{"id":"https://openalex.org/W2063350474","doi":"https://doi.org/10.1073/pnas.0505650102","title":"Methanococcus vannielii selenium-binding protein (SeBP): Chemical reactivity of recombinant SeBP produced in Escherichia coli","display_name":"Methanococcus vannielii selenium-binding protein (SeBP): Chemical reactivity of recombinant SeBP produced in Escherichia coli","publication_year":2005,"publication_date":"2005-08-15","ids":{"openalex":"https://openalex.org/W2063350474","doi":"https://doi.org/10.1073/pnas.0505650102","mag":"2063350474","pmid":"https://pubmed.ncbi.nlm.nih.gov/16103372","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1189349"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.0505650102","doi":"https://doi.org/10.1073/pnas.0505650102","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0505650102","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC1189349","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072536402","display_name":"Kemberly Gayle Patteson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kemberly G. Patteson","raw_affiliation_strings":["Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012","Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50 Room 2120, 50 South Drive, Bethesda, MD 20892-8012, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]},{"raw_affiliation_string":"Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50 Room 2120, 50 South Drive, Bethesda, MD 20892-8012, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Neel Trivedi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Neel Trivedi","raw_affiliation_strings":["Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111390096","display_name":"Thressa Campbell Stadtman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Thressa C. Stadtman","raw_affiliation_strings":["Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111390096"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"],"apc_list":null,"apc_paid":null,"fwci":1.0652,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.75307825,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"102","issue":"34","first_page":"12029","last_page":"12034"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11107","display_name":"Selenium in Biological Systems","score":0.9258000254631042,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11107","display_name":"Selenium in Biological Systems","score":0.9258000254631042,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11931","display_name":"Organoselenium and organotellurium chemistry","score":0.06030000001192093,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.006399999838322401,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/selenium-binding-protein","display_name":"selenium-binding proteins","score":0.9850000143051147},{"id":"https://openalex.org/keywords/methanococcus-vannielii","display_name":"Methanococcus vannielii","score":0.996999979019165},{"id":"https://openalex.org/keywords/selenite-reduction","display_name":"selenite reduction","score":0.781000018119812},{"id":"https://openalex.org/keywords/glutathione","display_name":"glutathione","score":0.6549999713897705},{"id":"https://openalex.org/keywords/selenodiglutathione","display_name":"selenodiglutathione","score":0.9599999785423279},{"id":"https://openalex.org/keywords/cysteine-thiol-groups","display_name":"cysteine thiol groups","score":0.7049999833106995},{"id":"https://openalex.org/keywords/protein-reactivity","display_name":"protein reactivity","score":0.6119999885559082},{"id":"https://openalex.org/keywords/recombinant-protein","display_name":"recombinant protein","score":0.5370000004768372},{"id":"https://openalex.org/keywords/methanogenic-archaea","display_name":"methanogenic archaea","score":0.5270000100135803}],"concepts":[{"id":"https://openalex.org/C553756173","wikidata":"https://www.wikidata.org/wiki/Q876","display_name":"Selenium","level":2,"score":0.8580183982849121},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.8533124923706055},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.7580304145812988},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6739718914031982},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5914536714553833},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.572373628616333},{"id":"https://openalex.org/C2778886173","wikidata":"https://www.wikidata.org/wiki/Q53832","display_name":"Tetramer","level":3,"score":0.5283214449882507},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5109571814537048},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5107417106628418},{"id":"https://openalex.org/C2781324293","wikidata":"https://www.wikidata.org/wiki/Q220410","display_name":"Thiol","level":2,"score":0.4746930003166199},{"id":"https://openalex.org/C2776515853","wikidata":"https://www.wikidata.org/wiki/Q2191895","display_name":"Iodoacetamide","level":4,"score":0.4678443372249603},{"id":"https://openalex.org/C2781014771","wikidata":"https://www.wikidata.org/wiki/Q413961","display_name":"Tris","level":2,"score":0.46295860409736633},{"id":"https://openalex.org/C2777824588","wikidata":"https://www.wikidata.org/wiki/Q414783","display_name":"Dithiothreitol","level":3,"score":0.41212207078933716},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.352008581161499},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25410225987434387},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12647497653961182},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0740104615688324}],"mesh":[{"descriptor_ui":"D002152","descriptor_name":"Calorimetry, Differential Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005978","descriptor_name":"Glutathione","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010720","descriptor_name":"Phosphines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013054","descriptor_name":"Spectrophotometry, Atomic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017017","descriptor_name":"Methanococcus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018038","descriptor_name":"Sodium Selenite","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019791","descriptor_name":"Guanidine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051137","descriptor_name":"Selenium-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.0505650102","doi":"https://doi.org/10.1073/pnas.0505650102","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.0505650102","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:168375","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1189349","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:16103372","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16103372","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Comparative Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:1189349","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1189349/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:168375","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1189349","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1501074197","https://openalex.org/W1534698434","https://openalex.org/W1547929361","https://openalex.org/W1647577587","https://openalex.org/W1973210959","https://openalex.org/W1974686816","https://openalex.org/W1984174807","https://openalex.org/W1984198411","https://openalex.org/W1991600890","https://openalex.org/W2006370393","https://openalex.org/W2014101523","https://openalex.org/W2025035141","https://openalex.org/W2027496568","https://openalex.org/W2034760307","https://openalex.org/W2041655473","https://openalex.org/W2050122375","https://openalex.org/W2054136959","https://openalex.org/W2055422392","https://openalex.org/W2064373917","https://openalex.org/W2066198366","https://openalex.org/W2070884923","https://openalex.org/W2075954862","https://openalex.org/W2076218928","https://openalex.org/W2106741229","https://openalex.org/W2122753552","https://openalex.org/W2147038505","https://openalex.org/W2152302193","https://openalex.org/W2156033464","https://openalex.org/W2315698350"],"related_works":["https://openalex.org/W1979080837","https://openalex.org/W2055422392","https://openalex.org/W4383070183","https://openalex.org/W7212131380","https://openalex.org/W2763415281","https://openalex.org/W2025919734","https://openalex.org/W2089404152","https://openalex.org/W2025035141","https://openalex.org/W2014101523","https://openalex.org/W2051690490"],"abstract_inverted_index":{"A":[0],"selenium-binding":[1],"protein":[2,121],"(SeBP)":[3],"from":[4,161],"Methanococcus":[5],"vannielii":[6],"was":[7,13],"recently":[8],"identified,":[9],"and":[10,15,35,47,79,117,156,163,216],"its":[11],"gene":[12],"isolated":[14,68,119],"overexpressed":[16],"in":[17,69,97,188,210],"Escherichia":[18],"coli":[19],"[Self,":[20],"W.":[21],"T.,":[22],"Pierce,":[23],"R.":[24],"&":[25],"Stadtman,":[26],"T.":[27],"C.":[28],"(2004)":[29],"IUBMB":[30],"Life":[31],"56,":[32],"501-507].":[33],"SeBP":[34,37],"recombinant":[36],"(rSeBP)":[38],"migrated":[39],"as":[40,48,107,113,122,184],"approximately":[41,49],"42-kDa":[42],"species":[43,51],"on":[44,52,140],"native":[45],"gels":[46],"33-kDa":[50],"SDS":[53],"gels.":[54],"rSeBP":[55,67,98,183,212],"consists":[56],"of":[57,72,92,103,126,153,171,195,205],"identical":[58],"8.8-kDa":[59],"subunits,":[60],"each":[61,141],"containing":[62],"a":[63,104,174],"single":[64],"cysteine":[65,77,95,136,207],"residue.":[66],"the":[70,93,114,118,144,181,189,196,206],"absence":[71],"reducing":[73],"agents":[74],"contained":[75],"oxidized":[76,94],"(89%)":[78],"very":[80],"little":[81],"bound":[82],"selenium":[83,115,128,217],"(0.05":[84],"eq":[85],"or":[86],"less":[87],"per":[88,129],"subunit).":[89],"Complete":[90],"reduction":[91,215],"residues":[96],"with":[99,147,180,191],"Tris(2-carboxyethyl)phosphine":[100],"required":[101,134],"addition":[102],"denaturant,":[105],"such":[106],"1":[108],"M":[109],"guanidine-hydrochloride.":[110],"With":[111],"selenite":[112,162,214],"source":[116],"reduced":[120,148,182,211],"sole":[123,185],"reductant,":[124],"binding":[125,218],"one":[127,139],"tetramer":[130],"under":[131,219],"anaerobic":[132],"conditions":[133],"four":[135],"thiol":[137,208],"groups,":[138],"subunit.":[142],"In":[143],"corresponding":[145],"reaction,":[146],"glutathione":[149,157],"(GSH),":[150],"equimolar":[151],"amounts":[152],"selenodiglutathione":[154],"(GSSeSG)":[155],"disulfide":[158],"are":[159],"formed":[160],"4":[164],"GSH.":[165],"At":[166],"GSH-to-selenite":[167],"ratios":[168],">4:1,":[169],"conversion":[170,194],"GSSeSG":[172],"to":[173],"perselenide":[175],"derivative,":[176],"GSSe(-),":[177],"occurs.":[178],"However,":[179],"electron":[186],"donor":[187],"reaction":[190],"selenite,":[192],"further":[193],"R-SSeS-R":[197],"product":[198],"apparently":[199],"did":[200],"not":[201],"occur.":[202],"Prior":[203],"alkylation":[204],"groups":[209],"prevented":[213],"comparable":[220],"conditions.":[221]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}