{"id":"https://openalex.org/W2064006240","doi":"https://doi.org/10.1007/bf02921260","title":"Isolation and culture of suspension-derived protoplasts ofBeta vulgaris L.","display_name":"Isolation and culture of suspension-derived protoplasts ofBeta vulgaris L.","publication_year":1994,"publication_date":"1994-01-01","ids":{"openalex":"https://openalex.org/W2064006240","doi":"https://doi.org/10.1007/bf02921260","mag":"2064006240"},"language":"en","primary_location":{"id":"doi:10.1007/bf02921260","doi":"https://doi.org/10.1007/bf02921260","is_oa":true,"landing_page_url":"https://doi.org/10.1007/bf02921260","pdf_url":"http://bp.ueb.cas.cz/doi/10.1007/BF02921260.pdf","source":{"id":"https://openalex.org/S56909845","display_name":"Biologia Plantarum","issn_l":"0006-3134","issn":["0006-3134","1573-8264"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biologia plantarum","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://bp.ueb.cas.cz/doi/10.1007/BF02921260.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091612844","display_name":"Anna Majewska-Sawka","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. Majewska-Sawka","raw_affiliation_strings":["Institute for Plant Breeding and Acclimatization, Bydgoszcz, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Plant Breeding and Acclimatization, Bydgoszcz, Poland","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"H. Nakashima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H. Nakashima","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Sapporo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Sapporo, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"last","author":{"id":null,"display_name":"K. Mori","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K. Mori","raw_affiliation_strings":["Faculty of Agriculture, Hokkaido University, Sapporo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Hokkaido University, Sapporo, Japan","institution_ids":["https://openalex.org/I205349734"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3140,"currency":"USD","value_usd":3140},"apc_paid":null,"fwci":0.5078,"has_fulltext":true,"cited_by_count":6,"citation_normalized_percentile":{"value":0.67164769,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"36","issue":"1","first_page":"9","last_page":"13"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-vulgaris","display_name":"Beta vulgaris","score":0.8100000023841858},{"id":"https://openalex.org/keywords/sugar-beet","display_name":"sugar beet","score":0.921999990940094},{"id":"https://openalex.org/keywords/protoplast-isolation","display_name":"protoplast isolation","score":0.9800000190734863},{"id":"https://openalex.org/keywords/protoplast-culture","display_name":"protoplast culture","score":0.9010000228881836},{"id":"https://openalex.org/keywords/suspension-culture","display_name":"suspension culture","score":0.5709999799728394},{"id":"https://openalex.org/keywords/agarose-immobilization","display_name":"agarose immobilization","score":0.8640000224113464},{"id":"https://openalex.org/keywords/callus-proliferation","display_name":"callus proliferation","score":0.546999990940094},{"id":"https://openalex.org/keywords/benzylaminopurine","display_name":"benzylaminopurine","score":0.8119999766349792},{"id":"https://openalex.org/keywords/naphthaleneacetic-acid","display_name":"naphthaleneacetic acid","score":0.9789999723434448},{"id":"https://openalex.org/keywords/plant-regeneration","display_name":"plant regeneration","score":0.6169999837875366},{"id":"https://openalex.org/keywords/organogenesis","display_name":"organogenesis","score":0.7620000243186951}],"concepts":[{"id":"https://openalex.org/C173749725","wikidata":"https://www.wikidata.org/wiki/Q1126500","display_name":"Protoplast","level":2,"score":0.9792919158935547},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.8002649545669556},{"id":"https://openalex.org/C105409712","wikidata":"https://www.wikidata.org/wiki/Q1426202","display_name":"Hypocotyl","level":2,"score":0.7361511588096619},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.654168426990509},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5589061379432678},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.5345925688743591},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5279050469398499},{"id":"https://openalex.org/C30503417","wikidata":"https://www.wikidata.org/wiki/Q958938","display_name":"Organogenesis","level":3,"score":0.49439096450805664},{"id":"https://openalex.org/C2776127477","wikidata":"https://www.wikidata.org/wiki/Q17093220","display_name":"Plating efficiency","level":3,"score":0.48762574791908264},{"id":"https://openalex.org/C2778660310","wikidata":"https://www.wikidata.org/wiki/Q177998","display_name":"Agar","level":3,"score":0.43487000465393066},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.32120710611343384},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2209523320198059},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.09019190073013306},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0542130172252655},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.04553210735321045},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/bf02921260","doi":"https://doi.org/10.1007/bf02921260","is_oa":true,"landing_page_url":"https://doi.org/10.1007/bf02921260","pdf_url":"http://bp.ueb.cas.cz/doi/10.1007/BF02921260.pdf","source":{"id":"https://openalex.org/S56909845","display_name":"Biologia Plantarum","issn_l":"0006-3134","issn":["0006-3134","1573-8264"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biologia plantarum","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/bf02921260","doi":"https://doi.org/10.1007/bf02921260","is_oa":true,"landing_page_url":"https://doi.org/10.1007/bf02921260","pdf_url":"http://bp.ueb.cas.cz/doi/10.1007/BF02921260.pdf","source":{"id":"https://openalex.org/S56909845","display_name":"Biologia Plantarum","issn_l":"0006-3134","issn":["0006-3134","1573-8264"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biologia plantarum","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.40450000762939453,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2064006240.pdf","grobid_xml":"https://content.openalex.org/works/W2064006240.grobid-xml"},"referenced_works_count":9,"referenced_works":["https://openalex.org/W348150228","https://openalex.org/W1994796962","https://openalex.org/W1996390759","https://openalex.org/W2014925614","https://openalex.org/W2021159618","https://openalex.org/W2049073147","https://openalex.org/W2059938589","https://openalex.org/W2081833459","https://openalex.org/W2783647657"],"related_works":["https://openalex.org/W7112604089","https://openalex.org/W1996390759","https://openalex.org/W2021159618","https://openalex.org/W2014925614","https://openalex.org/W2078383321","https://openalex.org/W2071830929","https://openalex.org/W119855643","https://openalex.org/W2032999826","https://openalex.org/W2066140681","https://openalex.org/W1985558160"],"abstract_inverted_index":{"Sugar":[0],"beet":[1],"protoplasts":[2,102],"(Beta":[3],"vulgaris":[4],"L.)":[5],"were":[6,33],"isolated":[7],"from":[8],"hypocotyl-derived":[9],"suspension":[10],"cells":[11],"and":[12,17,27,40,85],"cultured":[13],"on":[14],"modified":[15],"Murashige":[16],"Skoog":[18],"medium":[19,45,48],"supplemented":[20],"with":[21,118],"5":[22],"\u03bcM":[23,29],"naphthaleneacetic":[24],"acid":[25],"(NAA)":[26],"2":[28],"6-benzyl-aminopurine":[30],"(BAP).":[31],"Protoplasts":[32],"plated":[34],"at":[35,53,137],"a":[36],"density":[37],"1.0-1.5\u0102\u0097105":[38],"cm-3":[39],"incubated":[41],"in":[42,47,55,74,132],"either":[43],"liquid":[44],"or":[46],"solidified":[49],"by":[50],"1.2%":[51],"agarose,":[52],"25\u00c2\u00b0C":[54],"the":[56,66,72,99,138],"dark.":[57],"Comparison":[58],"of":[59,62,95,101,112,121],"two":[60,93],"methods":[61],"culture":[63],"unequivocally":[64],"showed":[65],"second":[67],"to":[68,77,105,115],"be":[69,78],"superior.":[70],"Immobilizing":[71],"protoplast":[73,83],"agarose":[75],"proved":[76],"essential":[79],"for":[80],"obtaining":[81],"sustained":[82],"division":[84],"reproducible":[86],"colony":[87],"formation.":[88],"The":[89],"plating":[90],"efficiency":[91],"after":[92],"weeks":[94],"culture,":[96],"expressed":[97],"as":[98],"percentage":[100],"which":[103],"developed":[104],"form":[106],"colonies,":[107],"reached":[108],"40%.":[109],"Subsequent":[110],"subcultures":[111],"protoplast-derived":[113],"callus":[114,135],"regeneration":[116],"media":[117],"different":[119],"concentrations":[120],"BAP":[122],"(5":[123],"\u03bcM,":[124,126,128],"10":[125],"20":[127],"30":[129],"\u03bcM)":[130],"resulted":[131],"very":[133],"good":[134],"proliferation":[136],"three":[139],"lowest":[140],"concentrations,":[141],"although":[142],"organogenesis":[143],"was":[144],"not":[145],"achieved.":[146]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
