{"id":"https://openalex.org/W2065629778","doi":"https://doi.org/10.1371/journal.pone.0077195","title":"1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture","display_name":"1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture","publication_year":2013,"publication_date":"2013-10-10","ids":{"openalex":"https://openalex.org/W2065629778","doi":"https://doi.org/10.1371/journal.pone.0077195","mag":"2065629778","pmid":"https://pubmed.ncbi.nlm.nih.gov/24130854","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3795012"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0077195","doi":"https://doi.org/10.1371/journal.pone.0077195","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0077195","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0077195&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0077195&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113962052","display_name":"Jane L. Wagstaff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jane L. Wagstaff","raw_affiliation_strings":["Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008673050","display_name":"Rosalyn J. Masterton","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Rosalyn J. Masterton","raw_affiliation_strings":["Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050367368","display_name":"Jane F. Povey","orcid":"https://orcid.org/0000-0003-4522-3630","observed_orcids":["https://orcid.org/0000-0003-4522-3630"]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jane F. Povey","raw_affiliation_strings":["Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017406022","display_name":"Christopher Mark Smales","orcid":"https://orcid.org/0000-0002-2762-4724","observed_orcids":["https://orcid.org/0000-0002-2762-4724"]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"C. Mark Smales","raw_affiliation_strings":["Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065412120","display_name":"Mark J. Howard","orcid":"https://orcid.org/0000-0002-0762-2887","observed_orcids":["https://orcid.org/0000-0002-0762-2887"]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Mark J. Howard","raw_affiliation_strings":["Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Molecular Processing and School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom","institution_ids":["https://openalex.org/I20581793"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5017406022","https://openalex.org/A5065412120"],"corresponding_institution_ids":["https://openalex.org/I20581793"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":0.9898,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.73041115,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"8","issue":"10","first_page":"e77195","last_page":"e77195"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8935999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8935999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.09380000084638596,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metabolic-reprogramming","display_name":"metabolic reprogramming","score":0.9779999852180481},{"id":"https://openalex.org/keywords/cold-shock","display_name":"cold shock","score":0.7730000019073486},{"id":"https://openalex.org/keywords/temperature-change","display_name":"temperature change","score":0.7739999890327454},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.878000020980835},{"id":"https://openalex.org/keywords/cho-s-cells","display_name":"CHO-S cells","score":0.6679999828338623},{"id":"https://openalex.org/keywords/extracellular-metabolites","display_name":"extracellular metabolites","score":0.777999997138977},{"id":"https://openalex.org/keywords/lactate","display_name":"lactate","score":0.8199999928474426},{"id":"https://openalex.org/keywords/glucose","display_name":"glucose","score":0.9359999895095825},{"id":"https://openalex.org/keywords/alanine","display_name":"alanine","score":0.9779999852180481},{"id":"https://openalex.org/keywords/culture-medium-composition","display_name":"culture medium composition","score":0.382999986410141}],"concepts":[{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.8104134202003479},{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.7473633885383606},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.7164968848228455},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6546701192855835},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5555016398429871},{"id":"https://openalex.org/C77255625","wikidata":"https://www.wikidata.org/wiki/Q402093","display_name":"Reprogramming","level":3,"score":0.5498389005661011},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5488933324813843},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5133423209190369},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.4786616265773773},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45168787240982056},{"id":"https://openalex.org/C2994212657","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Cellular metabolism","level":3,"score":0.4430132210254669},{"id":"https://openalex.org/C160941953","wikidata":"https://www.wikidata.org/wiki/Q6822342","display_name":"Metabolic flux analysis","level":3,"score":0.424968421459198},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.41677138209342957},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.4138736128807068},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.396888792514801},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3517884910106659},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.33209481835365295},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.12372612953186035},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08259305357933044}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010053","descriptor_name":"Ovary","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019344","descriptor_name":"Lactic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D042541","descriptor_name":"Intracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1371/journal.pone.0077195","doi":"https://doi.org/10.1371/journal.pone.0077195","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0077195","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0077195&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2788122","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3795012","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3795012","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3795012","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:24130854","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24130854","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:kar.kent.ac.uk:35491","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0077195>)","pdf_url":"https://kar.kent.ac.uk/35491/1/1HNMR_CHOK1CHOS_PLoSOne_2013.pdf","source":{"id":"https://openalex.org/S4377196264","display_name":"Kent Academic Repository (University of Kent)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I20581793","host_organization_name":"University of Kent","host_organization_lineage":["https://openalex.org/I20581793"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.690.2188","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.690.2188","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795012/pdf/pone.0077195.pdf","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:64baea31c61b40278979c1b15473d237","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/64baea31c61b40278979c1b15473d237","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 8, Iss 10, p e77195 (2013)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0077195","doi":"https://doi.org/10.1371/journal.pone.0077195","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0077195","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0077195&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2069622432","display_name":null,"funder_award_id":"BB/C006569/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G3741362164","display_name":"Defining novel mechanisms of mRNA translational control upon cold-shock in mammalian cells","funder_award_id":"BB/I020055/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G3898476380","display_name":"Towards the manipulation of the UPR in mammalian cells to orchestrate cellular re-organization for enhanced monoclonal antibody production","funder_award_id":"BB/D009375/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G7762551667","display_name":null,"funder_award_id":"BB/D009375/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320320666","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2065629778.pdf","grobid_xml":"https://content.openalex.org/works/W2065629778.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W1188919047","https://openalex.org/W1573796272","https://openalex.org/W1577332351","https://openalex.org/W1976552586","https://openalex.org/W1979668518","https://openalex.org/W1986406786","https://openalex.org/W2028240481","https://openalex.org/W2043139382","https://openalex.org/W2048354832","https://openalex.org/W2067210466","https://openalex.org/W2071412002","https://openalex.org/W2084104744","https://openalex.org/W2091239325","https://openalex.org/W2093999909","https://openalex.org/W2094337087","https://openalex.org/W2104694760","https://openalex.org/W2111660101","https://openalex.org/W2117045376","https://openalex.org/W2118252985","https://openalex.org/W2118540273","https://openalex.org/W2128571608","https://openalex.org/W2131215809","https://openalex.org/W2152680042"],"related_works":["https://openalex.org/W2084104744","https://openalex.org/W2017001477","https://openalex.org/W2156639989","https://openalex.org/W2099681746","https://openalex.org/W2792352360","https://openalex.org/W3014719705","https://openalex.org/W4392256482","https://openalex.org/W2201464011","https://openalex.org/W2617872771","https://openalex.org/W1986406786"],"abstract_inverted_index":{"We":[0,86],"report":[1],"an":[2,89],"NMR":[3,90,173],"based":[4],"approach":[5,92,168],"to":[6,42,114,136,162,177,192,200],"determine":[7],"the":[8,24,68,76,170,190],"metabolic":[9,91],"reprogramming":[10,131,179],"of":[11,32,52,78,80,96,120,132,144,172,180,185,195],"Chinese":[12],"hamster":[13],"ovary":[14],"cells":[15,36],"upon":[16,182],"a":[17,109,123],"temperature":[18,137,163],"shift":[19],"during":[20,37],"culture":[21,27,38,198],"by":[22],"investigating":[23],"extracellular":[25],"cell":[26,65,105,139],"media":[28,72,82],"and":[29,34,39,44,59,101,112,127,129,164],"intracellular":[30],"metabolome":[31],"CHOK1":[33,58,81,126],"CHO-S":[35,64,71,128],"in":[40,108,134,157,160,197],"response":[41,135,161],"cold-shock":[43],"subsequent":[45],"recovery":[46],"from":[47],"hypothermic":[48],"culturing.":[49],"A":[50],"total":[51],"24":[53],"components":[54,61,97],"were":[55],"identified":[56,62],"for":[57,63],"29":[60],"systems":[66],"including":[67],"observation":[69],"that":[70,88,130,154],"contains":[73],"5.6":[74],"times":[75],"level":[77],"glucose":[79,100],"at":[83],"time":[84],"zero.":[85],"confirm":[87],"provides":[93],"quantitative":[94],"analysis":[95,119,152,176],"such":[98],"as":[99],"alanine":[102],"with":[103],"both":[104],"lines":[106],"responding":[107],"similar":[110],"manner":[111],"comparable":[113],"previously":[115],"reported":[116],"data.":[117],"However,":[118],"lactate":[121],"confirms":[122,155],"differentiation":[124],"between":[125],"metabolism":[133,181],"was":[138],"line":[140],"specific.":[141],"The":[142],"significance":[143],"our":[145,167],"results":[146],"is":[147],"presented":[148],"using":[149],"principal":[150],"component":[151],"(PCA)":[153],"changes":[156],"metabolite":[158],"profile":[159],"recovery.":[165],"Ultimately,":[166],"demonstrates":[169],"capability":[171],"providing":[174],"real-time":[175],"detect":[178],"cellular":[183],"perception":[184],"cold-shock/sub-physiological":[186],"temperatures.":[187],"This":[188],"has":[189],"potential":[191],"allow":[193],"manipulation":[194],"metabolites":[196],"supernatant":[199],"improve":[201],"growth":[202],"or":[203],"productivity.":[204]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}