{"id":"https://openalex.org/W2064466849","doi":"https://doi.org/10.1074/jbc.m300611200","title":"Resistance to \u03b2-Lactam Antibiotics and Its Mediation by the Sensor Domain of the Transmembrane BlaR Signaling Pathway in Staphylococcus aureus","display_name":"Resistance to \u03b2-Lactam Antibiotics and Its Mediation by the Sensor Domain of the Transmembrane BlaR Signaling Pathway in Staphylococcus aureus","publication_year":2003,"publication_date":"2003-05-01","ids":{"openalex":"https://openalex.org/W2064466849","doi":"https://doi.org/10.1074/jbc.m300611200","mag":"2064466849","pmid":"https://pubmed.ncbi.nlm.nih.gov/12591921"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m300611200","doi":"https://doi.org/10.1074/jbc.m300611200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m300611200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m300611200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086433612","display_name":"Dasantila Golemi","orcid":"https://orcid.org/0000-0003-1299-6987","observed_orcids":["https://orcid.org/0000-0003-1299-6987"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dasantila Golemi-Kotra","raw_affiliation_strings":["Department of Chemistry, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489, USA","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047301389","display_name":"Joo Young Cha","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joo Young Cha","raw_affiliation_strings":["Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]},{"raw_affiliation_string":"From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055133888","display_name":"Samy O. Meroueh","orcid":"https://orcid.org/0000-0002-3948-7499","observed_orcids":["https://orcid.org/0000-0002-3948-7499"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samy O. Meroueh","raw_affiliation_strings":["Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]},{"raw_affiliation_string":"From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043351423","display_name":"Sergei B. Vakulenko","orcid":"https://orcid.org/0000-0002-5327-8124","observed_orcids":["https://orcid.org/0000-0002-5327-8124"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sergei B. Vakulenko","raw_affiliation_strings":["Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]},{"raw_affiliation_string":"From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013812375","display_name":"Shahriar Mobashery","orcid":"https://orcid.org/0000-0002-7695-7883","observed_orcids":["https://orcid.org/0000-0002-7695-7883"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Shahriar Mobashery","raw_affiliation_strings":["Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]},{"raw_affiliation_string":"From the Departments of Chemistry, of Pharmacology, and of Biochemistry and Molecular Biology, Institute for Drug Design, Wayne State University, Detroit, Michigan 48202-3489","institution_ids":["https://openalex.org/I185443292"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013812375"],"corresponding_institution_ids":["https://openalex.org/I185443292"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.4688,"has_fulltext":false,"cited_by_count":82,"citation_normalized_percentile":{"value":0.93392672,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"278","issue":"20","first_page":"18419","last_page":"18425"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.042500000447034836,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.039400000125169754,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-lactam-resistance","display_name":"beta-lactam resistance","score":0.8349999785423279},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.9419999718666077},{"id":"https://openalex.org/keywords/beta-lactamase-induction","display_name":"beta-lactamase induction","score":0.7369999885559082},{"id":"https://openalex.org/keywords/pbp2a","display_name":"PBP2a","score":0.8259999752044678},{"id":"https://openalex.org/keywords/penicillin-binding-protein-2a","display_name":"penicillin-binding protein 2a","score":0.8230000138282776},{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.8289999961853027},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.8690000176429749}],"concepts":[{"id":"https://openalex.org/C2776653525","wikidata":"https://www.wikidata.org/wiki/Q7162855","display_name":"Penicillin binding proteins","level":4,"score":0.6960400342941284},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.6242150068283081},{"id":"https://openalex.org/C2776634448","wikidata":"https://www.wikidata.org/wiki/Q206920","display_name":"Peptidoglycan","level":3,"score":0.5012164115905762},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.48957905173301697},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4608793556690216},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4448431432247162},{"id":"https://openalex.org/C174802600","wikidata":"https://www.wikidata.org/wiki/Q12190","display_name":"Penicillin","level":3,"score":0.4430885314941406},{"id":"https://openalex.org/C2776131168","wikidata":"https://www.wikidata.org/wiki/Q251124","display_name":"Lactam","level":2,"score":0.41877979040145874},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3792566955089569},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23636749386787415},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.12471598386764526}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002244","descriptor_name":"Carbon","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002245","descriptor_name":"Carbon Dioxide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002267","descriptor_name":"Muramoylpentapeptide Carboxypeptidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004352","descriptor_name":"Drug Resistance, Microbial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006602","descriptor_name":"Hexosyltransferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010458","descriptor_name":"Peptidyl Transferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012694","descriptor_name":"Serine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046915","descriptor_name":"Penicillin-Binding Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D047090","descriptor_name":"beta-Lactams","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m300611200","doi":"https://doi.org/10.1074/jbc.m300611200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m300611200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:12591921","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12591921","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m300611200","doi":"https://doi.org/10.1074/jbc.m300611200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m300611200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1060476269","display_name":"Cell-Wall Recycling and Antibiotic Resistance","funder_award_id":"R01GM061629","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G2614247043","display_name":null,"funder_award_id":"GM 61629","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G420529085","display_name":null,"funder_award_id":"AI331790","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W73473199","https://openalex.org/W1523409504","https://openalex.org/W1527353036","https://openalex.org/W1581506296","https://openalex.org/W1585468492","https://openalex.org/W1869944834","https://openalex.org/W1970864487","https://openalex.org/W1976499671","https://openalex.org/W2008841836","https://openalex.org/W2015105695","https://openalex.org/W2039388361","https://openalex.org/W2046425440","https://openalex.org/W2053004995","https://openalex.org/W2055043387","https://openalex.org/W2057958258","https://openalex.org/W2079988639","https://openalex.org/W2080689402","https://openalex.org/W2083021997","https://openalex.org/W2085514955","https://openalex.org/W2090505190","https://openalex.org/W2092196558","https://openalex.org/W2114533737","https://openalex.org/W2128577323","https://openalex.org/W2144147504","https://openalex.org/W2148663866","https://openalex.org/W2171952306","https://openalex.org/W2344454939","https://openalex.org/W2903140909"],"related_works":["https://openalex.org/W2044124840","https://openalex.org/W2082177591","https://openalex.org/W7096947869","https://openalex.org/W2011445171","https://openalex.org/W2091069899","https://openalex.org/W2051475806","https://openalex.org/W1988431861","https://openalex.org/W4313545533","https://openalex.org/W2064771945","https://openalex.org/W1596557611"],"abstract_inverted_index":{"Staphylococci,":[0],"a":[1,148,184,193],"leading":[2],"cause":[3],"of":[4,19,30,85,90,138,141,143,150,167],"infections":[5],"worldwide,":[6],"have":[7],"devised":[8],"two":[9],"mechanisms":[10],"for":[11,72,81,130],"resistance":[12,22],"to":[13,40,64,100,117,217,220,230],"beta-lactam":[14,43,46,54,106,134,151,160,191,232],"antibiotics.":[15,44,135],"One":[16],"is":[17,27,37,70,103,228],"production":[18],"beta-lactamases,":[20],"hydrolytic":[21],"enzymes,":[23],"and":[24,60,75,95,201],"the":[25,28,57,62,65,82,86,96,110,119,125,133,139,144,171,198,221,226,231],"other":[26],"expression":[29,69],"penicillin-binding":[31,76],"protein":[32,77,88,97,114,146,156,182,207],"2a":[33],"(PBP":[34],"2a),":[35],"which":[36],"not":[38],"susceptible":[39],"inhibition":[41],"by":[42,132,159],"The":[45,79,113,136,155,181],"sensor-transducer":[47,87],"(BlaR),":[48],"an":[49],"integral":[50],"membrane":[51],"protein,":[52],"binds":[53],"antibiotics":[55,107,152,161],"on":[56,188],"cell":[58,179],"surface":[59],"transduces":[61],"information":[63],"cytoplasm,":[66],"where":[67],"gene":[68,80],"derepressed":[71],"both":[73],"beta-lactamase":[74],"2a.":[78],"sensor":[83],"domain":[84],"(BlaR(S))":[89],"Staphylococcus":[91],"aureus":[92],"was":[93,98,115,174],"cloned,":[94],"purified":[99],"homogeneity.":[101],"It":[102],"shown":[104,116],"that":[105,123,195],"covalently":[108],"modify":[109],"BlaR(S)":[111,145],"protein.":[112],"contain":[118],"unusual":[120],"carboxylated":[121],"lysine":[122],"activates":[124],"active":[126],"site":[127],"serine":[128],"residue":[129],"acylation":[131,158,208],"details":[137],"kinetics":[140],"interactions":[142],"with":[147,162,190],"series":[149],"were":[153],"investigated.":[154],"undergoes":[157,183],"microscopic":[163],"rate":[164],"constants":[165],"(k(2))":[166],"1-26":[168],"s(-1),":[169],"yet":[170],"deacylation":[172],"process":[173,194],"essentially":[175],"irreversible":[176],"within":[177],"one":[178],"cycle.":[180],"significant":[185],"conformational":[186,213],"change":[187],"binding":[189],"antibiotics,":[192],"commences":[196],"at":[197],"preacylation":[199],"complex":[200],"reaches":[202],"its":[203],"full":[204],"effect":[205],"after":[206],"has":[209],"been":[210],"accomplished.":[211],"These":[212],"changes":[214],"are":[215],"likely":[216],"be":[218],"central":[219],"signal":[222],"transduction":[223],"events":[224],"when":[225],"organism":[227],"exposed":[229],"antibiotic.":[233]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}