{"id":"https://openalex.org/W2065899156","doi":"https://doi.org/10.1021/ac9509263","title":"Affinity Countercurrent Chromatography Using a Ligand in the Stationary Phase","display_name":"Affinity Countercurrent Chromatography Using a Ligand in the Stationary Phase","publication_year":1996,"publication_date":"1996-04-01","ids":{"openalex":"https://openalex.org/W2065899156","doi":"https://doi.org/10.1021/ac9509263","mag":"2065899156","pmid":"https://pubmed.ncbi.nlm.nih.gov/8651493"},"language":"en","primary_location":{"id":"doi:10.1021/ac9509263","doi":"https://doi.org/10.1021/ac9509263","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac9509263","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101493302","display_name":"Ying Ma","orcid":"https://orcid.org/0000-0002-1223-8177","observed_orcids":["https://orcid.org/0000-0002-1223-8177"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ying Ma","raw_affiliation_strings":["Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, 10 Center Drive MSC 1676, Bethesda, Maryland 20892-1676"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, 10 Center Drive MSC 1676, Bethesda, Maryland 20892-1676","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029128268","display_name":"Yoichiro Ito","orcid":"https://orcid.org/0000-0001-9018-295X","observed_orcids":["https://orcid.org/0000-0001-9018-295X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoichiro Ito","raw_affiliation_strings":["Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, 10 Center Drive MSC 1676, Bethesda, Maryland 20892-1676"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, 10 Center Drive MSC 1676, Bethesda, Maryland 20892-1676","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.9728,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.70512295,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"68","issue":"7","first_page":"1207","last_page":"1211"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.6797000169754028,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.6797000169754028,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.3142000138759613,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10180","display_name":"Analytical chemistry methods development","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bis-2-ethylhexyl-phosphoric-acid","display_name":"bis(2-ethylhexyl)phosphoric acid","score":0.9769999980926514},{"id":"https://openalex.org/keywords/amino-acid-derivatives","display_name":"amino acid derivatives","score":0.8479999899864197},{"id":"https://openalex.org/keywords/catecholamines","display_name":"catecholamines","score":0.9940000176429749},{"id":"https://openalex.org/keywords/dipeptides","display_name":"dipeptides","score":0.9860000014305115},{"id":"https://openalex.org/keywords/sample-loading-capacity","display_name":"sample loading capacity","score":0.9150000214576721},{"id":"https://openalex.org/keywords/retention-time","display_name":"retention time","score":0.4390000104904175},{"id":"https://openalex.org/keywords/peak-resolution","display_name":"peak resolution","score":0.9440000057220459}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9786038398742676},{"id":"https://openalex.org/C2983406500","wikidata":"https://www.wikidata.org/wiki/Q7604347","display_name":"Stationary phase","level":2,"score":0.746804416179657},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7015453577041626},{"id":"https://openalex.org/C63258622","wikidata":"https://www.wikidata.org/wiki/Q1498317","display_name":"Countercurrent chromatography","level":3,"score":0.6940937042236328},{"id":"https://openalex.org/C2778704781","wikidata":"https://www.wikidata.org/wiki/Q25303613","display_name":"Countercurrent distribution","level":2,"score":0.6254763007164001},{"id":"https://openalex.org/C187120659","wikidata":"https://www.wikidata.org/wiki/Q937912","display_name":"Countercurrent exchange","level":2,"score":0.602286159992218},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.5779655575752258},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.4891262650489807},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.44419580698013306},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.2648330628871918},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12333455681800842},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10262882709503174},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.09030541777610779},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.08890283107757568},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002395","descriptor_name":"Catecholamines","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003377","descriptor_name":"Countercurrent Distribution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004151","descriptor_name":"Dipeptides","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ac9509263","doi":"https://doi.org/10.1021/ac9509263","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac9509263","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:8651493","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8651493","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W619665994","https://openalex.org/W1994764690","https://openalex.org/W2029221908","https://openalex.org/W2032427297","https://openalex.org/W4241655348","https://openalex.org/W4244455167"],"related_works":["https://openalex.org/W44485747","https://openalex.org/W4239372454","https://openalex.org/W1668905142","https://openalex.org/W2083621227","https://openalex.org/W1974189880","https://openalex.org/W2110243128","https://openalex.org/W2059971705","https://openalex.org/W1520482620","https://openalex.org/W2090664477","https://openalex.org/W608065465"],"abstract_inverted_index":{"In":[0],"countercurrent":[1],"chromatography":[2],"(CCC),":[3],"an":[4,45,103],"addition":[5],"of":[6,22,64,76,91,99,110],"a":[7,62,88,92,124],"ligand":[8,47,78],"to":[9,67],"the":[10,23,52,56,77,80,97,100,116],"liquid":[11,53],"stationary":[12,54,94],"phase":[13,95],"remarkably":[14],"improved":[15],"both":[16],"retention":[17],"time":[18],"and":[19,36,48,72,113],"peak":[20],"resolution":[21],"analytes:":[24],"various":[25],"amino":[26],"acid":[27],"derivatives":[28],"were":[29,38],"separated":[30,39],"by":[31,40,86],"N-dodecanoyl-L-proline-3,5-dimethylanilide,":[32],"while":[33],"polar":[34],"catecholamines":[35],"dipeptides":[37],"bis-(2-ethylhexyl)phosphoric":[41],"acid.":[42],"By":[43],"selecting":[44],"appropriate":[46],"dissolving":[49],"it":[50],"in":[51],"phase,":[55],"present":[57],"CCC":[58,112],"technique":[59],"can":[60,82,114],"perform":[61],"variety":[63],"separations":[65],"comparable":[66],"chiral":[68],"chromatography,":[69,71],"ion":[70],"affinity":[73],"chromatography.":[74],"Leakage":[75],"from":[79],"column":[81,101],"be":[83],"entirely":[84],"eliminated":[85],"introducing":[87],"small":[89],"volume":[90],"ligand-free":[93],"at":[96],"end":[98],"as":[102],"absorbent.":[104],"The":[105],"method":[106],"further":[107],"facilitates":[108],"application":[109],"pH-zone-refining":[111],"increase":[115],"sample":[117],"loading":[118],"capacity":[119],"over":[120],"10":[121],"times":[122],"for":[123],"given":[125],"column.":[126]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}