{"id":"https://openalex.org/W2065369136","doi":"https://doi.org/10.1271/bbb.60366","title":"Rapid Quantification Methods for Genetically Modified Maize Contents Using Genomic DNAs Pretreated by Sonication and Restriction Endonuclease Digestion for a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard","display_name":"Rapid Quantification Methods for Genetically Modified Maize Contents Using Genomic DNAs Pretreated by Sonication and Restriction Endonuclease Digestion for a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard","publication_year":2006,"publication_date":"2006-12-23","ids":{"openalex":"https://openalex.org/W2065369136","doi":"https://doi.org/10.1271/bbb.60366","mag":"2065369136","pmid":"https://pubmed.ncbi.nlm.nih.gov/17151472"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.60366","doi":"https://doi.org/10.1271/bbb.60366","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60366","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061990259","display_name":"Akie Toyota","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113306721","display_name":"Hiroshima University","ror":"https://ror.org/03t78wx29","country_code":"JP","type":"education","lineage":["https://openalex.org/I113306721"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akie TOYOTA","raw_affiliation_strings":["Hiroshima Prefectural Institute of Public Health and Environment, Hiroshima","Graduate School of Biosphere Sciences, Hiroshima University","Hiroshima Prefectural Institute of Public Health and Environment"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hiroshima Prefectural Institute of Public Health and Environment, Hiroshima","institution_ids":[]},{"raw_affiliation_string":"Graduate School of Biosphere Sciences, Hiroshima University","institution_ids":["https://openalex.org/I113306721"]},{"raw_affiliation_string":"Hiroshima Prefectural Institute of Public Health and Environment","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090659492","display_name":"Hiroshi Akiyama","orcid":"https://orcid.org/0000-0002-7764-8601","observed_orcids":["https://orcid.org/0000-0002-7764-8601"]},"institutions":[{"id":"https://openalex.org/I4210151964","display_name":"National Institute of Health Sciences","ror":"https://ror.org/04s629c33","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4210151964"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroshi AKIYAMA","raw_affiliation_strings":["National Institute of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Health Sciences","institution_ids":["https://openalex.org/I4210151964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003249456","display_name":"Mitsunori SUGIMURA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mitsunori SUGIMURA","raw_affiliation_strings":["Hiroshima Prefectural Institute of Public Health and Environment"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hiroshima Prefectural Institute of Public Health and Environment","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079132507","display_name":"Takahiro Watanabe","orcid":"https://orcid.org/0000-0002-7249-8106","observed_orcids":["https://orcid.org/0000-0002-7249-8106"]},"institutions":[{"id":"https://openalex.org/I4210151964","display_name":"National Institute of Health Sciences","ror":"https://ror.org/04s629c33","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4210151964"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takahiro WATANABE","raw_affiliation_strings":["National Institute of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Health Sciences","institution_ids":["https://openalex.org/I4210151964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111575967","display_name":"Kozue Sakata","orcid":"https://orcid.org/0000-0002-3086-0955","observed_orcids":["https://orcid.org/0000-0002-3086-0955"]},"institutions":[{"id":"https://openalex.org/I4210151964","display_name":"National Institute of Health Sciences","ror":"https://ror.org/04s629c33","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4210151964"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kozue SAKATA","raw_affiliation_strings":["National Institute of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Health Sciences","institution_ids":["https://openalex.org/I4210151964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045708346","display_name":"Yuko Shiramasa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210151964","display_name":"National Institute of Health Sciences","ror":"https://ror.org/04s629c33","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4210151964"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuko SHIRAMASA","raw_affiliation_strings":["National Institute of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Health Sciences","institution_ids":["https://openalex.org/I4210151964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078885047","display_name":"Kazumi Kitta","orcid":"https://orcid.org/0000-0002-3691-271X","observed_orcids":["https://orcid.org/0000-0002-3691-271X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kazumi KITTA","raw_affiliation_strings":["National Food Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Food Research Institute","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109065349","display_name":"Akihiro Hino","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Akihiro HINO","raw_affiliation_strings":["National Food Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Food Research Institute","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032533452","display_name":"Muneharu Esaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113306721","display_name":"Hiroshima University","ror":"https://ror.org/03t78wx29","country_code":"JP","type":"education","lineage":["https://openalex.org/I113306721"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Muneharu ESAKA","raw_affiliation_strings":["Graduate School of Biosphere Sciences, Hiroshima University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Biosphere Sciences, Hiroshima University","institution_ids":["https://openalex.org/I113306721"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034836717","display_name":"Tamio Maitani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210151964","display_name":"National Institute of Health Sciences","ror":"https://ror.org/04s629c33","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4210151964"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tamio MAITANI","raw_affiliation_strings":["National Institute of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Health Sciences","institution_ids":["https://openalex.org/I4210151964"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":1.2776,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.81302063,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"70","issue":"12","first_page":"2965","last_page":"2973"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transgenic-maize","display_name":"transgenic maize","score":0.8550000190734863},{"id":"https://openalex.org/keywords/mon810","display_name":"MON810","score":0.9139999747276306},{"id":"https://openalex.org/keywords/camv-35s-promoter","display_name":"CaMV 35S promoter","score":0.9169999957084656},{"id":"https://openalex.org/keywords/sonication","display_name":"sonication","score":0.9980000257492065},{"id":"https://openalex.org/keywords/restriction-enzyme-digestion","display_name":"restriction enzyme digestion","score":0.9390000104904175},{"id":"https://openalex.org/keywords/gmo-detection","display_name":"GMO detection","score":0.4449999928474426}],"concepts":[{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.692816972732544},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6446951031684875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5876517295837402},{"id":"https://openalex.org/C154615088","wikidata":"https://www.wikidata.org/wiki/Q1363424","display_name":"Genetically modified maize","level":5,"score":0.5392834544181824},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5371904969215393},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4983363151550293},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.4862591028213501},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4537818431854248},{"id":"https://openalex.org/C2780037811","wikidata":"https://www.wikidata.org/wiki/Q430416","display_name":"Cauliflower mosaic virus","level":5,"score":0.4136631190776825},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.41194507479667664},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.40956413745880127},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.39257150888442993},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3651258945465088},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.28461331129074097},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26612964272499084},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.16239210963249207},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.09476602077484131}],"mesh":[{"descriptor_ui":"D003313","descriptor_name":"Zea mays","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012015","descriptor_name":"Reference Standards","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018744","descriptor_name":"DNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.60366","doi":"https://doi.org/10.1271/bbb.60366","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.60366","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:17151472","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17151472","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.8409000039100647}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W198961861","https://openalex.org/W381291367","https://openalex.org/W1481199738","https://openalex.org/W1502671206","https://openalex.org/W1517821097","https://openalex.org/W1560977078","https://openalex.org/W1963811106","https://openalex.org/W1966894552","https://openalex.org/W1980227479","https://openalex.org/W1982309075","https://openalex.org/W1986168612","https://openalex.org/W2014276654","https://openalex.org/W2024392840","https://openalex.org/W2025766910","https://openalex.org/W2026633372","https://openalex.org/W2029497249","https://openalex.org/W2031078743","https://openalex.org/W2036288731","https://openalex.org/W2037611845","https://openalex.org/W2040570473","https://openalex.org/W2041398172","https://openalex.org/W2052568614","https://openalex.org/W2058450752","https://openalex.org/W2073283075","https://openalex.org/W2084940897","https://openalex.org/W2086767221","https://openalex.org/W2100141768","https://openalex.org/W2130299820","https://openalex.org/W2138287829","https://openalex.org/W2155075188","https://openalex.org/W2171498281","https://openalex.org/W2205582801"],"related_works":["https://openalex.org/W2130299820","https://openalex.org/W7098867109","https://openalex.org/W2067357476","https://openalex.org/W2406027215","https://openalex.org/W2006422810","https://openalex.org/W2156012944","https://openalex.org/W2053646193","https://openalex.org/W2903242659","https://openalex.org/W2039109393","https://openalex.org/W2135076295"],"abstract_inverted_index":{"For":[0],"rough":[1],"quantitative":[2],"analysis":[3],"of":[4,13,17,34,62,70,84,94],"genetically":[5],"modified":[6],"maize":[7,126],"contents,":[8],"rapid":[9],"methods":[10,114],"for":[11,87],"measurement":[12,77],"the":[14,18,48,52,63,108],"copy":[15,110],"numbers":[16],"cauliflower":[19],"mosaic":[20],"virus":[21],"35S":[22],"promoter":[23],"region":[24],"(P35S)":[25],"and":[26,55,72,82],"MON810":[27,88,96],"construct-specific":[28],"gene":[29],"(MON810)":[30],"using":[31],"a":[32,35,41,68,92,101,119],"combination":[33,69],"capillary-type":[36],"real-time":[37],"PCR":[38],"system":[39],"with":[40],"plasmid":[42,53],"DNA":[43,54,66],"were":[44,99],"established.":[45],"To":[46],"reduce":[47],"characteristic":[49],"differences":[50],"between":[51],"genomic":[56,65],"DNA,":[57],"we":[58],"showed":[59],"that":[60],"pretreatment":[61],"extracted":[64],"by":[67],"sonication":[71],"restriction":[73],"endonuclease":[74],"digestion":[75],"before":[76],"is":[78],"effective.":[79],"The":[80],"accuracy":[81],"reproducibility":[83],"this":[85],"method":[86],"content":[89],"(%)":[90],"at":[91],"level":[93],"5.0%":[95],"mixed":[97],"samples":[98],"within":[100],"range":[102],"from":[103],"4.26":[104],"to":[105,117,122],"5.11%":[106],"in":[107],"P35S":[109],"number":[111],"quantification.":[112],"These":[113],"should":[115],"prove":[116],"be":[118],"useful":[120],"tool":[121],"roughly":[123],"quantify":[124],"GM":[125],"content.":[127]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}