{"id":"https://openalex.org/W2064468792","doi":"https://doi.org/10.1021/bm2015174","title":"Design and Biophysical Characterization of Bioresponsive Degradable Poly(dimethylaminoethyl methacrylate) Based Polymers for In Vitro DNA Transfection","display_name":"Design and Biophysical Characterization of Bioresponsive Degradable Poly(dimethylaminoethyl methacrylate) Based Polymers for In Vitro DNA Transfection","publication_year":2011,"publication_date":"2011-12-22","ids":{"openalex":"https://openalex.org/W2064468792","doi":"https://doi.org/10.1021/bm2015174","mag":"2064468792","pmid":"https://pubmed.ncbi.nlm.nih.gov/22191470"},"language":"en","primary_location":{"id":"doi:10.1021/bm2015174","doi":"https://doi.org/10.1021/bm2015174","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bm2015174","pdf_url":null,"source":{"id":"https://openalex.org/S11629336","display_name":"Biomacromolecules","issn_l":"1525-7797","issn":["1525-7797","1526-4602"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Yi Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Yi Zhang","raw_affiliation_strings":["Department of Chemistry\rand Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein\rStrasse, 35032 Marburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry\rand Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein\rStrasse, 35032 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046806058","display_name":"Mengyao Zheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Mengyao Zheng","raw_affiliation_strings":["Department of Pharmaceutics\rand Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics\rand Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017318583","display_name":"Thomas Kissel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Thomas Kissel","raw_affiliation_strings":["Department of Chemistry and Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein Strasse, 35032 Marburg, Germany","Department of Pharmaceutics and Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein Strasse, 35032 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]},{"raw_affiliation_string":"Department of Pharmaceutics and Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052481664","display_name":"Seema Agarwal","orcid":"https://orcid.org/0000-0002-3174-3152","observed_orcids":["https://orcid.org/0000-0002-3174-3152"]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Seema Agarwal","raw_affiliation_strings":["Department of Chemistry and Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein Strasse, 35032 Marburg, Germany","Department of Pharmaceutics and Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Scientific Center for Materials Science, Philipps-Universit\u00e4t Marburg, Hans-Meerwein Strasse, 35032 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]},{"raw_affiliation_string":"Department of Pharmaceutics and Biopharmacy, Philipps-Universit\u00e4t Marburg, Ketzerbach 63, 35037 Marburg, Germany","institution_ids":["https://openalex.org/I161103922"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5017318583","https://openalex.org/A5052481664"],"corresponding_institution_ids":["https://openalex.org/I161103922"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.5679,"has_fulltext":false,"cited_by_count":62,"citation_normalized_percentile":{"value":0.90738355,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"13","issue":"2","first_page":"313","last_page":"322"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9340999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9340999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10405","display_name":"Advanced Polymer Synthesis and Characterization","score":0.014600000344216824,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11686","display_name":"Dendrimers and Hyperbranched Polymers","score":0.009999999776482582,"subfield":{"id":"https://openalex.org/subfields/2507","display_name":"Polymers and Plastics"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pdmaema","display_name":"PDMAEMA","score":0.9380000233650208},{"id":"https://openalex.org/keywords/degradable-polymers","display_name":"degradable polymers","score":0.800000011920929},{"id":"https://openalex.org/keywords/transfection","display_name":"transfection","score":0.8040000200271606},{"id":"https://openalex.org/keywords/dna-transfection","display_name":"DNA transfection","score":0.7390000224113464},{"id":"https://openalex.org/keywords/biodegradable-copolymers","display_name":"biodegradable copolymers","score":0.7279999852180481},{"id":"https://openalex.org/keywords/pegylation","display_name":"PEGylation","score":0.925000011920929},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.8679999709129333},{"id":"https://openalex.org/keywords/water-solubility","display_name":"water solubility","score":0.8140000104904175},{"id":"https://openalex.org/keywords/quaternization","display_name":"quaternization","score":0.8149999976158142},{"id":"https://openalex.org/keywords/l929-fibroblasts","display_name":"L929 fibroblasts","score":0.8799999952316284}],"concepts":[{"id":"https://openalex.org/C132050475","wikidata":"https://www.wikidata.org/wiki/Q736232","display_name":"Methacrylate","level":4,"score":0.7911463379859924},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.7040143013000488},{"id":"https://openalex.org/C15920480","wikidata":"https://www.wikidata.org/wiki/Q421281","display_name":"Copolymer","level":3,"score":0.6692749261856079},{"id":"https://openalex.org/C2909430059","wikidata":"https://www.wikidata.org/wiki/Q409141","display_name":"Ethyleneimine","level":3,"score":0.6672844290733337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6645073294639587},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5797645449638367},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.5477643609046936},{"id":"https://openalex.org/C155574463","wikidata":"https://www.wikidata.org/wiki/Q170731","display_name":"Solubility","level":2,"score":0.5007855892181396},{"id":"https://openalex.org/C2777801237","wikidata":"https://www.wikidata.org/wiki/Q407473","display_name":"Ethylene oxide","level":4,"score":0.4893648028373718},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.43238401412963867},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.3273223638534546},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.16084697842597961},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10693898797035217},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001672","descriptor_name":"Biocompatible Materials","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D001672","descriptor_name":"Biocompatible Materials","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008689","descriptor_name":"Methacrylates","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D008689","descriptor_name":"Methacrylates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009757","descriptor_name":"Nylons","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D009757","descriptor_name":"Nylons","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010316","descriptor_name":"Particle Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011094","descriptor_name":"Polyethyleneimine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bm2015174","doi":"https://doi.org/10.1021/bm2015174","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bm2015174","pdf_url":null,"source":{"id":"https://openalex.org/S11629336","display_name":"Biomacromolecules","issn_l":"1525-7797","issn":["1525-7797","1526-4602"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"journal-article"},{"id":"pmid:22191470","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22191470","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W53334991","https://openalex.org/W304669699","https://openalex.org/W594662588","https://openalex.org/W1966782476","https://openalex.org/W1976401343","https://openalex.org/W1976556354","https://openalex.org/W1979443647","https://openalex.org/W1981770322","https://openalex.org/W1997993853","https://openalex.org/W2007872410","https://openalex.org/W2014659193","https://openalex.org/W2031632278","https://openalex.org/W2035051990","https://openalex.org/W2041814283","https://openalex.org/W2050932091","https://openalex.org/W2052040755","https://openalex.org/W2058006748","https://openalex.org/W2060950062","https://openalex.org/W2074133546","https://openalex.org/W2078377919","https://openalex.org/W2080688395","https://openalex.org/W2086847600","https://openalex.org/W2091211551","https://openalex.org/W2091813929","https://openalex.org/W2095268526","https://openalex.org/W2107464363","https://openalex.org/W2114918609","https://openalex.org/W2116461835","https://openalex.org/W2128317669","https://openalex.org/W2129399211","https://openalex.org/W2136239884","https://openalex.org/W2160064249","https://openalex.org/W2889149062"],"related_works":["https://openalex.org/W2162664297","https://openalex.org/W2945527386","https://openalex.org/W2025471675","https://openalex.org/W1972273221","https://openalex.org/W2070251217","https://openalex.org/W2043764618","https://openalex.org/W1981304130","https://openalex.org/W1977047057","https://openalex.org/W2331947347","https://openalex.org/W3212716337"],"abstract_inverted_index":{"Water-soluble,":[0],"degradable":[1,89,124],"polymers":[2,29,67,125],"based":[3,30,123],"on":[4,31],"poly(N,N-dimethylaminoethyl":[5],"methacrylate)":[6],"(PDMAEMA)":[7],"with":[8,81,95,111],"low":[9],"cytotoxicity":[10,61],"and":[11,33,58,75,103,144],"good":[12],"p-DNA":[13],"transfection":[14,38,107,143],"efficiency":[15,108],"are":[16],"highlighted":[17],"in":[18,72,78,141],"this":[19],"article.":[20],"To":[21],"solve":[22],"the":[23,63,73,112,119,130,149],"nondegradability":[24],"issue":[25],"of":[26,62,85,118],"PDMAEMA,":[27],"new":[28],"DMAEMA":[32,122,132],"5,6-benzo-2-methylene-1,3-dioxepane":[34],"(BMDO)":[35],"for":[36],"gene":[37,142],"were":[39,76,126,139],"synthesized.":[40],"A":[41],"poly(ethylene":[42],"oxide)":[43],"(PEO)":[44],"azo-initiator":[45],"was":[46,52],"used":[47],"as":[48,146],"free-radical":[49],"initiator.":[50],"PEGylation":[51],"performed":[53],"to":[54,59,148],"improve":[55],"water":[56,79,136],"solubility":[57],"reduce":[60],"polymers.":[64],"The":[65],"resulting":[66],"contain":[68],"hydrolyzable":[69],"ester":[70,86],"linkages":[71],"backbone":[74],"soluble":[77],"even":[80],"very":[82],"high":[83],"amounts":[84],"linkages.":[87],"These":[88],"copolymers":[90,133],"showed":[91,134],"significantly":[92],"less":[93],"toxicity":[94,145],"a":[96],"MTT":[97],"assay":[98],"using":[99],"L929":[100],"cell":[101],"lines":[102],"demonstrated":[104],"promising":[105],"DNA":[106],"when":[109],"compared":[110,147],"gold":[113],"standard":[114],"poly(ethyleneimine).":[115],"Bioresponsive":[116],"properties":[117],"corresponding":[120],"quaternized":[121,131],"also":[127],"studied.":[128],"Although":[129],"enhanced":[135],"solubility,":[137],"they":[138],"inferior":[140],"unquaternized":[150],"copolymers.":[151]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":11},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}