{"id":"https://openalex.org/W2067239588","doi":"https://doi.org/10.1128/mcb.14.9.5731","title":"Ty1 In Vitro Integration: Effects of Mutations in cis and in trans","display_name":"Ty1 In Vitro Integration: Effects of Mutations in cis and in trans","publication_year":1994,"publication_date":"1994-09-01","ids":{"openalex":"https://openalex.org/W2067239588","doi":"https://doi.org/10.1128/mcb.14.9.5731","mag":"2067239588","pmid":"https://pubmed.ncbi.nlm.nih.gov/7520525","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/359098"},"language":"en","primary_location":{"id":"doi:10.1128/mcb.14.9.5731","doi":"https://doi.org/10.1128/mcb.14.9.5731","is_oa":false,"landing_page_url":"https://doi.org/10.1128/mcb.14.9.5731","pdf_url":null,"source":{"id":"https://openalex.org/S81455967","display_name":"Molecular and Cellular Biology","issn_l":"0270-7306","issn":["0270-7306","1067-8824","1098-5549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Cellular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/359098","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045068572","display_name":"Lelita T. Braiterman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L T Braiterman","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075536007","display_name":"Jef D. Boeke","orcid":"https://orcid.org/0000-0001-5322-4946","observed_orcids":["https://orcid.org/0000-0001-5322-4946"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J D Boeke","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0276,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.75080287,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"14","issue":"9","first_page":"5731","last_page":"5740"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.6416000127792358,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.6416000127792358,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.07500000298023224,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.05609999969601631,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/integrase","display_name":"integrase","score":0.8360000252723694},{"id":"https://openalex.org/keywords/frameshift-mutation","display_name":"frameshift mutation","score":0.9520000219345093},{"id":"https://openalex.org/keywords/missense-mutations","display_name":"missense mutations","score":0.9419999718666077},{"id":"https://openalex.org/keywords/c-terminal-domain","display_name":"C-terminal domain","score":0.6769999861717224},{"id":"https://openalex.org/keywords/retrovirus-like-particles","display_name":"retrovirus-like particles","score":0.5630000233650208},{"id":"https://openalex.org/keywords/transposon-insertion","display_name":"transposon insertion","score":0.3230000138282776}],"concepts":[{"id":"https://openalex.org/C29906990","wikidata":"https://www.wikidata.org/wiki/Q1440688","display_name":"Frameshift mutation","level":4,"score":0.8603516817092896},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8331490755081177},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.710521936416626},{"id":"https://openalex.org/C14430832","wikidata":"https://www.wikidata.org/wiki/Q412550","display_name":"Integrase","level":3,"score":0.5495726466178894},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5375131964683533},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.49271324276924133},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4562079608440399},{"id":"https://openalex.org/C75563809","wikidata":"https://www.wikidata.org/wiki/Q2656896","display_name":"Missense mutation","level":4,"score":0.45501983165740967},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4368698000907898},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.420118123292923},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3335275948047638}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009838","descriptor_name":"Oligodeoxyribonucleotides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012091","descriptor_name":"Repetitive Sequences, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012331","descriptor_name":"RNA, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/mcb.14.9.5731","doi":"https://doi.org/10.1128/mcb.14.9.5731","is_oa":false,"landing_page_url":"https://doi.org/10.1128/mcb.14.9.5731","pdf_url":null,"source":{"id":"https://openalex.org/S81455967","display_name":"Molecular and Cellular Biology","issn_l":"0270-7306","issn":["0270-7306","1067-8824","1098-5549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Cellular Biology","raw_type":"journal-article"},{"id":"doi:10.1128/mcb.14.9.5731-5740.1994","doi":"https://doi.org/10.1128/mcb.14.9.5731-5740.1994","is_oa":false,"landing_page_url":"https://doi.org/10.1128/mcb.14.9.5731-5740.1994","pdf_url":null,"source":{"id":"https://openalex.org/S81455967","display_name":"Molecular and Cellular Biology","issn_l":"0270-7306","issn":["0270-7306","1067-8824","1098-5549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Cellular Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:359098","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/359098","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Cell Biol","raw_type":"Text"},{"id":"pmid:7520525","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7520525","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and cellular biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:359098","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/359098","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Cell Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2606792740","display_name":"Graduate Research Fellowship Program","funder_award_id":"9154644","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G5432442139","display_name":"Transposition Mechanisms","funder_award_id":"R01GM036481","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W185246051","https://openalex.org/W640471480","https://openalex.org/W1483936602","https://openalex.org/W1569991285","https://openalex.org/W1572859712","https://openalex.org/W1602727746","https://openalex.org/W1602825981","https://openalex.org/W1621406154","https://openalex.org/W1638707894","https://openalex.org/W1738249403","https://openalex.org/W1753864123","https://openalex.org/W1819474806","https://openalex.org/W1960707077","https://openalex.org/W1965675983","https://openalex.org/W1966815449","https://openalex.org/W1971994381","https://openalex.org/W1973643623","https://openalex.org/W1979140780","https://openalex.org/W1983353035","https://openalex.org/W1995455922","https://openalex.org/W2000254114","https://openalex.org/W2006132678","https://openalex.org/W2014568295","https://openalex.org/W2017419757","https://openalex.org/W2021236910","https://openalex.org/W2022473619","https://openalex.org/W2025260589","https://openalex.org/W2026184248","https://openalex.org/W2041090656","https://openalex.org/W2043333510","https://openalex.org/W2050569727","https://openalex.org/W2058019531","https://openalex.org/W2059109354","https://openalex.org/W2070172523","https://openalex.org/W2084278833","https://openalex.org/W2089314092","https://openalex.org/W2089882643","https://openalex.org/W2110925400","https://openalex.org/W2125848967","https://openalex.org/W2135767314","https://openalex.org/W2151457726","https://openalex.org/W4244142180"],"related_works":["https://openalex.org/W2095779387","https://openalex.org/W1984124256","https://openalex.org/W2066743539","https://openalex.org/W1983353035","https://openalex.org/W2033408645","https://openalex.org/W2151457726","https://openalex.org/W2042717146","https://openalex.org/W2106967353","https://openalex.org/W2043333510","https://openalex.org/W2041090656"],"abstract_inverted_index":{"Mutations":[0],"within":[1,81,100],"the":[2,57,82,101,112,173],"TYB":[3],"gene":[4],"of":[5,59,87,105,127,132,188],"Ty1":[6,128,145],"encoding":[7],"integrase":[8],"(IN)":[9],"as":[10,12,50,122,124,162],"well":[11],"alterations":[13],"in":[14,27,90,92,168],"its":[15],"substrate,":[16],"a":[17,32,67],"linear":[18,153],"DNA":[19],"molecule,":[20],"were":[21,53,115,130,147,159,171],"examined":[22],"for":[23],"their":[24],"effects":[25],"on":[26],"vitro":[28,93],"IN":[29,75,88,94,106,109],"activity,":[30],"using":[31],"recently":[33],"developed":[34],"physical":[35],"assay.":[36],"Five":[37],"different":[38,63],"codon-insertion":[39],"mutations,":[40,43,52],"two":[41,62],"frameshift":[42,78],"and":[44,66,154],"one":[45],"missense":[46],"mutation,":[47],"previously":[48],"identified":[49],"transposition-deficient":[51],"tested.":[54],"Virus-like":[55],"particles,":[56],"source":[58],"IN,":[60],"from":[61],"protease":[64],"mutants":[65],"reverse":[68],"transcriptase":[69],"mutant":[70],"exhibited":[71],"near-normal":[72],"to":[73,144],"normal":[74],"activity.":[76,95,110],"Two":[77],"mutations":[79,99],"mapping":[80],"phylogenetically":[83],"variable":[84],"C-terminal":[85],"domain":[86,104],"resulted":[89],"significant":[91],"In":[96],"contrast,":[97],"three":[98],"amino-terminal":[102],"conserved":[103],"completely":[107],"abolished":[108],"When":[111],"substrate":[113],"termini":[114,129,146],"mutated,":[116],"we":[117],"found":[118],"that":[119,140],"substrates":[120,139,163,179],"with":[121],"few":[123],"4":[125],"bp":[126],"capable":[131],"efficiently":[133],"generating":[134],"integration":[135,189,193],"products.":[136],"Surprisingly,":[137],"certain":[138,178],"lacked":[141],"obvious":[142],"similarity":[143],"also":[148],"readily":[149],"integrated":[150],"into":[151],"both":[152],"circular":[155],"targets,":[156],"whereas":[157],"others":[158],"not":[160],"used":[161],"at":[164],"all.":[165],"Termini":[166],"rich":[167],"adenosine":[169,182],"residues":[170,183],"among":[172],"more":[174],"active":[175],"substrates;":[176],"however,":[177],"lacking":[180],"terminal":[181],"can":[184],"form":[185],"small":[186],"quantities":[187],"products,":[190],"including":[191],"complete":[192],"reactions.":[194]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}