{"id":"https://openalex.org/W2064090413","doi":"https://doi.org/10.1016/s0166-6851(99)00019-5","title":"Isolation and characterization of a subtractive library enriched for developmentally regulated transcripts expressed during encystation of Toxoplasma gondii","display_name":"Isolation and characterization of a subtractive library enriched for developmentally regulated transcripts expressed during encystation of Toxoplasma gondii","publication_year":1999,"publication_date":"1999-04-01","ids":{"openalex":"https://openalex.org/W2064090413","doi":"https://doi.org/10.1016/s0166-6851(99)00019-5","mag":"2064090413","pmid":"https://pubmed.ncbi.nlm.nih.gov/10340486"},"language":"en","primary_location":{"id":"doi:10.1016/s0166-6851(99)00019-5","doi":"https://doi.org/10.1016/s0166-6851(99)00019-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0166-6851(99)00019-5","pdf_url":null,"source":{"id":"https://openalex.org/S144099348","display_name":"Molecular and Biochemical Parasitology","issn_l":"0166-6851","issn":["0166-6851","1872-9428"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Biochemical Parasitology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029575693","display_name":"Bilal Yahiaoui","orcid":"https://orcid.org/0000-0003-0823-9860","observed_orcids":["https://orcid.org/0000-0003-0823-9860"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Bilel Yahiaoui","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, Villeneuve d'Ascq, France","Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, Villeneuve d'Ascq, France","institution_ids":["https://openalex.org/I154526488"]},{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006795782","display_name":"Florence S. Dzierszinski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Florence Dzierszinski","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035490675","display_name":"Annie Bernigaud","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Annie Bernigaud","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108085386","display_name":"Christian Slomianny","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Christian Slomianny","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108258053","display_name":"Daniel Camus","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Daniel Camus","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025126447","display_name":"Stanislas Tomavo","orcid":"https://orcid.org/0000-0003-2435-1684","observed_orcids":["https://orcid.org/0000-0003-2435-1684"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Stanislas Tomavo","raw_affiliation_strings":["Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 42, Domaine du CERTIA, 369 rue Jules Guesde, 59 651 Villeneuve d\u2019Ascq Cedex, France","institution_ids":["https://openalex.org/I154526488"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5025126447"],"corresponding_institution_ids":["https://openalex.org/I154526488"],"apc_list":{"value":3460,"currency":"USD","value_usd":3460},"apc_paid":null,"fwci":6.3715,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.97273959,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"99","issue":"2","first_page":"223","last_page":"235"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11155","display_name":"Toxoplasma gondii Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11155","display_name":"Toxoplasma gondii Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12691","display_name":"Coccidia and coccidiosis research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/toxoplasma-gondii","display_name":"Toxoplasma gondii","score":1.0},{"id":"https://openalex.org/keywords/cyst-formation","display_name":"cyst formation","score":0.996999979019165},{"id":"https://openalex.org/keywords/bradyzoite-differentiation","display_name":"bradyzoite differentiation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/subtractive-cdna-library","display_name":"subtractive cDNA library","score":0.9570000171661377},{"id":"https://openalex.org/keywords/gene-expression-regulation","display_name":"gene expression regulation","score":0.6620000004768372},{"id":"https://openalex.org/keywords/molecular-chaperones","display_name":"molecular chaperones","score":0.7440000176429749},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.7689999938011169},{"id":"https://openalex.org/keywords/phosphatidylinositol-synthase","display_name":"phosphatidylinositol synthase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/glucose-6-phosphate-isomerase","display_name":"phosphoglucose isomerase","score":0.9950000047683716},{"id":"https://openalex.org/keywords/enolase","display_name":"enolase","score":0.8399999737739563}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9358813762664795},{"id":"https://openalex.org/C2778128430","wikidata":"https://www.wikidata.org/wiki/Q131003","display_name":"Toxoplasma gondii","level":3,"score":0.8338719606399536},{"id":"https://openalex.org/C196436469","wikidata":"https://www.wikidata.org/wiki/Q6587700","display_name":"Suppression subtractive hybridization","level":5,"score":0.6844246983528137},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6814175248146057},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6731452941894531},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.5700060129165649},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5453504920005798},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.5302025079727173},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.43289998173713684},{"id":"https://openalex.org/C188618488","wikidata":"https://www.wikidata.org/wiki/Q24776639","display_name":"Enolase","level":3,"score":0.4317828416824341},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3880499005317688},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3486486077308655},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.10824939608573914},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008858","descriptor_name":"Microscopy, Phase-Contrast","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014122","descriptor_name":"Toxoplasma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014122","descriptor_name":"Toxoplasma","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017125","descriptor_name":"Genes, Protozoan","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0166-6851(99)00019-5","doi":"https://doi.org/10.1016/s0166-6851(99)00019-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0166-6851(99)00019-5","pdf_url":null,"source":{"id":"https://openalex.org/S144099348","display_name":"Molecular and Biochemical Parasitology","issn_l":"0166-6851","issn":["0166-6851","1872-9428"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and Biochemical Parasitology","raw_type":"journal-article"},{"id":"pmid:10340486","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10340486","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular and biochemical parasitology","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320307875","display_name":"Styrelsen f\u00f6r Internationellt Utvecklingssamarbete","ror":"https://ror.org/01fn7me06"},{"id":"https://openalex.org/F4320310985","display_name":"Sidaction","ror":"https://ror.org/04z6q9z55"},{"id":"https://openalex.org/F4320320883","display_name":"Agence Nationale de la Recherche","ror":"https://ror.org/00rbzpz17"},{"id":"https://openalex.org/F4320321589","display_name":"Fondation pour la Recherche M\u00e9dicale","ror":"https://ror.org/04w6kn183"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1484320524","https://openalex.org/W1545910465","https://openalex.org/W1554662504","https://openalex.org/W1580968716","https://openalex.org/W1889963210","https://openalex.org/W1892700069","https://openalex.org/W1911235289","https://openalex.org/W1929629802","https://openalex.org/W1977500078","https://openalex.org/W1986815480","https://openalex.org/W1993158258","https://openalex.org/W1997093700","https://openalex.org/W2016749399","https://openalex.org/W2018543079","https://openalex.org/W2020188860","https://openalex.org/W2034752048","https://openalex.org/W2037836672","https://openalex.org/W2038836483","https://openalex.org/W2044929411","https://openalex.org/W2049062612","https://openalex.org/W2052538813","https://openalex.org/W2055043387","https://openalex.org/W2055812927","https://openalex.org/W2065654101","https://openalex.org/W2079084833","https://openalex.org/W2080037386","https://openalex.org/W2084821604","https://openalex.org/W2085088144","https://openalex.org/W2099518305","https://openalex.org/W2106093190","https://openalex.org/W2111805246","https://openalex.org/W2137724783","https://openalex.org/W2172006456","https://openalex.org/W2259991476","https://openalex.org/W3025190738","https://openalex.org/W7095735447"],"related_works":["https://openalex.org/W4401657493","https://openalex.org/W1889963210","https://openalex.org/W2102705130","https://openalex.org/W2011566945","https://openalex.org/W2549268157","https://openalex.org/W2154800468","https://openalex.org/W1760381734","https://openalex.org/W2137724783","https://openalex.org/W2987232061","https://openalex.org/W2876450810"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}