{"id":"https://openalex.org/W2063659241","doi":"https://doi.org/10.1073/pnas.75.8.3688","title":"The ovalbumin gene: Cloning of the natural gene","display_name":"The ovalbumin gene: Cloning of the natural gene","publication_year":1978,"publication_date":"1978-08-01","ids":{"openalex":"https://openalex.org/W2063659241","doi":"https://doi.org/10.1073/pnas.75.8.3688","mag":"2063659241","pmid":"https://pubmed.ncbi.nlm.nih.gov/358194","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/392851"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.75.8.3688","doi":"https://doi.org/10.1073/pnas.75.8.3688","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.8.3688","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/392851","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110185434","display_name":"Savio L.C. Woo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Savio L. C. Woo","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002591205","display_name":"Achilles Dugaiczyk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Achilles Dugaiczyk","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087200092","display_name":"Ming\u2010Jer Tsai","orcid":"https://orcid.org/0000-0001-6287-908X","observed_orcids":["https://orcid.org/0000-0001-6287-908X"]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ming-Jer Tsai","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105530526","display_name":"Eugene C. Lai","orcid":"https://orcid.org/0000-0002-4826-1328","observed_orcids":["https://orcid.org/0000-0002-4826-1328"]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eugene C. Lai","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074183604","display_name":"James F. Catterall","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James F. Catterall","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057978718","display_name":"Bert W. O\u2019Malley","orcid":"https://orcid.org/0000-0003-3332-1399","observed_orcids":["https://orcid.org/0000-0003-3332-1399"]},"institutions":[{"id":"https://openalex.org/I181547552","display_name":"Baylor College of Medicine","ror":"https://ror.org/02pttbw34","country_code":"US","type":"education","lineage":["https://openalex.org/I181547552","https://openalex.org/I2801539370"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bert W. O'Malley","raw_affiliation_strings":["Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030","institution_ids":["https://openalex.org/I181547552"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I181547552"],"apc_list":null,"apc_paid":null,"fwci":6.2576,"has_fulltext":false,"cited_by_count":73,"citation_normalized_percentile":{"value":0.98028093,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"75","issue":"8","first_page":"3688","last_page":"3692"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9160000085830688,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9160000085830688,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.01140000019222498,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ovalbumin-gene","display_name":"ovalbumin gene","score":0.9929999709129333},{"id":"https://openalex.org/keywords/chickens","display_name":"chickens","score":0.5820000171661377},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.6790000200271606},{"id":"https://openalex.org/keywords/introns","display_name":"introns","score":0.7440000176429749},{"id":"https://openalex.org/keywords/gene-expression","display_name":"gene expression","score":0.6679999828338623},{"id":"https://openalex.org/keywords/restriction-endonuclease-sites","display_name":"restriction endonuclease sites","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C130123407","wikidata":"https://www.wikidata.org/wiki/Q417754","display_name":"EcoRI","level":4,"score":0.7815303206443787},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.756586492061615},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.750385046005249},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.7239922285079956},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6035042405128479},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5538573861122131},{"id":"https://openalex.org/C2776946954","wikidata":"https://www.wikidata.org/wiki/Q420291","display_name":"Ovalbumin","level":3,"score":0.48132336139678955},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.4738472104072571},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.45456957817077637},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4520636796951294},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4424344003200531},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.4389427900314331},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.4261315166950226},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.39908504486083984},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.336211234331131},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.31365644931793213},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.16219568252563477},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010047","descriptor_name":"Ovalbumin","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010047","descriptor_name":"Ovalbumin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.75.8.3688","doi":"https://doi.org/10.1073/pnas.75.8.3688","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.8.3688","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:392851","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/392851","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:358194","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/358194","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:392851","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/392851","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W1515245060","https://openalex.org/W1538764721","https://openalex.org/W1965296752","https://openalex.org/W1986027590","https://openalex.org/W1992228474","https://openalex.org/W1995420892","https://openalex.org/W1995951599","https://openalex.org/W2023221443","https://openalex.org/W2024983898","https://openalex.org/W2028030581","https://openalex.org/W2028488176","https://openalex.org/W2035520923","https://openalex.org/W2037344742","https://openalex.org/W2042357352","https://openalex.org/W2050077441","https://openalex.org/W2060042110","https://openalex.org/W2063450941","https://openalex.org/W2068332356","https://openalex.org/W2078281648","https://openalex.org/W2088913344"],"related_works":["https://openalex.org/W2060042110","https://openalex.org/W66671206","https://openalex.org/W2043912171","https://openalex.org/W2046596653","https://openalex.org/W2122953356","https://openalex.org/W2073291890","https://openalex.org/W67790689","https://openalex.org/W2092722484","https://openalex.org/W2079381369","https://openalex.org/W2088293703"],"abstract_inverted_index":{"The":[0,56,171],"structural":[1,28,134,211],"ovalbumin":[2,29,35,135],"DNA":[3,30,76,95,137,182,196],"sequences":[4,173,219],"are":[5,9],"not":[6,25],"contiguous":[7],"and":[8,44,103,183,198],"separated":[10],"by":[11,50,82,96,163,223],"multiple":[12],"\"intervening":[13,54],"regions\"":[14],"in":[15,48,80,139,155,203],"native":[16],"chicken":[17,94,169,181,195],"DNA.":[18,170],"EcoRI,":[19],"a":[20,97,121,127],"restriction":[21,150,164],"endonuclease":[22,151],"that":[23],"does":[24],"cleave":[26],"the":[27,33,70,84,132,148,156,210,214,217],"sequences,":[31,197,213],"digests":[32],"natural":[34],"gene":[36,207,212],"into":[37],"three":[38],"distinct":[39],"fragments":[40],"of":[41,66,99,110,118,126,166,216],"2.4,":[42],"1.8,":[43],"9.5":[45],"kilobase":[46],"pairs":[47,65],"length":[49],"cleaving":[51],"within":[52],"these":[53,140],"regions.\"":[55],"2.4-kilobase":[57,157],"pair":[58,158],"fragment":[59,77,125,160],"contains":[60],"only":[61],"about":[62],"450":[63],"nucleotide":[64],"coding":[67],"sequence,":[68],"with":[69,120,178],"rest":[71],"being":[72],"intervening":[73,172,218],"sequences.":[74],"This":[75],"was":[78,144],"cloned":[79],"bacteria":[81],"using":[83],"certified":[85],"EK2":[86],"vector":[87],"lambdagtWES.lambdaB":[88],"after":[89,187],"enrichment":[90],"from":[91],"total":[92,167,180],"EcoRI-digested":[93],"combination":[98],"RPC-5":[100],"column":[101],"chromatography":[102],"preparative":[104],"agarose":[105],"gel":[106],"electrophoresis.":[107],"Five":[108],"out":[109],"approximately":[111],"20,000":[112],"recombinant":[113,128,141],"phage":[114],"plaques":[115],"were":[116,174,190],"capable":[117],"hybridizing":[119],"(32)P-labeled":[122],"Hha":[123],"I":[124],"plasmid":[129],"pOV230":[130],"containing":[131],"entire":[133],"gene.":[136],"amplified":[138],"phages,":[142],"lambdagtWES.OV2.4,":[143],"shown":[145],"to":[146,176,192,200],"contain":[147],"same":[149],"cleavage":[152],"sites":[153],"as":[154],"EcoRI":[159],"previously":[161],"determined":[162],"mapping":[165],"genomic":[168],"allowed":[175],"hybridize":[177],"excess":[179],"oviduct":[184],"nuclear":[185],"RNA":[186],"nick-translation.":[188],"They":[189],"found":[191],"be":[193,201],"unique":[194],"appeared":[199],"transcribed":[202],"their":[204],"entireties":[205],"during":[206],"expression.":[208],"Like":[209],"expression":[215],"is":[220],"also":[221],"inducible":[222],"steroid":[224],"hormones.":[225]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}