{"id":"https://openalex.org/W2062993128","doi":"https://doi.org/10.1016/0003-2697(86)90412-4","title":"The purification of alphavirus virions and subviral particles using ultrafiltration and gel exclusion chromatography","display_name":"The purification of alphavirus virions and subviral particles using ultrafiltration and gel exclusion chromatography","publication_year":1986,"publication_date":"1986-02-01","ids":{"openalex":"https://openalex.org/W2062993128","doi":"https://doi.org/10.1016/0003-2697(86)90412-4","mag":"2062993128","pmid":"https://pubmed.ncbi.nlm.nih.gov/3008588"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(86)90412-4","doi":"https://doi.org/10.1016/0003-2697(86)90412-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(86)90412-4","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063125085","display_name":"Alan J. Crooks","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alan J. Crooks","raw_affiliation_strings":["Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000331309","display_name":"John Michael Lee","orcid":"https://orcid.org/0000-0001-8473-4674","observed_orcids":["https://orcid.org/0000-0001-8473-4674"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John M. Lee","raw_affiliation_strings":["Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049728312","display_name":"John R. Stephenson","orcid":"https://orcid.org/0000-0002-7902-1837","observed_orcids":["https://orcid.org/0000-0002-7902-1837"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John R. Stephenson","raw_affiliation_strings":["Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vaccine Research and Production Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, United Kingdom","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.1667855,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"152","issue":"2","first_page":"295","last_page":"303"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.5224999785423279,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.5224999785423279,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.4018999934196472,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.05050000175833702,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alphavirus","display_name":"alphavirus","score":0.8299999833106995},{"id":"https://openalex.org/keywords/virus-purification","display_name":"virus purification","score":0.9039999842643738},{"id":"https://openalex.org/keywords/subviral-particles","display_name":"subviral particles","score":0.8180000185966492},{"id":"https://openalex.org/keywords/ultrafiltration","display_name":"ultrafiltration","score":0.968999981880188},{"id":"https://openalex.org/keywords/gel-exclusion-chromatography","display_name":"gel exclusion chromatography","score":0.9950000047683716},{"id":"https://openalex.org/keywords/viral-envelope-proteins","display_name":"viral envelope proteins","score":0.5230000019073486},{"id":"https://openalex.org/keywords/protein-aggregates","display_name":"protein aggregates","score":0.843999981880188},{"id":"https://openalex.org/keywords/bioactivity-retention","display_name":"bioactivity retention","score":0.5450000166893005}],"concepts":[{"id":"https://openalex.org/C54689828","wikidata":"https://www.wikidata.org/wiki/Q2029259","display_name":"Ultracentrifuge","level":2,"score":0.8781527280807495},{"id":"https://openalex.org/C2777474056","wikidata":"https://www.wikidata.org/wiki/Q1111605","display_name":"Alphavirus","level":3,"score":0.8527764081954956},{"id":"https://openalex.org/C50817676","wikidata":"https://www.wikidata.org/wiki/Q7880534","display_name":"Ultrafiltration (renal)","level":2,"score":0.7952347993850708},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.7350125908851624},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7139661312103271},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5899101495742798},{"id":"https://openalex.org/C63179538","wikidata":"https://www.wikidata.org/wiki/Q854422","display_name":"Gel permeation chromatography","level":3,"score":0.5001430511474609},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4338177442550659},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.2715841829776764},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19572210311889648},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1723913550376892},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11942398548126221},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000524","descriptor_name":"Alphavirus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000524","descriptor_name":"Alphavirus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000524","descriptor_name":"Alphavirus","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014462","descriptor_name":"Ultrafiltration","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(86)90412-4","doi":"https://doi.org/10.1016/0003-2697(86)90412-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(86)90412-4","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:3008588","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3008588","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W43302033","https://openalex.org/W1630196113","https://openalex.org/W1966321026","https://openalex.org/W1986447450","https://openalex.org/W1989675515","https://openalex.org/W1994008000","https://openalex.org/W2003415682","https://openalex.org/W2055707570","https://openalex.org/W2063574862","https://openalex.org/W2128794754","https://openalex.org/W2412837260"],"related_works":["https://openalex.org/W2794117416","https://openalex.org/W2992993052","https://openalex.org/W2018079894","https://openalex.org/W2550162486","https://openalex.org/W2943184875","https://openalex.org/W2284001292","https://openalex.org/W7132498100","https://openalex.org/W2380002510","https://openalex.org/W4233985206","https://openalex.org/W2400365758"],"abstract_inverted_index":null,"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}