/W1990956644","https://openalex.org/W1992081954","https://openalex.org/W2006305640","https://openalex.org/W2006742743","https://openalex.org/W2008716959","https://openalex.org/W2020479263","https://openalex.org/W2026447547","https://openalex.org/W2027957895","https://openalex.org/W2030447631","https://openalex.org/W2030954399","https://openalex.org/W2062944414","https://openalex.org/W2066849908","https://openalex.org/W2083644366","https://openalex.org/W2087288778","https://openalex.org/W2097865768","https://openalex.org/W2103500225","https://openalex.org/W2117481239","https://openalex.org/W2166367888","https://openalex.org/W2312565139","https://openalex.org/W2473559821","https://openalex.org/W2953001511"],"related_works":["https://openalex.org/W2021069540","https://openalex.org/W2059353739","https://openalex.org/W2394759126","https://openalex.org/W7097961533","https://openalex.org/W1964688681","https://openalex.org/W1258636314","https://openalex.org/W1598027518","https://openalex.org/W2039159275","https://openalex.org/W2166367888","https://openalex.org/W2009582277"],"abstract_inverted_index":{"The":[0,52,69,124,152],"cofactor":[1,45,215],"product":[2,178],"of":[3,46,55,79,82,131,139,180,206,213],"the":[4,25,36,43,47,75,80,132,187,211,217,222],"aromatic":[5],"amino":[6,38],"acid":[7,39,165],"hydroxylases,":[8],"4a-hydroxy-6(R)-tetrahydrobiopterin,":[9],"requires":[10],"dehydration":[11,21],"before":[12],"tetrahydrobiopterin":[13,214],"can":[14],"be":[15,176,193,226],"regenerated":[16],"by":[17,30],"dihydropteridine":[18],"reductase.":[19],"Carbinolamine":[20],"occurs":[22],"nonenzymatically,":[23],"but":[24,143],"reaction":[26],"is":[27,184,199],"also":[28],"catalyzed":[29],"4a-hydroxytetrahydropterin":[31],"dehydratase.":[32],"This":[33],"enzyme":[34,70,153],"has":[35],"identical":[37],"sequence":[40],"to":[41,74,175,192,203,209],"DCoH,":[42],"dimerization":[44],"transcription":[48],"regulator,":[49],"HNF-1":[50],"alpha.":[51],"catalytic":[53],"activity":[54],"rat":[56,197],"liver":[57],"dehydratase":[58,133,188],"was":[59,146,172,190],"characterized":[60],"using":[61],"a":[62,177],"new":[63],"assay":[64],"employing":[65],"chemically":[66],"synthesized":[67],"4a-hydroxytetrahydropterins.":[68],"shows":[71],"little":[72],"sensitivity":[73],"structure":[76],"or":[77,150],"configuration":[78],"6-substituent":[81],"its":[83],"substrate,":[84],"with":[85,134,148],"Km's":[86],"for":[87,216],"6(S)-methyl,":[88],"6(R)-methyl,":[89],"6(S)-propyl,":[90],"and":[91,96,111,119,157,208],"6(R)-L-erythro-dihydroxypropyl":[92],"all":[93],"between":[94],"1.5":[95],"6":[97,194],"microM.":[98],"Turnover":[99],"numbers":[100],"at":[101,108,114],"37":[102],"degrees":[103],"C":[104],"are":[105,121,128,169],"50-90":[106],"s-1":[107],"pH":[109,115],"7.4":[110],"2.5-3-fold":[112],"lower":[113],"8.4.":[116],"Both":[117],"4a(R)-":[118],"4a(S)-hydroxytetrahydropterins":[120],"good":[122],"substrates.":[123],"quinoid":[125],"dihydropterin":[126],"products":[127],"strong":[129],"inhibitors":[130],"KI's":[135],"about":[136],"one":[137],"half":[138],"their":[140],"respective":[141],"Km's,":[142],"no":[144,155,158],"inhibition":[145],"observed":[147],"7,8-dihydropterins":[149],"tetrahydropterins.":[151],"contains":[154],"metals":[156],"phosphorus.":[159],"Reaction":[160],"mechanisms":[161],"which":[162],"involve":[163],"either":[164],"and/or":[166],"base":[167],"catalysis":[168],"discussed.":[170],"4a-Hydroxy-6(R)-tetrahydrobiopterin":[171],"determined":[173],"not":[174],"inhibitor":[179],"phenylalanine":[181],"hydroxylase.":[182],"It":[183],"concluded":[185],"that":[186],"(which":[189],"found":[191],"microM":[195],"in":[196,201],"liver)":[198],"essential":[200],"vivo":[202],"prevent":[204],"rearrangement":[205],"4a-hydroxy-6(R)-tetrahydrobiopterin":[207],"maintain":[210],"supply":[212],"hydroxylases":[218],"under":[219],"conditions":[220],"where":[221],"nonenzymatic":[223],"rate":[224],"would":[225],"inadequate.":[227]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}